######################################################################## # LASTZ Panagrellus redivivus/Microworm - panRed1 vs. # Ancylostoma ceylanicum/A. ceylanicum - ancCey1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/panRed1/bed/lastzAncCey1.2015-08-16 cd /hive/data/genomes/panRed1/bed/lastzAncCey1.2015-08-16 cat << '_EOF_' > DEF # panRed1 vs ancCey1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Panagrellus redivivus/Microworm - panRed1 SEQ1_DIR=/hive/data/genomes/panRed1/panRed1.2bit SEQ1_LEN=/hive/data/genomes/panRed1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Ancylostoma ceylanicum/A. ceylanicum - ancCey1 SEQ2_DIR=/hive/data/genomes/ancCey1/ancCey1.2bit SEQ2_LEN=/hive/data/genomes/ancCey1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/panRed1/bed/lastzAncCey1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 3m54.627s cat fb.panRed1.chainAncCey1Link.txt # 5391719 bases of 62228262 (8.664%) in intersection time (doRecipBest.pl -buildDir=`pwd` panRed1 ancCey1) > rbest.log 2>&1 & # real 0m29.421s # and for the swap: mkdir /hive/data/genomes/ancCey1/bed/blastz.panRed1.swap cd /hive/data/genomes/ancCey1/bed/blastz.panRed1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/panRed1/bed/lastzAncCey1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m54.831s cat fb.ancCey1.chainPanRed1Link.txt # 6124054 bases of 300914990 (2.035%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` ancCey1 panRed1) \ > rbest.log 2>&1 # real 0m36.198s ######################################################################## ######################################################################## # LASTZ Panagrellus redivivus/Microworm - panRed1 vs. # Ascaris suum/Pig roundworm - ascSuu1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/panRed1/bed/lastzAscSuu1.2015-08-16 cd /hive/data/genomes/panRed1/bed/lastzAscSuu1.2015-08-16 cat << '_EOF_' > DEF # panRed1 vs ascSuu1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Panagrellus redivivus/Microworm - panRed1 SEQ1_DIR=/hive/data/genomes/panRed1/panRed1.2bit SEQ1_LEN=/hive/data/genomes/panRed1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Ascaris suum/Pig roundworm - ascSuu1 SEQ2_DIR=/hive/data/genomes/ascSuu1/ascSuu1.2bit SEQ2_LEN=/hive/data/genomes/ascSuu1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/panRed1/bed/lastzAscSuu1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 4m47.891s cat fb.panRed1.chainAscSuu1Link.txt # 5186670 bases of 62228262 (8.335%) in intersection time (doRecipBest.pl -buildDir=`pwd` panRed1 ascSuu1) > rbest.log 2>&1 & # real 0m29.309s # and for the swap: mkdir /hive/data/genomes/ascSuu1/bed/blastz.panRed1.swap cd /hive/data/genomes/ascSuu1/bed/blastz.panRed1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/panRed1/bed/lastzAscSuu1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m52.695s cat fb.ascSuu1.chainPanRed1Link.txt # 5417626 bases of 262604320 (2.063%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` ascSuu1 panRed1) \ > rbest.log 2>&1 # real 2m22.973s ######################################################################## ######################################################################## # LASTZ Panagrellus redivivus/Microworm - panRed1 vs. # Brugia malayi/Filarial worm - bruMal2 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/panRed1/bed/lastzBruMal2.2015-08-16 cd /hive/data/genomes/panRed1/bed/lastzBruMal2.2015-08-16 cat << '_EOF_' > DEF # panRed1 vs bruMal2 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Panagrellus redivivus/Microworm - panRed1 SEQ1_DIR=/hive/data/genomes/panRed1/panRed1.2bit SEQ1_LEN=/hive/data/genomes/panRed1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Brugia malayi/Filarial worm - bruMal2 SEQ2_DIR=/hive/data/genomes/bruMal2/bruMal2.2bit SEQ2_LEN=/hive/data/genomes/bruMal2/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/panRed1/bed/lastzBruMal2.