######################################################################## # LASTZ Brugia malayi/Filarial worm - bruMal2 vs. # Trichinella spiralis/Trichinella - triSpi1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/bruMal2/bed/lastzTriSpi1.2015-08-17 cd /hive/data/genomes/bruMal2/bed/lastzTriSpi1.2015-08-17 cat << '_EOF_' > DEF # bruMal2 vs triSpi1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Brugia malayi/Filarial worm - bruMal2 SEQ1_DIR=/hive/data/genomes/bruMal2/bruMal2.2bit SEQ1_LEN=/hive/data/genomes/bruMal2/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Trichinella spiralis/Trichinella - triSpi1 SEQ2_DIR=/hive/data/genomes/triSpi1/triSpi1.2bit SEQ2_LEN=/hive/data/genomes/triSpi1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/bruMal2/bed/lastzTriSpi1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 3m42.746s cat fb.bruMal2.chainTriSpi1Link.txt # 2897180 bases of 87424120 (3.314%) in intersection time (doRecipBest.pl -buildDir=`pwd` bruMal2 triSpi1) > rbest.log 2>&1 & # real 0m24.703s # and for the swap: mkdir /hive/data/genomes/triSpi1/bed/blastz.bruMal2.swap cd /hive/data/genomes/triSpi1/bed/blastz.bruMal2.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/bruMal2/bed/lastzTriSpi1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m47.155s cat fb.triSpi1.chainBruMal2Link.txt # 3015360 bases of 58558192 (5.149%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` triSpi1 bruMal2) \ > rbest.log 2>&1 # real 0m24.485s ######################################################################## ######################################################################## # LASTZ Brugia malayi/Filarial worm - bruMal2 vs. # Trichuris suis/Whipworm - triSui1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/bruMal2/bed/lastzTriSui1.2015-08-17 cd /hive/data/genomes/bruMal2/bed/lastzTriSui1.2015-08-17 cat << '_EOF_' > DEF # bruMal2 vs triSui1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Brugia malayi/Filarial worm - bruMal2 SEQ1_DIR=/hive/data/genomes/bruMal2/bruMal2.2bit SEQ1_LEN=/hive/data/genomes/bruMal2/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Trichuris suis/Whipworm - triSui1 SEQ2_DIR=/hive/data/genomes/triSui1/triSui1.2bit SEQ2_LEN=/hive/data/genomes/triSui1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/bruMal2/bed/lastzTriSui1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 4m48.258s cat fb.bruMal2.chainTriSui1Link.txt # 2399958 bases of 87424120 (2.745%) in intersection time (doRecipBest.pl -buildDir=`pwd` bruMal2 triSui1) > rbest.log 2>&1 & # real 0m21.478s # and for the swap: mkdir /hive/data/genomes/triSui1/bed/blastz.bruMal2.swap cd /hive/data/genomes/triSui1/bed/blastz.bruMal2.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/bruMal2/bed/lastzTriSui1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m42.179s cat fb.triSui1.chainBruMal2Link.txt # 2314529 bases of 71825323 (3.222%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` triSui1 bruMal2) \ > rbest.log 2>&1 # real 0m21.462s ########################################################################