#Chromosome	Start	End	Name	GUIDE-Seq Reads	Strand	Cells	Targetsite	Target_Sequence	Offtarget_Sequence	20 bp protospacer # mismatches	3 bp PAM # mismatches	Mismatch Total
chr2	73160983	73161004	tru_EMX1_chr2_73160998_7539	7539	+	U2OS	tru_EMX1	GTCCGAGCAGAAGAAGAANGG	GTCCGAGCAGAAGAAGAAGGG	0	0	0
chr6	9118792	9118813	tru_EMX1_chr6_9118799_189	189	-	U2OS	tru_EMX1	GTCCGAGCAGAAGAAGAANGG	GTCTGAGCAGAAGAAGAATGG	1	0	1
chr3	95690179	95690200	tru_EMX1_chr3_95690185_28	28	-	U2OS	tru_EMX1	GTCCGAGCAGAAGAAGAANGG	ATCCAAGCAGAAGAAGAAGAG	2	1	3
chr1	151027591	151027612	tru_EMX1_chr1_151027597_12	12	-	U2OS	tru_EMX1	GTCCGAGCAGAAGAAGAANGG	CTCCAAGCAGAAGAAGAAGAG	2	1	3