######################################################################### # LASTZ gorilla/gorGor4 human/hg38 - (DONE - 2016-1-04 - Jonathan) mkdir /hive/data/genomes/hg38/bed/lastzGorGor4.2016-01-04 cd /hive/data/genomes/hg38/bed/lastzGorGor4.2016-01-04 cat << '_EOF_' > DEF # human vs gorilla BLASTZ=/cluster/bin/penn/lastz-distrib-1.03.66/bin/lastz BLASTZ_T=2 BLASTZ_O=600 BLASTZ_E=150 BLASTZ_M=254 BLASTZ_K=4500 BLASTZ_Y=15000 BLASTZ_Q=/scratch/data/blastz/human_chimp.v2.q # A C G T # A 90 -330 -236 -356 # C -330 100 -318 -236 # G -236 -318 100 -330 # T -356 -236 -330 90 # TARGET: human hg38 SEQ1_DIR=/hive/data/genomes/hg38/hg38.2bit SEQ1_LEN=/hive/data/genomes/hg38/chrom.sizes SEQ1_CHUNK=20000000 SEQ1_LAP=10000 SEQ1_IN_CONTIGS=0 # QUERY: gorilla gorGor4 SEQ2_DIR=/hive/data/genomes/gorGor4/gorGor4.2bit SEQ2_LEN=/hive/data/genomes/gorGor4/chrom.sizes SEQ2_CHUNK=20000000 SEQ2_LAP=0 SEQ2_LIMIT=500 SEQ_IN_CONTIGS=0 BASE=/hive/data/genomes/hg38/bed/lastzGorGor4.2016-01-04 TMPDIR=/dev/shm '_EOF_' # << happy emacs time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 \ -chainMinScore=3000 -chainLinearGap=medium \ -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > do.log 2>&1 # real 233m13.670s cat fb.hg38.chainGorGor4Link.txt # 2831582533 bases of 3049335806 (92.859%) in intersection time (doRecipBest.pl -buildDir=`pwd` hg38 gorGor4) > rbest.log 2>&1 & # real 41m52.877s # and for the swap: mkdir /hive/data/genomes/gorGor4/bed/blastz.hg38.swap cd /hive/data/genomes/gorGor4/bed/blastz.hg38.swap time (doBlastzChainNet.pl -verbose=2 \ /hive/data/genomes/hg38/bed/lastzGorGor4.2016-01-04/DEF \ -swap -chainMinScore=3000 -chainLinearGap=medium \ -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 105m40.710s cat fb.gorGor4.chainHg38Link.txt # 2731892128 bases of 2917333143 (93.643%) in intersection time (doRecipBest.pl -buildDir=`pwd` gorGor4 hg38) > rbest.log 2>&1 # real 44m25.268s #########################################################################