######################################################################## # LASTZ Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 vs. # Ancylostoma ceylanicum/A. ceylanicum - ancCey1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/caeSp51/bed/lastzAncCey1.2015-08-16 cd /hive/data/genomes/caeSp51/bed/lastzAncCey1.2015-08-16 cat << '_EOF_' > DEF # caeSp51 vs ancCey1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 SEQ1_DIR=/hive/data/genomes/caeSp51/caeSp51.2bit SEQ1_LEN=/hive/data/genomes/caeSp51/chrom.sizes SEQ1_CHUNK=1000000 SEQ1_LAP=10000 # QUERY: Ancylostoma ceylanicum/A. ceylanicum - ancCey1 SEQ2_DIR=/hive/data/genomes/ancCey1/ancCey1.2bit SEQ2_LEN=/hive/data/genomes/ancCey1/chrom.sizes SEQ2_CHUNK=1000000 SEQ2_LIMIT=50 SEQ2_LAP=0 BASE=/hive/data/genomes/caeSp51/bed/lastzAncCey1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 39m50.842s cat fb.caeSp51.chainAncCey1Link.txt # 9543742 bases of 130562982 (7.310%) in intersection time (doRecipBest.pl -buildDir=`pwd` caeSp51 ancCey1) > rbest.log 2>&1 & # real 0m50.610s # and for the swap: mkdir /hive/data/genomes/ancCey1/bed/blastz.caeSp51.swap cd /hive/data/genomes/ancCey1/bed/blastz.caeSp51.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/caeSp51/bed/lastzAncCey1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m8.943s cat fb.ancCey1.chainCaeSp51Link.txt # 10520214 bases of 300914990 (3.496%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` ancCey1 caeSp51) \ > rbest.log 2>&1 # real 0m50.531s ######################################################################## ######################################################################## # LASTZ Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 vs. # Ascaris suum/Pig roundworm - ascSuu1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/caeSp51/bed/lastzAscSuu1.2015-08-16 cd /hive/data/genomes/caeSp51/bed/lastzAscSuu1.2015-08-16 cat << '_EOF_' > DEF # caeSp51 vs ascSuu1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 SEQ1_DIR=/hive/data/genomes/caeSp51/caeSp51.2bit SEQ1_LEN=/hive/data/genomes/caeSp51/chrom.sizes SEQ1_CHUNK=1000000 SEQ1_LAP=10000 # QUERY: Ascaris suum/Pig roundworm - ascSuu1 SEQ2_DIR=/hive/data/genomes/ascSuu1/ascSuu1.2bit SEQ2_LEN=/hive/data/genomes/ascSuu1/chrom.sizes SEQ2_CHUNK=1000000 SEQ2_LIMIT=50 SEQ2_LAP=0 BASE=/hive/data/genomes/caeSp51/bed/lastzAscSuu1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 49m55.949s cat fb.caeSp51.chainAscSuu1Link.txt # 5206961 bases of 130562982 (3.988%) in intersection time (doRecipBest.pl -buildDir=`pwd` caeSp51 ascSuu1) > rbest.log 2>&1 & # real 0m40.029s # and for the swap: mkdir /hive/data/genomes/ascSuu1/bed/blastz.caeSp51.swap cd /hive/data/genomes/ascSuu1/bed/blastz.caeSp51.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/caeSp51/bed/lastzAscSuu1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 3m7.229s cat fb.ascSuu1.chainCaeSp51Link.txt # 5214455 bases of 262604320 (1.986%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` ascSuu1 caeSp51) \ > rbest.log 2>&1 # real 3m2.722s ######################################################################## ######################################################################## # LASTZ Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 vs. # Brugia malayi/Filarial worm - bruMal2 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/caeSp51/bed/lastzBruMal2.2015-08-16 cd /hive/data/genomes/caeSp51/bed/lastzBruMal2.2015-08-16 cat << '_EOF_' > DEF # caeSp51 vs bruMal2 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 SEQ1_DIR=/hive/data/genomes/caeSp51/caeSp51.2bit SEQ1_LEN=/hive/data/genomes/caeSp51/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Brugia malayi/Filarial worm - bruMal2 SEQ2_DIR=/hive/data/genomes/bruMal2/bruMal2.2bit SEQ2_LEN=/hive/data/genomes/bruMal2/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/caeSp51/bed/lastzBruMal2.