Navigation on the Flowgrams Tab

1. GS Amplicon Variant Analyzer Application : 1.8 The Flowgrams Tab : 1.8.3 Navigation on the Flowgrams Tab

1.8.3

There are two navigation controls located at the upper-left corner of the tab that allow you to select new flowgrams to display in the Flowgrams tab. The first is a drop down menu (see Figure 1‑66, above) that contains a list of all the reads that are present in the source tab that generated the one currently displayed (the Global Align or the Consensus Align tab). Selecting a new read from the drop down menu will update the Flowgrams tab with the tri-flowgram for the new read, replacing the current data. This allows you to quickly compare the flowgrams of various reads over the data group available (e.g. all the reads of a Sample, for a single or a given set of Amplicon(s), as displayed in the Global Align tab; or a sub-list of the reads of a given consensus, as restricted by one or more selection(s) applied on the Consensus Align tab).

The second Read display control allows you for even faster scanning of the set of reads. It consists in a pair of arrow buttons located just below the ‘Read’ drop down menu. Clicking one of these buttons directly replaces the tri-flowgram currently displayed by the one of the read next to (or preceding) it in the list. This update is very fast and allows you to quickly scan the flowgrams for a large number of reads to see if a variation (most readily seen on the difference flowgram) is rare or, on the contrary, present in many (or most) of them.