[M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4842, 288279, 4321, 4742) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1642, 3819, 6488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16180) [M::mem_pestat] mean and std.dev: (4168.36, 2869.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21026) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 247, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (257.28, 95.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1952, 3992, 6412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15332) [M::mem_pestat] mean and std.dev: (4323.91, 2740.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19792) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1775, 3826, 6496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15938) [M::mem_pestat] mean and std.dev: (4228.63, 2815.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20659) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 478.330 CPU sec, 66.645 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4838, 288064, 4427, 4528) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1552, 3727, 6488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16360) [M::mem_pestat] mean and std.dev: (4139.98, 2890.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21296) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 249, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (258.74, 95.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1926, 4099, 6661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16131) [M::mem_pestat] mean and std.dev: (4399.41, 2791.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20866) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1674, 3789, 6387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15813) [M::mem_pestat] mean and std.dev: (4176.58, 2826.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20526) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 391.864 CPU sec, 27.896 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4617, 289688, 4422, 4794) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1726, 3836, 6538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16162) [M::mem_pestat] mean and std.dev: (4226.82, 2863.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20974) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 249, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (259.00, 95.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1937, 4036, 6494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15608) [M::mem_pestat] mean and std.dev: (4320.04, 2743.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20165) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1826, 3920, 6510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15878) [M::mem_pestat] mean and std.dev: (4259.82, 2827.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20562) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 388.840 CPU sec, 26.839 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4605, 289129, 4199, 4767) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1648, 3752, 6476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16132) [M::mem_pestat] mean and std.dev: (4166.33, 2892.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20960) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 249, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (258.94, 95.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1957, 4012, 6728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16270) [M::mem_pestat] mean and std.dev: (4387.52, 2778.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21041) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1825, 4036, 6581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16093) [M::mem_pestat] mean and std.dev: (4288.75, 2841.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20849) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 385.739 CPU sec, 25.082 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4849, 287945, 4378, 4681) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1575, 3758, 6470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16260) [M::mem_pestat] mean and std.dev: (4142.11, 2870.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21155) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 249, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (259.86, 96.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1881, 4100, 6679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16275) [M::mem_pestat] mean and std.dev: (4403.24, 2802.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21073) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1751, 3904, 6431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15791) [M::mem_pestat] mean and std.dev: (4236.82, 2799.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20471) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 398.330 CPU sec, 26.964 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4784, 288288, 4332, 4621) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1660, 3885, 6483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16129) [M::mem_pestat] mean and std.dev: (4190.07, 2851.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 249, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (259.52, 96.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1839, 4126, 6931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17115) [M::mem_pestat] mean and std.dev: (4464.51, 2863.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22207) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1871, 3941, 6548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15902) [M::mem_pestat] mean and std.dev: (4291.76, 2806.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20579) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 396.920 CPU sec, 25.375 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4636, 287827, 4371, 4823) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1615, 3851, 6497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16261) [M::mem_pestat] mean and std.dev: (4174.93, 2877.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21143) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (260.75, 96.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1878, 4102, 6752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16500) [M::mem_pestat] mean and std.dev: (4418.72, 2809.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21374) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1692, 3744, 6445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15951) [M::mem_pestat] mean and std.dev: (4162.63, 2841.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20704) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 392.719 CPU sec, 25.383 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4786, 287070, 4498, 4780) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1518, 3751, 6556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16632) [M::mem_pestat] mean and std.dev: (4122.50, 2877.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 251, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (261.86, 97.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1943, 4132, 6767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16415) [M::mem_pestat] mean and std.dev: (4462.44, 2820.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21239) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1788, 3939, 6410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15654) [M::mem_pestat] mean and std.dev: (4241.72, 2798.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20276) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 388.931 CPU sec, 24.966 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4891, 285833, 4484, 4749) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1545, 3650, 6395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16095) [M::mem_pestat] mean and std.dev: (4069.64, 2870.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (261.51, 97.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1874, 4036, 6857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16823) [M::mem_pestat] mean and std.dev: (4431.88, 2839.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21806) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1858, 3958, 6458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15658) [M::mem_pestat] mean and std.dev: (4256.89, 2783.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20258) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 395.624 CPU sec, 25.808 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4724, 285096, 4377, 4829) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1587, 3669, 6426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16104) [M::mem_pestat] mean and std.dev: (4103.09, 2841.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 252, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (262.88, 98.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1868, 4015, 6610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16094) [M::mem_pestat] mean and std.dev: (4327.02, 2791.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20836) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1772, 3840, 6459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15833) [M::mem_pestat] mean and std.dev: (4222.15, 2826.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20520) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 389.337 CPU sec, 25.354 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4919, 285390, 4400, 4723) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1673, 3831, 6446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15992) [M::mem_pestat] mean and std.dev: (4147.75, 2842.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 252, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (262.73, 97.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1962, 4108, 6778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16410) [M::mem_pestat] mean and std.dev: (4452.19, 2820.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21226) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1813, 3883, 6473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15793) [M::mem_pestat] mean and std.dev: (4241.97, 2813.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20453) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 394.394 CPU sec, 25.219 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4792, 285169, 4387, 4846) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1646, 3722, 6432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16004) [M::mem_pestat] mean and std.dev: (4107.87, 2829.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 253, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (263.55, 98.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1949, 4204, 6779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16439) [M::mem_pestat] mean and std.dev: (4482.07, 2839.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21269) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1753, 3955, 6488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15958) [M::mem_pestat] mean and std.dev: (4256.63, 2820.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20693) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 392.353 CPU sec, 25.196 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4777, 284281, 4297, 4717) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1661, 3781, 6483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16127) [M::mem_pestat] mean and std.dev: (4153.31, 2856.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 253, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (263.97, 98.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1940, 4044, 6737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16331) [M::mem_pestat] mean and std.dev: (4401.79, 2814.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21128) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1653, 3723, 6445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16029) [M::mem_pestat] mean and std.dev: (4163.46, 2820.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20821) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 392.748 CPU sec, 25.563 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4858, 284021, 4420, 4675) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1573, 3731, 6387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16015) [M::mem_pestat] mean and std.dev: (4098.14, 2867.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 253, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (263.59, 98.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1944, 4067, 6638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16026) [M::mem_pestat] mean and std.dev: (4373.70, 2743.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20720) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1642, 3717, 6389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15883) [M::mem_pestat] mean and std.dev: (4135.05, 2806.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20630) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 394.817 CPU sec, 25.368 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4867, 284787, 4410, 4630) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1597, 3723, 6457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16177) [M::mem_pestat] mean and std.dev: (4136.57, 2888.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21037) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 252, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (263.53, 98.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1932, 4052, 6654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16098) [M::mem_pestat] mean and std.dev: (4387.11, 2777.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20820) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1780, 3957, 6378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15574) [M::mem_pestat] mean and std.dev: (4250.99, 2811.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20172) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 390.382 CPU sec, 25.083 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4894, 282563, 4385, 4786) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1480, 3605, 6484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16492) [M::mem_pestat] mean and std.dev: (4056.65, 2903.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 251, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (261.70, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1978, 4131, 6658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16018) [M::mem_pestat] mean and std.dev: (4433.20, 2784.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20698) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1766, 4009, 6518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16022) [M::mem_pestat] mean and std.dev: (4284.30, 2803.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20774) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 427.060 CPU sec, 27.511 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4698, 283405, 4275, 4751) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1550, 3712, 6459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16277) [M::mem_pestat] mean and std.dev: (4111.35, 2889.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21186) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 253, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (264.43, 99.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1942, 3985, 6577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15847) [M::mem_pestat] mean and std.dev: (4357.60, 2785.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20482) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1790, 3933, 6545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16055) [M::mem_pestat] mean and std.dev: (4258.19, 2798.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20810) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 400.705 CPU sec, 25.611 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4806, 282240, 4434, 4768) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1641, 3821, 6488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16182) [M::mem_pestat] mean and std.dev: (4163.06, 2846.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21029) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 255, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (265.54, 100.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1972, 4085, 6757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16327) [M::mem_pestat] mean and std.dev: (4440.59, 2793.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21112) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1773, 3898, 6516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16002) [M::mem_pestat] mean and std.dev: (4252.04, 2826.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20745) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 396.245 CPU sec, 25.211 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4990, 282097, 4407, 4843) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1713, 3809, 6419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15831) [M::mem_pestat] mean and std.dev: (4154.38, 2824.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 254, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (265.66, 100.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1903, 3967, 6663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16183) [M::mem_pestat] mean and std.dev: (4342.89, 2795.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20943) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1656, 3691, 6409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15915) [M::mem_pestat] mean and std.dev: (4117.80, 2804.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20668) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 400.106 CPU sec, 25.595 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4841, 281941, 4373, 4705) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1715, 3816, 6446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15908) [M::mem_pestat] mean and std.dev: (4156.71, 2831.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 254, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (265.13, 99.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1874, 4046, 6761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16535) [M::mem_pestat] mean and std.dev: (4397.19, 2825.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21422) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1767, 3929, 6507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15987) [M::mem_pestat] mean and std.dev: (4264.95, 2830.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20727) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 403.984 CPU sec, 25.799 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4904, 281919, 4523, 4733) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1549, 3654, 6400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16102) [M::mem_pestat] mean and std.dev: (4051.99, 2855.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 254, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (265.59, 100.