######################################################################## # LASTZ Haemonchus contortus/Barber pole worm - haeCon2 vs. # Ascaris suum/Pig roundworm - ascSuu1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/haeCon2/bed/lastzAscSuu1.2015-08-17 cd /hive/data/genomes/haeCon2/bed/lastzAscSuu1.2015-08-17 cat << '_EOF_' > DEF # haeCon2 vs ascSuu1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Haemonchus contortus/Barber pole worm - haeCon2 SEQ1_DIR=/hive/data/genomes/haeCon2/haeCon2.2bit SEQ1_LEN=/hive/data/genomes/haeCon2/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Ascaris suum/Pig roundworm - ascSuu1 SEQ2_DIR=/hive/data/genomes/ascSuu1/ascSuu1.2bit SEQ2_LEN=/hive/data/genomes/ascSuu1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/haeCon2/bed/lastzAscSuu1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 13m4.773s cat fb.haeCon2.chainAscSuu1Link.txt # 9271571 bases of 346001353 (2.680%) in intersection time (doRecipBest.pl -buildDir=`pwd` haeCon2 ascSuu1) > rbest.log 2>&1 & # real 0m57.452s # and for the swap: mkdir /hive/data/genomes/ascSuu1/bed/blastz.haeCon2.swap cd /hive/data/genomes/ascSuu1/bed/blastz.haeCon2.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/haeCon2/bed/lastzAscSuu1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m11.450s cat fb.ascSuu1.chainHaeCon2Link.txt # 6618939 bases of 262604320 (2.520%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` ascSuu1 haeCon2) \ > rbest.log 2>&1 # real 0m50.406s ######################################################################## ######################################################################## # LASTZ Haemonchus contortus/Barber pole worm - haeCon2 vs. # Brugia malayi/Filarial worm - bruMal2 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/haeCon2/bed/lastzBruMal2.2015-08-17 cd /hive/data/genomes/haeCon2/bed/lastzBruMal2.2015-08-17 cat << '_EOF_' > DEF # haeCon2 vs bruMal2 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Haemonchus contortus/Barber pole worm - haeCon2 SEQ1_DIR=/hive/data/genomes/haeCon2/haeCon2.2bit SEQ1_LEN=/hive/data/genomes/haeCon2/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Brugia malayi/Filarial worm - bruMal2 SEQ2_DIR=/hive/data/genomes/bruMal2/bruMal2.2bit SEQ2_LEN=/hive/data/genomes/bruMal2/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/haeCon2/bed/lastzBruMal2.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 7m57.249s cat fb.haeCon2.chainBruMal2Link.txt # 7436838 bases of 346001353 (2.149%) in intersection time (doRecipBest.pl -buildDir=`pwd` haeCon2 bruMal2) > rbest.log 2>&1 & # real 0m49.488s # and for the swap: mkdir /hive/data/genomes/bruMal2/bed/blastz.haeCon2.swap cd /hive/data/genomes/bruMal2/bed/blastz.haeCon2.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/haeCon2/bed/lastzBruMal2.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m43.629s cat fb.bruMal2.chainHaeCon2Link.txt # 5609507 bases of 87424120 (6.416%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` bruMal2 haeCon2) \ > rbest.log 2>&1 # real 0m41.908s ######################################################################## ######################################################################## # LASTZ Haemonchus contortus/Barber pole worm - haeCon2 vs. # Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/haeCon2/bed/lastzBurXyl1.2015-08-17 cd /hive/data/genomes/haeCon2/bed/lastzBurXyl1.2015-08-17 cat << '_EOF_' > DEF # haeCon2 vs burXyl1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Haemonchus contortus/Barber pole worm - haeCon2 SEQ1_DIR=/hive/data/genomes/haeCon2/haeCon2.2bit SEQ1_LEN=/hive/data/genomes/haeCon2/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 SEQ2_DIR=/hive/data/genomes/burXyl1/burXyl1.2bit SEQ2_LEN=/hive/data/genomes/burXyl1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/haeCon2/bed/lastzBurXyl1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 6m47.932s cat fb.haeCon2.chainBurXyl1Link.txt # 7012653 bases of 346001353 (2.027%) in intersection time (doRecipBest.pl -buildDir=`pwd` haeCon2 burXyl1) > rbest.log 2>&1 & # real 0m47.227s # and for the swap: mkdir /hive/data/genomes/burXyl1/bed/blastz.haeCon2.swap cd /hive/data/genomes/burXyl1/bed/blastz.haeCon2.