ó ćۧHc@s9dZddd„ƒYZddd„ƒYZd„ZdS( s+Code to interact with the primer3 program. tRecordcBseZdZd„ZRS(sŒRepresent information from a primer3 run finding primers. Members: primers A list of primers that are generated (usually 5) cCsd|_g|_dS(Nt(tcommentstprimers(tself((s…/oak/stanford/groups/akundaje/marinovg/programs/biopython-1.50.tar.gz/biopython-1.50/build/lib.linux-x86_64-2.7/Bio/Emboss/Primer3.pyt__init__ s (t__name__t __module__t__doc__R(((s…/oak/stanford/groups/akundaje/marinovg/programs/biopython-1.50.tar.gz/biopython-1.50/build/lib.linux-x86_64-2.7/Bio/Emboss/Primer3.pyRstPrimerscBseZdZd„ZRS(säA primer set designed by Primer3. Members: size forward_seq forward_start forward_length forward_tm forward_gc reverse_seq reverse_start reverse_length reverse_tm reverse_gc cCsgd|_d|_d|_d|_d|_d|_d|_d|_d|_d|_ d|_ dS(NiRg( tsizet forward_seqt forward_starttforward_lengtht forward_tmt forward_gct reverse_seqt reverse_starttreverse_lengtht reverse_tmt reverse_gc(R((s…/oak/stanford/groups/akundaje/marinovg/programs/biopython-1.50.tar.gz/biopython-1.50/build/lib.linux-x86_64-2.7/Bio/Emboss/Primer3.pyR"s          (RRRR(((s…/oak/stanford/groups/akundaje/marinovg/programs/biopython-1.50.tar.gz/biopython-1.50/build/lib.linux-x86_64-2.7/Bio/Emboss/Primer3.pyR scCs,t|ƒ}tƒ}d }x|D]}|jƒr"Pq"q"Wx_tr|jƒrf|ddk rfPn|j|7_y|jƒ}Wq?tk r™|SXq?Wx‡tr'|jƒsśnI|dd!dkrřtƒ}t |dƒ|_ |j j |ƒn|dd!dkrĽ|j ƒ}| s-|j dkrItƒ}|j j |ƒnt |dƒ|_t |dƒ|_t|d ƒ|_t|dƒ|_|d |_nZ|dd!d krR|j ƒ}| sÚ|j dkrötƒ}|j j |ƒnt |dƒ|_t |dƒ|_t|d ƒ|_t|dƒ|_|d |_n­|dd!d kr˙|j ƒ}| s‡|j dkrŁtƒ}|j j |ƒnt |dƒ|_t |dƒ|_t|d ƒ|_t|dƒ|_|d |_ny|jƒ}WqĄtk r#PqĄXqĄW|S(s/Parse primer3 output into a Primer3Record. it#iisPRODUCT SIZE: sFORWARD PRIMERiiiisREVERSE PRIMERsINTERNAL OLIGON(titerRtNonetstriptTrueRtnextt StopIterationR tintR RtappendtsplitR R tfloatRRR RRRRRtinternal_starttinternal_lengtht internal_tmt internal_gct internal_seq(thandletrecordtprimertlinetwords((s…/oak/stanford/groups/akundaje/marinovg/programs/biopython-1.50.tar.gz/biopython-1.50/build/lib.linux-x86_64-2.7/Bio/Emboss/Primer3.pytread/sp                  N(((RRR R*(((s…/oak/stanford/groups/akundaje/marinovg/programs/biopython-1.50.tar.gz/biopython-1.50/build/lib.linux-x86_64-2.7/Bio/Emboss/Primer3.pyts