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1900, 4155, 6840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16720) [M::mem_pestat] mean and std.dev: (4459.18, 2859.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21660) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1719, 3792, 6309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15489) [M::mem_pestat] mean and std.dev: (4171.14, 2821.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20079) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 396.974 CPU sec, 25.359 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4952, 282318, 4504, 4647) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1522, 3635, 6240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15676) [M::mem_pestat] mean and std.dev: (4025.12, 2836.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 254, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (265.11, 99.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1858, 3975, 6443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15613) [M::mem_pestat] mean and std.dev: (4282.82, 2750.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20198) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1691, 3911, 6429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15905) [M::mem_pestat] mean and std.dev: (4202.11, 2820.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20643) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 403.879 CPU sec, 25.693 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4739, 280774, 4391, 4832) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1568, 3794, 6518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16418) [M::mem_pestat] mean and std.dev: (4121.44, 2875.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 254, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (265.41, 99.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1909, 3994, 6634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16084) [M::mem_pestat] mean and std.dev: (4366.67, 2787.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20809) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1826, 3932, 6528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15932) [M::mem_pestat] mean and std.dev: (4267.44, 2837.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20634) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 408.597 CPU sec, 26.043 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4894, 282479, 4335, 4728) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1546, 3608, 6384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16060) [M::mem_pestat] mean and std.dev: (4069.93, 2885.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20898) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 254, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (264.99, 99.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1997, 4103, 6709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16133) [M::mem_pestat] mean and std.dev: (4421.84, 2770.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20845) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1803, 3861, 6524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15966) [M::mem_pestat] mean and std.dev: (4252.28, 2832.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 400.088 CPU sec, 25.514 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4906, 281516, 4365, 4710) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1567, 3762, 6495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16351) [M::mem_pestat] mean and std.dev: (4123.34, 2905.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21279) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 254, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (265.17, 99.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1903, 3949, 6673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16213) [M::mem_pestat] mean and std.dev: (4347.88, 2793.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20983) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1746, 3850, 6493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15987) [M::mem_pestat] mean and std.dev: (4224.99, 2830.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20734) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 406.903 CPU sec, 25.980 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4904, 278784, 4150, 4466) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1472, 3635, 6468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16460) [M::mem_pestat] mean and std.dev: (4052.23, 2909.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 252, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (262.52, 97.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1976, 4107, 6474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15470) [M::mem_pestat] mean and std.dev: (4385.90, 2760.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19968) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1650, 3809, 6429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15987) [M::mem_pestat] mean and std.dev: (4168.67, 2853.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20766) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 450.125 CPU sec, 29.609 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4783, 282564, 4349, 4678) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1562, 3792, 6389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16043) [M::mem_pestat] mean and std.dev: (4087.13, 2865.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 253, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (263.93, 98.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1898, 4015, 6576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15932) [M::mem_pestat] mean and std.dev: (4355.37, 2773.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20610) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1732, 3796, 6469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15943) [M::mem_pestat] mean and std.dev: (4211.82, 2846.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20680) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 405.505 CPU sec, 25.914 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4771, 281290, 4358, 4779) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1436, 3608, 6371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16241) [M::mem_pestat] mean and std.dev: (4043.69, 2920.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21176) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 254, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (264.91, 99.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1934, 3985, 6513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15671) [M::mem_pestat] mean and std.dev: (4357.99, 2769.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20250) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1755, 3882, 6382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15636) [M::mem_pestat] mean and std.dev: (4189.63, 2809.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20263) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 412.057 CPU sec, 27.631 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4974, 281254, 4249, 4567) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1530, 3698, 6441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16263) [M::mem_pestat] mean and std.dev: (4089.89, 2885.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21174) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 254, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (264.22, 98.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1893, 3990, 6534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15816) [M::mem_pestat] mean and std.dev: (4326.63, 2772.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20457) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1758, 3816, 6393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15663) [M::mem_pestat] mean and std.dev: (4196.93, 2815.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20298) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 409.168 CPU sec, 26.154 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4886, 280621, 4347, 4684) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1550, 3601, 6307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15821) [M::mem_pestat] mean and std.dev: (4045.60, 2864.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 253, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (264.09, 98.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1925, 3917, 6735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16355) [M::mem_pestat] mean and std.dev: (4370.61, 2810.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21165) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1732, 3782, 6450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15886) [M::mem_pestat] mean and std.dev: (4191.24, 2838.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20604) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 404.822 CPU sec, 25.896 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4855, 280691, 4335, 4728) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1553, 3698, 6474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16316) [M::mem_pestat] mean and std.dev: (4102.12, 2888.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21237) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 255, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (267.30, 104.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1938, 4076, 6840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16644) [M::mem_pestat] mean and std.dev: (4432.51, 2796.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21546) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1796, 3884, 6444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15740) [M::mem_pestat] mean and std.dev: (4232.60, 2815.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20388) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 406.256 CPU sec, 25.864 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4948, 279280, 4446, 4867) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1508, 3722, 6431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16277) [M::mem_pestat] mean and std.dev: (4071.19, 2878.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21200) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 257, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (269.35, 105.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1908, 3925, 6540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15804) [M::mem_pestat] mean and std.dev: (4318.94, 2788.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20436) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1779, 3835, 6373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15561) [M::mem_pestat] mean and std.dev: (4208.66, 2799.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20155) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 394.699 CPU sec, 25.107 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4808, 280299, 4480, 4840) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1555, 3726, 6453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16249) [M::mem_pestat] mean and std.dev: (4113.23, 2876.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21147) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 257, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (269.85, 106.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1950, 4119, 6767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16401) [M::mem_pestat] mean and std.dev: (4449.31, 2807.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21218) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1814, 3912, 6471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15785) [M::mem_pestat] mean and std.dev: (4241.82, 2812.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20442) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 386.383 CPU sec, 24.690 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4749, 279836, 4373, 4883) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1569, 3751, 6347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15903) [M::mem_pestat] mean and std.dev: (4086.01, 2840.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 257, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (269.01, 105.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1897, 4081, 6748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16450) [M::mem_pestat] mean and std.dev: (4408.44, 2838.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21301) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1804, 3938, 6563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16081) [M::mem_pestat] mean and std.dev: (4274.64, 2831.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20840) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 390.693 CPU sec, 24.940 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5035, 279417, 4543, 4700) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1624, 3907, 6576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16480) [M::mem_pestat] mean and std.dev: (4250.22, 2906.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 257, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (269.37, 105.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1945, 3983, 6560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15790) [M::mem_pestat] mean and std.dev: (4346.68, 2768.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20405) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1691, 3784, 6611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16451) [M::mem_pestat] mean and std.dev: (4249.49, 2881.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21371) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 396.684 CPU sec, 25.335 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4946, 280810, 4480, 4654) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1603, 3681, 6333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15793) [M::mem_pestat] mean and std.dev: (4085.66, 2859.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 257, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (268.54, 104.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1951, 4010, 6649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16045) [M::mem_pestat] mean and std.dev: (4378.33, 2799.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20743) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1755, 3900, 6516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16038) [M::mem_pestat] mean and std.dev: (4230.74, 2830.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20799) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 398.433 CPU sec, 25.370 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4811, 280743, 4437, 4808) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1498, 3693, 6441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16327) [M::mem_pestat] mean and std.dev: (4086.40, 2889.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 256, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (268.49, 104.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1887, 4033, 6587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15987) [M::mem_pestat] mean and std.dev: (4345.91, 2792.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20687) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1745, 3810, 6422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15776) [M::mem_pestat] mean and std.dev: (4195.57, 2804.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20453) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 398.004 CPU sec, 25.392 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4965, 280463, 4436, 4738) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1571, 3690, 6448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16202) [M::mem_pestat] mean and std.dev: (4121.66, 2900.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21079) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 256, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (268.27, 105.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1901, 3999, 6641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16121) [M::mem_pestat] mean and std.dev: (4351.68, 2777.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20861) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1728, 3908, 6443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15873) [M::mem_pestat] mean and std.dev: (4211.20, 2810.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20588) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 397.358 CPU sec, 25.313 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4828, 281267, 4622, 4681) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1623, 3765, 6488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16218) [M::mem_pestat] mean and std.dev: (4139.38, 2886.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21083) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 257, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (269.18, 105.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1912, 4136, 6684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16228) [M::mem_pestat] mean and std.dev: (4402.90, 2805.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21000) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1830, 4032, 6616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16188) [M::mem_pestat] mean and std.dev: (4322.35, 2843.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20974) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 388.504 CPU sec, 24.817 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4795, 280394, 4386, 4887) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1604, 3727, 6454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16154) [M::mem_pestat] mean and std.dev: (4115.47, 2856.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 256, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (268.39, 104.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1882, 3875, 6477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15667) [M::mem_pestat] mean and std.dev: (4268.96, 2773.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20262) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1762, 3920, 6503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15985) [M::mem_pestat] mean and std.dev: (4242.01, 2805.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20726) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 397.306 CPU sec, 25.346 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4978, 279051, 4494, 4825) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1490, 3656, 6406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16238) [M::mem_pestat] mean and std.dev: (4056.08, 2867.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21154) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 256, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (268.44, 104.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1853, 4001, 6632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16190) [M::mem_pestat] mean and std.dev: (4320.65, 2797.