The Global Align tab allows you to view the underlying alignment information that is used in the calculation of Variant frequencies. It is divided into two results panels (Figure 1‑57): the top panel contains a stacked histogram / depth of coverage plot of all the variations observed in the reads included in the last computation, relative to the Reference Sequence while the bottom panel contains the multiple alignment of those reads to the Reference Sequence. In addition, a set of display option tools (for data navigation and filtering) as well as a “Mouse Tracker” display (section 1.1.3.3.3) and color legend for the Variation Frequency Plot are available on the left-hand side of the tab. The Global Align tab can only display data for one Sample-Reference Sequence combination at a time, but it can display data for multiple Amplicons together, provided that they are all associated with both this Sample and this Reference Sequence.