2. Example Amplicon Project Design and Analysis
:
2.5 Important Factors in the Assessment of New Variants
: 2.5.2 Coverage
2.5.2
Coverage
If the depth of coverage at the potential Variant position is very low, a low frequency variant becomes less believable. At higher coverage, low frequency events become more believable provided they are convincingly above the noise. In general, one would want sufficient coverage to see several concrete instances of the variation. However, at extremely high coverage, you should be aware that identical noise type events can occur more than once, and seeing a small number of variant instances in such a case would not necessarily provide convincing evidence.