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20969) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1780, 3838, 6475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15865) [M::mem_pestat] mean and std.dev: (4237.43, 2807.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20560) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 403.377 CPU sec, 25.611 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4921, 279233, 4383, 4802) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1563, 3816, 6519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16431) [M::mem_pestat] mean and std.dev: (4156.91, 2886.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 257, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (268.84, 104.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1839, 3956, 6713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16461) [M::mem_pestat] mean and std.dev: (4364.92, 2851.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21335) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1838, 3957, 6603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16133) [M::mem_pestat] mean and std.dev: (4303.08, 2831.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20898) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 397.704 CPU sec, 25.437 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4864, 279623, 4585, 4726) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1554, 3670, 6347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15933) [M::mem_pestat] mean and std.dev: (4074.01, 2852.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 257, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (268.94, 105.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1894, 3902, 6408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15436) [M::mem_pestat] mean and std.dev: (4262.02, 2746.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19950) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1725, 3945, 6510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16080) [M::mem_pestat] mean and std.dev: (4242.18, 2833.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20865) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 399.952 CPU sec, 25.552 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4874, 280168, 4515, 4703) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1621, 3707, 6488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16222) [M::mem_pestat] mean and std.dev: (4118.37, 2871.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21089) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 257, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (268.91, 104.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1929, 4071, 6580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15882) [M::mem_pestat] mean and std.dev: (4378.07, 2767.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20533) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1856, 3915, 6511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15821) [M::mem_pestat] mean and std.dev: (4271.98, 2794.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20476) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 388.645 CPU sec, 24.701 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4836, 279668, 4354, 4741) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1492, 3515, 6364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16108) [M::mem_pestat] mean and std.dev: (4032.47, 2897.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 257, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (269.20, 105.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1914, 3955, 6525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15747) [M::mem_pestat] mean and std.dev: (4311.98, 2758.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20358) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1703, 3705, 6369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15701) [M::mem_pestat] mean and std.dev: (4143.92, 2783.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20367) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 388.853 CPU sec, 24.690 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4883, 278764, 4509, 4757) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1552, 3765, 6458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16270) [M::mem_pestat] mean and std.dev: (4122.39, 2880.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21176) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 255, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (266.98, 103.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1982, 4132, 6798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16430) [M::mem_pestat] mean and std.dev: (4463.23, 2807.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21246) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1730, 3856, 6585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16295) [M::mem_pestat] mean and std.dev: (4235.92, 2818.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21150) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 422.073 CPU sec, 26.825 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4838, 278576, 4501, 4747) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1584, 3752, 6459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16209) [M::mem_pestat] mean and std.dev: (4127.44, 2875.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21084) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 257, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (268.60, 104.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1919, 3967, 6603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15971) [M::mem_pestat] mean and std.dev: (4349.86, 2794.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20655) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1742, 3782, 6321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15479) [M::mem_pestat] mean and std.dev: (4158.39, 2789.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20058) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 403.117 CPU sec, 25.683 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4926, 279241, 4394, 4818) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1527, 3669, 6425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16221) [M::mem_pestat] mean and std.dev: (4061.90, 2855.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21119) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 256, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (268.02, 104.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 779) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1906, 3909, 6505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15703) [M::mem_pestat] mean and std.dev: (4292.96, 2758.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20302) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1758, 3911, 6520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16044) [M::mem_pestat] mean and std.dev: (4245.78, 2825.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20806) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 400.217 CPU sec, 25.480 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4954, 279715, 4445, 4732) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1536, 3761, 6412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16164) [M::mem_pestat] mean and std.dev: (4102.18, 2865.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21040) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 256, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (267.68, 104.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1918, 4080, 6803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16573) [M::mem_pestat] mean and std.dev: (4417.48, 2833.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21458) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1683, 3901, 6381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15777) [M::mem_pestat] mean and std.dev: (4180.88, 2807.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20475) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 399.620 CPU sec, 25.544 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4837, 279436, 4539, 4840) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1518, 3773, 6470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16374) [M::mem_pestat] mean and std.dev: (4089.00, 2892.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21326) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 256, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (267.42, 103.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1872, 3987, 6618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16110) [M::mem_pestat] mean and std.dev: (4336.30, 2797.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20856) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1849, 4014, 6600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16102) [M::mem_pestat] mean and std.dev: (4333.33, 2838.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20853) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 399.985 CPU sec, 25.577 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4896, 279786, 4422, 4849) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1559, 3828, 6475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16307) [M::mem_pestat] mean and std.dev: (4131.36, 2871.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21223) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 255, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (267.46, 103.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1839, 4068, 6751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16575) [M::mem_pestat] mean and std.dev: (4375.10, 2811.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21487) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1799, 3971, 6694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16484) [M::mem_pestat] mean and std.dev: (4287.55, 2867.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21379) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 399.146 CPU sec, 25.432 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5002, 280561, 4391, 4740) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1619, 3688, 6471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16175) [M::mem_pestat] mean and std.dev: (4118.54, 2886.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 255, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (266.80, 103.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1933, 4032, 6649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16081) [M::mem_pestat] mean and std.dev: (4366.76, 2777.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20797) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1687, 3718, 6408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15850) [M::mem_pestat] mean and std.dev: (4155.34, 2814.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20571) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 406.440 CPU sec, 25.976 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4869, 279294, 4504, 4696) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1436, 3683, 6363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16217) [M::mem_pestat] mean and std.dev: (4039.21, 2895.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21144) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 255, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (267.22, 103.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1972, 4115, 6713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16195) [M::mem_pestat] mean and std.dev: (4426.25, 2779.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20936) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1715, 3988, 6614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16412) [M::mem_pestat] mean and std.dev: (4267.03, 2853.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21311) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 400.846 CPU sec, 25.523 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4939, 279328, 4281, 4746) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1478, 3619, 6290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15914) [M::mem_pestat] mean and std.dev: (4010.87, 2879.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 256, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (267.36, 103.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1943, 4018, 6581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15857) [M::mem_pestat] mean and std.dev: (4364.46, 2781.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20495) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1669, 3866, 6475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16087) [M::mem_pestat] mean and std.dev: (4216.16, 2869.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20893) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 410.316 CPU sec, 26.172 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4930, 280086, 4332, 4753) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1535, 3772, 6464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16322) [M::mem_pestat] mean and std.dev: (4112.94, 2893.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21251) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 255, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (267.04, 103.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1890, 4011, 6637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16131) [M::mem_pestat] mean and std.dev: (4347.21, 2779.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20878) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1683, 3817, 6445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15969) [M::mem_pestat] mean and std.dev: (4151.30, 2812.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20731) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 405.425 CPU sec, 25.799 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4877, 278307, 4431, 4671) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1455, 3597, 6333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16089) [M::mem_pestat] mean and std.dev: (4014.81, 2875.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 254, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (265.79, 102.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1868, 3928, 6648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16208) [M::mem_pestat] mean and std.dev: (4315.28, 2819.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20988) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1746, 3860, 6418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15762) [M::mem_pestat] mean and std.dev: (4207.31, 2808.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20434) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 427.636 CPU sec, 27.182 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4941, 279261, 4333, 4639) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1458, 3730, 6482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16530) [M::mem_pestat] mean and std.dev: (4094.05, 2943.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 254, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (265.70, 102.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1884, 3955, 6599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16029) [M::mem_pestat] mean and std.dev: (4328.85, 2792.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20744) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1691, 3778, 6418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15872) [M::mem_pestat] mean and std.dev: (4185.78, 2840.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20599) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 431.491 CPU sec, 27.738 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4942, 278834, 4338, 4817) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1584, 3809, 6488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16296) [M::mem_pestat] mean and std.dev: (4136.47, 2898.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21200) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 254, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (266.09, 102.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1879, 3995, 6619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16099) [M::mem_pestat] mean and std.dev: (4360.91, 2793.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20839) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1833, 3878, 6581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16077) [M::mem_pestat] mean and std.dev: (4260.51, 2799.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20825) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 408.647 CPU sec, 26.200 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4905, 278777, 4230, 4753) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1399, 3533, 6392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16378) [M::mem_pestat] mean and std.dev: (3989.41, 2907.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21371) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 254, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (265.68, 102.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1939, 4010, 6680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16162) [M::mem_pestat] mean and std.dev: (4385.74, 2801.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20903) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1737, 3904, 6489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15993) [M::mem_pestat] mean and std.dev: (4240.05, 2833.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20745) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 416.744 CPU sec, 26.518 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5041, 279754, 4465, 4762) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1504, 3667, 6328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15976) [M::mem_pestat] mean and std.dev: (4021.66, 2883.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 253, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (264.77, 101.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1901, 4022, 6709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16325) [M::mem_pestat] mean and std.dev: (4392.92, 2831.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21133) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1740, 3891, 6512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16056) [M::mem_pestat] mean and std.dev: (4227.88, 2824.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20828) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 414.031 CPU sec, 26.401 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5002, 282052, 4433, 4719) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1437, 3439, 6181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15669) [M::mem_pestat] mean and std.dev: (3921.16, 2857.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (261.84, 99.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1958, 4111, 6665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16079) [M::mem_pestat] mean and std.dev: (4410.15, 2780.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20786) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1768, 3828, 6385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15619) [M::mem_pestat] mean and std.dev: (4208.51, 2812.