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 5m39.466s cat fb.caeSp51.chainBruMal2Link.txt # 4309251 bases of 130562982 (3.301%) in intersection time (doRecipBest.pl -buildDir=`pwd` caeSp51 bruMal2) > rbest.log 2>&1 & # real 0m33.858s # and for the swap: mkdir /hive/data/genomes/bruMal2/bed/blastz.caeSp51.swap cd /hive/data/genomes/bruMal2/bed/blastz.caeSp51.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/caeSp51/bed/lastzBruMal2.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m25.087s cat fb.bruMal2.chainCaeSp51Link.txt # 4094986 bases of 87424120 (4.684%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` bruMal2 caeSp51) \ > rbest.log 2>&1 # real 0m30.822s ######################################################################## ######################################################################## # LASTZ Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 vs. # Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/caeSp51/bed/lastzBurXyl1.2015-08-16 cd /hive/data/genomes/caeSp51/bed/lastzBurXyl1.2015-08-16 cat << '_EOF_' > DEF # caeSp51 vs burXyl1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 SEQ1_DIR=/hive/data/genomes/caeSp51/caeSp51.2bit SEQ1_LEN=/hive/data/genomes/caeSp51/chrom.sizes SEQ1_CHUNK=1000000 SEQ1_LAP=10000 # QUERY: Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 SEQ2_DIR=/hive/data/genomes/burXyl1/burXyl1.2bit SEQ2_LEN=/hive/data/genomes/burXyl1/chrom.sizes SEQ2_CHUNK=1000000 SEQ2_LIMIT=50 SEQ2_LAP=0 BASE=/hive/data/genomes/caeSp51/bed/lastzBurXyl1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 23m24.862s cat fb.caeSp51.chainBurXyl1Link.txt # 6181055 bases of 130562982 (4.734%) in intersection time (doRecipBest.pl -buildDir=`pwd` caeSp51 burXyl1) > rbest.log 2>&1 & # real 0m37.631s # and for the swap: mkdir /hive/data/genomes/burXyl1/bed/blastz.caeSp51.swap cd /hive/data/genomes/burXyl1/bed/blastz.caeSp51.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/caeSp51/bed/lastzBurXyl1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m53.043s cat fb.burXyl1.chainCaeSp51Link.txt # 5795009 bases of 73100506 (7.927%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` burXyl1 caeSp51) \ > rbest.log 2>&1 # real 0m35.510s ######################################################################## ######################################################################## # LASTZ Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 vs. # Dirofilaria immitis/Dog heartworm - dirImm1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/caeSp51/bed/lastzDirImm1.2015-08-16 cd /hive/data/genomes/caeSp51/bed/lastzDirImm1.2015-08-16 cat << '_EOF_' > DEF # caeSp51 vs dirImm1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 SEQ1_DIR=/hive/data/genomes/caeSp51/caeSp51.2bit SEQ1_LEN=/hive/data/genomes/caeSp51/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Dirofilaria immitis/Dog heartworm - dirImm1 SEQ2_DIR=/hive/data/genomes/dirImm1/dirImm1.2bit SEQ2_LEN=/hive/data/genomes/dirImm1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/caeSp51/bed/lastzDirImm1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 8m6.569s cat fb.caeSp51.chainDirImm1Link.txt # 4221631 bases of 130562982 (3.233%) in intersection time (doRecipBest.pl -buildDir=`pwd` caeSp51 dirImm1) > rbest.log 2>&1 & # real 0m34.429s # and for the swap: mkdir /hive/data/genomes/dirImm1/bed/blastz.caeSp51.swap cd /hive/data/genomes/dirImm1/bed/blastz.caeSp51.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/caeSp51/bed/lastzDirImm1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m51.934s cat fb.dirImm1.chainCaeSp51Link.txt # 3881592 bases of 84923352 (4.571%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` dirImm1 caeSp51) \ > rbest.log 2>&1 # real 0m44.703s ######################################################################## ######################################################################## # LASTZ Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 vs. # Haemonchus contortus/Barber pole worm - haeCon2 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/caeSp51/bed/lastzHaeCon2.2015-08-16 cd /hive/data/genomes/caeSp51/bed/lastzHaeCon2.2015-08-16 cat << '_EOF_' > DEF # caeSp51 vs haeCon2 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 SEQ1_DIR=/hive/data/genomes/caeSp51/caeSp51.2bit SEQ1_LEN=/hive/data/genomes/caeSp51/chrom.sizes SEQ1_CHUNK=1000000 SEQ1_LAP=10000 # QUERY: Haemonchus contortus/Barber pole worm - haeCon2 SEQ2_DIR=/hive/data/genomes/haeCon2/haeCon2.2bit SEQ2_LEN=/hive/data/genomes/haeCon2/chrom.sizes SEQ2_CHUNK=1000000 SEQ2_LIMIT=50 SEQ2_LAP=0 BASE=/hive/data/genomes/caeSp51/bed/lastzHaeCon2.