The next step is to launch the GS Amplicon Variant Analyzer (AVA) application, which is done from the command line, using the “gsAmplicon” command (see section 1.1.2); the AVA software splash screen appears briefly, while the application is launching, and then the AVA main window is displayed with its Overview tab showing the AVA introduction text and the 7 main buttons, to the right of the window; the ‘Project Name’ and ‘Location’ fields at the top left are initially blank and all the other tabs are grayed-out, since no project is open (see Figure 2‑2).