######################################################################## # LASTZ Necator americanus/N. americanus - necAme1 vs. # Ascaris suum/Pig roundworm - ascSuu1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/necAme1/bed/lastzAscSuu1.2015-08-16 cd /hive/data/genomes/necAme1/bed/lastzAscSuu1.2015-08-16 cat << '_EOF_' > DEF # necAme1 vs ascSuu1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Necator americanus/N. americanus - necAme1 SEQ1_DIR=/hive/data/genomes/necAme1/necAme1.2bit SEQ1_LEN=/hive/data/genomes/necAme1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Ascaris suum/Pig roundworm - ascSuu1 SEQ2_DIR=/hive/data/genomes/ascSuu1/ascSuu1.2bit SEQ2_LEN=/hive/data/genomes/ascSuu1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/necAme1/bed/lastzAscSuu1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 8m37.442s cat fb.necAme1.chainAscSuu1Link.txt # 6515063 bases of 208187215 (3.129%) in intersection time (doRecipBest.pl -buildDir=`pwd` necAme1 ascSuu1) > rbest.log 2>&1 & # real 0m38.856s # and for the swap: mkdir /hive/data/genomes/ascSuu1/bed/blastz.necAme1.swap cd /hive/data/genomes/ascSuu1/bed/blastz.necAme1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/necAme1/bed/lastzAscSuu1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m6.586s cat fb.ascSuu1.chainNecAme1Link.txt # 6291196 bases of 262604320 (2.396%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` ascSuu1 necAme1) \ > rbest.log 2>&1 # real 0m47.705s ######################################################################## ######################################################################## # LASTZ Necator americanus/N. americanus - necAme1 vs. # Brugia malayi/Filarial worm - bruMal2 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/necAme1/bed/lastzBruMal2.2015-08-17 cd /hive/data/genomes/necAme1/bed/lastzBruMal2.2015-08-17 cat << '_EOF_' > DEF # necAme1 vs bruMal2 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Necator americanus/N. americanus - necAme1 SEQ1_DIR=/hive/data/genomes/necAme1/necAme1.2bit SEQ1_LEN=/hive/data/genomes/necAme1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Brugia malayi/Filarial worm - bruMal2 SEQ2_DIR=/hive/data/genomes/bruMal2/bruMal2.2bit SEQ2_LEN=/hive/data/genomes/bruMal2/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/necAme1/bed/lastzBruMal2.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 5m37.563s cat fb.necAme1.chainBruMal2Link.txt # 5289476 bases of 208187215 (2.541%) in intersection time (doRecipBest.pl -buildDir=`pwd` necAme1 bruMal2) > rbest.log 2>&1 & # real 0m32.442s # and for the swap: mkdir /hive/data/genomes/bruMal2/bed/blastz.necAme1.swap cd /hive/data/genomes/bruMal2/bed/blastz.necAme1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/necAme1/bed/lastzBruMal2.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m35.867s cat fb.bruMal2.chainNecAme1Link.txt # 5414787 bases of 87424120 (6.194%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` bruMal2 necAme1) \ > rbest.log 2>&1 # real 0m37.781s ######################################################################## ######################################################################## # LASTZ Necator americanus/N. americanus - necAme1 vs. # Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/necAme1/bed/lastzBurXyl1.2015-08-17 cd /hive/data/genomes/necAme1/bed/lastzBurXyl1.2015-08-17 cat << '_EOF_' > DEF # necAme1 vs burXyl1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Necator americanus/N. americanus - necAme1 SEQ1_DIR=/hive/data/genomes/necAme1/necAme1.2bit SEQ1_LEN=/hive/data/genomes/necAme1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 SEQ2_DIR=/hive/data/genomes/burXyl1/burXyl1.2bit SEQ2_LEN=/hive/data/genomes/burXyl1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/necAme1/bed/lastzBurXyl1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 4m42.354s cat fb.necAme1.chainBurXyl1Link.txt # 5035251 bases of 208187215 (2.419%) in intersection time (doRecipBest.pl -buildDir=`pwd` necAme1 burXyl1) > rbest.log 