4. GS De Novo Assembler and GS Reference Mapper Appendices
:
4.8 Paired End Libraries in the 454 Sequencing System
: 4.8.2 Ends of Paired End Reads Shorter Than 50 bp (Tags)
4.8.2
Ends of Paired End Reads Shorter Than 50 bp (Tags)
Halves of Paired End reads shorter than 50 bp are treated separately from longer reads in the mapping and assembly computations. These are referred to by the software as ‘Tags’. The minimum length of tags to be used in the computation can be adjusted using “-minlen” command line option or the
Minimum read length
parameter in the GUI (the default is 20 bp, the allowed value range is 15-45).
