######################################################################## # LASTZ Ancylostoma ceylanicum/A. ceylanicum - ancCey1 vs. # Ascaris suum/Pig roundworm - ascSuu1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/ancCey1/bed/lastzAscSuu1.2015-08-16 cd /hive/data/genomes/ancCey1/bed/lastzAscSuu1.2015-08-16 cat << '_EOF_' > DEF # ancCey1 vs ascSuu1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ancylostoma ceylanicum/A. ceylanicum - ancCey1 SEQ1_DIR=/hive/data/genomes/ancCey1/ancCey1.2bit SEQ1_LEN=/hive/data/genomes/ancCey1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Ascaris suum/Pig roundworm - ascSuu1 SEQ2_DIR=/hive/data/genomes/ascSuu1/ascSuu1.2bit SEQ2_LEN=/hive/data/genomes/ascSuu1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ancCey1/bed/lastzAscSuu1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 9m4.972s cat fb.ancCey1.chainAscSuu1Link.txt # 7800109 bases of 300914990 (2.592%) in intersection time (doRecipBest.pl -buildDir=`pwd` ancCey1 ascSuu1) > rbest.log 2>&1 & # real 0m37.574s # and for the swap: mkdir /hive/data/genomes/ascSuu1/bed/blastz.ancCey1.swap cd /hive/data/genomes/ascSuu1/bed/blastz.ancCey1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ancCey1/bed/lastzAscSuu1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 2m6.163s cat fb.ascSuu1.chainAncCey1Link.txt # 7059281 bases of 262604320 (2.688%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` ascSuu1 ancCey1) \ > rbest.log 2>&1 # real 2m35.144s ######################################################################## ######################################################################## # LASTZ Ancylostoma ceylanicum/A. ceylanicum - ancCey1 vs. # Brugia malayi/Filarial worm - bruMal2 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/ancCey1/bed/lastzBruMal2.2015-08-16 cd /hive/data/genomes/ancCey1/bed/lastzBruMal2.2015-08-16 cat << '_EOF_' > DEF # ancCey1 vs bruMal2 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ancylostoma ceylanicum/A. ceylanicum - ancCey1 SEQ1_DIR=/hive/data/genomes/ancCey1/ancCey1.2bit SEQ1_LEN=/hive/data/genomes/ancCey1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Brugia malayi/Filarial worm - bruMal2 SEQ2_DIR=/hive/data/genomes/bruMal2/bruMal2.2bit SEQ2_LEN=/hive/data/genomes/bruMal2/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ancCey1/bed/lastzBruMal2.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 4m52.887s cat fb.ancCey1.chainBruMal2Link.txt # 5564334 bases of 300914990 (1.849%) in intersection time (doRecipBest.pl -buildDir=`pwd` ancCey1 bruMal2) > rbest.log 2>&1 & # real 0m29.179s # and for the swap: mkdir /hive/data/genomes/bruMal2/bed/blastz.ancCey1.swap cd /hive/data/genomes/bruMal2/bed/blastz.ancCey1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ancCey1/bed/lastzBruMal2.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m37.299s cat fb.bruMal2.chainAncCey1Link.txt # 5524529 bases of 87424120 (6.319%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` bruMal2 ancCey1) \ > rbest.log 2>&1 # real 0m39.436s ######################################################################## ######################################################################## # LASTZ Ancylostoma ceylanicum/A. ceylanicum - ancCey1 vs. # Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/ancCey1/bed/lastzBurXyl1.2015-08-16 cd /hive/data/genomes/ancCey1/bed/lastzBurXyl1.2015-08-16 cat << '_EOF_' > DEF # ancCey1 vs burXyl1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ancylostoma ceylanicum/A. ceylanicum - ancCey1 SEQ1_DIR=/hive/data/genomes/ancCey1/ancCey1.2bit SEQ1_LEN=/hive/data/genomes/ancCey1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Bursaphelenchus