The Signals Tab

3.6	The Signals Tab

3.6.1 Signals Tab Features and Functionalities

The Signals tab (Figure 36) provides statistics on the distribution of the signals recorded during a Run, and how they were interpreted by the data processing software in terms of the number of base incorporations at a given signal intensity. The tab displays either library well signals (sequencing key TCAG or GACT) or Control DNA well signals (sequencing key CATG or ATGC; see Section 7.1 for details), in one of the following three modes:

•	Raw Intensity: the signal intensities as found in the region.cwf (or region.wells) files, using all the key passed wells for the library or Control DNA wells.

•	Raw Intensity (Filtered): the same signal intensity values, but using only the wells that both key passed and passed all quality filters.

•	Signals (N-mer): the basecalling estimates for the signal intensities, where the Raw Intensity (Filtered) signal values are converted into the estimated numbers of nucleotides that were incorporated in each well during the flow selected (for filter pass wells only).

The information displayed on this tab is not normally used for the evaluation of a sequencing Run, but it can prove useful as an advanced tool to troubleshoot problems.

Figure 36: The Signals tab shows a plot of the number of wells that produce final (passed filter) library reads and those reads interpreted by the data processing software as the extension of a given number of nucleotides.

For the Signals tab to be displayed, the region.cwf (or region.wells) file(s) located in the “regions” sub-folder of the “D_” data analysis folder of the sequencing Run must exist. If no region.cwf (or region.wells) files exist for the Run, the Signals tab will be unavailable.