FastQCFastQC Report
Mon 3 Dec 2018
16hr-r2-TAAGGCGA-TATGCAGT_S4_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename16hr-r2-TAAGGCGA-TATGCAGT_S4_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2859368
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGGT604.9861835E-429.1666123
CGAACGT500.007212206327.99994315
CGTCTAG3550.025.6368891
CCGGATA1454.6136665E-721.7240944
GGACGTG12500.021.5599586
ATCTCGT3000.020.99995870
GACGTGG9200.020.923877
TTGCGAG1205.7215922E-520.4166289
TAGGACG14000.020.2499584
GGTACGA2252.1282176E-1020.22218318
CCACGAA32550.020.21501470
GTACGAG2453.092282E-1119.9999619
TTGACGG3750.019.59996231
GCGAATA6200.019.1935170
TATGCCG1651.5948754E-619.09087249
ACGTCCG1107.4809423E-419.0908732
ATGACCG2801.0913936E-1118.7499645
CTAAGGC49400.018.49186770
ACGTGGA9500.018.4210178
CGGTCAT2701.2369128E-1018.14811135
Produced by FastQC (version 0.11.4)