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20236) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 418.436 CPU sec, 26.635 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4836, 289413, 4502, 4610) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1579, 3825, 6628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16726) [M::mem_pestat] mean and std.dev: (4216.49, 2927.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 247, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (258.00, 96.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (2011, 4235, 6688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16042) [M::mem_pestat] mean and std.dev: (4477.15, 2801.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20719) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1727, 3822, 6394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15728) [M::mem_pestat] mean and std.dev: (4194.89, 2803.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20395) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 389.568 CPU sec, 24.801 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4942, 288370, 4153, 4772) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1646, 3998, 6645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16643) [M::mem_pestat] mean and std.dev: (4262.89, 2888.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 248, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (258.27, 96.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1931, 4136, 6513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15677) [M::mem_pestat] mean and std.dev: (4369.33, 2750.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20259) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1733, 3794, 6418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15788) [M::mem_pestat] mean and std.dev: (4205.16, 2836.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20473) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 413.315 CPU sec, 26.327 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4638, 288611, 4340, 4591) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1616, 3767, 6303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15677) [M::mem_pestat] mean and std.dev: (4119.70, 2857.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 249, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (259.35, 97.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1877, 3988, 6730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16436) [M::mem_pestat] mean and std.dev: (4352.76, 2809.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21289) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1720, 3927, 6595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16345) [M::mem_pestat] mean and std.dev: (4269.69, 2847.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21220) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 443.173 CPU sec, 28.654 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4740, 290054, 4109, 4835) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1628, 3635, 6332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15740) [M::mem_pestat] mean and std.dev: (4061.40, 2830.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 248, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (258.04, 96.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1826, 3858, 6735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16553) [M::mem_pestat] mean and std.dev: (4339.37, 2841.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21462) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1701, 3694, 6531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16191) [M::mem_pestat] mean and std.dev: (4185.37, 2855.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21021) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 383.849 CPU sec, 24.581 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4737, 289025, 4364, 4708) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1624, 3735, 6488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16216) [M::mem_pestat] mean and std.dev: (4155.77, 2865.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21080) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 248, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (258.50, 97.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1904, 3972, 6563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15881) [M::mem_pestat] mean and std.dev: (4347.56, 2806.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20540) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1714, 3888, 6312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15508) [M::mem_pestat] mean and std.dev: (4187.64, 2812.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20106) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 403.309 CPU sec, 25.718 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4649, 288047, 4311, 4954) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1761, 3820, 6449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15825) [M::mem_pestat] mean and std.dev: (4171.46, 2799.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 248, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (258.91, 97.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1847, 4095, 6762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16592) [M::mem_pestat] mean and std.dev: (4396.78, 2840.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21507) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1681, 3694, 6360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15718) [M::mem_pestat] mean and std.dev: (4113.95, 2795.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20397) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 394.000 CPU sec, 25.183 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4673, 288782, 4389, 4677) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1581, 3870, 6723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17007) [M::mem_pestat] mean and std.dev: (4239.35, 2938.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22149) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 247, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (258.41, 97.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1949, 4095, 6694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16184) [M::mem_pestat] mean and std.dev: (4419.64, 2825.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20929) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1738, 3914, 6586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16282) [M::mem_pestat] mean and std.dev: (4240.73, 2859.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21130) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 412.434 CPU sec, 26.287 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4694, 288310, 4426, 4686) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1724, 3818, 6514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16094) [M::mem_pestat] mean and std.dev: (4182.93, 2841.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 248, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (258.33, 96.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1935, 4044, 6660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16110) [M::mem_pestat] mean and std.dev: (4399.08, 2773.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20835) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1878, 3945, 6651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16197) [M::mem_pestat] mean and std.dev: (4301.23, 2836.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20970) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 465.590 CPU sec, 29.561 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4576, 288402, 4110, 4601) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1582, 3822, 6646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16774) [M::mem_pestat] mean and std.dev: (4179.75, 2915.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 247, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (258.04, 97.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1931, 4089, 6774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16460) [M::mem_pestat] mean and std.dev: (4431.50, 2830.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21303) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1796, 3724, 6443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15737) [M::mem_pestat] mean and std.dev: (4197.25, 2821.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20384) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 545.798 CPU sec, 34.638 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4945, 288089, 4352, 4605) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1490, 3704, 6440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16340) [M::mem_pestat] mean and std.dev: (4072.55, 2864.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21290) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 246, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (257.43, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1963, 4086, 6646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16012) [M::mem_pestat] mean and std.dev: (4394.95, 2783.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20695) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1748, 3819, 6516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16052) [M::mem_pestat] mean and std.dev: (4241.86, 2897.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20820) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 399.522 CPU sec, 25.476 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4704, 289467, 4371, 4800) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1667, 3727, 6416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15914) [M::mem_pestat] mean and std.dev: (4162.69, 2869.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 245, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (255.62, 95.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1873, 3924, 6627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16135) [M::mem_pestat] mean and std.dev: (4307.82, 2799.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20889) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1753, 3889, 6454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15856) [M::mem_pestat] mean and std.dev: (4221.15, 2825.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20557) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 421.657 CPU sec, 26.946 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4811, 290799, 4047, 4649) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1566, 3569, 6297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15759) [M::mem_pestat] mean and std.dev: (4049.05, 2844.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 243, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (254.28, 95.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 719) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1934, 4175, 6743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16361) [M::mem_pestat] mean and std.dev: (4447.00, 2820.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21170) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1908, 4007, 6629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16071) [M::mem_pestat] mean and std.dev: (4316.09, 2811.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20792) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 422.231 CPU sec, 26.840 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4737, 284684, 4498, 4539) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1549, 3717, 6494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16384) [M::mem_pestat] mean and std.dev: (4113.13, 2923.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21329) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 247, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (257.39, 96.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 729) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1954, 4100, 6725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16267) [M::mem_pestat] mean and std.dev: (4444.19, 2796.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21038) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1665, 3634, 6341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15693) [M::mem_pestat] mean and std.dev: (4130.19, 2816.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20369) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 505.448 CPU sec, 32.121 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4736, 287933, 4286, 4705) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1594, 3831, 6470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16222) [M::mem_pestat] mean and std.dev: (4159.06, 2856.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21098) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 247, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (258.17, 97.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1935, 4053, 6682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16176) [M::mem_pestat] mean and std.dev: (4399.33, 2788.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20923) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1859, 3871, 6585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16037) [M::mem_pestat] mean and std.dev: (4295.15, 2824.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20763) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 446.201 CPU sec, 28.461 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4715, 290813, 4112, 4597) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1711, 3742, 6447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15919) [M::mem_pestat] mean and std.dev: (4137.61, 2830.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 246, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (256.88, 96.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1968, 4113, 6775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16389) [M::mem_pestat] mean and std.dev: (4437.89, 2827.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21196) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1673, 3786, 6448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15998) [M::mem_pestat] mean and std.dev: (4206.22, 2823.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 428.924 CPU sec, 27.306 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4734, 289967, 4338, 4686) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1598, 3822, 6504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16316) [M::mem_pestat] mean and std.dev: (4200.00, 2917.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21222) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 246, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (256.97, 96.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1818, 3828, 6563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16053) [M::mem_pestat] mean and std.dev: (4278.67, 2815.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20798) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1789, 3928, 6345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15457) [M::mem_pestat] mean and std.dev: (4224.82, 2802.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20013) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 383.900 CPU sec, 24.513 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4539, 288999, 4148, 4593) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1560, 3663, 6414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16122) [M::mem_pestat] mean and std.dev: (4097.22, 2868.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20976) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 245, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (255.85, 96.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1864, 3934, 6569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15979) [M::mem_pestat] mean and std.dev: (4307.91, 2783.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20684) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1694, 3873, 6440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15932) [M::mem_pestat] mean and std.dev: (4208.51, 2829.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20678) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 380.506 CPU sec, 24.302 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4663, 291914, 4229, 4392) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1735, 3752, 6544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16162) [M::mem_pestat] mean and std.dev: (4176.24, 2851.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20971) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 243, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (253.29, 95.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1891, 3922, 6579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15955) [M::mem_pestat] mean and std.dev: (4319.35, 2786.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20643) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1808, 3918, 6683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16433) [M::mem_pestat] mean and std.dev: (4307.45, 2835.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21308) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 417.318 CPU sec, 26.682 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4833, 287290, 4399, 4611) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1669, 3831, 6391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15835) [M::mem_pestat] mean and std.dev: (4168.24, 2850.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 243, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (254.37, 96.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1884, 4023, 6740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16452) [M::mem_pestat] mean and std.dev: (4389.78, 2818.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21308) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1907, 3957, 6642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16112) [M::mem_pestat] mean and std.dev: (4342.47, 2827.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20847) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 486.836 CPU sec, 30.855 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4561, 288270, 4282, 4356) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1579, 3720, 6459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16219) [M::mem_pestat] mean and std.dev: (4146.36, 2891.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21099) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 245, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (255.33, 95.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1922, 4012, 6515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15701) [M::mem_pestat] mean and std.dev: (4340.03, 2798.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20294) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1807, 3901, 6541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16009) [M::mem_pestat] mean and std.dev: (4259.36, 2839.