2. Example Amplicon Project Design and Analysis
: 2.5 Important Factors in the Assessment of New Variants
2.5
Important Factors in the Assessment of New Variants
2.5.1 Above the Noise
2.5.2 Coverage
2.5.3 Bidirectional Support
2.5.4 Homopolymers
2.5.5 Flowgram Evidence
2.5.6 Read Length
The examples above clearly show that variations observed in the reads of a sequencing experiment should be given careful scrutiny before they can be considered to be true Variants, existing physically in the DNA sample that was sequenced. This section enumerates and briefly describes some of the main features of the data to examine when making this kind of assessment.
