######################################################################## # LASTZ Ascaris suum/Pig roundworm - ascSuu1 vs. # Brugia malayi/Filarial worm - bruMal2 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/ascSuu1/bed/lastzBruMal2.2015-08-17 cd /hive/data/genomes/ascSuu1/bed/lastzBruMal2.2015-08-17 cat << '_EOF_' > DEF # ascSuu1 vs bruMal2 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ascaris suum/Pig roundworm - ascSuu1 SEQ1_DIR=/hive/data/genomes/ascSuu1/ascSuu1.2bit SEQ1_LEN=/hive/data/genomes/ascSuu1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Brugia malayi/Filarial worm - bruMal2 SEQ2_DIR=/hive/data/genomes/bruMal2/bruMal2.2bit SEQ2_LEN=/hive/data/genomes/bruMal2/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ascSuu1/bed/lastzBruMal2.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 10m9.851s cat fb.ascSuu1.chainBruMal2Link.txt # 11575168 bases of 262604320 (4.408%) in intersection time (doRecipBest.pl -buildDir=`pwd` ascSuu1 bruMal2) > rbest.log 2>&1 & # real 0m44.029s # and for the swap: mkdir /hive/data/genomes/bruMal2/bed/blastz.ascSuu1.swap cd /hive/data/genomes/bruMal2/bed/blastz.ascSuu1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ascSuu1/bed/lastzBruMal2.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 2m20.695s cat fb.bruMal2.chainAscSuu1Link.txt # 11564837 bases of 87424120 (13.228%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` bruMal2 ascSuu1) \ > rbest.log 2>&1 # real 0m53.594s ######################################################################## ######################################################################## # LASTZ Ascaris suum/Pig roundworm - ascSuu1 vs. # Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/ascSuu1/bed/lastzBurXyl1.2015-08-17 cd /hive/data/genomes/ascSuu1/bed/lastzBurXyl1.2015-08-17 cat << '_EOF_' > DEF # ascSuu1 vs burXyl1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ascaris suum/Pig roundworm - ascSuu1 SEQ1_DIR=/hive/data/genomes/ascSuu1/ascSuu1.2bit SEQ1_LEN=/hive/data/genomes/ascSuu1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 SEQ2_DIR=/hive/data/genomes/burXyl1/burXyl1.2bit SEQ2_LEN=/hive/data/genomes/burXyl1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ascSuu1/bed/lastzBurXyl1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 4m55.347s cat fb.ascSuu1.chainBurXyl1Link.txt # 5228412 bases of 262604320 (1.991%) in intersection time (doRecipBest.pl -buildDir=`pwd` ascSuu1 burXyl1) > rbest.log 2>&1 & # real 0m31.434s # and for the swap: mkdir /hive/data/genomes/burXyl1/bed/blastz.ascSuu1.swap cd /hive/data/genomes/burXyl1/bed/blastz.ascSuu1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ascSuu1/bed/lastzBurXyl1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m50.831s cat fb.burXyl1.chainAscSuu1Link.txt # 4937099 bases of 73100506 (6.754%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` burXyl1 ascSuu1) \ > rbest.log 2>&1 # real 0m33.545s ######################################################################## ######################################################################## # LASTZ Ascaris suum/Pig roundworm - ascSuu1 vs. # Dirofilaria immitis/Dog heartworm - dirImm1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/ascSuu1/bed/lastzDirImm1.2015-08-17 cd /hive/data/genomes/ascSuu1/bed/lastzDirImm1.2015-08-17 cat << '_EOF_' > DEF # ascSuu1 vs dirImm1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ascaris suum/Pig roundworm - ascSuu1 SEQ1_DIR=/hive/data/genomes/ascSuu1/ascSuu1.2bit SEQ1_LEN=/hive/data/genomes/ascSuu1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Dirofilaria immitis/Dog heartworm - dirImm1 SEQ2_DIR=/hive/data/genomes/dirImm1/dirImm1.2bit SEQ2_LEN=/hive/data/genomes/dirImm1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ascSuu1/bed/lastzDirImm1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 6m28.830s cat fb.ascSuu1.chainDirImm1Link.txt # 11594008 bases of 262604320 (4.415%) in intersection time (doRecipBest.pl -buildDir=`pwd` ascSuu1 dirImm1) > rbest.log 2>&1 & # real 0m44.067s # and for the swap: mkdir /hive/data/genomes/dirImm1/bed/blastz.ascSuu1.swap cd /hive/data/genomes/dirImm1/bed/blastz.ascSuu1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ascSuu1/bed/lastzDirImm1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m47.551s