2. Example Amplicon Project Design and Analysis
:
2.5 Important Factors in the Assessment of New Variants
: 2.5.4 Homopolymers
2.5.4
Homopolymers
If your potential Variant results from the overcall or undercall of a homopolymer, the length of the homopolymer and the sequence context will impact your assessment of the Variant. As homopolymer length increases, it becomes more likely that an erroneous overcall or undercall will occur. If there is a homopolymer of the same nucleotide in close proximity upstream or downstream of the one impacted by the Variant, known sequencing artifacts called carry-forward and incomplete extension could have caused the undercall or overcall.