2>&1 & # real 0m43.599s # and for the swap: mkdir /hive/data/genomes/burXyl1/bed/blastz.necAme1.swap cd /hive/data/genomes/burXyl1/bed/blastz.necAme1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/necAme1/bed/lastzBurXyl1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m51.794s cat fb.burXyl1.chainNecAme1Link.txt # 4679604 bases of 73100506 (6.402%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` burXyl1 necAme1) \ > rbest.log 2>&1 # real 0m35.511s ######################################################################## ######################################################################## # LASTZ Necator americanus/N. americanus - necAme1 vs. # Dirofilaria immitis/Dog heartworm - dirImm1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/necAme1/bed/lastzDirImm1.2015-08-17 cd /hive/data/genomes/necAme1/bed/lastzDirImm1.2015-08-17 cat << '_EOF_' > DEF # necAme1 vs dirImm1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Necator americanus/N. americanus - necAme1 SEQ1_DIR=/hive/data/genomes/necAme1/necAme1.2bit SEQ1_LEN=/hive/data/genomes/necAme1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Dirofilaria immitis/Dog heartworm - dirImm1 SEQ2_DIR=/hive/data/genomes/dirImm1/dirImm1.2bit SEQ2_LEN=/hive/data/genomes/dirImm1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/necAme1/bed/lastzDirImm1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 5m45.566s cat fb.necAme1.chainDirImm1Link.txt # 5158372 bases of 208187215 (2.478%) in intersection time (doRecipBest.pl -buildDir=`pwd` necAme1 dirImm1) > rbest.log 2>&1 & # real 0m32.728s # and for the swap: mkdir /hive/data/genomes/dirImm1/bed/blastz.necAme1.swap cd /hive/data/genomes/dirImm1/bed/blastz.necAme1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/necAme1/bed/lastzDirImm1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m5.470s cat fb.dirImm1.chainNecAme1Link.txt # 5006918 bases of 84923352 (5.896%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` dirImm1 necAme1) \ > rbest.log 2>&1 # real 0m41.121s ######################################################################## ######################################################################## # LASTZ Necator americanus/N. americanus - necAme1 vs. # Haemonchus contortus/Barber pole worm - haeCon2 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/necAme1/bed/lastzHaeCon2.2015-08-16 cd /hive/data/genomes/necAme1/bed/lastzHaeCon2.2015-08-16 cat << '_EOF_' > DEF # necAme1 vs haeCon2 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Necator americanus/N. americanus - necAme1 SEQ1_DIR=/hive/data/genomes/necAme1/necAme1.2bit SEQ1_LEN=/hive/data/genomes/necAme1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Haemonchus contortus/Barber pole worm - haeCon2 SEQ2_DIR=/hive/data/genomes/haeCon2/haeCon2.2bit SEQ2_LEN=/hive/data/genomes/haeCon2/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/necAme1/bed/lastzHaeCon2.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 13m35.193s cat fb.necAme1.chainHaeCon2Link.txt # 29177064 bases of 208187215 (14.015%) in intersection time (doRecipBest.pl -buildDir=`pwd` necAme1 haeCon2) > rbest.log 2>&1 & # real 1m24.226s # and for the swap: mkdir /hive/data/genomes/haeCon2/bed/blastz.necAme1.swap cd /hive/data/genomes/haeCon2/bed/blastz.necAme1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/necAme1/bed/lastzHaeCon2.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 5m11.770s cat fb.haeCon2.chainNecAme1Link.txt # 42769920 bases of 346001353 (12.361%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` haeCon2 necAme1) \ > rbest.log 2>&1 # real 2m48.118s ######################################################################## ######################################################################## # LASTZ Necator americanus/N. americanus - necAme1 vs. # Loa loa/Eye worm - loaLoa1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/necAme1/bed/lastzLoaLoa1.2015-08-17 cd /hive/data/genomes/necAme1/bed/lastzLoaLoa1.2015-08-17 cat << '_EOF_' > DEF # necAme1 vs