5. cDNA / Transcriptome Sequencing Appendix
5.
cDNA / Transcriptome Sequencing Appendix
5.1 Introduction to cDNA Sequencing Analysis
5.2 Transcriptome Assembly Concepts
5.3 Transcriptome Mapping Concepts
5.4 Reference Type Auto Detection
5.1
Introduction to cDNA Sequencing Analysis
cDNA is produced from the RNA (often, mRNA) in a sample. The cDNA is then sequenced resulting in reads that represent the original sample’s mRNA. These reads can then be mapped against one or more references to gain information about the representation of individual mRNAs and genes from which the mRNAs are transcribed and their level of variation in the original sample. Alternatively, the reads can be assembled together to discover novel transcripts and splice variants for those mRNAs represented by multiple overlapping reads.