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20743) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 423.881 CPU sec, 26.920 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4579, 287741, 4380, 4766) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1705, 3762, 6452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15946) [M::mem_pestat] mean and std.dev: (4175.32, 2863.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 244, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (255.46, 96.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1828, 3936, 6563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16033) [M::mem_pestat] mean and std.dev: (4280.10, 2778.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20768) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1785, 3716, 6443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15759) [M::mem_pestat] mean and std.dev: (4178.71, 2813.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20417) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 392.539 CPU sec, 25.124 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4818, 287964, 4237, 4461) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1584, 3595, 6267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15633) [M::mem_pestat] mean and std.dev: (4027.93, 2826.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20316) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 244, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (255.29, 95.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1976, 4098, 6648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15992) [M::mem_pestat] mean and std.dev: (4395.56, 2758.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20664) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1774, 4011, 6552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16108) [M::mem_pestat] mean and std.dev: (4286.25, 2824.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20886) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 410.808 CPU sec, 26.236 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4624, 288693, 4290, 4688) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1800, 3822, 6540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16020) [M::mem_pestat] mean and std.dev: (4233.06, 2852.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 246, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (257.31, 97.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1837, 4025, 6612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16162) [M::mem_pestat] mean and std.dev: (4318.02, 2782.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20937) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1724, 3803, 6429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15839) [M::mem_pestat] mean and std.dev: (4196.07, 2805.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20544) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 500.302 CPU sec, 31.766 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4739, 287790, 4280, 4600) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1615, 3767, 6371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15883) [M::mem_pestat] mean and std.dev: (4100.97, 2845.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 245, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (256.56, 97.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1930, 3973, 6527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15721) [M::mem_pestat] mean and std.dev: (4326.31, 2755.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20318) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1788, 3891, 6445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15759) [M::mem_pestat] mean and std.dev: (4195.94, 2790.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20416) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 501.026 CPU sec, 31.925 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4800, 283133, 4363, 4587) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1600, 3723, 6468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16204) [M::mem_pestat] mean and std.dev: (4143.52, 2898.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21072) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 245, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (255.77, 95.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1870, 4001, 6714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16402) [M::mem_pestat] mean and std.dev: (4388.21, 2828.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21246) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1763, 3726, 6430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15764) [M::mem_pestat] mean and std.dev: (4205.21, 2852.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20431) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 487.013 CPU sec, 30.927 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4622, 285937, 4359, 4588) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1653, 3831, 6401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15897) [M::mem_pestat] mean and std.dev: (4154.42, 2851.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 246, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (257.15, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1835, 3913, 6766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16628) [M::mem_pestat] mean and std.dev: (4329.31, 2829.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21559) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1794, 3895, 6522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15978) [M::mem_pestat] mean and std.dev: (4263.20, 2846.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20706) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 410.607 CPU sec, 26.134 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4658, 285890, 4360, 4603) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1633, 3740, 6310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15664) [M::mem_pestat] mean and std.dev: (4112.64, 2833.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20341) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 245, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (256.62, 97.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1785, 3911, 6572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16146) [M::mem_pestat] mean and std.dev: (4288.03, 2820.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20933) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1703, 3863, 6450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15944) [M::mem_pestat] mean and std.dev: (4211.37, 2842.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20691) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 418.447 CPU sec, 26.665 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4682, 283783, 4398, 4576) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1613, 3726, 6346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15812) [M::mem_pestat] mean and std.dev: (4102.26, 2858.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 246, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (257.37, 96.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1931, 3988, 6579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15875) [M::mem_pestat] mean and std.dev: (4375.69, 2806.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20523) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1765, 3778, 6554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16132) [M::mem_pestat] mean and std.dev: (4243.45, 2853.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20921) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 405.522 CPU sec, 25.781 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4711, 281723, 4193, 4701) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1539, 3757, 6373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16041) [M::mem_pestat] mean and std.dev: (4095.20, 2871.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 246, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (257.63, 96.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1886, 4036, 6545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15863) [M::mem_pestat] mean and std.dev: (4349.80, 2785.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20522) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1704, 3643, 6359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15669) [M::mem_pestat] mean and std.dev: (4139.24, 2806.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20324) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 427.686 CPU sec, 27.272 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4793, 283326, 4414, 4711) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1551, 3659, 6401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16101) [M::mem_pestat] mean and std.dev: (4079.17, 2844.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 253, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (264.81, 103.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1861, 3985, 6614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16120) [M::mem_pestat] mean and std.dev: (4325.80, 2803.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20873) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1702, 3832, 6519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16153) [M::mem_pestat] mean and std.dev: (4199.05, 2838.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20970) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 389.390 CPU sec, 24.884 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4828, 284228, 4494, 4761) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1566, 3645, 6292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15744) [M::mem_pestat] mean and std.dev: (4053.67, 2817.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 253, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (265.08, 103.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1932, 3960, 6611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15969) [M::mem_pestat] mean and std.dev: (4350.63, 2763.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20648) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1729, 3884, 6558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16216) [M::mem_pestat] mean and std.dev: (4263.14, 2845.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21045) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 374.113 CPU sec, 23.865 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4743, 284369, 4383, 4798) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1685, 3764, 6550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16280) [M::mem_pestat] mean and std.dev: (4197.38, 2864.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21145) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 254, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (266.23, 104.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1945, 4067, 6663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16099) [M::mem_pestat] mean and std.dev: (4398.12, 2787.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20817) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1804, 3863, 6548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16036) [M::mem_pestat] mean and std.dev: (4259.98, 2846.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20780) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 367.715 CPU sec, 23.513 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4912, 285448, 4382, 4742) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1573, 3762, 6468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16258) [M::mem_pestat] mean and std.dev: (4136.16, 2839.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21153) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 253, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (264.81, 102.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1934, 4101, 6775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16457) [M::mem_pestat] mean and std.dev: (4435.34, 2822.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21298) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1700, 3889, 6462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15986) [M::mem_pestat] mean and std.dev: (4199.83, 2836.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20748) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 366.965 CPU sec, 23.386 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4847, 285349, 4414, 4609) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1633, 3724, 6346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15772) [M::mem_pestat] mean and std.dev: (4098.68, 2832.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 253, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (264.29, 102.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1966, 4012, 6627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15949) [M::mem_pestat] mean and std.dev: (4383.48, 2786.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20610) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1684, 3942, 6579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16369) [M::mem_pestat] mean and std.dev: (4257.31, 2855.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21264) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 368.891 CPU sec, 23.534 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4742, 285458, 4394, 4622) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1405, 3435, 6278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16024) [M::mem_pestat] mean and std.dev: (3932.54, 2843.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20897) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 252, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (263.99, 102.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (2026, 4110, 6709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16075) [M::mem_pestat] mean and std.dev: (4465.07, 2787.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20758) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1776, 3890, 6461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15831) [M::mem_pestat] mean and std.dev: (4220.61, 2811.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20516) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 370.243 CPU sec, 23.598 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4659, 285766, 4408, 4712) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1532, 3640, 6504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16448) [M::mem_pestat] mean and std.dev: (4104.92, 2901.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 251, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (263.26, 102.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1958, 4006, 6672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16100) [M::mem_pestat] mean and std.dev: (4402.85, 2807.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20814) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1704, 3816, 6452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15948) [M::mem_pestat] mean and std.dev: (4188.48, 2825.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20696) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 371.557 CPU sec, 23.738 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4860, 285749, 4384, 4782) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1644, 3830, 6514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16254) [M::mem_pestat] mean and std.dev: (4168.18, 2852.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21124) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 251, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (262.83, 101.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1852, 4089, 6730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16486) [M::mem_pestat] mean and std.dev: (4385.73, 2837.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21364) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1725, 3895, 6399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15747) [M::mem_pestat] mean and std.dev: (4194.43, 2808.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20421) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 372.056 CPU sec, 24.044 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4789, 286854, 4298, 4694) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1612, 3831, 6488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16240) [M::mem_pestat] mean and std.dev: (4170.04, 2865.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21116) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 250, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (261.79, 101.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1811, 3904, 6485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15833) [M::mem_pestat] mean and std.dev: (4291.24, 2812.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20507) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1776, 3924, 6426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15726) [M::mem_pestat] mean and std.dev: (4213.22, 2790.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20376) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 368.346 CPU sec, 23.523 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4861, 287204, 4388, 4688) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] (25, 50, 75) percentile: (1629, 3673, 6401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15945) [M::mem_pestat] mean and std.dev: (4104.50, 2855.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 250, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (261.54, 100.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1842, 3884, 6624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16188) [M::mem_pestat] mean and std.dev: (4304.47, 2815.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20970) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1811, 3939, 6382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15524) [M::mem_pestat] mean and std.dev: (4258.67, 2783.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 370.661 CPU sec, 23.512 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4789, 286853, 4392, 4660) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1649, 3831, 6527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16283) [M::mem_pestat] mean and std.dev: (4202.58, 2873.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 250, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (261.01, 100.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 754) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1927, 4095, 6743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16375) [M::mem_pestat] mean and std.dev: (4426.08, 2829.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21191) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1761, 3795, 6396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15666) [M::mem_pestat] mean and std.dev: (4177.29, 2806.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20301) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 375.966 CPU sec, 24.092 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4675, 287306, 4498, 4725) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1561, 3623, 6335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15883) [M::mem_pestat] mean and std.dev: (4055.12, 2856.