loaLoa1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Necator americanus/N. americanus - necAme1 SEQ1_DIR=/hive/data/genomes/necAme1/necAme1.2bit SEQ1_LEN=/hive/data/genomes/necAme1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Loa loa/Eye worm - loaLoa1 SEQ2_DIR=/hive/data/genomes/loaLoa1/loaLoa1.2bit SEQ2_LEN=/hive/data/genomes/loaLoa1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/necAme1/bed/lastzLoaLoa1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 5m16.166s cat fb.necAme1.chainLoaLoa1Link.txt # 5434476 bases of 208187215 (2.610%) in intersection time (doRecipBest.pl -buildDir=`pwd` necAme1 loaLoa1) > rbest.log 2>&1 & # real 0m32.142s # and for the swap: mkdir /hive/data/genomes/loaLoa1/bed/blastz.necAme1.swap cd /hive/data/genomes/loaLoa1/bed/blastz.necAme1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/necAme1/bed/lastzLoaLoa1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m55.240s cat fb.loaLoa1.chainNecAme1Link.txt # 5118183 bases of 87533141 (5.847%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` loaLoa1 necAme1) \ > rbest.log 2>&1 # real 0m34.349s ######################################################################## ######################################################################## # LASTZ Necator americanus/N. americanus - necAme1 vs. # Meloidogyne hapla/M. hapla - melHap1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/necAme1/bed/lastzMelHap1.2015-08-17 cd /hive/data/genomes/necAme1/bed/lastzMelHap1.2015-08-17 cat << '_EOF_' > DEF # necAme1 vs melHap1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Necator americanus/N. americanus - necAme1 SEQ1_DIR=/hive/data/genomes/necAme1/necAme1.2bit SEQ1_LEN=/hive/data/genomes/necAme1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Meloidogyne hapla/M. hapla - melHap1 SEQ2_DIR=/hive/data/genomes/melHap1/melHap1.2bit SEQ2_LEN=/hive/data/genomes/melHap1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/necAme1/bed/lastzMelHap1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 3m47.971s cat fb.necAme1.chainMelHap1Link.txt # 3069272 bases of 208187215 (1.474%) in intersection time (doRecipBest.pl -buildDir=`pwd` necAme1 melHap1) > rbest.log 2>&1 & # real 0m29.791s # and for the swap: mkdir /hive/data/genomes/melHap1/bed/blastz.necAme1.swap cd /hive/data/genomes/melHap1/bed/blastz.necAme1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/necAme1/bed/lastzMelHap1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m47.464s cat fb.melHap1.chainNecAme1Link.txt # 2935804 bases of 53017507 (5.537%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` melHap1 necAme1) \ > rbest.log 2>&1 # real 0m25.202s ######################################################################## ######################################################################## # LASTZ Necator americanus/N. americanus - necAme1 vs. # Meloidogyne incognita/M. incognita - melInc2 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/necAme1/bed/lastzMelInc2.2015-08-17 cd /hive/data/genomes/necAme1/bed/lastzMelInc2.2015-08-17 cat << '_EOF_' > DEF # necAme1 vs melInc2 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Necator americanus/N. americanus - necAme1 SEQ1_DIR=/hive/data/genomes/necAme1/necAme1.2bit SEQ1_LEN=/hive/data/genomes/necAme1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Meloidogyne incognita/M. incognita - melInc2 SEQ2_DIR=/hive/data/genomes/melInc2/melInc2.2bit SEQ2_LEN=/hive/data/genomes/melInc2/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/necAme1/bed/lastzMelInc2.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 4m3.474s cat fb.necAme1.chainMelInc2Link.txt # 2942687 bases of 208187215 (1.413%) in intersection time (doRecipBest.pl -buildDir=`pwd` necAme1 melInc2) > rbest.log 2>&1 & # real 0m31.129s # and for the swap: mkdir /hive/data/genomes/melInc2/bed/blastz.necAme1.swap cd /hive/data/genomes/melInc2/bed/blastz.necAme1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/necAme1/bed/lastzMelInc2.