The Control DNA tab (Figure 38) displays accuracy results for the Control DNA Beads that were spiked in the sequencing reaction, when available (see the Sequencing Method Manual for details about this procedure). Specifically, the Control DNA tab reports the percentage of Control DNA reads that match their reference sequence at 95%, 98%, and 100% accuracy, in each region of the PicoTiterPlate Device. It also allows a user to view the “consensus flowgram” for the reads from each Control DNA sequence. (A consensus flowgram is the flowgram constructed by averaging the signals of the reads that matched the reference sequence over all flows, for the selected region or the entire PicoTiterPlate Device.)

For the Control DNA tab data to be displayed, the region.cwf files (or, for the GS FLX standard chemistry, files 454RunTimeMetricsAll.txt and 454QualityFilterMetrics.txt) must be present in the Analysis sub-directory of the sequencing Run. For GS FLX standard chemistry data sets, the region.wells files must also exist for the consensus flowgrams to be available.