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2. GS Reference Mapper : 2.6 Add/Remove Read Data and References with the Project Tab : 2.6.2 Removing Read or Reference Data from the project Sub-Tabs
The Remove button removes the selected Reference and/or Read Data files from the list, but does not immediately remove the file(s) from the project’s sff sub-directory or from any computed mapping results. Actual removal of the files from the project (and of the reads they contain from existing alignments), occurs only when the mapping is re-computed (see section 2.8.2).