4. GS Run Browser
: 4.2 Processing Run Data
4.2
Processing Run Data
Data processing jobs not configured as a part of the sequencing Run can be launched post-Run on the GS FLX+ Instrument, from the GS Run Browser. On the ‘Overview’ tab, locate the Run Processor Manager tool in the lower right-hand portion of the Overview tab (
Figure 36
). This tool can also be used to reprocess data sets that were processed previously; each round of processing will give rise to its own D_ directory.
Figure 36: GS Run Processor Manager Tool in the GS Run Browser Overview tab
To launch a data processing job:
•
Type in a unique name for the processing job - This name will be appended to the data processing directory name (D_...) and the folder of processed results will be placed inside the sequencing Run folder (R_...), parallel to any existing data processing folders.
•
Select the processing type. The options below are the standard choices shipped with the software, but your site may have additional options.
◦
If you opened an R_ directory, the options presented will be:
▪
Image Processing Only
▪
Full Processing for Shotgun or Paired End
▪
Full Processing for Amplicons.
◦
If you opened a D_ directory, the options presented will be:
▪
Signal Processing for Shotgun or Paired End
▪
Signal Processing for Amplicons.
•
Click the ‘Start’ button. There is a ‘Stop’ button if the processing job needs to be halted for any reason.
Once a job has been launched, the progress and statistics of the job is displayed, as shown on
Figure 37
.
Figure 37: GS Run Processor Manager Tool job-in-progress statistics
The gsRunProcessor.log file can be viewed in a separate pop-up window by clicking the ‘Messages’ button. The button is enabled for data processing jobs in progress or completed.
Once a processing job has been completed, the results can be loaded into the GS Run Browser for viewing by clicking the ‘Open’ button. See Part B, Section 3 of this manual for details on how to use the GS Run Browser to examine the results of a sequencing Run.
