Data Tab

3.5

The ‘Data’ tab is used to manage the sequencing Run data stored on the GS FLX+ Instrument. The components of the ‘Data’ tab include (Figure 29):

•	four Data set action buttons on the left of the tab – ‘Copy’, ‘Remove’, ‘Backup’ and ‘Browse’

•	a Data set list view (expandable to all sub-folders and files) in the center of the tab

•	a ‘Data Set’ summary panel on the right of the tab, providing details and context for the selected data set.

Figure 29: The GS Sequencer application Data tab

The data set list allows the Operator to select a sequencing data set, by Run name, for further action and to see a list of all the folders and files that were created during the Run. The list can be sorted by name or date by right-clicking on the list and selecting the appropriate sort. Once a data set is selected in the data set list, information and context about the Run are displayed to the right (including the full Run name, which is also used to name the Run ‘R_...’ directory; see Section 3.6).

A new sequencing Run cannot be launched if the estimated disk space of the new Run is not available in the $RIGDATA directory.

The total size of the raw image data generated from a 400 cycle GS FLX Titanium sequencing Run is ~35GB. This amount of data can quickly fill up the GS FLX+ Instrument data partition. Hence it is very important to remove Run data from the instrument disk once it has been archived and transferred to an appropriate storage location. Before a Run can be started, the Instrument Procedure Wizard verifies if there is enough space for the Run and the selected data processing. If there is not enough space, the Instrument Procedure Wizard will show an error message and prevent initiation of a Run until more disk space is made available.

The action buttons on the left fulfill the following functions:

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Copy – Clicking the ‘Copy’ button for a selected sequencing Run data set (an R_... folder) copies the folder and its contents to a networked computer. A dialog box will open that displays a list of pre-configured network locations. The Operator can select the desired destination for the data set copy. (For details on configuration of networked computers, see the GS FLX+ System SysAdmin Guide.)

Figure 30: Copy Data Set dialog box

•	Individual GS Run Processor data files (D_... folders) cannot be copied using the ‘Copy’ button.

•	If the copy destination is designated in the form user@host:path the copy progress indicator on the Data tab will not update with detailed progress during the coping phase. Once the copy has completed the status will update to complete.

•	Remove – Clicking the ‘Remove’ button deletes the selected Run folder (R_...) from the GS FLX+ Instrument data file system. Removal of sequencing Run data sets is only allowed for files that have a backup status of ‘Complete’ or ‘None’.

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Backup – Clicking on the ‘Backup’ button for a selected sequencing Run data set (R_... folder) invokes the backupScript.sh script that archives the selected data set to a data storage location on the network. This is the same backup function as can be applied to a Run in advance, during Run set up (see section 3.3.2, step 8).

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Browse – Clicking the ‘Browse’ button launches the GS Run Browser for viewing the results of a sequencing Run. If a Run folder is selected (R_...), Run and image information is available. If a data processing folder is selected (D_...), signal intensities, read length distribution and filter metrics are also available. See Section 3 for an overview, and Part B, Section 3 of this manual for details on the GS Run Browser application.

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The Run data set is not removed from the GS FLX+ Instrument data file system by the ‘Copy’ or the ‘Backup’ buttons. Copy is used to transfer the data to an appropriate network location, e.g. for further analysis; while Backup is used for archival purposes. The two actions are distinct and separate. Copying a Run data set will not change its Backup status.

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The ‘Copy’, ‘Remove’ and ‘Backup’ functions can be applied to multiple data sets at the same time by selecting multiple data sets in the file list. To select multiple items, press and hold the control key and click on the list items of interest. To select an adjacent group of items on the list, click on the first item then press and hold the shift key and click on the last item of interest.