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/haeCon2/bed/lastzBurXyl1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m53.144s cat fb.burXyl1.chainHaeCon2Link.txt # 4775560 bases of 73100506 (6.533%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` burXyl1 haeCon2) \ > rbest.log 2>&1 # real 0m35.289s ######################################################################## ######################################################################## # LASTZ Haemonchus contortus/Barber pole worm - haeCon2 vs. # Dirofilaria immitis/Dog heartworm - dirImm1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/haeCon2/bed/lastzDirImm1.2015-08-17 cd /hive/data/genomes/haeCon2/bed/lastzDirImm1.2015-08-17 cat << '_EOF_' > DEF # haeCon2 vs dirImm1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Haemonchus contortus/Barber pole worm - haeCon2 SEQ1_DIR=/hive/data/genomes/haeCon2/haeCon2.2bit SEQ1_LEN=/hive/data/genomes/haeCon2/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Dirofilaria immitis/Dog heartworm - dirImm1 SEQ2_DIR=/hive/data/genomes/dirImm1/dirImm1.2bit SEQ2_LEN=/hive/data/genomes/dirImm1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/haeCon2/bed/lastzDirImm1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 8m17.625s cat fb.haeCon2.chainDirImm1Link.txt # 7117359 bases of 346001353 (2.057%) in intersection time (doRecipBest.pl -buildDir=`pwd` haeCon2 dirImm1) > rbest.log 2>&1 & # real 0m46.928s # and for the swap: mkdir /hive/data/genomes/dirImm1/bed/blastz.haeCon2.swap cd /hive/data/genomes/dirImm1/bed/blastz.haeCon2.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/haeCon2/bed/lastzDirImm1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m6.414s cat fb.dirImm1.chainHaeCon2Link.txt # 5078629 bases of 84923352 (5.980%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` dirImm1 haeCon2) \ > rbest.log 2>&1 # real 0m41.903s ######################################################################## ######################################################################## # LASTZ Haemonchus contortus/Barber pole worm - haeCon2 vs. # Loa loa/Eye worm - loaLoa1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/haeCon2/bed/lastzLoaLoa1.2015-08-17 cd /hive/data/genomes/haeCon2/bed/lastzLoaLoa1.2015-08-17 cat << '_EOF_' > DEF # haeCon2 vs loaLoa1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Haemonchus contortus/Barber pole worm - haeCon2 SEQ1_DIR=/hive/data/genomes/haeCon2/haeCon2.2bit SEQ1_LEN=/hive/data/genomes/haeCon2/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Loa loa/Eye worm - loaLoa1 SEQ2_DIR=/hive/data/genomes/loaLoa1/loaLoa1.2bit SEQ2_LEN=/hive/data/genomes/loaLoa1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/haeCon2/bed/lastzLoaLoa1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 6m17.661s cat fb.haeCon2.chainLoaLoa1Link.txt # 7553477 bases of 346001353 (2.183%) in intersection time (doRecipBest.pl -buildDir=`pwd` haeCon2 loaLoa1) > rbest.log 2>&1 & # real 0m49.209s # and for the swap: mkdir /hive/data/genomes/loaLoa1/bed/blastz.haeCon2.swap cd /hive/data/genomes/loaLoa1/bed/blastz.haeCon2.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/haeCon2/bed/lastzLoaLoa1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m56.467s cat fb.loaLoa1.chainHaeCon2Link.txt # 5297325 bases of 87533141 (6.052%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` loaLoa1 haeCon2) \ > rbest.log 2>&1 # real 0m36.583s ######################################################################## ######################################################################## # LASTZ Haemonchus contortus/Barber pole worm - haeCon2 vs. # Meloidogyne hapla/M. hapla - melHap1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/haeCon2/bed/lastzMelHap1.2015-08-17 cd /hive/data/genomes/haeCon2/bed/lastzMelHap1.2015-08-17 cat << '_EOF_' > DEF # haeCon2 vs melHap1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Haemonchus contortus/Barber pole worm - haeCon2 SEQ1_DIR=/hive/data/genomes/haeCon2/haeCon2.2bit SEQ1_LEN=/hive/data/genomes/haeCon2/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Meloidogyne hapla/M. hapla - melHap1 SEQ2_DIR=/hive/data/genomes/melHap1/melHap1.2bit SEQ2_LEN=/hive/data/genomes/melHap1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/haeCon2/bed/lastzMelHap1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 5m32.141s cat fb.haeCon2.chainMelHap1Link.txt # 4343263 bases of 346001353 (1.255%) in intersection time (doRecipBest.pl -buildDir=`pwd` haeCon2 melHap1) > rbest.log 2>&1 & # real 0m32.825s # and for the swap: mkdir /hive/data/genomes/melHap1/bed/blastz.haeCon2.swap cd /hive/data/genomes/melHap1/bed/blastz.haeCon2.