cat fb.dirImm1.chainAscSuu1Link.txt # 10969212 bases of 84923352 (12.917%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` dirImm1 ascSuu1) \ > rbest.log 2>&1 # real 6m38.005s ######################################################################## ######################################################################## # LASTZ Ascaris suum/Pig roundworm - ascSuu1 vs. # Loa loa/Eye worm - loaLoa1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/ascSuu1/bed/lastzLoaLoa1.2015-08-17 cd /hive/data/genomes/ascSuu1/bed/lastzLoaLoa1.2015-08-17 cat << '_EOF_' > DEF # ascSuu1 vs loaLoa1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ascaris suum/Pig roundworm - ascSuu1 SEQ1_DIR=/hive/data/genomes/ascSuu1/ascSuu1.2bit SEQ1_LEN=/hive/data/genomes/ascSuu1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Loa loa/Eye worm - loaLoa1 SEQ2_DIR=/hive/data/genomes/loaLoa1/loaLoa1.2bit SEQ2_LEN=/hive/data/genomes/loaLoa1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ascSuu1/bed/lastzLoaLoa1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 9m22.605s cat fb.ascSuu1.chainLoaLoa1Link.txt # 11891288 bases of 262604320 (4.528%) in intersection time (doRecipBest.pl -buildDir=`pwd` ascSuu1 loaLoa1) > rbest.log 2>&1 & # real 0m45.899s # and for the swap: mkdir /hive/data/genomes/loaLoa1/bed/blastz.ascSuu1.swap cd /hive/data/genomes/loaLoa1/bed/blastz.ascSuu1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ascSuu1/bed/lastzLoaLoa1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 8m0.972s cat fb.loaLoa1.chainAscSuu1Link.txt # 11206215 bases of 87533141 (12.802%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` loaLoa1 ascSuu1) \ > rbest.log 2>&1 # real 4m15.590s ######################################################################## ######################################################################## # LASTZ Ascaris suum/Pig roundworm - ascSuu1 vs. # Meloidogyne hapla/M. hapla - melHap1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/ascSuu1/bed/lastzMelHap1.2015-08-17 cd /hive/data/genomes/ascSuu1/bed/lastzMelHap1.2015-08-17 cat << '_EOF_' > DEF # ascSuu1 vs melHap1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ascaris suum/Pig roundworm - ascSuu1 SEQ1_DIR=/hive/data/genomes/ascSuu1/ascSuu1.2bit SEQ1_LEN=/hive/data/genomes/ascSuu1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Meloidogyne hapla/M. hapla - melHap1 SEQ2_DIR=/hive/data/genomes/melHap1/melHap1.2bit SEQ2_LEN=/hive/data/genomes/melHap1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ascSuu1/bed/lastzMelHap1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 3m58.369s cat fb.ascSuu1.chainMelHap1Link.txt # 3012195 bases of 262604320 (1.147%) in intersection time (doRecipBest.pl -buildDir=`pwd` ascSuu1 melHap1) > rbest.log 2>&1 & # real 0m23.086s # and for the swap: mkdir /hive/data/genomes/melHap1/bed/blastz.ascSuu1.swap cd /hive/data/genomes/melHap1/bed/blastz.ascSuu1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ascSuu1/bed/lastzMelHap1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m47.011s cat fb.melHap1.chainAscSuu1Link.txt # 2817207 bases of 53017507 (5.314%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` melHap1 ascSuu1) \ > rbest.log 2>&1 # real 0m26.019s ######################################################################## ######################################################################## # LASTZ Ascaris suum/Pig roundworm - ascSuu1 vs. # Meloidogyne incognita/M. incognita - melInc2 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/ascSuu1/bed/lastzMelInc2.2015-08-17 cd /hive/data/genomes/ascSuu1/bed/lastzMelInc2.2015-08-17 cat << '_EOF_' > DEF # ascSuu1 vs melInc2 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ascaris suum/Pig roundworm - ascSuu1 SEQ1_DIR=/hive/data/genomes/ascSuu1/ascSuu1.2bit SEQ1_LEN=/hive/data/genomes/ascSuu1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Meloidogyne incognita/M. incognita - melInc2 SEQ2_DIR=/hive/data/genomes/melInc2/melInc2.2bit SEQ2_LEN=/hive/data/genomes/melInc2/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ascSuu1/bed/lastzMelInc2.