dissoc[iate] -sam[ple] <sample name>

                 -amp[licon] <amplicon name> [-ofRef <reference sequence name>]

                 [-file <file> [-format <format>]]

    dissoc[iate] -sam[ple] <sample name>

                 -readData <read data name>

                 [-file <file> [-format <format>]]

    dissoc[iate] -sam[ple] <sample name>

                 -amp[licon] <amplicon name> [-ofRef <reference sequence name>]

                 -readData <read data name>

                 [-file <file> [-format <format>]]

    dissoc[iate] -mul[tiplexer] <multiplexer name>

                 [-primer1Mid <primer1Mid name> 

                     [-ofPrimer1MidGroup <primer1MidGroup name>]]

                 [-primer2Mid <primer2Mid name> 

                     [-ofPrimer2MidGroup <primer2MidGroup name>]]

                 [-file <file> [-format <format>]]

    dissoc[iate] -mul[tiplexer] <multiplexer name>

                 [-primer1Mid <primer1Mid name> 

                     [-ofPrimer1MidGroup <primer1MidGroup name>]]

                 [-primer2Mid <primer2Mid name> 

                     [-ofPrimer2MidGroup <primer2MidGroup name>]]

                 -sam[ple] <sample name>

                 [-file <file> [-format <format>]]

    dissoc[iate] -mul[tiplexer] <multiplexer name>

                 -sam[ple] <sample name>

                 [-file <file> [-format <format>]]

    dissoc[iate] -mul[tiplexer] <multiplexer name>

                 -amp[licon] <amplicon name> [-ofRef <reference sequence name>]

                 -readData <readData name>

                 [-file <file> [-format <format>]]

    dissoc[iate] -mul[tiplexer] <multiplexer name>

                 -readData <readData name>

                 [-file <file> [-format <format>]]

    The 'dissociate' command is used to dissociate records in many-to-many

    relationships.  If a general relationship is dissociated, the more 

    specific associations that depend on it automatically will be dissociated

    as well (e.g., dissociating a sample from a read data will also result in

    the dissociation of the sample's read data-sample-amplicon associations).

    General relationships that are included as part of specific relationships

    are not, however, automatically dissociated (e.g., when dissociating a

    read data-sample-amplicon relationship, the more general sample-amplicon

    relationship that it includes will not be dissociated).

    In any of the command forms above where '-amplicon' is being specified, 

    the '-ofRef' option can be used to disambiguate amplicons with the same 

    name but which are from different reference sequences.

    The '-amplicon' option may be specified as a "*" to allow multiple 

    amplicons to be dissociated with a single command.  In the context of a 

    command where '-amplicon' and '-sample' are both specified, the "*" is 

    interpreted to indicate that all of the amplicons of the sample should be 

    dissociated.  In the context of a command where '-amplicon', 

    '-multiplexer', and '-readData' are all specified, the "*" is interpreted 

    to indicate that all of the amplicons associated with the multiplexer in 

    the context of that read data should be dissociated.  In either case, the 

    '-ofRef' option can still be used to restrict the "*" selection of 

    amplicons to be that subset of amplicons belonging to the indicated 

    reference sequence.

    In a similar manner to the '-ofRef' option for amplicons, the 

    '-ofPrimer1MidGroup' and '-ofPrimer2MidGroup' options can be used to 

    disambiguate '-primer1Mid' and '-primer2Mid' specifications, respectively. 

    The '-primer1Mid' and '-primer2Mid' options may also be specified as a 

    "*".  If no '-ofPrimer1MidGroup' or '-ofPrimer2MidGroup' option is

    supplied, the "*" refers to all the MIDs of the project.  If a MID group

    is specified, the "*" refers to only the MIDs of that MID group. 

    Explanations of the various command forms are as follows:

    Run 'help general tabularCommands' for information about the '-file'

    option.

    dissoc[iate] -sam[ple] <sample name>

                 -amp[licon] <amplicon name> 

                 [-ofRef <reference sequence name>]

                 [-file <file> [-format <format>]]

    If a sample and an amplicon are specified, they are dissociated.  The

    '-ofRef' option can be used to disambiguate amplicons with the same name

    that refer to different reference sequences.  If a "*" is passed as the

    '-amplicon' option value, with no '-ofRef' option, all amplicons of the

    sample are dissociated.  If both a "*" and the '-ofRef' option are used,

    then all amplicons of the sample belonging to the indicated reference

    sequence will be dissociated.

    dissoc[iate] -sam[ple] <sample name>

                 -readData <read data name>

                 [-file <file> [-format <format>]]

    If a sample and a read data are specified, the sample itself, all 

    amplicons of the sample currently associated with the read data, and the 

    read data are dissociated.