xylophilus/Pine wood nematode - burXyl1 SEQ2_DIR=/hive/data/genomes/burXyl1/burXyl1.2bit SEQ2_LEN=/hive/data/genomes/burXyl1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ancCey1/bed/lastzBurXyl1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 6m35.287s cat fb.ancCey1.chainBurXyl1Link.txt # 5944382 bases of 300914990 (1.975%) in intersection time (doRecipBest.pl -buildDir=`pwd` ancCey1 burXyl1) > rbest.log 2>&1 & # real 0m31.539s # and for the swap: mkdir /hive/data/genomes/burXyl1/bed/blastz.ancCey1.swap cd /hive/data/genomes/burXyl1/bed/blastz.ancCey1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ancCey1/bed/lastzBurXyl1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m54.158s cat fb.burXyl1.chainAncCey1Link.txt # 5208033 bases of 73100506 (7.124%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` burXyl1 ancCey1) \ > rbest.log 2>&1 # real 0m38.923s ######################################################################## ######################################################################## # LASTZ Ancylostoma ceylanicum/A. ceylanicum - ancCey1 vs. # Dirofilaria immitis/Dog heartworm - dirImm1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/ancCey1/bed/lastzDirImm1.2015-08-16 cd /hive/data/genomes/ancCey1/bed/lastzDirImm1.2015-08-16 cat << '_EOF_' > DEF # ancCey1 vs dirImm1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ancylostoma ceylanicum/A. ceylanicum - ancCey1 SEQ1_DIR=/hive/data/genomes/ancCey1/ancCey1.2bit SEQ1_LEN=/hive/data/genomes/ancCey1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Dirofilaria immitis/Dog heartworm - dirImm1 SEQ2_DIR=/hive/data/genomes/dirImm1/dirImm1.2bit SEQ2_LEN=/hive/data/genomes/dirImm1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ancCey1/bed/lastzDirImm1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 6m35.273s cat fb.ancCey1.chainDirImm1Link.txt # 5338947 bases of 300914990 (1.774%) in intersection time (doRecipBest.pl -buildDir=`pwd` ancCey1 dirImm1) > rbest.log 2>&1 & # real 0m31.708s # and for the swap: mkdir /hive/data/genomes/dirImm1/bed/blastz.ancCey1.swap cd /hive/data/genomes/dirImm1/bed/blastz.ancCey1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ancCey1/bed/lastzDirImm1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 4m18.258s cat fb.dirImm1.chainAncCey1Link.txt # 5080528 bases of 84923352 (5.982%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` dirImm1 ancCey1) \ > rbest.log 2>&1 # real 4m17.249s ######################################################################## ######################################################################## # LASTZ Ancylostoma ceylanicum/A. ceylanicum - ancCey1 vs. # Haemonchus contortus/Barber pole worm - haeCon2 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/ancCey1/bed/lastzHaeCon2.2015-08-16 cd /hive/data/genomes/ancCey1/bed/lastzHaeCon2.2015-08-16 cat << '_EOF_' > DEF # ancCey1 vs haeCon2 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ancylostoma ceylanicum/A. ceylanicum - ancCey1 SEQ1_DIR=/hive/data/genomes/ancCey1/ancCey1.2bit SEQ1_LEN=/hive/data/genomes/ancCey1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Haemonchus contortus/Barber pole worm - haeCon2 SEQ2_DIR=/hive/data/genomes/haeCon2/haeCon2.2bit SEQ2_LEN=/hive/data/genomes/haeCon2/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ancCey1/bed/lastzHaeCon2.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 17m13.642s cat fb.ancCey1.chainHaeCon2Link.txt # 39375688 bases of 300914990 (13.085%) in intersection time (doRecipBest.pl -buildDir=`pwd` ancCey1 haeCon2) > rbest.log 2>&1 & # real 1m27.111s # and for the swap: mkdir /hive/data/genomes/haeCon2/bed/blastz.ancCey1.swap cd /hive/data/genomes/haeCon2/bed/blastz.ancCey1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ancCey1/bed/lastzHaeCon2.