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 4m58.265s cat fb.ascSuu1.chainMelInc2Link.txt # 2927245 bases of 262604320 (1.115%) in intersection time (doRecipBest.pl -buildDir=`pwd` ascSuu1 melInc2) > rbest.log 2>&1 & # real 0m24.102s # and for the swap: mkdir /hive/data/genomes/melInc2/bed/blastz.ascSuu1.swap cd /hive/data/genomes/melInc2/bed/blastz.ascSuu1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ascSuu1/bed/lastzMelInc2.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m51.406s cat fb.melInc2.chainAscSuu1Link.txt # 3937732 bases of 82114761 (4.795%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` melInc2 ascSuu1) \ > rbest.log 2>&1 # real 0m28.204s ######################################################################## ######################################################################## # LASTZ Ascaris suum/Pig roundworm - ascSuu1 vs. # Onchocerca volvulus/O. volvulus - oncVol1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/ascSuu1/bed/lastzOncVol1.2015-08-17 cd /hive/data/genomes/ascSuu1/bed/lastzOncVol1.2015-08-17 cat << '_EOF_' > DEF # ascSuu1 vs oncVol1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ascaris suum/Pig roundworm - ascSuu1 SEQ1_DIR=/hive/data/genomes/ascSuu1/ascSuu1.2bit SEQ1_LEN=/hive/data/genomes/ascSuu1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Onchocerca volvulus/O. volvulus - oncVol1 SEQ2_DIR=/hive/data/genomes/oncVol1/oncVol1.2bit SEQ2_LEN=/hive/data/genomes/oncVol1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ascSuu1/bed/lastzOncVol1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 9m17.351s cat fb.ascSuu1.chainOncVol1Link.txt # 12211021 bases of 262604320 (4.650%) in intersection time (doRecipBest.pl -buildDir=`pwd` ascSuu1 oncVol1) > rbest.log 2>&1 & # real 1m5.528s # and for the swap: mkdir /hive/data/genomes/oncVol1/bed/blastz.ascSuu1.swap cd /hive/data/genomes/oncVol1/bed/blastz.ascSuu1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ascSuu1/bed/lastzOncVol1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 9m44.222s cat fb.oncVol1.chainAscSuu1Link.txt # 11611047 bases of 94327777 (12.309%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` oncVol1 ascSuu1) \ > rbest.log 2>&1 # real 2m17.060s ######################################################################## ######################################################################## # LASTZ Ascaris suum/Pig roundworm - ascSuu1 vs. # Pristionchus exspectatus/P. exspectatus - priExs1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/ascSuu1/bed/lastzPriExs1.2015-08-17 cd /hive/data/genomes/ascSuu1/bed/lastzPriExs1.2015-08-17 cat << '_EOF_' > DEF # ascSuu1 vs priExs1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ascaris suum/Pig roundworm - ascSuu1 SEQ1_DIR=/hive/data/genomes/ascSuu1/ascSuu1.2bit SEQ1_LEN=/hive/data/genomes/ascSuu1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Pristionchus exspectatus/P. exspectatus - priExs1 SEQ2_DIR=/hive/data/genomes/priExs1/priExs1.2bit SEQ2_LEN=/hive/data/genomes/priExs1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ascSuu1/bed/lastzPriExs1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 12m21.782s cat fb.ascSuu1.chainPriExs1Link.txt # 4490517 bases of 262604320 (1.710%) in intersection time (doRecipBest.pl -buildDir=`pwd` ascSuu1 priExs1) > rbest.log 2>&1 & # real 0m42.270s # and for the swap: mkdir /hive/data/genomes/priExs1/bed/blastz.ascSuu1.swap cd /hive/data/genomes/priExs1/bed/blastz.ascSuu1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ascSuu1/bed/lastzPriExs1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 4m44.447s cat fb.priExs1.chainAscSuu1Link.txt # 5058398 bases of 166917800 (3.030%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` priExs1 ascSuu1) \ > rbest.log 2>&1 # real 6m22.662s ######################################################################## ######################################################################## # LASTZ Ascaris suum/Pig roundworm - ascSuu1 vs. # Pristionchus pacificus/P. pacificus - priPac3 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/ascSuu1/bed/lastzPriPac3.2015-08-17 cd /hive/data/genomes/ascSuu1/bed/lastzPriPac3.2015-08-17 cat << '_EOF_' > DEF # ascSuu1 vs priPac3 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ascaris suum/Pig roundworm - ascSuu1 SEQ1_DIR=/hive/data/genomes/ascSuu1/ascSuu1.2bit SEQ1_LEN=/hive/data/genomes/ascSuu1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Pristionchus pacificus/P. pacificus - priPac3 SEQ2_DIR=/hive/data/genomes/priPac3/priPac3.2bit SEQ2_LEN=/hive/data/genomes/priPac3/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ascSuu1/bed/lastzPriPac3.