### # Plasmodium falciparum 3D7 Version 12 from PlasmoDb # 2014-11-19: markd # This is a temporary genome build for a research project, it may eventually # go to Todd's browser when he has the resources. This will not be pushed # public to the RR. ### cd /hive/data/genomes/plasFalc3D7V12 # download fastas cd data/ wget -nv http://plasmodb.org/common/downloads/release-12.0/Pfalciparum3D7/fasta/data/PlasmoDB-12.0_Pfalciparum3D7_Genome.fasta # create plasFalc3D7V12.config.ra from one of the templates # download http://upload.wikimedia.org/wikipedia/commons/f/f1/Malaria.jpg and # add to tree as htdocs/images/Plasmodium_falciparum_3D7.jpg makeGenomeDb.pl plasFalc3D7V12.config.ra >&make1.log # failed with: mustOpenFd: Can't open ./joinedRmskComposite.ra to read cp -r ~/compbio/browser/dev/kent/src/hg/makeDb/trackDb/joinedRmsk* TemporaryTrackDbCheckout/kent/src/hg/makeDb/trackDb/ makeGenomeDb.pl -continue=trackDb plasFalc3D7V12.config.ra >&make2.log # update defaultDb update defaultDb set name="plasFalc3D7V12" where genome ="Plasmodium falciparum 3D7"; mkdir bed/genes (cd bed/genes && wget -nv http://plasmodb.org/common/downloads/release-12.0/Pfalciparum3D7/gff/data/PlasmoDB-12.0_Pfalciparum3D7.gff) gff3ToGenePred bed/genes/PlasmoDB-12.0_Pfalciparum3D7.gff bed/genes/genes.gp hgLoadGenePred -genePredExt plasFalc3D7V12 genes bed/genes/genes.gp # link in 2bit. We don't have a masked on currently ln -s /hive/data/genomes/plasFalc3D7V12/plasFalc3D7V12.unmasked.2bit /gbdb/plasFalc3D7V12/plasFalc3D7V12.2bit