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 59m43.690s cat fb.caeSp51.chainHaeCon2Link.txt # 8561473 bases of 130562982 (6.557%) in intersection time (doRecipBest.pl -buildDir=`pwd` caeSp51 haeCon2) > rbest.log 2>&1 & # real 0m53.397s # and for the swap: mkdir /hive/data/genomes/haeCon2/bed/blastz.caeSp51.swap cd /hive/data/genomes/haeCon2/bed/blastz.caeSp51.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/caeSp51/bed/lastzHaeCon2.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 3m3.530s cat fb.haeCon2.chainCaeSp51Link.txt # 12629971 bases of 346001353 (3.650%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` haeCon2 caeSp51) \ > rbest.log 2>&1 # real 5m26.262s ######################################################################## ######################################################################## # LASTZ Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 vs. # Heterorhabditis bacteriophora/H. bacteriophora/m31e - hetBac1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/caeSp51/bed/lastzHetBac1.2015-08-16 cd /hive/data/genomes/caeSp51/bed/lastzHetBac1.2015-08-16 cat << '_EOF_' > DEF # caeSp51 vs hetBac1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 SEQ1_DIR=/hive/data/genomes/caeSp51/caeSp51.2bit SEQ1_LEN=/hive/data/genomes/caeSp51/chrom.sizes SEQ1_CHUNK=1000000 SEQ1_LAP=10000 # QUERY: Heterorhabditis bacteriophora/H. bacteriophora/m31e - hetBac1 SEQ2_DIR=/hive/data/genomes/hetBac1/hetBac1.2bit SEQ2_LEN=/hive/data/genomes/hetBac1/chrom.sizes SEQ2_CHUNK=1000000 SEQ2_LIMIT=50 SEQ2_LAP=0 BASE=/hive/data/genomes/caeSp51/bed/lastzHetBac1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 16m44.509s cat fb.caeSp51.chainHetBac1Link.txt # 9770271 bases of 130562982 (7.483%) in intersection time (doRecipBest.pl -buildDir=`pwd` caeSp51 hetBac1) > rbest.log 2>&1 & # real 0m45.627s # and for the swap: mkdir /hive/data/genomes/hetBac1/bed/blastz.caeSp51.swap cd /hive/data/genomes/hetBac1/bed/blastz.caeSp51.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/caeSp51/bed/lastzHetBac1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m1.960s cat fb.hetBac1.chainCaeSp51Link.txt # 9504814 bases of 74374900 (12.780%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` hetBac1 caeSp51) \ > rbest.log 2>&1 # real 0m43.520s ######################################################################## ######################################################################## # LASTZ Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 vs. # Loa loa/Eye worm - loaLoa1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/caeSp51/bed/lastzLoaLoa1.2015-08-16 cd /hive/data/genomes/caeSp51/bed/lastzLoaLoa1.2015-08-16 cat << '_EOF_' > DEF # caeSp51 vs loaLoa1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 SEQ1_DIR=/hive/data/genomes/caeSp51/caeSp51.2bit SEQ1_LEN=/hive/data/genomes/caeSp51/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Loa loa/Eye worm - loaLoa1 SEQ2_DIR=/hive/data/genomes/loaLoa1/loaLoa1.2bit SEQ2_LEN=/hive/data/genomes/loaLoa1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/caeSp51/bed/lastzLoaLoa1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 5m52.876s cat fb.caeSp51.chainLoaLoa1Link.txt # 4381020 bases of 130562982 (3.355%) in intersection time (doRecipBest.pl -buildDir=`pwd` caeSp51 loaLoa1) > rbest.log 2>&1 & # real 0m32.059s # and for the swap: mkdir /hive/data/genomes/loaLoa1/bed/blastz.caeSp51.swap cd /hive/data/genomes/loaLoa1/bed/blastz.caeSp51.