LOCUS       SYNPUC19CV              2686 bp    DNA     circular SYN 17-MAY-2019
DEFINITION  Cloning vector pUC19c, complete sequence.
ACCESSION   L09137
VERSION     L09137.2
KEYWORDS    .
SOURCE      Cloning vector pUC19c
  ORGANISM  Cloning vector pUC19c
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Yanisch-Perron,C., Vieira,J. and Messing,J.
  TITLE     Improved M13 phage cloning vectors and host strains: nucleotide
            sequences of the M13mp18 and pUC19 vectors
  JOURNAL   Gene 33 (1), 103-119 (1985)
  PUBMED    2985470
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Chambers,S.P., Prior,S.E., Barstow,D.A. and Minton,N.P.
  TITLE     The pMTL nic- cloning vectors. I. Improved pUC polylinker regions
            to facilitate the use of sonicated DNA for nucleotide sequencing
  JOURNAL   Gene 68 (1), 139-149 (1988)
  PUBMED    2851488
REFERENCE   3  (bases 1 to 2686)
  AUTHORS   Gilbert,W.
  TITLE     Obtained from VecBase 3.0
  JOURNAL   Unpublished
REFERENCE   4  (bases 1 to 2686)
  AUTHORS   Messing,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-APR-1993) Department of Biochemistry, University of
            Minnesota, St. Paul, MN 55108, USA
REFERENCE   5  (bases 1 to 2686)
  AUTHORS   Messing,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2002) Rutgers, The State University of New
            Jersey, Waksman Institute of Microbiology, 190 Frelinghuysen Road,
            Piscataway, NJ 08854-8020, USA
  REMARK    Sequence update by submitter
COMMENT     On Apr 11, 2002 this sequence version replaced L09137.1. These data
            and their annotation were supplied to GenBank by Will    Gilbert
            under the auspices of the GenBank Currator Program. pUC19c  -
            Cloning vector (beta-galactosidase mRNA on complementary strand)
            ENTRY PUC19C                    #TYPE DNA CIRCULAR TITLE pUC19c -
            Cloning vector
                          (beta-galactosidase mRNA on complementary strand)
            DATE      03-FEB-1986
               #sequence  16-DEC-1986
            ACCESSION VB0033
            SOURCE    artificial
            COLLECTION  ATCC 37254
            REFERENCE
               #number
               #authors  Norrander J., Kempe T., Messing J.
               #journal Gene (1983) 26: 101-106
            REFERENCE
               #number  1
               #authors  Yanisch-Perron C., Vieira J., Messing J.
               #journal Gene (1985) 33: 103-119
               #comment shows the complete compiled sequence
            REFERENCE
               #number  2
               #authors  Chambers,S.P., et al.
               #journal Gene (1988) 68: 139-149
               #describes mutation at nt1308 and its effect on copy number
            REFERENCE
               #number
               #authors Pouwels P.H., Enger-Valk B.E., Brammar W.J.
               #book    Cloning Vectors, Elsvier 1985 and supplements
               #comment  vector I-A-iv-20
            COMMENT
               This Sequence was obtained 3-MAR-1986 from J. Messing, Waksman
               Institute, NJ on floppy disk.
               Revised 16-DEC-1986 by F. Pfeiffer:
               1062/3 'AT' to 'TA' to match revised sequence of PBR322
               The beta-galactosidase mRNA sequence including the multiple
               cloning site of M13mp19 is on the strand complementary to that
               shown.
            KEYWORDS
            CROSSREFERENCE
               #complement
                 VecBase(3):pUC19
               #prerevised
                 GenBank(50):M11662, EMBL(11):ARPuc19
               #parent
                 VecBase(3):pUC13, VecBase(3):M13mp19, VecSource(3):bGal19
            PARENT
               Features of pUC19c (2686 bp)
                 residue     source
                  1- 137  2074-2210 pBR322
                138- 237  2252-2351 pBR322
                238- 395  1461-1304 (c) Lac-Operon
                396- 452    57-   1 (c) polylinker of M13mp19
                455- 682  1298-1071 (c) Lac-Operon
                683-2686  2352-4355 pBR322
               Conflict (cfl) and Mutations (mut):
                    pUC19c  source
               mut 1308  A  G  2977   pBR322   linked to increased copy number
               mut 1942  A  G  3611   pBR322
               mut 2243  T  C  3912   pBR322
            FEATURE
               1629-2417 789-1 (c) Ap-R
            b-lactamase                            POLYLINKER
            HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-KpnI-SacI-EcoRI  SELECTION

            #resistance  Ap
            #indicator beta-galactosidase
            SUMMARY  pUC19c    #length 2686   #checksum 4465.
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Cloning vector pUC19c"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:174689"
     promoter        complement(514..543)
                     /standard_name="lac prom"
     promoter        complement(2528..2556)
                     /standard_name="amp prom"
     misc_feature    <2644..159
                     /standard_name="cross origin trunc"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      421 cctctagagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
      481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      601 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      661 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
      721 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      781 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      841 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      901 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      961 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1021 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1081 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1141 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1201 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1261 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     1321 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1381 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1441 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1501 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1561 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1621 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     1681 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     1741 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     1801 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     1861 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     1921 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     1981 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     2041 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     2101 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     2161 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     2221 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     2281 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     2341 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     2401 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     2461 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     2521 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     2581 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     2641 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
