3. GS Sequencer
: 3.4 Instrument Tab
3.4
Instrument Tab
The ‘Instrument’ tab of the GS Sequencer application is divided into an upper and a lower area (previously seen, e.g. in
Figure 7
, above).
Figure 27
shows how the Instrument tab looks when a Run is in progress.
Figure 27: The GS Sequencer application Instrument tab during a sequencing Run
In the upper panel of the ‘Instrument’ tab, the Procedure section contains the following real-time information on the sequencing Run:
•
Run data – as entered in the Instrument Procedure Wizard (Run name, Run Group, Kit type, PTP ID & type, number of cycles, whether a backup of the data is scheduled, and the Run Script)
•
Run launch time – time the Run was started
•
Run events – a scrollable list of messages and warnings issued by the instrument during the sequencing Run
•
A progress bar for the reagent flows
•
A thumbnail of the last image captured during the Run
The Run Processor section on the right side of the Instrument tab contains the following information for the data processing in progress:
•
A progress bar for the processing steps and an information icon that can be clicked to get more detailed information on the processing steps.
•
The type of data processing that is being done on the Instrument
•
Processing Metrics –Total raw and key pass wells, for all regions of the PTP device, for library as well as for control reads.
The lower area of the ‘Instrument’ tab displays the readings from the various sensors that monitor the status of the GS FLX+ Instrument components. The components and their status data are listed below:
•
PicoTiterPlate (PTP) Device – holds the DNA sample
◦
ID is the number detected by reading the data matrix on the PTP device. This may or may not be the actual number entered while setting up the instrument procedure.
◦
Door Status – ‘Door Closed’, ‘Door Open’
◦
Door Lock/Unlock Button (same functionality also available in the Instrument Procedure Wizard)
◦
PTP Heater Temperature indicator bar
•
Camera – captures the images of the PTP device during the nucleotide flows
◦
CCD Temperature indicator bar (°C)
◦
Back Plate Temperature indicator bar (°C)
◦
Camera Pressure indicator bar (Torr)
•
Cassette – feeds reagents to the fluidics system through the sipper
◦
Enzyme Chiller Temperature indicator bar (°C)
◦
Sipper Status – ‘Lowered’, ‘Raised’
◦
Cassette type – ‘Pre-wash’, ‘Reagent’ or not present
For a wash procedure (pre-wash or maintenance wash) the ‘Instrument’ tab shows the progress of the procedure (
Figure 28
).
Figure 28: The GS Sequencer application Instrument tab during a Maintenance Wash