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m51.706s cat fb.melInc2.chainNecAme1Link.txt # 3870419 bases of 82114761 (4.713%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` melInc2 necAme1) \ > rbest.log 2>&1 # real 0m28.679s ######################################################################## ######################################################################## # LASTZ Necator americanus/N. americanus - necAme1 vs. # Onchocerca volvulus/O. volvulus - oncVol1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/necAme1/bed/lastzOncVol1.2015-08-17 cd /hive/data/genomes/necAme1/bed/lastzOncVol1.2015-08-17 cat << '_EOF_' > DEF # necAme1 vs oncVol1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Necator americanus/N. americanus - necAme1 SEQ1_DIR=/hive/data/genomes/necAme1/necAme1.2bit SEQ1_LEN=/hive/data/genomes/necAme1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Onchocerca volvulus/O. volvulus - oncVol1 SEQ2_DIR=/hive/data/genomes/oncVol1/oncVol1.2bit SEQ2_LEN=/hive/data/genomes/oncVol1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/necAme1/bed/lastzOncVol1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 5m15.022s cat fb.necAme1.chainOncVol1Link.txt # 5425777 bases of 208187215 (2.606%) in intersection time (doRecipBest.pl -buildDir=`pwd` necAme1 oncVol1) > rbest.log 2>&1 & # real 0m48.683s # and for the swap: mkdir /hive/data/genomes/oncVol1/bed/blastz.necAme1.swap cd /hive/data/genomes/oncVol1/bed/blastz.necAme1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/necAme1/bed/lastzOncVol1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m50.802s cat fb.oncVol1.chainNecAme1Link.txt # 5222030 bases of 94327777 (5.536%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` oncVol1 necAme1) \ > rbest.log 2>&1 # real 0m45.765s ######################################################################## ######################################################################## # LASTZ Necator americanus/N. americanus - necAme1 vs. # Pristionchus exspectatus/P. exspectatus - priExs1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/necAme1/bed/lastzPriExs1.2015-08-17 cd /hive/data/genomes/necAme1/bed/lastzPriExs1.2015-08-17 cat << '_EOF_' > DEF # necAme1 vs priExs1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Necator americanus/N. americanus - necAme1 SEQ1_DIR=/hive/data/genomes/necAme1/necAme1.2bit SEQ1_LEN=/hive/data/genomes/necAme1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Pristionchus exspectatus/P. exspectatus - priExs1 SEQ2_DIR=/hive/data/genomes/priExs1/priExs1.2bit SEQ2_LEN=/hive/data/genomes/priExs1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/necAme1/bed/lastzPriExs1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 6m48.147s cat fb.necAme1.chainPriExs1Link.txt # 6248632 bases of 208187215 (3.001%) in intersection time (doRecipBest.pl -buildDir=`pwd` necAme1 priExs1) > rbest.log 2>&1 & # real 0m36.156s # and for the swap: mkdir /hive/data/genomes/priExs1/bed/blastz.necAme1.swap cd /hive/data/genomes/priExs1/bed/blastz.necAme1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/necAme1/bed/lastzPriExs1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m4.450s cat fb.priExs1.chainNecAme1Link.txt # 7309633 bases of 166917800 (4.379%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` priExs1 necAme1) \ > rbest.log 2>&1 # real 0m45.953s ######################################################################## ######################################################################## # LASTZ Necator americanus/N. americanus - necAme1 vs. # Pristionchus pacificus/P. pacificus - priPac3 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/necAme1/bed/lastzPriPac3.2015-08-17 cd /hive/data/genomes/necAme1/bed/lastzPriPac3.2015-08-17 cat << '_EOF_' > DEF # necAme1 vs priPac3 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Necator americanus/N. americanus - necAme1 SEQ1_DIR=/hive/data/genomes/necAme1/necAme1.2bit SEQ1_LEN=/hive/data/genomes/necAme1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Pristionchus pacificus/P. pacificus - priPac3 SEQ2_DIR=/hive/data/genomes/priPac3/priPac3.2bit SEQ2_LEN=/hive/data/genomes/priPac3/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/necAme1/bed/lastzPriPac3.