Before You Begin

3. GS Sequencer : 3.3 Starting a Sequencing Run : 3.3.1 Before You Begin

3.3.1

Before You Begin

There are three topics that need to be addressed before configuring and launching a sequencing Run using the GS Sequencer application Instrument Procedure Wizard:

•	the GS FLX+ Instrument must be ready and available (see the GS FLX+ Instrument Owner’s Manual for more details).

•	the sequencing Run lab materials must be available (see the Sequencing Method Manual, GS FLX Titanium Series, and the Tables of Materials Required But Not Provided for more details).

•	the Operator must decide whether or not to use a data processing pipeline, and if so, an appropriate pipeline for the sequencing Run to be chosen as part of the Run setup (see Part B, Section 1 - GS Run Processor - in this manual for more details).

While the default installation contains a default Operator and Run Groups that allow you to sign-in and perform a sequencing Run directly, you may want to set up additional Operators and Run Groups via the ‘Config’ button (see Section 3.7.2).

The GS FLX+ Instrument is designed for continuous operation. Under normal circumstances, Operators launching sequencing Runs do not perform any shutdown procedures. All run scripts proceed autonomously after a sequencing Run is launched and each Run is completed by a fluidics maintenance wash after the full number of cycles of data have been collected. Sequencing Runs can take up to 24 hours to complete, and the used PTP device is left in place throughout the pre-wash of the next sequencing Run. The laboratory method details of launching a sequencing Run, including loading of the PTP device, the Reagents cassette, etc., are discussed in the Sequencing Method Manual.