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/haeCon2/bed/lastzMelHap1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m48.930s cat fb.melHap1.chainHaeCon2Link.txt # 2921394 bases of 53017507 (5.510%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` melHap1 haeCon2) \ > rbest.log 2>&1 # real 0m25.148s ######################################################################## ######################################################################## # LASTZ Haemonchus contortus/Barber pole worm - haeCon2 vs. # Meloidogyne incognita/M. incognita - melInc2 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/haeCon2/bed/lastzMelInc2.2015-08-17 cd /hive/data/genomes/haeCon2/bed/lastzMelInc2.2015-08-17 cat << '_EOF_' > DEF # haeCon2 vs melInc2 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Haemonchus contortus/Barber pole worm - haeCon2 SEQ1_DIR=/hive/data/genomes/haeCon2/haeCon2.2bit SEQ1_LEN=/hive/data/genomes/haeCon2/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Meloidogyne incognita/M. incognita - melInc2 SEQ2_DIR=/hive/data/genomes/melInc2/melInc2.2bit SEQ2_LEN=/hive/data/genomes/melInc2/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/haeCon2/bed/lastzMelInc2.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 6m2.230s cat fb.haeCon2.chainMelInc2Link.txt # 4585745 bases of 346001353 (1.325%) in intersection time (doRecipBest.pl -buildDir=`pwd` haeCon2 melInc2) > rbest.log 2>&1 & # real 0m35.545s # and for the swap: mkdir /hive/data/genomes/melInc2/bed/blastz.haeCon2.swap cd /hive/data/genomes/melInc2/bed/blastz.haeCon2.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/haeCon2/bed/lastzMelInc2.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m53.912s cat fb.melInc2.chainHaeCon2Link.txt # 4048533 bases of 82114761 (4.930%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` melInc2 haeCon2) \ > rbest.log 2>&1 # real 0m28.820s ######################################################################## ######################################################################## # LASTZ Haemonchus contortus/Barber pole worm - haeCon2 vs. # Onchocerca volvulus/O. volvulus - oncVol1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/haeCon2/bed/lastzOncVol1.2015-08-17 cd /hive/data/genomes/haeCon2/bed/lastzOncVol1.2015-08-17 cat << '_EOF_' > DEF # haeCon2 vs oncVol1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Haemonchus contortus/Barber pole worm - haeCon2 SEQ1_DIR=/hive/data/genomes/haeCon2/haeCon2.2bit SEQ1_LEN=/hive/data/genomes/haeCon2/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Onchocerca volvulus/O. volvulus - oncVol1 SEQ2_DIR=/hive/data/genomes/oncVol1/oncVol1.2bit SEQ2_LEN=/hive/data/genomes/oncVol1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/haeCon2/bed/lastzOncVol1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 7m41.595s cat fb.haeCon2.chainOncVol1Link.txt # 7577293 bases of 346001353 (2.190%) in intersection time (doRecipBest.pl -buildDir=`pwd` haeCon2 oncVol1) > rbest.log 2>&1 & # real 1m7.944s # and for the swap: mkdir /hive/data/genomes/oncVol1/bed/blastz.haeCon2.swap cd /hive/data/genomes/oncVol1/bed/blastz.haeCon2.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/haeCon2/bed/lastzOncVol1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m52.193s cat fb.oncVol1.chainHaeCon2Link.txt # 5418157 bases of 94327777 (5.744%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` oncVol1 haeCon2) \ > rbest.log 2>&1 # real 0m48.474s ######################################################################## ######################################################################## # LASTZ Haemonchus contortus/Barber pole worm - haeCon2 vs. # Pristionchus exspectatus/P. exspectatus - priExs1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/haeCon2/bed/lastzPriExs1.2015-08-17 cd /hive/data/genomes/haeCon2/bed/lastzPriExs1.2015-08-17 cat << '_EOF_' > DEF # haeCon2 vs priExs1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Haemonchus contortus/Barber pole worm - haeCon2 SEQ1_DIR=/hive/data/genomes/haeCon2/haeCon2.2bit SEQ1_LEN=/hive/data/genomes/haeCon2/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Pristionchus exspectatus/P. exspectatus - priExs1 SEQ2_DIR=/hive/data/genomes/priExs1/priExs1.2bit SEQ2_LEN=/hive/data/genomes/priExs1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/haeCon2/bed/lastzPriExs1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 9m27.597s cat fb.haeCon2.chainPriExs1Link.txt # 9709423 bases of 346001353 (2.806%) in intersection time (doRecipBest.pl -buildDir=`pwd` haeCon2 priExs1) > rbest.log 2>&1 & # real 0m57.215s # and for the swap: mkdir /hive/data/genomes/priExs1/bed/blastz.haeCon2.swap cd /hive/data/genomes/priExs1/bed/blastz.haeCon2.