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 248, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (259.61, 99.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1941, 3937, 6582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15864) [M::mem_pestat] mean and std.dev: (4337.76, 2765.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20505) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1703, 3853, 6373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15713) [M::mem_pestat] mean and std.dev: (4180.21, 2804.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20383) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 369.411 CPU sec, 23.589 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4755, 287482, 4264, 4793) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1604, 3787, 6542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16418) [M::mem_pestat] mean and std.dev: (4160.05, 2893.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 249, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (259.90, 99.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1819, 3881, 6468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15766) [M::mem_pestat] mean and std.dev: (4262.70, 2787.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20415) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1668, 3728, 6405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15879) [M::mem_pestat] mean and std.dev: (4160.42, 2839.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20616) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 368.288 CPU sec, 23.551 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4757, 285991, 4410, 4612) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1573, 3831, 6586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16612) [M::mem_pestat] mean and std.dev: (4186.71, 2904.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 250, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (260.89, 99.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (2024, 4017, 6660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15932) [M::mem_pestat] mean and std.dev: (4395.13, 2763.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20568) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1700, 3856, 6445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15935) [M::mem_pestat] mean and std.dev: (4204.19, 2821.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20680) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 375.289 CPU sec, 23.917 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4779, 287337, 4330, 4666) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1739, 3711, 6451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15875) [M::mem_pestat] mean and std.dev: (4151.98, 2830.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 249, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (259.94, 99.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1846, 4007, 6730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16498) [M::mem_pestat] mean and std.dev: (4371.34, 2816.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1813, 3849, 6449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15721) [M::mem_pestat] mean and std.dev: (4261.64, 2819.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20357) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 372.495 CPU sec, 23.771 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4749, 285798, 4278, 4638) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1551, 3737, 6541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16521) [M::mem_pestat] mean and std.dev: (4119.52, 2897.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 249, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (260.38, 99.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1875, 3965, 6572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15966) [M::mem_pestat] mean and std.dev: (4348.79, 2800.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20663) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1649, 3680, 6450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16052) [M::mem_pestat] mean and std.dev: (4145.36, 2820.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20853) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 384.822 CPU sec, 24.611 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4875, 287251, 4239, 4713) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1553, 3717, 6419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16151) [M::mem_pestat] mean and std.dev: (4118.05, 2864.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21017) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 249, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (260.33, 99.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1927, 4047, 6654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16108) [M::mem_pestat] mean and std.dev: (4376.11, 2800.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20835) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1789, 4036, 6569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16129) [M::mem_pestat] mean and std.dev: (4303.92, 2834.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20909) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 370.266 CPU sec, 23.560 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4863, 287310, 4336, 4564) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1623, 3756, 6488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16218) [M::mem_pestat] mean and std.dev: (4162.10, 2879.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21083) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 248, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (259.48, 99.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1985, 4216, 6818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16484) [M::mem_pestat] mean and std.dev: (4471.71, 2788.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21317) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1858, 3973, 6528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15868) [M::mem_pestat] mean and std.dev: (4315.65, 2833.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20538) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 367.120 CPU sec, 23.492 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4677, 286911, 4294, 4608) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1668, 3829, 6420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15924) [M::mem_pestat] mean and std.dev: (4150.26, 2817.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 248, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (259.49, 99.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1984, 4039, 6716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16180) [M::mem_pestat] mean and std.dev: (4426.99, 2787.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20912) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1718, 3969, 6456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15932) [M::mem_pestat] mean and std.dev: (4227.76, 2827.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20670) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 372.247 CPU sec, 23.829 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4742, 286323, 4305, 4684) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1577, 3719, 6453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16205) [M::mem_pestat] mean and std.dev: (4123.16, 2868.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 248, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (259.59, 99.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1913, 4036, 6620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16034) [M::mem_pestat] mean and std.dev: (4352.49, 2789.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20741) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1810, 4001, 6508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15904) [M::mem_pestat] mean and std.dev: (4276.92, 2814.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20602) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 371.246 CPU sec, 23.747 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4697, 286589, 4386, 4702) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1584, 3700, 6467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16233) [M::mem_pestat] mean and std.dev: (4105.38, 2858.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21116) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 248, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (259.43, 99.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1932, 4012, 6704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16248) [M::mem_pestat] mean and std.dev: (4382.10, 2818.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21020) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1834, 3861, 6452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15688) [M::mem_pestat] mean and std.dev: (4234.87, 2790.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20306) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 371.537 CPU sec, 23.798 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4796, 285407, 4241, 4568) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1541, 3650, 6357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15989) [M::mem_pestat] mean and std.dev: (4048.24, 2845.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20805) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 249, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (259.93, 99.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1933, 4136, 6671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16147) [M::mem_pestat] mean and std.dev: (4423.06, 2784.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20885) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1668, 3857, 6416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15912) [M::mem_pestat] mean and std.dev: (4180.32, 2837.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20660) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 376.937 CPU sec, 23.961 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4818, 286433, 4331, 4697) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1633, 3926, 6488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16198) [M::mem_pestat] mean and std.dev: (4197.28, 2860.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21053) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 248, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (259.53, 99.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1935, 4068, 6735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16335) [M::mem_pestat] mean and std.dev: (4407.83, 2803.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21135) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1795, 3915, 6521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15973) [M::mem_pestat] mean and std.dev: (4243.05, 2820.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20699) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 377.869 CPU sec, 24.104 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4688, 286334, 4436, 4645) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1565, 3693, 6514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16412) [M::mem_pestat] mean and std.dev: (4109.21, 2883.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 249, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (260.03, 99.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1931, 4041, 6606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15956) [M::mem_pestat] mean and std.dev: (4383.14, 2785.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20631) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1809, 3880, 6550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16032) [M::mem_pestat] mean and std.dev: (4244.82, 2833.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 376.487 CPU sec, 24.067 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4678, 285543, 4219, 4605) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1611, 3723, 6423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16047) [M::mem_pestat] mean and std.dev: (4127.97, 2863.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 248, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (259.02, 98.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1894, 4002, 6529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15799) [M::mem_pestat] mean and std.dev: (4339.87, 2782.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20434) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1781, 3888, 6548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16082) [M::mem_pestat] mean and std.dev: (4244.57, 2830.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20849) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 387.492 CPU sec, 24.755 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5008, 286064, 4364, 4685) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1552, 3657, 6483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16345) [M::mem_pestat] mean and std.dev: (4094.24, 2909.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21276) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 248, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (259.10, 99.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1940, 4069, 6746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16358) [M::mem_pestat] mean and std.dev: (4416.64, 2817.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21164) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1851, 3926, 6436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15606) [M::mem_pestat] mean and std.dev: (4250.04, 2783.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 392.260 CPU sec, 24.986 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4841, 287237, 4254, 4596) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1540, 3726, 6458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16294) [M::mem_pestat] mean and std.dev: (4114.36, 2890.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21212) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 248, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (259.42, 99.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1838, 3939, 6660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16304) [M::mem_pestat] mean and std.dev: (4321.37, 2825.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21126) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1799, 3908, 6583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16151) [M::mem_pestat] mean and std.dev: (4285.44, 2866.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20935) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 373.222 CPU sec, 23.869 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4891, 285771, 4251, 4666) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1582, 3705, 6346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15874) [M::mem_pestat] mean and std.dev: (4093.26, 2866.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 248, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (259.26, 99.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1833, 3955, 6637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16245) [M::mem_pestat] mean and std.dev: (4319.81, 2818.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21049) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1681, 3760, 6409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15865) [M::mem_pestat] mean and std.dev: (4160.41, 2818.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20593) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 383.713 CPU sec, 24.539 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4594, 286981, 4257, 4512) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1541, 3619, 6233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15617) [M::mem_pestat] mean and std.dev: (4031.12, 2830.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 247, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (257.63, 97.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1951, 4085, 6795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16483) [M::mem_pestat] mean and std.dev: (4439.73, 2799.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1712, 3814, 6473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15995) [M::mem_pestat] mean and std.dev: (4192.11, 2824.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20756) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 375.411 CPU sec, 23.952 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5760, 225213, 5211, 5602) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1537, 3698, 6454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16288) [M::mem_pestat] mean and std.dev: (4112.70, 2867.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21205) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 261, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (274.22, 109.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1872, 4027, 6787) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16617) [M::mem_pestat] mean and std.dev: (4388.93, 2831.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21532) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1722, 3832, 6445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15891) [M::mem_pestat] mean and std.dev: (4219.98, 2823.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20614) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 411.336 CPU sec, 26.140 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7288, 128679, 6474, 7176) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1562, 3694, 6417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16127) [M::mem_pestat] mean and std.dev: (4099.93, 2835.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (239, 322, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (324.31, 123.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1861, 3884, 6477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15709) [M::mem_pestat] mean and std.dev: (4257.48, 2770.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20325) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1666, 3678, 6379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15805) [M::mem_pestat] mean and std.dev: (4149.36, 2803.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20518) [M::mem_process_seqs] Processed 1333334 reads in 474.538 CPU sec, 32.421 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7435, 131716, 6739, 7320) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1588, 3591, 6286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15682) [M::mem_pestat] mean and std.dev: (4060.09, 2810.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (240, 323, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (325.48, 123.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1793, 3865, 6462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15800) [M::mem_pestat] mean and std.dev: (4239.14, 2797.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20469) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1638, 3655, 6353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15783) [M::mem_pestat] mean and std.dev: (4124.20, 2808.