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 5m39.461s cat fb.panRed1.chainBruMal2Link.txt # 4076481 bases of 62228262 (6.551%) in intersection time (doRecipBest.pl -buildDir=`pwd` panRed1 bruMal2) > rbest.log 2>&1 & # real 0m27.496s # and for the swap: mkdir /hive/data/genomes/bruMal2/bed/blastz.panRed1.swap cd /hive/data/genomes/bruMal2/bed/blastz.panRed1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/panRed1/bed/lastzBruMal2.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 4m1.607s cat fb.bruMal2.chainPanRed1Link.txt # 4031921 bases of 87424120 (4.612%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` bruMal2 panRed1) \ > rbest.log 2>&1 # real 4m46.316s ######################################################################## ######################################################################## # LASTZ Panagrellus redivivus/Microworm - panRed1 vs. # Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/panRed1/bed/lastzBurXyl1.2015-08-16 cd /hive/data/genomes/panRed1/bed/lastzBurXyl1.2015-08-16 cat << '_EOF_' > DEF # panRed1 vs burXyl1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Panagrellus redivivus/Microworm - panRed1 SEQ1_DIR=/hive/data/genomes/panRed1/panRed1.2bit SEQ1_LEN=/hive/data/genomes/panRed1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 SEQ2_DIR=/hive/data/genomes/burXyl1/burXyl1.2bit SEQ2_LEN=/hive/data/genomes/burXyl1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/panRed1/bed/lastzBurXyl1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 2m47.162s cat fb.panRed1.chainBurXyl1Link.txt # 7863834 bases of 62228262 (12.637%) in intersection time (doRecipBest.pl -buildDir=`pwd` panRed1 burXyl1) > rbest.log 2>&1 & # real 0m32.734s # and for the swap: mkdir /hive/data/genomes/burXyl1/bed/blastz.panRed1.swap cd /hive/data/genomes/burXyl1/bed/blastz.panRed1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/panRed1/bed/lastzBurXyl1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m55.828s cat fb.burXyl1.chainPanRed1Link.txt # 7765061 bases of 73100506 (10.622%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` burXyl1 panRed1) \ > rbest.log 2>&1 # real 0m37.396s ######################################################################## ######################################################################## # LASTZ Panagrellus redivivus/Microworm - panRed1 vs. # Dirofilaria immitis/Dog heartworm - dirImm1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/panRed1/bed/lastzDirImm1.2015-08-16 cd /hive/data/genomes/panRed1/bed/lastzDirImm1.2015-08-16 cat << '_EOF_' > DEF # panRed1 vs dirImm1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Panagrellus redivivus/Microworm - panRed1 SEQ1_DIR=/hive/data/genomes/panRed1/panRed1.2bit SEQ1_LEN=/hive/data/genomes/panRed1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Dirofilaria immitis/Dog heartworm - dirImm1 SEQ2_DIR=/hive/data/genomes/dirImm1/dirImm1.2bit SEQ2_LEN=/hive/data/genomes/dirImm1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/panRed1/bed/lastzDirImm1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 3m12.240s cat fb.panRed1.chainDirImm1Link.txt # 3925418 bases of 62228262 (6.308%) in intersection time (doRecipBest.pl -buildDir=`pwd` panRed1 dirImm1) > rbest.log 2>&1 & # real 0m23.418s # and for the swap: mkdir /hive/data/genomes/dirImm1/bed/blastz.panRed1.swap cd /hive/data/genomes/dirImm1/bed/blastz.panRed1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/panRed1/bed/lastzDirImm1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m57.429s cat fb.dirImm1.chainPanRed1Link.txt # 3764987 bases of 84923352 (4.433%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` dirImm1 panRed1) \ > rbest.log 2>&1 # real 0m31.829s ######################################################################## ######################################################################## # LASTZ Panagrellus redivivus/Microworm - panRed1 vs. # Haemonchus contortus/Barber pole worm - haeCon2 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/panRed1/bed/lastzHaeCon2.2015-08-16 cd /hive/data/genomes/panRed1/bed/lastzHaeCon2.2015-08-16 cat << '_EOF_' > DEF # panRed1 vs haeCon2 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Panagrellus redivivus/Microworm - panRed1 SEQ1_DIR=/hive/data/genomes/panRed1/panRed1.2bit SEQ1_LEN=/hive/data/genomes/panRed1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Haemonchus contortus/Barber pole worm - haeCon2 SEQ2_DIR=/hive/data/genomes/haeCon2/haeCon2.2bit SEQ2_LEN=/hive/data/genomes/haeCon2/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/panRed1/bed/lastzHaeCon2.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 4m31.926s cat fb.panRed1.chainHaeCon2Link.txt # 4849276 bases of 62228262 (7.793%) in intersection time (doRecipBest.pl -buildDir=`pwd` panRed1 haeCon2) > rbest.log 2>&1 & # real 0m26.790s # and for the swap: mkdir /hive/data/genomes/haeCon2/bed/blastz.panRed1.swap cd /hive/data/genomes/haeCon2/bed/blastz.panRed1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/panRed1/bed/lastzHaeCon2.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m37.136s cat fb.haeCon2.chainPanRed1Link.txt # 7180519 bases of 346001353 (2.075%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` haeCon2 panRed1) \ > rbest.log 2>&1 # real 0m59.533s ######################################################################## ######################################################################## # LASTZ Panagrellus redivivus/Microworm - panRed1 vs. # Loa loa/Eye worm - loaLoa1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/panRed1/bed/lastzLoaLoa1.2015-08-16 cd /hive/data/genomes/panRed1/bed/lastzLoaLoa1.2015-08-16 cat << '_EOF_' > DEF # panRed1 vs loaLoa1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Panagrellus redivivus/Microworm - panRed1 SEQ1_DIR=/hive/data/genomes/panRed1/panRed1.2bit SEQ1_LEN=/hive/data/genomes/panRed1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Loa loa/Eye worm - loaLoa1 SEQ2_DIR=/hive/data/genomes/loaLoa1/loaLoa1.2bit SEQ2_LEN=/hive/data/genomes/loaLoa1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/panRed1/bed/lastzLoaLoa1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 3m5.794s cat fb.panRed1.chainLoaLoa1Link.txt # 4157817 bases of 62228262 (6.682%) in intersection time (doRecipBest.pl -buildDir=`pwd` panRed1 loaLoa1) > rbest.log 2>&1 & # real 0m24.303s # and for the swap: mkdir /hive/data/genomes/loaLoa1/bed/blastz.panRed1.swap cd /hive/data/genomes/loaLoa1/bed/blastz.panRed1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/panRed1/bed/lastzLoaLoa1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m47.899s cat fb.loaLoa1.chainPanRed1Link.txt # 3893379 bases of 87533141 (4.448%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` loaLoa1 panRed1) \ > rbest.log 2>&1 # real 0m27.500s ######################################################################## ######################################################################## # LASTZ Panagrellus redivivus/Microworm - panRed1 vs. # Meloidogyne hapla/M. hapla - melHap1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/panRed1/bed/lastzMelHap1.2015-08-16 cd /hive/data/genomes/panRed1/bed/lastzMelHap1.2015-08-16 cat << '_EOF_' > DEF # panRed1 vs melHap1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Panagrellus redivivus/Microworm - panRed1 SEQ1_DIR=/hive/data/genomes/panRed1/panRed1.2bit SEQ1_LEN=/hive/data/genomes/panRed1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Meloidogyne hapla/M. hapla - melHap1 SEQ2_DIR=/hive/data/genomes/melHap1/melHap1.2bit SEQ2_LEN=/hive/data/genomes/melHap1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/panRed1/bed/lastzMelHap1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 