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/haeCon2/bed/lastzPriExs1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m10.139s cat fb.priExs1.chainHaeCon2Link.txt # 7738612 bases of 166917800 (4.636%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` priExs1 haeCon2) \ > rbest.log 2>&1 # real 0m49.063s ######################################################################## ######################################################################## # LASTZ Haemonchus contortus/Barber pole worm - haeCon2 vs. # Pristionchus pacificus/P. pacificus - priPac3 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/haeCon2/bed/lastzPriPac3.2015-08-17 cd /hive/data/genomes/haeCon2/bed/lastzPriPac3.2015-08-17 cat << '_EOF_' > DEF # haeCon2 vs priPac3 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Haemonchus contortus/Barber pole worm - haeCon2 SEQ1_DIR=/hive/data/genomes/haeCon2/haeCon2.2bit SEQ1_LEN=/hive/data/genomes/haeCon2/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Pristionchus pacificus/P. pacificus - priPac3 SEQ2_DIR=/hive/data/genomes/priPac3/priPac3.2bit SEQ2_LEN=/hive/data/genomes/priPac3/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/haeCon2/bed/lastzPriPac3.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 11m19.547s cat fb.haeCon2.chainPriPac3Link.txt # 9845456 bases of 346001353 (2.845%) in intersection time (doRecipBest.pl -buildDir=`pwd` haeCon2 priPac3) > rbest.log 2>&1 & # real 0m58.661s # and for the swap: mkdir /hive/data/genomes/priPac3/bed/blastz.haeCon2.swap cd /hive/data/genomes/priPac3/bed/blastz.haeCon2.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/haeCon2/bed/lastzPriPac3.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m18.815s cat fb.priPac3.chainHaeCon2Link.txt # 7584928 bases of 153238914 (4.950%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` priPac3 haeCon2) \ > rbest.log 2>&1 # real 0m49.836s ######################################################################## ######################################################################## # LASTZ Haemonchus contortus/Barber pole worm - haeCon2 vs. # Trichinella spiralis/Trichinella - triSpi1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/haeCon2/bed/lastzTriSpi1.2015-08-17 cd /hive/data/genomes/haeCon2/bed/lastzTriSpi1.2015-08-17 cat << '_EOF_' > DEF # haeCon2 vs triSpi1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Haemonchus contortus/Barber pole worm - haeCon2 SEQ1_DIR=/hive/data/genomes/haeCon2/haeCon2.2bit SEQ1_LEN=/hive/data/genomes/haeCon2/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Trichinella spiralis/Trichinella - triSpi1 SEQ2_DIR=/hive/data/genomes/triSpi1/triSpi1.2bit SEQ2_LEN=/hive/data/genomes/triSpi1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/haeCon2/bed/lastzTriSpi1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 6m23.356s cat fb.haeCon2.chainTriSpi1Link.txt # 3521590 bases of 346001353 (1.018%) in intersection time (doRecipBest.pl -buildDir=`pwd` haeCon2 triSpi1) > rbest.log 2>&1 & # real 0m35.108s # and for the swap: mkdir /hive/data/genomes/triSpi1/bed/blastz.haeCon2.swap cd /hive/data/genomes/triSpi1/bed/blastz.haeCon2.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/haeCon2/bed/lastzTriSpi1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m47.288s cat fb.triSpi1.chainHaeCon2Link.txt # 2583045 bases of 58558192 (4.411%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` triSpi1 haeCon2) \ > rbest.log 2>&1 # real 0m24.742s ######################################################################## ######################################################################## # LASTZ Haemonchus contortus/Barber pole worm - haeCon2 vs. # Trichuris suis/Whipworm - triSui1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/haeCon2/bed/lastzTriSui1.2015-08-17 cd /hive/data/genomes/haeCon2/bed/lastzTriSui1.2015-08-17 cat << '_EOF_' > DEF # haeCon2 vs triSui1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Haemonchus contortus/Barber pole worm - haeCon2 SEQ1_DIR=/hive/data/genomes/haeCon2/haeCon2.2bit SEQ1_LEN=/hive/data/genomes/haeCon2/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Trichuris suis/Whipworm - triSui1 SEQ2_DIR=/hive/data/genomes/triSui1/triSui1.2bit SEQ2_LEN=/hive/data/genomes/triSui1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/haeCon2/bed/lastzTriSui1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 10m50.341s cat fb.haeCon2.chainTriSui1Link.txt # 4007129 bases of 346001353 (1.158%) in intersection time (doRecipBest.pl -buildDir=`pwd` haeCon2 triSui1) > rbest.log 2>&1 & # real 0m41.532s # and for the swap: mkdir /hive/data/genomes/triSui1/bed/blastz.haeCon2.swap cd /hive/data/genomes/triSui1/bed/blastz.haeCon2.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/haeCon2/bed/lastzTriSui1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 2m9.786s cat fb.triSui1.chainHaeCon2Link.txt # 2979584 bases of 71825323 (4.148%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` triSui1 haeCon2) \ > rbest.log 2>&1 # real 0m23.298s ########################################################################