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20498) [M::mem_process_seqs] Processed 1333334 reads in 422.555 CPU sec, 27.502 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7298, 131053, 6609, 7368) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1570, 3654, 6392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16036) [M::mem_pestat] mean and std.dev: (4124.86, 2858.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 325, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (327.12, 123.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1822, 3859, 6479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15793) [M::mem_pestat] mean and std.dev: (4260.45, 2800.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20450) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1662, 3688, 6379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15813) [M::mem_pestat] mean and std.dev: (4147.81, 2816.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20530) [M::mem_process_seqs] Processed 1333334 reads in 420.816 CPU sec, 27.187 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7307, 130411, 6657, 7103) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1565, 3613, 6254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15632) [M::mem_pestat] mean and std.dev: (4081.80, 2820.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (240, 323, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (325.56, 123.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1856, 3948, 6570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15998) [M::mem_pestat] mean and std.dev: (4290.08, 2793.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20712) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1633, 3655, 6355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15799) [M::mem_pestat] mean and std.dev: (4122.39, 2807.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20521) [M::mem_process_seqs] Processed 1333334 reads in 430.709 CPU sec, 27.434 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7270, 130420, 6794, 7060) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1559, 3590, 6321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15845) [M::mem_pestat] mean and std.dev: (4078.03, 2831.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (241, 324, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (325.89, 123.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1792, 3860, 6491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15889) [M::mem_pestat] mean and std.dev: (4251.14, 2812.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20588) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1632, 3691, 6407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15957) [M::mem_pestat] mean and std.dev: (4154.36, 2826.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20732) [M::mem_process_seqs] Processed 1333334 reads in 419.326 CPU sec, 27.130 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7214, 131243, 6721, 7148) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1665, 3607, 6218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15324) [M::mem_pestat] mean and std.dev: (4081.94, 2791.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (241, 325, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (326.94, 123.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1855, 3889, 6604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16102) [M::mem_pestat] mean and std.dev: (4297.35, 2820.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20851) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1634, 3652, 6491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16205) [M::mem_pestat] mean and std.dev: (4163.41, 2867.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21062) [M::mem_process_seqs] Processed 1333334 reads in 420.108 CPU sec, 26.735 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7291, 126721, 6520, 6998) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1584, 3673, 6352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15888) [M::mem_pestat] mean and std.dev: (4107.74, 2834.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (240, 323, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (325.39, 123.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1838, 3891, 6618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16178) [M::mem_pestat] mean and std.dev: (4281.21, 2803.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20958) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1629, 3683, 6296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15630) [M::mem_pestat] mean and std.dev: (4115.23, 2805.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20297) [M::mem_process_seqs] Processed 1333334 reads in 480.013 CPU sec, 31.325 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7278, 129848, 6743, 7038) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1554, 3674, 6371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16005) [M::mem_pestat] mean and std.dev: (4079.72, 2830.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 327, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (329.25, 124.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1861, 3832, 6399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15475) [M::mem_pestat] mean and std.dev: (4236.65, 2771.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20013) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1642, 3655, 6300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15616) [M::mem_pestat] mean and std.dev: (4120.12, 2815.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20274) [M::mem_process_seqs] Processed 1333334 reads in 415.600 CPU sec, 26.442 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7131, 129134, 6815, 7050) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1588, 3638, 6296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15712) [M::mem_pestat] mean and std.dev: (4083.12, 2818.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 327, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (329.46, 124.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1809, 3827, 6376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15510) [M::mem_pestat] mean and std.dev: (4201.45, 2785.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20077) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1688, 3731, 6385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15779) [M::mem_pestat] mean and std.dev: (4153.67, 2809.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20476) [M::mem_process_seqs] Processed 1333334 reads in 416.213 CPU sec, 26.401 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7447, 127229, 6454, 6953) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1637, 3692, 6458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16100) [M::mem_pestat] mean and std.dev: (4157.40, 2829.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 325, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (328.05, 123.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1752, 3799, 6496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15984) [M::mem_pestat] mean and std.dev: (4237.38, 2804.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20728) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1684, 3631, 6235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15337) [M::mem_pestat] mean and std.dev: (4103.23, 2809.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19888) [M::mem_process_seqs] Processed 1333334 reads in 459.153 CPU sec, 29.569 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7319, 129743, 6535, 7158) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1556, 3601, 6372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16004) [M::mem_pestat] mean and std.dev: (4082.72, 2837.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20820) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 328, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (330.27, 125.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1921, 3924, 6527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15739) [M::mem_pestat] mean and std.dev: (4315.60, 2780.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20345) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1686, 3676, 6326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15606) [M::mem_pestat] mean and std.dev: (4138.27, 2790.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20246) [M::mem_process_seqs] Processed 1333334 reads in 410.147 CPU sec, 26.214 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7358, 128930, 6576, 7257) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1589, 3584, 6358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15896) [M::mem_pestat] mean and std.dev: (4100.16, 2851.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 329, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (330.66, 124.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1836, 3892, 6510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15858) [M::mem_pestat] mean and std.dev: (4252.58, 2794.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20532) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1634, 3744, 6454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16094) [M::mem_pestat] mean and std.dev: (4168.83, 2832.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20914) [M::mem_process_seqs] Processed 1333334 reads in 411.366 CPU sec, 26.211 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7408, 129611, 6508, 7035) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1651, 3638, 6369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15805) [M::mem_pestat] mean and std.dev: (4113.20, 2822.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 328, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (330.52, 125.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1795, 3811, 6470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15820) [M::mem_pestat] mean and std.dev: (4216.03, 2809.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20495) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1663, 3624, 6351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15727) [M::mem_pestat] mean and std.dev: (4136.31, 2818.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20415) [M::mem_process_seqs] Processed 1333334 reads in 415.129 CPU sec, 26.507 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7509, 128206, 6834, 7153) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1606, 3587, 6276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15616) [M::mem_pestat] mean and std.dev: (4070.36, 2817.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20286) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 328, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (330.46, 124.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1866, 3930, 6602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16074) [M::mem_pestat] mean and std.dev: (4300.81, 2789.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20810) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1633, 3693, 6302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15640) [M::mem_pestat] mean and std.dev: (4121.45, 2803.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20309) [M::mem_process_seqs] Processed 1333334 reads in 410.019 CPU sec, 26.030 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7381, 127420, 6723, 7168) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1601, 3628, 6403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16007) [M::mem_pestat] mean and std.dev: (4103.75, 2846.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 328, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (329.30, 124.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1868, 3885, 6493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15743) [M::mem_pestat] mean and std.dev: (4278.49, 2782.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20368) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1654, 3675, 6338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15706) [M::mem_pestat] mean and std.dev: (4147.01, 2836.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20390) [M::mem_process_seqs] Processed 1333334 reads in 447.918 CPU sec, 28.697 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7310, 127115, 6482, 7226) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1621, 3701, 6491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16231) [M::mem_pestat] mean and std.dev: (4155.20, 2846.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21101) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 328, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (330.03, 125.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1788, 3798, 6499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15921) [M::mem_pestat] mean and std.dev: (4247.90, 2819.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20632) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1598, 3644, 6288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15668) [M::mem_pestat] mean and std.dev: (4091.90, 2788.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20358) [M::mem_process_seqs] Processed 1333334 reads in 433.945 CPU sec, 27.645 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7230, 127604, 6783, 7197) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1599, 3690, 6363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15891) [M::mem_pestat] mean and std.dev: (4117.37, 2838.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 329, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (330.86, 126.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1829, 3837, 6565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16037) [M::mem_pestat] mean and std.dev: (4270.39, 2808.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20773) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1697, 3700, 6383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15755) [M::mem_pestat] mean and std.dev: (4165.64, 2811.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20441) [M::mem_process_seqs] Processed 1333334 reads in 418.433 CPU sec, 26.604 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7310, 127937, 6558, 7031) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1644, 3732, 6451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16065) [M::mem_pestat] mean and std.dev: (4159.16, 2839.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 330, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (332.56, 126.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1803, 3962, 6661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16377) [M::mem_pestat] mean and std.dev: (4310.66, 2832.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21235) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1601, 3649, 6439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16115) [M::mem_pestat] mean and std.dev: (4131.51, 2835.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20953) [M::mem_process_seqs] Processed 1333334 reads in 419.922 CPU sec, 26.748 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7306, 126767, 6692, 6978) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1666, 3651, 6490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16138) [M::mem_pestat] mean and std.dev: (4159.15, 2855.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20962) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 329, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (331.35, 126.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1803, 3872, 6489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15861) [M::mem_pestat] mean and std.dev: (4256.45, 2795.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20547) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1581, 3637, 6285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15693) [M::mem_pestat] mean and std.dev: (4080.58, 2795.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20397) [M::mem_process_seqs] Processed 1333334 reads in 442.572 CPU sec, 28.362 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7196, 127286, 6564, 7148) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1587, 3584, 6226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15504) [M::mem_pestat] mean and std.dev: (4046.14, 2801.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20143) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 330, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (332.16, 126.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1872, 3847, 6529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15843) [M::mem_pestat] mean and std.dev: (4283.29, 2793.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20500) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1647, 3727, 6296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15594) [M::mem_pestat] mean and std.dev: (4145.30, 2813.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20243) [M::mem_process_seqs] Processed 1333334 reads in 417.983 CPU sec, 26.739 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7279, 127936, 6738, 7205) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1605, 3618, 6275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15615) [M::mem_pestat] mean and std.dev: (4088.34, 2818.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20285) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (332.94, 127.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1867, 3932, 6595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16051) [M::mem_pestat] mean and std.dev: (4294.38, 2809.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20779) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1667, 3671, 6490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16136) [M::mem_pestat] mean and std.dev: (4164.39, 2833.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20959) [M::mem_process_seqs] Processed 1333334 reads in 406.079 CPU sec, 25.807 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7362, 127587, 6511, 6997) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1566, 3662, 6306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15786) [M::mem_pestat] mean and std.dev: (4086.05, 2843.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 329, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (331.99, 127.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1836, 3869, 6589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16095) [M::mem_pestat] mean and std.dev: (4278.28, 2807.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20848) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1616, 3629, 6349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15815) [M::mem_pestat] mean and std.dev: (4113.83, 2829.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20548) [M::mem_process_seqs] Processed 1333334 reads in 415.342 CPU sec, 26.397 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7386, 126862, 6644, 7144) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1585, 3662, 6372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15946) [M::mem_pestat] mean and std.dev: (4095.95, 2836.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 331, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (333.32, 127.