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/caeSp51/bed/lastzLoaLoa1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m49.006s cat fb.loaLoa1.chainCaeSp51Link.txt # 3980536 bases of 87533141 (4.547%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` loaLoa1 caeSp51) \ > rbest.log 2>&1 # real 0m29.661s ######################################################################## ######################################################################## # LASTZ Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 vs. # Meloidogyne hapla/M. hapla - melHap1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/caeSp51/bed/lastzMelHap1.2015-08-16 cd /hive/data/genomes/caeSp51/bed/lastzMelHap1.2015-08-16 cat << '_EOF_' > DEF # caeSp51 vs melHap1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 SEQ1_DIR=/hive/data/genomes/caeSp51/caeSp51.2bit SEQ1_LEN=/hive/data/genomes/caeSp51/chrom.sizes SEQ1_CHUNK=1000000 SEQ1_LAP=10000 # QUERY: Meloidogyne hapla/M. hapla - melHap1 SEQ2_DIR=/hive/data/genomes/melHap1/melHap1.2bit SEQ2_LEN=/hive/data/genomes/melHap1/chrom.sizes SEQ2_CHUNK=1000000 SEQ2_LIMIT=50 SEQ2_LAP=0 BASE=/hive/data/genomes/caeSp51/bed/lastzMelHap1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 15m24.656s cat fb.caeSp51.chainMelHap1Link.txt # 3371583 bases of 130562982 (2.582%) in intersection time (doRecipBest.pl -buildDir=`pwd` caeSp51 melHap1) > rbest.log 2>&1 & # real 0m27.952s # and for the swap: mkdir /hive/data/genomes/melHap1/bed/blastz.caeSp51.swap cd /hive/data/genomes/melHap1/bed/blastz.caeSp51.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/caeSp51/bed/lastzMelHap1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m46.696s cat fb.melHap1.chainCaeSp51Link.txt # 3180220 bases of 53017507 (5.998%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` melHap1 caeSp51) \ > rbest.log 2>&1 # real 0m24.101s ######################################################################## ######################################################################## # LASTZ Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 vs. # Meloidogyne incognita/M. incognita - melInc2 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/caeSp51/bed/lastzMelInc2.2015-08-16 cd /hive/data/genomes/caeSp51/bed/lastzMelInc2.2015-08-16 cat << '_EOF_' > DEF # caeSp51 vs melInc2 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 SEQ1_DIR=/hive/data/genomes/caeSp51/caeSp51.2bit SEQ1_LEN=/hive/data/genomes/caeSp51/chrom.sizes SEQ1_CHUNK=1000000 SEQ1_LAP=10000 # QUERY: Meloidogyne incognita/M. incognita - melInc2 SEQ2_DIR=/hive/data/genomes/melInc2/melInc2.2bit SEQ2_LEN=/hive/data/genomes/melInc2/chrom.sizes SEQ2_CHUNK=1000000 SEQ2_LIMIT=50 SEQ2_LAP=0 BASE=/hive/data/genomes/caeSp51/bed/lastzMelInc2.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 16m34.480s cat fb.caeSp51.chainMelInc2Link.txt # 3115114 bases of 130562982 (2.386%) in intersection time (doRecipBest.pl -buildDir=`pwd` caeSp51 melInc2) > rbest.log 2>&1 & # real 0m31.601s # and for the swap: mkdir /hive/data/genomes/melInc2/bed/blastz.caeSp51.swap cd /hive/data/genomes/melInc2/bed/blastz.caeSp51.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/caeSp51/bed/lastzMelInc2.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 3m45.116s cat fb.melInc2.chainCaeSp51Link.txt # 4178328 bases of 82114761 (5.088%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` melInc2 caeSp51) \ > rbest.log 2>&1 # real 0m26.122s ######################################################################## ######################################################################## # LASTZ Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 vs. # Necator americanus/N. americanus - necAme1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/caeSp51/bed/lastzNecAme1.2015-08-16 cd /hive/data/genomes/caeSp51/bed/lastzNecAme1.2015-08-16 cat << '_EOF_' > DEF # caeSp51 vs necAme1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 SEQ1_DIR=/hive/data/genomes/caeSp51/caeSp51.2bit