############################################################################## # SWAP from chimp/rheMac8 Lastz run (DONE - 2016-08-27 - Hiram) # primary alignment cd /hive/data/genomes/rheMac8/bed/lastzChlSab2.2016-08-27 cat fb.rheMac8.chainChlSab2Link.txt # 2674926475 bases of 3142093174 (85.132%) in intersection # running the swap mkdir /hive/data/genomes/chlSab2/bed/blastz.rheMac8.swap cd /hive/data/genomes/chlSab2/bed/blastz.rheMac8.swap time (doBlastzChainNet.pl -verbose=2 \ -swap /hive/data/genomes/rheMac8/bed/lastzChlSab2.2016-08-27/DEF \ -chainMinScore=5000 -chainLinearGap=medium \ -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 # real 89m19.998s cat fb.chlSab2.chainRheMac8Link.txt # 2596129666 bases of 2752019208 (94.335%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` \ chlSab2 rheMac8) > rbest.log 2>&1 # real 278m9.306s ############################################################################# # chimp/rheMac2 <-> Chlorocebus sabaeus/Green monkey Lastz run (DONE - 2015-01-28 - Brian) screen -S chlSab2RheMac2 # use a screen to manage this longish running job mkdir /hive/data/genomes/chlSab2/bed/lastzRheMac2.2015-01-28 cd /hive/data/genomes/chlSab2/bed/lastzRheMac2.2015-01-28 # always set the BLASTZ program so we know what version was used printf "# vervet vs macaca mulatta BLASTZ=lastz # maximum M allowed with lastz is only 254 BLASTZ_M=254 BLASTZ_Q=/scratch/data/blastz/human_chimp.v2.q # and place those items here BLASTZ_O=600 BLASTZ_E=150 # other parameters from panTro2 vs hg18 lastz on advice from Webb BLASTZ_K=4500 BLASTZ_Y=15000 BLASTZ_T=2 # TARGET: Human Hg19 SEQ1_DIR=/hive/data/genomes/chlSab2/chlSab2.2bit SEQ1_LEN=/hive/data/genomes/chlSab2/chrom.sizes SEQ1_CHUNK=10000000 SEQ1_LAP=10000 SEQ1_IN_CONTIGS=0 # QUERY: Macaca Mulatta RheMac2 SEQ2_DIR=/scratch/data/rheMac2/rheMac2.2bit SEQ2_LEN=/scratch/data/rheMac2/chrom.sizes SEQ2_CHUNK=20000000 SEQ2_LAP=0 SEQ2_IN_CONTIGS=0 BASE=/hive/data/genomes/chlSab2/bed/lastzRheMac2.2015-01-28 TMPDIR=/scratch/tmp " > DEF time (doBlastzChainNet.pl `pwd`/DEF -verbose=2 \ -chainMinScore=5000 -chainLinearGap=medium \ -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > do.log 2>&1 # Elapsed time: 346m35s cat fb.hg38.chainChlSab2Link.txt # 2480180858 bases of 2752019208 (90.122%) in intersection # filter with doRecipBest.pl doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` \ hg38 chlSab2 # about 45 minutes # running the swap mkdir /hive/data/genomes/rheMac2/bed/blastz.chlSab2.swap cd /hive/data/genomes/rheMac2/bed/blastz.chlSab2.swap time (doBlastzChainNet.pl -verbose=2 \ -swap /hive/data/genomes/chlSab2/bed/lastzRheMac2.2015-01-28/DEF \ -chainMinScore=5000 -chainLinearGap=medium \ -workhorse=hgwdev -smallClusterHub=ku -bigClusterHub=ku \ -syntenicNet) > swap.log 2>&1 XXX - running - Wed Sep 28 10:01:36 PDT 2016 # Elapsed time: 88m48s cat fb.chlSab2.chainHg38Link.txt # 2429053010 bases of 2752019208 (88.264%) in intersection time (doRecipBest.pl -workhorse=hgwdev -buildDir=`pwd` \ chlSab2 hg38) > rbest.log 2>&1 # real 240m25.264s #############################################################################