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 8m12.906s cat fb.necAme1.chainPriPac3Link.txt # 6353102 bases of 208187215 (3.052%) in intersection time (doRecipBest.pl -buildDir=`pwd` necAme1 priPac3) > rbest.log 2>&1 & # real 0m52.411s # and for the swap: mkdir /hive/data/genomes/priPac3/bed/blastz.necAme1.swap cd /hive/data/genomes/priPac3/bed/blastz.necAme1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/necAme1/bed/lastzPriPac3.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m10.392s cat fb.priPac3.chainNecAme1Link.txt # 7037296 bases of 153238914 (4.592%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` priPac3 necAme1) \ > rbest.log 2>&1 # real 0m47.455s ######################################################################## ######################################################################## # LASTZ Necator americanus/N. americanus - necAme1 vs. # Trichinella spiralis/Trichinella - triSpi1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/necAme1/bed/lastzTriSpi1.2015-08-17 cd /hive/data/genomes/necAme1/bed/lastzTriSpi1.2015-08-17 cat << '_EOF_' > DEF # necAme1 vs triSpi1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Necator americanus/N. americanus - necAme1 SEQ1_DIR=/hive/data/genomes/necAme1/necAme1.2bit SEQ1_LEN=/hive/data/genomes/necAme1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Trichinella spiralis/Trichinella - triSpi1 SEQ2_DIR=/hive/data/genomes/triSpi1/triSpi1.2bit SEQ2_LEN=/hive/data/genomes/triSpi1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/necAme1/bed/lastzTriSpi1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 4m18.800s cat fb.necAme1.chainTriSpi1Link.txt # 2326522 bases of 208187215 (1.118%) in intersection time (doRecipBest.pl -buildDir=`pwd` necAme1 triSpi1) > rbest.log 2>&1 & # real 0m26.185s # and for the swap: mkdir /hive/data/genomes/triSpi1/bed/blastz.necAme1.swap cd /hive/data/genomes/triSpi1/bed/blastz.necAme1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/necAme1/bed/lastzTriSpi1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m45.081s cat fb.triSpi1.chainNecAme1Link.txt # 2400189 bases of 58558192 (4.099%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` triSpi1 necAme1) \ > rbest.log 2>&1 # real 0m24.636s ######################################################################## ######################################################################## # LASTZ Necator americanus/N. americanus - necAme1 vs. # Trichuris suis/Whipworm - triSui1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/necAme1/bed/lastzTriSui1.2015-08-17 cd /hive/data/genomes/necAme1/bed/lastzTriSui1.2015-08-17 cat << '_EOF_' > DEF # necAme1 vs triSui1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Necator americanus/N. americanus - necAme1 SEQ1_DIR=/hive/data/genomes/necAme1/necAme1.2bit SEQ1_LEN=/hive/data/genomes/necAme1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Trichuris suis/Whipworm - triSui1 SEQ2_DIR=/hive/data/genomes/triSui1/triSui1.2bit SEQ2_LEN=/hive/data/genomes/triSui1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/necAme1/bed/lastzTriSui1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 4m26.391s cat fb.necAme1.chainTriSui1Link.txt # 2789972 bases of 208187215 (1.340%) in intersection time (doRecipBest.pl -buildDir=`pwd` necAme1 triSui1) > rbest.log 2>&1 & # real 0m24.360s # and for the swap: mkdir /hive/data/genomes/triSui1/bed/blastz.necAme1.swap cd /hive/data/genomes/triSui1/bed/blastz.necAme1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/necAme1/bed/lastzTriSui1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m45.476s cat fb.triSui1.chainNecAme1Link.txt # 2695997 bases of 71825323 (3.754%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` triSui1 necAme1) \ > rbest.log 2>&1 # real 0m23.220s ########################################################################