SEQ1_LEN=/hive/data/genomes/caeSp51/chrom.sizes SEQ1_CHUNK=1000000 SEQ1_LAP=10000 # QUERY: Necator americanus/N. americanus - necAme1 SEQ2_DIR=/hive/data/genomes/necAme1/necAme1.2bit SEQ2_LEN=/hive/data/genomes/necAme1/chrom.sizes SEQ2_CHUNK=1000000 SEQ2_LIMIT=50 SEQ2_LAP=0 BASE=/hive/data/genomes/caeSp51/bed/lastzNecAme1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 38m13.808s cat fb.caeSp51.chainNecAme1Link.txt # 8442567 bases of 130562982 (6.466%) in intersection time (doRecipBest.pl -buildDir=`pwd` caeSp51 necAme1) > rbest.log 2>&1 & # real 0m50.563s # and for the swap: mkdir /hive/data/genomes/necAme1/bed/blastz.caeSp51.swap cd /hive/data/genomes/necAme1/bed/blastz.caeSp51.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/caeSp51/bed/lastzNecAme1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m14.437s cat fb.necAme1.chainCaeSp51Link.txt # 8430633 bases of 208187215 (4.050%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` necAme1 caeSp51) \ > rbest.log 2>&1 # real 0m53.392s ######################################################################## ######################################################################## # LASTZ Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 vs. # Onchocerca volvulus/O. volvulus - oncVol1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/caeSp51/bed/lastzOncVol1.2015-08-16 cd /hive/data/genomes/caeSp51/bed/lastzOncVol1.2015-08-16 cat << '_EOF_' > DEF # caeSp51 vs oncVol1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 SEQ1_DIR=/hive/data/genomes/caeSp51/caeSp51.2bit SEQ1_LEN=/hive/data/genomes/caeSp51/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Onchocerca volvulus/O. volvulus - oncVol1 SEQ2_DIR=/hive/data/genomes/oncVol1/oncVol1.2bit SEQ2_LEN=/hive/data/genomes/oncVol1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/caeSp51/bed/lastzOncVol1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 5m48.638s cat fb.caeSp51.chainOncVol1Link.txt # 4443511 bases of 130562982 (3.403%) in intersection time (doRecipBest.pl -buildDir=`pwd` caeSp51 oncVol1) > rbest.log 2>&1 & # real 1m4.839s # and for the swap: mkdir /hive/data/genomes/oncVol1/bed/blastz.caeSp51.swap cd /hive/data/genomes/oncVol1/bed/blastz.caeSp51.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/caeSp51/bed/lastzOncVol1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m45.141s cat fb.oncVol1.chainCaeSp51Link.txt # 4114552 bases of 94327777 (4.362%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` oncVol1 caeSp51) \ > rbest.log 2>&1 # real 0m37.430s ######################################################################## ######################################################################## # LASTZ Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 vs. # Panagrellus redivivus/Microworm - panRed1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/caeSp51/bed/lastzPanRed1.2015-08-16 cd /hive/data/genomes/caeSp51/bed/lastzPanRed1.2015-08-16 cat << '_EOF_' > DEF # caeSp51 vs panRed1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 SEQ1_DIR=/hive/data/genomes/caeSp51/caeSp51.2bit SEQ1_LEN=/hive/data/genomes/caeSp51/chrom.sizes SEQ1_CHUNK=1000000 SEQ1_LAP=10000 # QUERY: Panagrellus redivivus/Microworm - panRed1 SEQ2_DIR=/hive/data/genomes/panRed1/panRed1.2bit SEQ2_LEN=/hive/data/genomes/panRed1/chrom.sizes SEQ2_CHUNK=1000000 SEQ2_LIMIT=50 SEQ2_LAP=0 BASE=/hive/data/genomes/caeSp51/bed/lastzPanRed1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 15m11.779s cat fb.caeSp51.chainPanRed1Link.txt # 6644023 bases of 130562982 (5.089%) in intersection time (doRecipBest.pl -buildDir=`pwd` caeSp51 panRed1) > rbest.log 2>&1 & # real 0m37.332s # and for the swap: mkdir /hive/data/genomes/panRed1/bed/blastz.caeSp51.swap cd /hive/data/genomes/panRed1/bed/blastz.caeSp51.