######################################################################## # LASTZ Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 vs. # Brugia malayi/Filarial worm - bruMal2 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/burXyl1/bed/lastzBruMal2.2015-08-17 cd /hive/data/genomes/burXyl1/bed/lastzBruMal2.2015-08-17 cat << '_EOF_' > DEF # burXyl1 vs bruMal2 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 SEQ1_DIR=/hive/data/genomes/burXyl1/burXyl1.2bit SEQ1_LEN=/hive/data/genomes/burXyl1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Brugia malayi/Filarial worm - bruMal2 SEQ2_DIR=/hive/data/genomes/bruMal2/bruMal2.2bit SEQ2_LEN=/hive/data/genomes/bruMal2/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/burXyl1/bed/lastzBruMal2.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 3m38.873s cat fb.burXyl1.chainBruMal2Link.txt # 4276207 bases of 73100506 (5.850%) in intersection time (doRecipBest.pl -buildDir=`pwd` burXyl1 bruMal2) > rbest.log 2>&1 & # real 10m27.476s # and for the swap: mkdir /hive/data/genomes/bruMal2/bed/blastz.burXyl1.swap cd /hive/data/genomes/bruMal2/bed/blastz.burXyl1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/burXyl1/bed/lastzBruMal2.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m29.297s cat fb.bruMal2.chainBurXyl1Link.txt # 4352556 bases of 87424120 (4.979%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` bruMal2 burXyl1) \ > rbest.log 2>&1 # real 0m32.805s ######################################################################## ######################################################################## # LASTZ Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 vs. # Dirofilaria immitis/Dog heartworm - dirImm1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/burXyl1/bed/lastzDirImm1.2015-08-17 cd /hive/data/genomes/burXyl1/bed/lastzDirImm1.2015-08-17 cat << '_EOF_' > DEF # burXyl1 vs dirImm1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 SEQ1_DIR=/hive/data/genomes/burXyl1/burXyl1.2bit SEQ1_LEN=/hive/data/genomes/burXyl1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Dirofilaria immitis/Dog heartworm - dirImm1 SEQ2_DIR=/hive/data/genomes/dirImm1/dirImm1.2bit SEQ2_LEN=/hive/data/genomes/dirImm1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/burXyl1/bed/lastzDirImm1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 3m47.024s cat fb.burXyl1.chainDirImm1Link.txt # 4172810 bases of 73100506 (5.708%) in intersection time (doRecipBest.pl -buildDir=`pwd` burXyl1 dirImm1) > rbest.log 2>&1 & # real 4m24.670s # and for the swap: mkdir /hive/data/genomes/dirImm1/bed/blastz.burXyl1.swap cd /hive/data/genomes/dirImm1/bed/blastz.burXyl1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/burXyl1/bed/lastzDirImm1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m2.076s cat fb.dirImm1.chainBurXyl1Link.txt # 4086607 bases of 84923352 (4.812%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` dirImm1 burXyl1) \ > rbest.log 2>&1 # real 0m36.222s ######################################################################## ######################################################################## # LASTZ Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 vs. # Loa loa/Eye worm - loaLoa1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/burXyl1/bed/lastzLoaLoa1.2015-08-17 cd /hive/data/genomes/burXyl1/bed/lastzLoaLoa1.2015-08-17 cat << '_EOF_' > DEF # burXyl1 vs loaLoa1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 SEQ1_DIR=/hive/data/genomes/burXyl1/burXyl1.2bit SEQ1_LEN=/hive/data/genomes/burXyl1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Loa loa/Eye worm - loaLoa1 SEQ2_DIR=/hive/data/genomes/loaLoa1/loaLoa1.2bit SEQ2_LEN=/hive/data/genomes/loaLoa1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/burXyl1/bed/lastzLoaLoa1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 3m31.468s cat fb.burXyl1.chainLoaLoa1Link.txt # 4309744 bases of 73100506 (5.896%) in intersection time (doRecipBest.pl -buildDir=`pwd` burXyl1 loaLoa1) > rbest.log 2>&1 & # real 20m32.806s # and for the swap: mkdir /hive/data/genomes/loaLoa1/bed/blastz.burXyl1.swap cd /hive/data/genomes/loaLoa1/bed/blastz.burXyl1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/burXyl1/bed/lastzLoaLoa1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m50.405s cat fb.loaLoa1.chainBurXyl1Link.txt # 4145414 bases of 87533141 (4.736%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` loaLoa1 burXyl1) \ > rbest.log 2>&1 # real 0m29.698s ######################################################################## ######################################################################## # LASTZ Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 vs. # Onchocerca volvulus/O. volvulus - oncVol1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/burXyl1/bed/lastzOncVol1.2015-08-17 cd /hive/data/genomes/burXyl1/bed/lastzOncVol1.2015-08-17 cat << '_EOF_' > DEF # burXyl1 vs oncVol1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 SEQ1_DIR=/hive/data/genomes/burXyl1/burXyl1.2bit SEQ1_LEN=/hive/data/genomes/burXyl1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Onchocerca volvulus/O. volvulus - oncVol1 SEQ2_DIR=/hive/data/genomes/oncVol1/oncVol1.2bit SEQ2_LEN=/hive/data/genomes/oncVol1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/burXyl1/bed/lastzOncVol1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 3m0.033s cat fb.burXyl1.chainOncVol1Link.txt # 4362593 bases of 73100506 (5.968%) in intersection time (doRecipBest.pl -buildDir=`pwd` burXyl1 oncVol1) > rbest.log 2>&1 & # real 2m39.468s # and for the swap: mkdir /hive/data/genomes/oncVol1/bed/blastz.burXyl1.swap cd /hive/data/genomes/oncVol1/bed/blastz.burXyl1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/burXyl1/bed/lastzOncVol1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m46.084s cat fb.oncVol1.chainBurXyl1Link.txt # 4315151 bases of 94327777 (4.575%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` oncVol1 burXyl1) \ > rbest.log 2>&1 # real 0m40.794s ######################################################################## ######################################################################## # LASTZ Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 vs. # Trichinella spiralis/Trichinella - triSpi1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/burXyl1/bed/lastzTriSpi1.2015-08-17 cd /hive/data/genomes/burXyl1/bed/lastzTriSpi1.2015-08-17 cat << '_EOF_' > DEF # burXyl1 vs triSpi1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 SEQ1_DIR=/hive/data/genomes/burXyl1/burXyl1.2bit SEQ1_LEN=/hive/data/genomes/burXyl1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Trichinella spiralis/Trichinella - triSpi1 SEQ2_DIR=/hive/data/genomes/triSpi1/triSpi1.2bit SEQ2_LEN=/hive/data/genomes/triSpi1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/burXyl1/bed/lastzTriSpi1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 3m25.400s cat fb.burXyl1.chainTriSpi1Link.txt # 2610218 bases of 73100506 (3.571%) in intersection time (doRecipBest.pl -buildDir=`pwd` burXyl1 triSpi1) > rbest.log 2>&1 & # real 2m22.029s # and for the swap: mkdir /hive/data/genomes/triSpi1/bed/blastz.burXyl1.swap cd /hive/data/genomes/triSpi1/bed/blastz.burXyl1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/burXyl1/bed/lastzTriSpi1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m45.807s cat fb.triSpi1.chainBurXyl1Link.txt # 2789875 bases of 58558192 (4.764%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` triSpi1 burXyl1) \ > rbest.log 2>&1 # real 0m24.082s ######################################################################## ######################################################################## # LASTZ Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 vs. # Trichuris suis/Whipworm - triSui1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/burXyl1/bed/lastzTriSui1.2015-08-17 cd /hive/data/genomes/burXyl1/bed/lastzTriSui1.2015-08-17 cat << '_EOF_' > DEF # burXyl1 vs triSui1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 SEQ1_DIR=/hive/data/genomes/burXyl1/burXyl1.2bit SEQ1_LEN=/hive/data/genomes/burXyl1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Trichuris suis/Whipworm - triSui1 SEQ2_DIR=/hive/data/genomes/triSui1/triSui1.2bit SEQ2_LEN=/hive/data/genomes/triSui1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/burXyl1/bed/lastzTriSui1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 3m22.253s cat fb.burXyl1.chainTriSui1Link.txt # 2802111 bases of 73100506 (3.833%) in intersection time (doRecipBest.pl -buildDir=`pwd` burXyl1 triSui1) > rbest.log 2>&1 & # real 4m21.610s # and for the swap: mkdir /hive/data/genomes/triSui1/bed/blastz.burXyl1.swap cd /hive/data/genomes/triSui1/bed/blastz.burXyl1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/burXyl1/bed/lastzTriSui1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m43.922s cat fb.triSui1.chainBurXyl1Link.txt # 2888717 bases of 71825323 (4.022%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` triSui1 burXyl1) \ > rbest.log 2>&1 # real 0m22.764s ########################################################################