3m48.537s cat fb.panRed1.chainMelHap1Link.txt # 3785224 bases of 62228262 (6.083%) in intersection time (doRecipBest.pl -buildDir=`pwd` panRed1 melHap1) > rbest.log 2>&1 & # real 0m21.294s # and for the swap: mkdir /hive/data/genomes/melHap1/bed/blastz.panRed1.swap cd /hive/data/genomes/melHap1/bed/blastz.panRed1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/panRed1/bed/lastzMelHap1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m46.778s cat fb.melHap1.chainPanRed1Link.txt # 3709757 bases of 53017507 (6.997%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` melHap1 panRed1) \ > rbest.log 2>&1 # real 0m24.628s ######################################################################## ######################################################################## # LASTZ Panagrellus redivivus/Microworm - panRed1 vs. # Meloidogyne incognita/M. incognita - melInc2 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/panRed1/bed/lastzMelInc2.2015-08-16 cd /hive/data/genomes/panRed1/bed/lastzMelInc2.2015-08-16 cat << '_EOF_' > DEF # panRed1 vs melInc2 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Panagrellus redivivus/Microworm - panRed1 SEQ1_DIR=/hive/data/genomes/panRed1/panRed1.2bit SEQ1_LEN=/hive/data/genomes/panRed1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Meloidogyne incognita/M. incognita - melInc2 SEQ2_DIR=/hive/data/genomes/melInc2/melInc2.2bit SEQ2_LEN=/hive/data/genomes/melInc2/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/panRed1/bed/lastzMelInc2.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 2m32.578s cat fb.panRed1.chainMelInc2Link.txt # 3300508 bases of 62228262 (5.304%) in intersection time (doRecipBest.pl -buildDir=`pwd` panRed1 melInc2) > rbest.log 2>&1 & # real 0m20.628s # and for the swap: mkdir /hive/data/genomes/melInc2/bed/blastz.panRed1.swap cd /hive/data/genomes/melInc2/bed/blastz.panRed1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/panRed1/bed/lastzMelInc2.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m50.296s cat fb.melInc2.chainPanRed1Link.txt # 4722261 bases of 82114761 (5.751%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` melInc2 panRed1) \ > rbest.log 2>&1 # real 0m25.914s ######################################################################## ######################################################################## # LASTZ Panagrellus redivivus/Microworm - panRed1 vs. # Necator americanus/N. americanus - necAme1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/panRed1/bed/lastzNecAme1.2015-08-16 cd /hive/data/genomes/panRed1/bed/lastzNecAme1.2015-08-16 cat << '_EOF_' > DEF # panRed1 vs necAme1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Panagrellus redivivus/Microworm - panRed1 SEQ1_DIR=/hive/data/genomes/panRed1/panRed1.2bit SEQ1_LEN=/hive/data/genomes/panRed1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Necator americanus/N. americanus - necAme1 SEQ2_DIR=/hive/data/genomes/necAme1/necAme1.2bit SEQ2_LEN=/hive/data/genomes/necAme1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/panRed1/bed/lastzNecAme1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 3m29.801s cat fb.panRed1.chainNecAme1Link.txt # 4673821 bases of 62228262 (7.511%) in intersection time (doRecipBest.pl -buildDir=`pwd` panRed1 necAme1) > rbest.log 2>&1 & # real 0m25.323s # and for the swap: mkdir /hive/data/genomes/necAme1/bed/blastz.panRed1.swap cd /hive/data/genomes/necAme1/bed/blastz.panRed1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/panRed1/bed/lastzNecAme1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m0.651s cat fb.necAme1.chainPanRed1Link.txt # 4809668 bases of 208187215 (2.310%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` necAme1 panRed1) \ > rbest.log 2>&1 # real 0m36.610s ######################################################################## ######################################################################## # LASTZ Panagrellus redivivus/Microworm - panRed1 vs. # Onchocerca volvulus/O. volvulus - oncVol1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/panRed1/bed/lastzOncVol1.2015-08-16 cd /hive/data/genomes/panRed1/bed/lastzOncVol1.2015-08-16 cat << '_EOF_' > DEF # panRed1 vs oncVol1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Panagrellus redivivus/Microworm - panRed1 SEQ1_DIR=/hive/data/genomes/panRed1/panRed1.2bit SEQ1_LEN=/hive/data/genomes/panRed1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Onchocerca volvulus/O. volvulus - oncVol1 SEQ2_DIR=/hive/data/genomes/oncVol1/oncVol1.2bit SEQ2_LEN=/hive/data/genomes/oncVol1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/panRed1/bed/lastzOncVol1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 3m37.782s cat fb.panRed1.chainOncVol1Link.txt # 4117717 bases of 62228262 (6.617%) in intersection time (doRecipBest.pl -buildDir=`pwd` panRed1 oncVol1) > rbest.log 2>&1 & # real 0m37.596s # and for the swap: mkdir /hive/data/genomes/oncVol1/bed/blastz.panRed1.swap cd /hive/data/genomes/oncVol1/bed/blastz.panRed1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/panRed1/bed/lastzOncVol1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m43.313s cat fb.oncVol1.chainPanRed1Link.txt # 3952538 bases of 94327777 (4.190%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` oncVol1 panRed1) \ > rbest.log 2>&1 # real 0m35.765s ######################################################################## ######################################################################## # LASTZ Panagrellus redivivus/Microworm - panRed1 vs. # Pristionchus exspectatus/P. exspectatus - priExs1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/panRed1/bed/lastzPriExs1.2015-08-16 cd /hive/data/genomes/panRed1/bed/lastzPriExs1.2015-08-16 cat << '_EOF_' > DEF # panRed1 vs priExs1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Panagrellus redivivus/Microworm - panRed1 SEQ1_DIR=/hive/data/genomes/panRed1/panRed1.2bit SEQ1_LEN=/hive/data/genomes/panRed1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Pristionchus exspectatus/P. exspectatus - priExs1 SEQ2_DIR=/hive/data/genomes/priExs1/priExs1.2bit SEQ2_LEN=/hive/data/genomes/priExs1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/panRed1/bed/lastzPriExs1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 2m46.291s cat fb.panRed1.chainPriExs1Link.txt # 4233643 bases of 62228262 (6.803%) in intersection time (doRecipBest.pl -buildDir=`pwd` panRed1 priExs1) > rbest.log 2>&1 & # real 0m26.547s # and for the swap: mkdir /hive/data/genomes/priExs1/bed/blastz.panRed1.swap cd /hive/data/genomes/priExs1/bed/blastz.panRed1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/panRed1/bed/lastzPriExs1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m55.237s cat fb.priExs1.chainPanRed1Link.txt # 5165560 bases of 166917800 (3.095%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` priExs1 panRed1) \ > rbest.log 2>&1 # real 0m32.496s ######################################################################## ######################################################################## # LASTZ Panagrellus redivivus/Microworm - panRed1 vs. # Pristionchus pacificus/P. pacificus - priPac3 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/panRed1/bed/lastzPriPac3.2015-08-16 cd /hive/data/genomes/panRed1/bed/lastzPriPac3.2015-08-16 cat << '_EOF_' > DEF # panRed1 vs priPac3 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Panagrellus redivivus/Microworm - panRed1 SEQ1_DIR=/hive/data/genomes/panRed1/panRed1.2bit SEQ1_LEN=/hive/data/genomes/panRed1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Pristionchus pacificus/P. pacificus - priPac3 SEQ2_DIR=/hive/data/genomes/priPac3/priPac3.2bit SEQ2_LEN=/hive/data/genomes/priPac3/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/panRed1/bed/lastzPriPac3.