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/caeSp51/bed/lastzPanRed1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m51.599s cat fb.panRed1.chainCaeSp51Link.txt # 6349865 bases of 62228262 (10.204%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` panRed1 caeSp51) \ > rbest.log 2>&1 # real 0m34.522s ######################################################################## ######################################################################## # LASTZ Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 vs. # Pristionchus exspectatus/P. exspectatus - priExs1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/caeSp51/bed/lastzPriExs1.2015-08-16 cd /hive/data/genomes/caeSp51/bed/lastzPriExs1.2015-08-16 cat << '_EOF_' > DEF # caeSp51 vs priExs1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 SEQ1_DIR=/hive/data/genomes/caeSp51/caeSp51.2bit SEQ1_LEN=/hive/data/genomes/caeSp51/chrom.sizes SEQ1_CHUNK=1000000 SEQ1_LAP=10000 # QUERY: Pristionchus exspectatus/P. exspectatus - priExs1 SEQ2_DIR=/hive/data/genomes/priExs1/priExs1.2bit SEQ2_LEN=/hive/data/genomes/priExs1/chrom.sizes SEQ2_CHUNK=1000000 SEQ2_LIMIT=50 SEQ2_LAP=0 BASE=/hive/data/genomes/caeSp51/bed/lastzPriExs1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 33m14.977s cat fb.caeSp51.chainPriExs1Link.txt # 6225920 bases of 130562982 (4.769%) in intersection time (doRecipBest.pl -buildDir=`pwd` caeSp51 priExs1) > rbest.log 2>&1 & # real 0m41.454s # and for the swap: mkdir /hive/data/genomes/priExs1/bed/blastz.caeSp51.swap cd /hive/data/genomes/priExs1/bed/blastz.caeSp51.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/caeSp51/bed/lastzPriExs1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 3m56.571s cat fb.priExs1.chainCaeSp51Link.txt # 7147403 bases of 166917800 (4.282%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` priExs1 caeSp51) \ > rbest.log 2>&1 # real 3m27.367s ######################################################################## ######################################################################## # LASTZ Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 vs. # Pristionchus pacificus/P. pacificus - priPac3 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/caeSp51/bed/lastzPriPac3.2015-08-16 cd /hive/data/genomes/caeSp51/bed/lastzPriPac3.2015-08-16 cat << '_EOF_' > DEF # caeSp51 vs priPac3 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 SEQ1_DIR=/hive/data/genomes/caeSp51/caeSp51.2bit SEQ1_LEN=/hive/data/genomes/caeSp51/chrom.sizes SEQ1_CHUNK=1000000 SEQ1_LAP=10000 # QUERY: Pristionchus pacificus/P. pacificus - priPac3 SEQ2_DIR=/hive/data/genomes/priPac3/priPac3.2bit SEQ2_LEN=/hive/data/genomes/priPac3/chrom.sizes SEQ2_CHUNK=1000000 SEQ2_LIMIT=50 SEQ2_LAP=0 BASE=/hive/data/genomes/caeSp51/bed/lastzPriPac3.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 44m45.685s cat fb.caeSp51.chainPriPac3Link.txt # 6254484 bases of 130562982 (4.790%) in intersection time (doRecipBest.pl -buildDir=`pwd` caeSp51 priPac3) > rbest.log 2>&1 & # real 0m43.334s # and for the swap: mkdir /hive/data/genomes/priPac3/bed/blastz.caeSp51.swap cd /hive/data/genomes/priPac3/bed/blastz.caeSp51.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/caeSp51/bed/lastzPriPac3.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m9.491s cat fb.priPac3.chainCaeSp51Link.txt # 6880001 bases of 153238914 (4.490%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` priPac3 caeSp51) \ > rbest.log 2>&1 # real 0m44.772s ######################################################################## ######################################################################## # LASTZ Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 vs. # Strongyloides ratti/Threadworm - strRat2 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/caeSp51/bed/lastzStrRat2.2015-08-16 cd /hive/data/genomes/caeSp51/bed/lastzStrRat2.2015-08-16 cat << '_EOF_' > DEF # caeSp51 vs strRat2 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 SEQ1_DIR=/hive/data/genomes/caeSp51/caeSp51.2bit SEQ1_LEN=/hive/data/genomes/caeSp51/chrom.sizes SEQ1_CHUNK=1000000 SEQ1_LAP=10000 # QUERY: Strongyloides ratti/Threadworm - strRat2 SEQ2_DIR=/hive/data/genomes/strRat2/strRat2.2bit SEQ2_LEN=/hive/data/genomes/strRat2/chrom.sizes SEQ2_CHUNK=1000000 SEQ2_LIMIT=50 SEQ2_LAP=0 BASE=/hive/data/genomes/caeSp51/bed/lastzStrRat2.