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 8m23.788s cat fb.haeCon2.chainAncCey1Link.txt # 52829603 bases of 346001353 (15.269%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` haeCon2 ancCey1) \ > rbest.log 2>&1 # real 7m34.691s ######################################################################## ######################################################################## # LASTZ Ancylostoma ceylanicum/A. ceylanicum - ancCey1 vs. # Loa loa/Eye worm - loaLoa1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/ancCey1/bed/lastzLoaLoa1.2015-08-16 cd /hive/data/genomes/ancCey1/bed/lastzLoaLoa1.2015-08-16 cat << '_EOF_' > DEF # ancCey1 vs loaLoa1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ancylostoma ceylanicum/A. ceylanicum - ancCey1 SEQ1_DIR=/hive/data/genomes/ancCey1/ancCey1.2bit SEQ1_LEN=/hive/data/genomes/ancCey1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Loa loa/Eye worm - loaLoa1 SEQ2_DIR=/hive/data/genomes/loaLoa1/loaLoa1.2bit SEQ2_LEN=/hive/data/genomes/loaLoa1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ancCey1/bed/lastzLoaLoa1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 9m13.009s cat fb.ancCey1.chainLoaLoa1Link.txt # 6526806 bases of 300914990 (2.169%) in intersection time (doRecipBest.pl -buildDir=`pwd` ancCey1 loaLoa1) > rbest.log 2>&1 & # real 0m32.850s # and for the swap: mkdir /hive/data/genomes/loaLoa1/bed/blastz.ancCey1.swap cd /hive/data/genomes/loaLoa1/bed/blastz.ancCey1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ancCey1/bed/lastzLoaLoa1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 4m0.809s cat fb.loaLoa1.chainAncCey1Link.txt # 5318673 bases of 87533141 (6.076%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` loaLoa1 ancCey1) \ > rbest.log 2>&1 # real 6m1.404s ######################################################################## ######################################################################## # LASTZ Ancylostoma ceylanicum/A. ceylanicum - ancCey1 vs. # Meloidogyne hapla/M. hapla - melHap1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/ancCey1/bed/lastzMelHap1.2015-08-16 cd /hive/data/genomes/ancCey1/bed/lastzMelHap1.2015-08-16 cat << '_EOF_' > DEF # ancCey1 vs melHap1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ancylostoma ceylanicum/A. ceylanicum - ancCey1 SEQ1_DIR=/hive/data/genomes/ancCey1/ancCey1.2bit SEQ1_LEN=/hive/data/genomes/ancCey1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Meloidogyne hapla/M. hapla - melHap1 SEQ2_DIR=/hive/data/genomes/melHap1/melHap1.2bit SEQ2_LEN=/hive/data/genomes/melHap1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ancCey1/bed/lastzMelHap1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 3m30.955s cat fb.ancCey1.chainMelHap1Link.txt # 3367513 bases of 300914990 (1.119%) in intersection time (doRecipBest.pl -buildDir=`pwd` ancCey1 melHap1) > rbest.log 2>&1 & # real 0m22.170s # and for the swap: mkdir /hive/data/genomes/melHap1/bed/blastz.ancCey1.swap cd /hive/data/genomes/melHap1/bed/blastz.ancCey1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ancCey1/bed/lastzMelHap1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m7.314s cat fb.melHap1.chainAncCey1Link.txt # 2968383 bases of 53017507 (5.599%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` melHap1 ancCey1) \ > rbest.log 2>&1 # real 0m26.282s ######################################################################## ######################################################################## # LASTZ Ancylostoma ceylanicum/A. ceylanicum - ancCey1 vs. # Meloidogyne