Dc@sdZidd6dd6dd6dd6Zid d 6d d 6d d6dd6dd6Zidd 6dd6Zidd6dd6dd6dd6dd6dd6d d6Zd$Zd!d%d"YZd#S(&s.Calculate isoelectric points of polypeptides using methods of Bjellqvist. pK values and the methos are taken from: * Bjellqvist, B.,Hughes, G.J., Pasquali, Ch., Paquet, N., Ravier, F., Sanchez, J.-Ch., Frutiger, S. & Hochstrasser, D.F. The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences. Electrophoresis 1993, 14, 1023-1031. * Bjellqvist, B., Basse, B., Olsen, E. and Celis, J.E. Reference points for comparisons of two-dimensional maps of proteins from different human cell types defined in a pH scale where isoelectric points correlate with polypeptide compositions. Electrophoresis 1994, 15, 529-539. I designed the algorithm according to a note by David L. Tabb, available at: http://fields.scripps.edu/DTASelect/20010710-pI-Algorithm.pdf g@tNtermg$@tKg(@tRgQ@tHgffffff @tCtermg333333@tDg@tEg"@tCtYg333333@g@g\(\@tAg@tMgQ@tSgQ @tPgHzG@tTg(\@tVg@tIsoelectricPointcBs,eZdZdZdZdZRS(cCs||_|j||_dS(N(tsequencet_select_chargedtcharged_aas_content(tselftProteinSequencetAminoAcidsContent((s/oak/stanford/groups/akundaje/marinovg/programs/biopython-1.50.tar.gz/biopython-1.50/build/lib.linux-x86_64-2.7/Bio/SeqUtils/IsoelectricPoint.pyt__init__ s cCsCi}x"tD]}t||||s ")7