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 4m42.527s cat fb.panRed1.chainPriPac3Link.txt # 4242282 bases of 62228262 (6.817%) in intersection time (doRecipBest.pl -buildDir=`pwd` panRed1 priPac3) > rbest.log 2>&1 & # real 0m29.802s # and for the swap: mkdir /hive/data/genomes/priPac3/bed/blastz.panRed1.swap cd /hive/data/genomes/priPac3/bed/blastz.panRed1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/panRed1/bed/lastzPriPac3.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m31.195s cat fb.priPac3.chainPanRed1Link.txt # 4992757 bases of 153238914 (3.258%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` priPac3 panRed1) \ > rbest.log 2>&1 # real 2m30.726s ######################################################################## ######################################################################## # LASTZ Panagrellus redivivus/Microworm - panRed1 vs. # Trichinella spiralis/Trichinella - triSpi1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/panRed1/bed/lastzTriSpi1.2015-08-16 cd /hive/data/genomes/panRed1/bed/lastzTriSpi1.2015-08-16 cat << '_EOF_' > DEF # panRed1 vs triSpi1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Panagrellus redivivus/Microworm - panRed1 SEQ1_DIR=/hive/data/genomes/panRed1/panRed1.2bit SEQ1_LEN=/hive/data/genomes/panRed1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Trichinella spiralis/Trichinella - triSpi1 SEQ2_DIR=/hive/data/genomes/triSpi1/triSpi1.2bit SEQ2_LEN=/hive/data/genomes/triSpi1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/panRed1/bed/lastzTriSpi1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 3m3.808s cat fb.panRed1.chainTriSpi1Link.txt # 2693261 bases of 62228262 (4.328%) in intersection time (doRecipBest.pl -buildDir=`pwd` panRed1 triSpi1) > rbest.log 2>&1 & # real 0m22.352s # and for the swap: mkdir /hive/data/genomes/triSpi1/bed/blastz.panRed1.swap cd /hive/data/genomes/triSpi1/bed/blastz.panRed1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/panRed1/bed/lastzTriSpi1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m55.107s cat fb.triSpi1.chainPanRed1Link.txt # 2798228 bases of 58558192 (4.779%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` triSpi1 panRed1) \ > rbest.log 2>&1 # real 1m30.662s ######################################################################## ######################################################################## # LASTZ Panagrellus redivivus/Microworm - panRed1 vs. # Trichuris suis/Whipworm - triSui1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/panRed1/bed/lastzTriSui1.2015-08-16 cd /hive/data/genomes/panRed1/bed/lastzTriSui1.2015-08-16 cat << '_EOF_' > DEF # panRed1 vs triSui1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Panagrellus redivivus/Microworm - panRed1 SEQ1_DIR=/hive/data/genomes/panRed1/panRed1.2bit SEQ1_LEN=/hive/data/genomes/panRed1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Trichuris suis/Whipworm - triSui1 SEQ2_DIR=/hive/data/genomes/triSui1/triSui1.2bit SEQ2_LEN=/hive/data/genomes/triSui1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/panRed1/bed/lastzTriSui1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 4m58.602s cat fb.panRed1.chainTriSui1Link.txt # 3005100 bases of 62228262 (4.829%) in intersection time (doRecipBest.pl -buildDir=`pwd` panRed1 triSui1) > rbest.log 2>&1 & # real 0m25.394s # and for the swap: mkdir /hive/data/genomes/triSui1/bed/blastz.panRed1.swap cd /hive/data/genomes/triSui1/bed/blastz.panRed1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/panRed1/bed/lastzTriSui1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 4m31.756s cat fb.triSui1.chainPanRed1Link.txt # 3077479 bases of 71825323 (4.285%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` triSui1 panRed1) \ > rbest.log 2>&1 # real 0m43.276s ########################################################################