incognita/M. incognita - melInc2 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/ancCey1/bed/lastzMelInc2.2015-08-16 cd /hive/data/genomes/ancCey1/bed/lastzMelInc2.2015-08-16 cat << '_EOF_' > DEF # ancCey1 vs melInc2 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ancylostoma ceylanicum/A. ceylanicum - ancCey1 SEQ1_DIR=/hive/data/genomes/ancCey1/ancCey1.2bit SEQ1_LEN=/hive/data/genomes/ancCey1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Meloidogyne incognita/M. incognita - melInc2 SEQ2_DIR=/hive/data/genomes/melInc2/melInc2.2bit SEQ2_LEN=/hive/data/genomes/melInc2/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ancCey1/bed/lastzMelInc2.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 4m25.502s cat fb.ancCey1.chainMelInc2Link.txt # 3544279 bases of 300914990 (1.178%) in intersection time (doRecipBest.pl -buildDir=`pwd` ancCey1 melInc2) > rbest.log 2>&1 & # real 0m21.913s # and for the swap: mkdir /hive/data/genomes/melInc2/bed/blastz.ancCey1.swap cd /hive/data/genomes/melInc2/bed/blastz.ancCey1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ancCey1/bed/lastzMelInc2.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m53.633s cat fb.melInc2.chainAncCey1Link.txt # 4100632 bases of 82114761 (4.994%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` melInc2 ancCey1) \ > rbest.log 2>&1 # real 0m30.016s ######################################################################## ######################################################################## # LASTZ Ancylostoma ceylanicum/A. ceylanicum - ancCey1 vs. # Necator americanus/N. americanus - necAme1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/ancCey1/bed/lastzNecAme1.2015-08-16 cd /hive/data/genomes/ancCey1/bed/lastzNecAme1.2015-08-16 cat << '_EOF_' > DEF # ancCey1 vs necAme1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ancylostoma ceylanicum/A. ceylanicum - ancCey1 SEQ1_DIR=/hive/data/genomes/ancCey1/ancCey1.2bit SEQ1_LEN=/hive/data/genomes/ancCey1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Necator americanus/N. americanus - necAme1 SEQ2_DIR=/hive/data/genomes/necAme1/necAme1.2bit SEQ2_LEN=/hive/data/genomes/necAme1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ancCey1/bed/lastzNecAme1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 11m41.240s cat fb.ancCey1.chainNecAme1Link.txt # 49659080 bases of 300914990 (16.503%) in intersection time (doRecipBest.pl -buildDir=`pwd` ancCey1 necAme1) > rbest.log 2>&1 & # real 1m42.225s # and for the swap: mkdir /hive/data/genomes/necAme1/bed/blastz.ancCey1.swap cd /hive/data/genomes/necAme1/bed/blastz.ancCey1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ancCey1/bed/lastzNecAme1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 6m34.465s cat fb.necAme1.chainAncCey1Link.txt # 49184225 bases of 208187215 (23.625%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` necAme1 ancCey1) \ > rbest.log 2>&1 # real 8m48.467s ######################################################################## ######################################################################## # LASTZ Ancylostoma ceylanicum/A. ceylanicum - ancCey1 vs. # Onchocerca volvulus/O. volvulus - oncVol1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/ancCey1/bed/lastzOncVol1.2015-08-16 cd /hive/data/genomes/ancCey1/bed/lastzOncVol1.2015-08-16 cat << '_EOF_' > DEF # ancCey1 vs oncVol1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ancylostoma ceylanicum/A. ceylanicum - ancCey1 SEQ1_DIR=/hive/data/genomes/ancCey1/ancCey1.2bit SEQ1_LEN=/hive/data/genomes/ancCey1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Onchocerca volvulus/O. volvulus - oncVol1 SEQ2_DIR=/hive/data/genomes/oncVol1/oncVol1.2bit