    dissoc[iate] -sam[ple] <sample name>

                 -amp[licon] <amplicon name> 

                 [-ofRef <reference sequence name>]

                 -readData <read data name>

                 [-file <file> [-format <format>]]

    If a sample, amplicon, and read data are specified, the sample itself, the

    amplicon, and the read data are dissociated.  The '-ofRef' option can be

    used to disambiguate amplicons with the same name that refer to different

    reference sequences.  If a "*" is passed as the '-amplicon' option value,

    all amplicons of the sample are dissociated.  This is identical to using

    the invocation form with only the sample and read data specified.

    dissoc[iate] -mul[tiplexer] <multiplexer name>

                 [-primer1Mid <primer1Mid name> 

                     [-ofPrimer1MidGroup <primer1MidGroup name>]]

                 [-primer2Mid <primer2Mid name> 

                     [-ofPrimer2MidGroup <primer2MidGroup name>]]

                 [-file <file> [-format <format>]]

    If some combination of MIDs and a multiplexer are specified, the MIDs will

    be dissociated from the multiplexer.  Any samples associated with those 

    MIDs via the multiplexer will be dissociated as well.  Note that a 

    multiplexer may be used on more than one read data, and MID dissociations

    will impact sample associations on all of those read data at once.  Also 

    note, that depending on the pre-existing sample associations and 

    encoding type of the multiplexer (both, either, primer1, or primer2), 

    dissociating an MID might impact more than one sample (e.g., if the 

    multiplexer encoding is 'both' and there is a sample1 associated with 

    primer1Mid mid1 and primer2Mid mid2 and there is a sample2 associated with

    primer1Mid mid1 and primer2Mid mid3, dissociating primer1 mid1 from the 

    multiplexer will cause both samples to be dissociated). 

    dissoc[iate] -mul[tiplexer] <multiplexer name>

                 [-primer1Mid <primer1Mid name> 

                     [-ofPrimer1MidGroup <primer1MidGroup name>]]

                 [-primer2Mid <primer2Mid name> 

                     [-ofPrimer2MidGroup <primer2MidGroup name>]]

                 -sam[ple] <sample name>

                 [-file <file> [-format <format>]]

    If some combination of MIDs, a multiplexer, and a sample are specified, 

    the sample will be dissociated from the specific MID association, but the

    MID associations with the multiplexer will be left intact.  The 

    '-primer1Mid' and '-primer2Mid' options are constrained by the encoding

    type of the multiplexer.  Since this form of the command is expecting to

    dissociate specific sample-MID associations, it must be given an 

    appropriate combination of MID options that are compatible with the

    encoding type.  If the encoding type is 'both', '-primer1Mid' and 

    '-primer2Mid' options must both be specified along with the sample.  

    If the encoding type is 'either', it is permissible to provide both MIDs

    or just a single MID along with the specified sample.

    dissoc[iate] -mul[tiplexer] <multiplexer name>

                 -sam[ple] <sample name>

                 [-file <file> [-format <format>]]

    If a multiplexer and a sample are specified, the sample gets dissociated 

    from all MID combinations that have been used to associate the sample 

    with the multiplexer.  The pre-existing multiplexer-MID associations are 

    left intact.

    dissoc[iate] -mul[tiplexer] <multiplexer name>

                 -amp[licon] <amplicon name> [-ofRef <reference sequence name>]

                 -readData <readData name>

                 [-file <file> [-format <format>]]

    If a multiplexer, amplicon and readData are specified, the amplicon is 

    dissociated from the specific read data-multiplexer context.  If the 

    amplicon is simultaneously associated with the same multiplexer on a 

    different read data, that relationship will be left intact.  A "*" may be 

    provided with the '-amplicon' option to indicate that all amplicons 

    associated with the read data-multiplexer should be dissociated.  The 

    '-ofRef' option can be used, if necessary, to disambiguate among amplicons 

    with the same name or to restrict the "*" set of amplicons to those of 

    the specified reference sequence.  Severing the relationship of an

    amplicon with a read data-multiplexer simultaneously dissociates the

    amplicon from all of the samples associated with the multiplexer in that

    read data context.  The general sample-amplicon relationships, however,

    remain intact.

    dissoc[iate] -mul[tiplexer] <multiplexer name>

                 -readData <readData name>

                 [-file <file> [-format <format>]]

    If a multiplexer and a read data are specified, the multiplexer will be 

    dissociated from that specific read data.  The internal relationships of 

    the multiplexer such as MID and sample associations remain intact, but any 

    amplicons that were associated with the specific read data-multiplexer 

    will be dissociated.  If the multiplexer is simultaneously associated with

    other specific read data, those associations remain unchanged.

© 2011 454 Life Sciences Corporation, a Roche Company.

For life science research only. Not for use in diagnostic procedures.