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 9m19.896s cat fb.caeSp51.chainStrRat2Link.txt # 4926675 bases of 130562982 (3.773%) in intersection time (doRecipBest.pl -buildDir=`pwd` caeSp51 strRat2) > rbest.log 2>&1 & # real 0m36.340s # and for the swap: mkdir /hive/data/genomes/strRat2/bed/blastz.caeSp51.swap cd /hive/data/genomes/strRat2/bed/blastz.caeSp51.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/caeSp51/bed/lastzStrRat2.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m44.126s cat fb.strRat2.chainCaeSp51Link.txt # 4410021 bases of 43150242 (10.220%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` strRat2 caeSp51) \ > rbest.log 2>&1 # real 0m29.907s ######################################################################## ######################################################################## # LASTZ Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 vs. # Trichinella spiralis/Trichinella - triSpi1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/caeSp51/bed/lastzTriSpi1.2015-08-16 cd /hive/data/genomes/caeSp51/bed/lastzTriSpi1.2015-08-16 cat << '_EOF_' > DEF # caeSp51 vs triSpi1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 SEQ1_DIR=/hive/data/genomes/caeSp51/caeSp51.2bit SEQ1_LEN=/hive/data/genomes/caeSp51/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Trichinella spiralis/Trichinella - triSpi1 SEQ2_DIR=/hive/data/genomes/triSpi1/triSpi1.2bit SEQ2_LEN=/hive/data/genomes/triSpi1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/caeSp51/bed/lastzTriSpi1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 5m30.011s cat fb.caeSp51.chainTriSpi1Link.txt # 2610513 bases of 130562982 (1.999%) in intersection time (doRecipBest.pl -buildDir=`pwd` caeSp51 triSpi1) > rbest.log 2>&1 & # real 0m28.548s # and for the swap: mkdir /hive/data/genomes/triSpi1/bed/blastz.caeSp51.swap cd /hive/data/genomes/triSpi1/bed/blastz.caeSp51.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/caeSp51/bed/lastzTriSpi1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m44.766s cat fb.triSpi1.chainCaeSp51Link.txt # 2634022 bases of 58558192 (4.498%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` triSpi1 caeSp51) \ > rbest.log 2>&1 # real 0m22.162s ######################################################################## ######################################################################## # LASTZ Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 vs. # Trichuris suis/Whipworm - triSui1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/caeSp51/bed/lastzTriSui1.2015-08-16 cd /hive/data/genomes/caeSp51/bed/lastzTriSui1.2015-08-16 cat << '_EOF_' > DEF # caeSp51 vs triSui1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Caenorhabditis sp5 ju800/C. sp. 5 ju800 - caeSp51 SEQ1_DIR=/hive/data/genomes/caeSp51/caeSp51.2bit SEQ1_LEN=/hive/data/genomes/caeSp51/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Trichuris suis/Whipworm - triSui1 SEQ2_DIR=/hive/data/genomes/triSui1/triSui1.2bit SEQ2_LEN=/hive/data/genomes/triSui1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/caeSp51/bed/lastzTriSui1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 5m1.254s cat fb.caeSp51.chainTriSui1Link.txt # 2841785 bases of 130562982 (2.177%) in intersection time (doRecipBest.pl -buildDir=`pwd` caeSp51 triSui1) > rbest.log 2>&1 & # real 0m25.640s # and for the swap: mkdir /hive/data/genomes/triSui1/bed/blastz.caeSp51.swap cd /hive/data/genomes/triSui1/bed/blastz.caeSp51.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/caeSp51/bed/lastzTriSui1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m42.991s cat fb.triSui1.chainCaeSp51Link.txt # 2717654 bases of 71825323 (3.784%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` triSui1 caeSp51) \ > rbest.log 2>&1 # real 0m21.988s ########################################################################