SEQ2_LEN=/hive/data/genomes/oncVol1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ancCey1/bed/lastzOncVol1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 4m48.677s cat fb.ancCey1.chainOncVol1Link.txt # 5720552 bases of 300914990 (1.901%) in intersection time (doRecipBest.pl -buildDir=`pwd` ancCey1 oncVol1) > rbest.log 2>&1 & # real 0m44.731s # and for the swap: mkdir /hive/data/genomes/oncVol1/bed/blastz.ancCey1.swap cd /hive/data/genomes/oncVol1/bed/blastz.ancCey1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ancCey1/bed/lastzOncVol1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m52.645s cat fb.oncVol1.chainAncCey1Link.txt # 5805676 bases of 94327777 (6.155%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` oncVol1 ancCey1) \ > rbest.log 2>&1 # real 0m46.983s ######################################################################## ######################################################################## # LASTZ Ancylostoma ceylanicum/A. ceylanicum - ancCey1 vs. # Pristionchus exspectatus/P. exspectatus - priExs1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/ancCey1/bed/lastzPriExs1.2015-08-16 cd /hive/data/genomes/ancCey1/bed/lastzPriExs1.2015-08-16 cat << '_EOF_' > DEF # ancCey1 vs priExs1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ancylostoma ceylanicum/A. ceylanicum - ancCey1 SEQ1_DIR=/hive/data/genomes/ancCey1/ancCey1.2bit SEQ1_LEN=/hive/data/genomes/ancCey1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Pristionchus exspectatus/P. exspectatus - priExs1 SEQ2_DIR=/hive/data/genomes/priExs1/priExs1.2bit SEQ2_LEN=/hive/data/genomes/priExs1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ancCey1/bed/lastzPriExs1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 6m12.211s cat fb.ancCey1.chainPriExs1Link.txt # 9246350 bases of 300914990 (3.073%) in intersection time (doRecipBest.pl -buildDir=`pwd` ancCey1 priExs1) > rbest.log 2>&1 & # real 0m36.283s # and for the swap: mkdir /hive/data/genomes/priExs1/bed/blastz.ancCey1.swap cd /hive/data/genomes/priExs1/bed/blastz.ancCey1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ancCey1/bed/lastzPriExs1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m13.947s cat fb.priExs1.chainAncCey1Link.txt # 8834189 bases of 166917800 (5.293%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` priExs1 ancCey1) \ > rbest.log 2>&1 # real 0m53.294s ######################################################################## ######################################################################## # LASTZ Ancylostoma ceylanicum/A. ceylanicum - ancCey1 vs. # Pristionchus pacificus/P. pacificus - priPac3 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/ancCey1/bed/lastzPriPac3.2015-08-16 cd /hive/data/genomes/ancCey1/bed/lastzPriPac3.2015-08-16 cat << '_EOF_' > DEF # ancCey1 vs priPac3 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ancylostoma ceylanicum/A. ceylanicum - ancCey1 SEQ1_DIR=/hive/data/genomes/ancCey1/ancCey1.2bit SEQ1_LEN=/hive/data/genomes/ancCey1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Pristionchus pacificus/P. pacificus - priPac3 SEQ2_DIR=/hive/data/genomes/priPac3/priPac3.2bit SEQ2_LEN=/hive/data/genomes/priPac3/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ancCey1/bed/lastzPriPac3.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 6m37.640s cat fb.ancCey1.chainPriPac3Link.txt # 9588166 bases of 300914990 (3.186%) in intersection time (doRecipBest.pl -buildDir=`pwd` ancCey1 priPac3) > rbest.log 2>&1 & # real 0m37.886s # and for the swap: mkdir /hive/data/genomes/priPac3/bed/blastz.ancCey1.swap cd /hive/data/genomes/priPac3/bed/blastz.ancCey1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ancCey1/bed/lastzPriPac3.