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3. GS Run Browser : 3.5 The Wells Tab : 3.5.4 Location Flowgrams
3.5.4
Location Flowgrams
Selecting the Location flowgram action from the right click menu from anywhere in the main image area brings up a “raw” flowgram window for that location (Figure 34). The flowgram is constructed by computing, for each image in the sequencing Run the average raw (non-corrected) signal intensity for the 9 pixels surrounding the selected location, and plotting these averages against the succession of reagent flows. (Note that this calculation does not give any consideration to the notion of “wells”.)
Figure 34: The Location flowgram of the Wells tab
 
Only one Location flowgram window can be open at a time. Selecting the Location flowgram action for another location on the main image display will replace any existing data in a Location flowgram window. However, both a Location flowgram window and a Well flowgram or Tri-flowgram window can be open together.
 
3.5.4.1
Location Flowgram Features and Functionalities
The Location flowgram window is very similar to the Well flowgram window (Section 3.5.2). The only four differences are:
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The window title bar identifies it as containing a Location flowgram.
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The flowgram plot displays raw signal intensities instead of corrected intensities.
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There isn’t an option to display N-mers since these cannot be computed from non-normalized (corrected) data.
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A Show all check box allows a user to display (checked) or hide (unchecked) the flows that are neither nucleotides nor PPi/ATP flows.