Control DNA Tab Features and Functionalities

3.8.1

The Control DNA tab contains many features with the functionalities described below (see Figure 38):

•	The Options area (on the top left area of the tab):

◦	Control DNA: choice of data to display:

▪	All Control DNA sequences included in the Run

▪	Any of the specific Control DNA sequences included in the Run

▪	Unrecognized reads, i.e. reads which begin with the Control DNA sequencing key (GACT, CATG or ATGC; see Section 7.1 for details) but do not match any of the corresponding Control DNA reference sequences

◦	Base Pairs: choice of length over which to calculate match, i.e. show % match from the first base after the key up to this nucleotide in the reads. Only the options relevant to the read length of the data set are displayed.

◦

A button to display the consensus flowgrams for each of the specific Control DNA sequences (as selected) in a tri-flowgram viewer (Figure 39). See Section 3.8.2 for a complete description of the Control DNA consensus flowgram view. This button is available only if the current selection is one of the Control DNA sequences; and grayed out if “all” or “unrecognized” are selected.

Figure 39: The “Display Consensus Flowgram” button of the Control DNA tab, available when one of the Control DNA sequences is selected

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Navigation and data capture buttons: Except for the “Display Consensus Flowgram” button described above, all the buttons on this tab have common functions, allowing a user to save the data as a text file or snapshot image. See Section 3.3.4 for a description of the navigation and data capture button functions.

•	Plot display:

◦	Graphic display of the % match data for all key passed Control DNA reads, per the options selected for the individual regions of the PicoTiterPlate Device and as an aggregate average.

◦	This data is presented at 3 levels of accuracy:

▪	100%: The percent of key passed Control DNA reads that exactly matched the first N-Nucleotide cycles of the corresponding known reference sequence.

▪	≥ 98%: The percent of key passed Control DNA reads that had no more than 2% basecalling differences in the first N-Nucleotide cycles compared to their known reference sequence.

▪	≥ 95%: The percent of key passed Control DNA reads that had no more than 5% basecalling differences in the first N-Nucleotide cycles compared to their known reference sequence.

◦	The plot shares the common scrolling and zooming functions of the other GS Run Browser plots See Section 3.3.3 for a description of the plot functions.

◦

When the mouse pointer is over the plot, the usual mouse tracker shows the x-axis value, and the % of reads among the Control DNA sequences selected that matched their respective reference sequences at 100, 98, and 95% accuracy over the selected length, in the region under the pointer or averaged over the entire PTP Device.

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Summary: This area displays the number of Raw Wells and Control DNA reads for the Control DNA species specified; it also displays the percentage of matches between these reads and their reference sequence at the read length specified, for each region of the PicoTiterPlate Device and as an aggregate average.