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 1m56.145s cat fb.priPac3.chainAncCey1Link.txt # 8549334 bases of 153238914 (5.579%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` priPac3 ancCey1) \ > rbest.log 2>&1 # real 1m26.742s ######################################################################## ######################################################################## # LASTZ Ancylostoma ceylanicum/A. ceylanicum - ancCey1 vs. # Trichinella spiralis/Trichinella - triSpi1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/ancCey1/bed/lastzTriSpi1.2015-08-16 cd /hive/data/genomes/ancCey1/bed/lastzTriSpi1.2015-08-16 cat << '_EOF_' > DEF # ancCey1 vs triSpi1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ancylostoma ceylanicum/A. ceylanicum - ancCey1 SEQ1_DIR=/hive/data/genomes/ancCey1/ancCey1.2bit SEQ1_LEN=/hive/data/genomes/ancCey1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Trichinella spiralis/Trichinella - triSpi1 SEQ2_DIR=/hive/data/genomes/triSpi1/triSpi1.2bit SEQ2_LEN=/hive/data/genomes/triSpi1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ancCey1/bed/lastzTriSpi1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 4m33.827s cat fb.ancCey1.chainTriSpi1Link.txt # 2721325 bases of 300914990 (0.904%) in intersection time (doRecipBest.pl -buildDir=`pwd` ancCey1 triSpi1) > rbest.log 2>&1 & # real 0m23.728s # and for the swap: mkdir /hive/data/genomes/triSpi1/bed/blastz.ancCey1.swap cd /hive/data/genomes/triSpi1/bed/blastz.ancCey1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ancCey1/bed/lastzTriSpi1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m47.051s cat fb.triSpi1.chainAncCey1Link.txt # 2639193 bases of 58558192 (4.507%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` triSpi1 ancCey1) \ > rbest.log 2>&1 # real 0m25.810s ######################################################################## ######################################################################## # LASTZ Ancylostoma ceylanicum/A. ceylanicum - ancCey1 vs. # Trichuris suis/Whipworm - triSui1 # (DONE - 2015-08-16 - Hiram) mkdir /hive/data/genomes/ancCey1/bed/lastzTriSui1.2015-08-16 cd /hive/data/genomes/ancCey1/bed/lastzTriSui1.2015-08-16 cat << '_EOF_' > DEF # ancCey1 vs triSui1 BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_H=2000 BLASTZ_M=50 # TARGET:Ancylostoma ceylanicum/A. ceylanicum - ancCey1 SEQ1_DIR=/hive/data/genomes/ancCey1/ancCey1.2bit SEQ1_LEN=/hive/data/genomes/ancCey1/chrom.sizes SEQ1_CHUNK=4000000 SEQ1_LAP=10000 SEQ1_LIMIT=100 # QUERY: Trichuris suis/Whipworm - triSui1 SEQ2_DIR=/hive/data/genomes/triSui1/triSui1.2bit SEQ2_LEN=/hive/data/genomes/triSui1/chrom.sizes SEQ2_CHUNK=4000000 SEQ2_LIMIT=400 SEQ2_LAP=0 BASE=/hive/data/genomes/ancCey1/bed/lastzTriSui1.2015-08-16 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 -workhorse=hgwdev \ -bigClusterHub=ku -smallClusterHub=ku -syntenicNet) > do.log 2>&1 # real 4m35.060s cat fb.ancCey1.chainTriSui1Link.txt # 4077433 bases of 300914990 (1.355%) in intersection time (doRecipBest.pl -buildDir=`pwd` ancCey1 triSui1) > rbest.log 2>&1 & # real 0m23.368s # and for the swap: mkdir /hive/data/genomes/triSui1/bed/blastz.ancCey1.swap cd /hive/data/genomes/triSui1/bed/blastz.ancCey1.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/ancCey1/bed/lastzTriSui1.2015-08-16/DEF \ -swap -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 0m52.839s cat fb.triSui1.chainAncCey1Link.txt # 3407441 bases of 71825323 (4.744%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` triSui1 ancCey1) \ > rbest.log 2>&1 # real 0m26.499s ########################################################################