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 8m9.210s cat fb.ascSuu1.chainPriPac3Link.txt # 4567904 bases of 262604320 (1.739%) in intersection time (doRecipBest.pl -buildDir=`pwd` ascSuu1 priPac3) > rbest.log 2>&1 & # real 0m31.017s # and for the swap: mkdir /hive/data/genomes/priPac3/bed/blastz.ascSuu1.swap cd /hive/data/genomes/priPac3/bed/blastz.ascSuu1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ascSuu1/bed/lastzPriPac3.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m3.778s cat fb.priPac3.chainAscSuu1Link.txt # 5000878 bases of 153238914 (3.263%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` priPac3 ascSuu1) \ > rbest.log 2>&1 # real 0m39.244s ######################################################################## ######################################################################## # LASTZ Ascaris suum/Pig roundworm - ascSuu1 vs. # Trichinella spiralis/Trichinella - triSpi1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/ascSuu1/bed/lastzTriSpi1.2015-08-17 cd /hive/data/genomes/ascSuu1/bed/lastzTriSpi1.2015-08-17 cat << '_EOF_' > DEF # ascSuu1 vs triSpi1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ascaris suum/Pig roundworm - ascSuu1 SEQ1_DIR=/hive/data/genomes/ascSuu1/ascSuu1.2bit SEQ1_LEN=/hive/data/genomes/ascSuu1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Trichinella spiralis/Trichinella - triSpi1 SEQ2_DIR=/hive/data/genomes/triSpi1/triSpi1.2bit SEQ2_LEN=/hive/data/genomes/triSpi1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ascSuu1/bed/lastzTriSpi1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 4m44.666s cat fb.ascSuu1.chainTriSpi1Link.txt # 2505975 bases of 262604320 (0.954%) in intersection time (doRecipBest.pl -buildDir=`pwd` ascSuu1 triSpi1) > rbest.log 2>&1 & # real 0m25.569s # and for the swap: mkdir /hive/data/genomes/triSpi1/bed/blastz.ascSuu1.swap cd /hive/data/genomes/triSpi1/bed/blastz.ascSuu1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ascSuu1/bed/lastzTriSpi1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m52.451s cat fb.triSpi1.chainAscSuu1Link.txt # 2498819 bases of 58558192 (4.267%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` triSpi1 ascSuu1) \ > rbest.log 2>&1 # real 3m0.214s ######################################################################## ######################################################################## # LASTZ Ascaris suum/Pig roundworm - ascSuu1 vs. # Trichuris suis/Whipworm - triSui1 # (DONE - 2015-08-17 - Hiram) mkdir /hive/data/genomes/ascSuu1/bed/lastzTriSui1.2015-08-17 cd /hive/data/genomes/ascSuu1/bed/lastzTriSui1.2015-08-17 cat << '_EOF_' > DEF # ascSuu1 vs triSui1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ascaris suum/Pig roundworm - ascSuu1 SEQ1_DIR=/hive/data/genomes/ascSuu1/ascSuu1.2bit SEQ1_LEN=/hive/data/genomes/ascSuu1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Trichuris suis/Whipworm - triSui1 SEQ2_DIR=/hive/data/genomes/triSui1/triSui1.2bit SEQ2_LEN=/hive/data/genomes/triSui1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ascSuu1/bed/lastzTriSui1.2015-08-17 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 4m37.350s cat fb.ascSuu1.chainTriSui1Link.txt # 2756270 bases of 262604320 (1.050%) in intersection time (doRecipBest.pl -buildDir=`pwd` ascSuu1 triSui1) > rbest.log 2>&1 & # real 0m23.455s # and for the swap: mkdir /hive/data/genomes/triSui1/bed/blastz.ascSuu1.swap cd /hive/data/genomes/triSui1/bed/blastz.ascSuu1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ascSuu1/bed/lastzTriSui1.2015-08-17/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m43.750s cat fb.triSui1.chainAscSuu1Link.txt # 2674075 bases of 71825323 (3.723%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` triSui1 ascSuu1) \ > rbest.log 2>&1 # real 0m26.525s ########################################################################