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1906, 3959, 6721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16351) [M::mem_pestat] mean and std.dev: (4346.93, 2814.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21166) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1638, 3793, 6498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16218) [M::mem_pestat] mean and std.dev: (4195.18, 2845.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21078) [M::mem_process_seqs] Processed 1333334 reads in 403.656 CPU sec, 25.742 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7344, 127213, 6657, 7035) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1588, 3641, 6284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15676) [M::mem_pestat] mean and std.dev: (4081.22, 2812.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20372) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 331, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (333.15, 127.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1819, 3792, 6473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15781) [M::mem_pestat] mean and std.dev: (4239.83, 2783.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20435) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1631, 3561, 6288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15602) [M::mem_pestat] mean and std.dev: (4086.04, 2807.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20259) [M::mem_process_seqs] Processed 1333334 reads in 410.001 CPU sec, 26.150 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7276, 127359, 6662, 7035) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1592, 3732, 6394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15998) [M::mem_pestat] mean and std.dev: (4125.06, 2825.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 330, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (332.82, 127.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1932, 4026, 6657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16107) [M::mem_pestat] mean and std.dev: (4371.25, 2811.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20832) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1663, 3665, 6398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15868) [M::mem_pestat] mean and std.dev: (4145.59, 2812.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20603) [M::mem_process_seqs] Processed 1333334 reads in 416.323 CPU sec, 26.454 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7318, 125619, 6613, 7221) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1552, 3611, 6328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15880) [M::mem_pestat] mean and std.dev: (4099.22, 2857.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 330, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (331.73, 126.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1841, 3895, 6669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16325) [M::mem_pestat] mean and std.dev: (4323.42, 2835.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21153) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1692, 3649, 6405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15831) [M::mem_pestat] mean and std.dev: (4152.54, 2795.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20544) [M::mem_process_seqs] Processed 1333334 reads in 453.593 CPU sec, 29.007 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7351, 126939, 6733, 7076) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1586, 3576, 6386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15986) [M::mem_pestat] mean and std.dev: (4084.51, 2850.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 331, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (333.56, 128.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1864, 3930, 6574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15994) [M::mem_pestat] mean and std.dev: (4284.09, 2801.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20704) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1748, 3854, 6548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16148) [M::mem_pestat] mean and std.dev: (4231.74, 2822.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20948) [M::mem_process_seqs] Processed 1333334 reads in 411.251 CPU sec, 26.200 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7372, 127130, 6714, 7242) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1611, 3677, 6357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15849) [M::mem_pestat] mean and std.dev: (4121.70, 2835.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 330, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (332.95, 127.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1962, 3947, 6542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15702) [M::mem_pestat] mean and std.dev: (4321.98, 2773.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20282) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1569, 3576, 6281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15705) [M::mem_pestat] mean and std.dev: (4059.28, 2809.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20417) [M::mem_process_seqs] Processed 1333334 reads in 412.701 CPU sec, 26.322 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7113, 126313, 6737, 7200) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1549, 3635, 6265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15697) [M::mem_pestat] mean and std.dev: (4059.95, 2806.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 331, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (333.18, 128.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1859, 3939, 6572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15998) [M::mem_pestat] mean and std.dev: (4301.56, 2805.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20711) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1644, 3675, 6334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15714) [M::mem_pestat] mean and std.dev: (4141.07, 2811.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20404) [M::mem_process_seqs] Processed 1333334 reads in 407.244 CPU sec, 25.943 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7323, 126487, 6766, 6839) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1568, 3647, 6378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15998) [M::mem_pestat] mean and std.dev: (4102.02, 2841.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 330, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (332.71, 127.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1819, 3901, 6535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15967) [M::mem_pestat] mean and std.dev: (4267.69, 2802.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20683) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1684, 3756, 6415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15877) [M::mem_pestat] mean and std.dev: (4186.54, 2824.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20608) [M::mem_process_seqs] Processed 1333334 reads in 429.961 CPU sec, 27.352 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7320, 130736, 6603, 6989) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1494, 3544, 6247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15753) [M::mem_pestat] mean and std.dev: (4000.00, 2834.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (239, 322, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (323.81, 123.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1843, 3979, 6719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16471) [M::mem_pestat] mean and std.dev: (4341.07, 2838.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21347) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1596, 3629, 6407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16029) [M::mem_pestat] mean and std.dev: (4117.80, 2838.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20840) [M::mem_process_seqs] Processed 1333334 reads in 457.655 CPU sec, 29.310 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7384, 129937, 6766, 7008) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1656, 3700, 6418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15942) [M::mem_pestat] mean and std.dev: (4134.12, 2827.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (238, 321, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (323.20, 122.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1834, 3908, 6576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16060) [M::mem_pestat] mean and std.dev: (4303.79, 2816.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20802) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1666, 3566, 6313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15607) [M::mem_pestat] mean and std.dev: (4112.99, 2813.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20254) [M::mem_process_seqs] Processed 1333334 reads in 454.209 CPU sec, 29.078 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7260, 131491, 6811, 6982) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1565, 3701, 6318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15824) [M::mem_pestat] mean and std.dev: (4075.52, 2817.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (239, 321, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (323.82, 121.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1755, 3835, 6602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16296) [M::mem_pestat] mean and std.dev: (4251.86, 2847.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21143) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1641, 3677, 6361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15801) [M::mem_pestat] mean and std.dev: (4141.75, 2815.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20521) [M::mem_process_seqs] Processed 1333334 reads in 436.502 CPU sec, 27.840 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7381, 131263, 6682, 7281) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1604, 3647, 6424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16064) [M::mem_pestat] mean and std.dev: (4129.15, 2847.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (238, 320, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (323.03, 122.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1832, 3865, 6453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15695) [M::mem_pestat] mean and std.dev: (4254.15, 2798.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20316) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1665, 3699, 6407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15891) [M::mem_pestat] mean and std.dev: (4164.67, 2828.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20633) [M::mem_process_seqs] Processed 1333334 reads in 460.443 CPU sec, 29.305 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7377, 131165, 6589, 7177) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1566, 3645, 6504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16380) [M::mem_pestat] mean and std.dev: (4128.58, 2864.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (239, 321, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (324.14, 122.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1861, 3889, 6528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15862) [M::mem_pestat] mean and std.dev: (4296.31, 2807.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20529) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1640, 3614, 6332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15716) [M::mem_pestat] mean and std.dev: (4112.73, 2818.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20408) [M::mem_process_seqs] Processed 1333334 reads in 431.366 CPU sec, 27.478 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7350, 130869, 6643, 7053) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1578, 3608, 6384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15996) [M::mem_pestat] mean and std.dev: (4092.02, 2830.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (239, 321, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (324.12, 122.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1914, 3949, 6556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15840) [M::mem_pestat] mean and std.dev: (4314.63, 2773.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20482) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1637, 3692, 6335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15731) [M::mem_pestat] mean and std.dev: (4133.62, 2796.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20429) [M::mem_process_seqs] Processed 1333334 reads in 436.435 CPU sec, 27.739 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7363, 128458, 6538, 6883) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1610, 3655, 6383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15929) [M::mem_pestat] mean and std.dev: (4117.42, 2834.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (239, 322, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (324.57, 122.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1865, 4014, 6582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16016) [M::mem_pestat] mean and std.dev: (4318.14, 2793.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20733) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1615, 3654, 6326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15748) [M::mem_pestat] mean and std.dev: (4086.91, 2802.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20459) [M::mem_process_seqs] Processed 1333334 reads in 463.993 CPU sec, 29.985 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7515, 130911, 6627, 7128) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1606, 3649, 6312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15724) [M::mem_pestat] mean and std.dev: (4080.85, 2809.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (241, 324, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (325.91, 122.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1852, 3964, 6516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15844) [M::mem_pestat] mean and std.dev: (4281.29, 2778.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20508) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1613, 3668, 6373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15893) [M::mem_pestat] mean and std.dev: (4137.13, 2830.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20653) [M::mem_process_seqs] Processed 1333334 reads in 438.456 CPU sec, 27.942 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7242, 130486, 6657, 7215) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1596, 3667, 6355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15873) [M::mem_pestat] mean and std.dev: (4103.33, 2814.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 325, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (327.10, 123.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1823, 3841, 6424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15626) [M::mem_pestat] mean and std.dev: (4227.78, 2784.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20227) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1612, 3608, 6332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15772) [M::mem_pestat] mean and std.dev: (4123.90, 2834.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20492) [M::mem_process_seqs] Processed 1333334 reads in 423.261 CPU sec, 26.932 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7295, 127645, 6610, 7183) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1608, 3696, 6355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15849) [M::mem_pestat] mean and std.dev: (4116.44, 2835.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (240, 322, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (324.34, 122.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1826, 3889, 6528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15932) [M::mem_pestat] mean and std.dev: (4264.68, 2793.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20634) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1578, 3615, 6365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15939) [M::mem_pestat] mean and std.dev: (4096.42, 2830.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20726) [M::mem_process_seqs] Processed 1333334 reads in 475.716 CPU sec, 30.386 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7296, 130995, 6621, 7279) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1603, 3588, 6368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15898) [M::mem_pestat] mean and std.dev: (4113.21, 2849.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 324, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (327.65, 123.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1878, 3874, 6583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15993) [M::mem_pestat] mean and std.dev: (4293.23, 2813.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20698) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1637, 3590, 6331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15719) [M::mem_pestat] mean and std.dev: (4100.66, 2810.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20413) [M::mem_process_seqs] Processed 1333334 reads in 415.775 CPU sec, 26.725 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7307, 130391, 6627, 7122) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1629, 3626, 6416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15990) [M::mem_pestat] mean and std.dev: (4110.30, 2830.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (241, 325, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (327.48, 123.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1825, 3992, 6589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16117) [M::mem_pestat] mean and std.dev: (4305.67, 2798.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20881) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1617, 3619, 6288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15630) [M::mem_pestat] mean and std.dev: (4100.52, 2814.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20301) [M::mem_process_seqs] Processed 1333334 reads in 412.178 CPU sec, 26.231 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7315, 127614, 6708, 7071) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1587, 3702, 6359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15903) [M::mem_pestat] mean and std.dev: (4122.96, 2831.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (241, 324, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (326.47, 122.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1821, 3848, 6540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15978) [M::mem_pestat] mean and std.dev: (4263.00, 2797.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20697) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1649, 3594, 6271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15515) [M::mem_pestat] mean and std.dev: (4092.89, 2775.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20137) [M::mem_process_seqs] Processed 1333334 reads in 457.589 CPU sec, 29.046 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7354, 130075, 6606, 7105) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1594, 3700, 6321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15775) [M::mem_pestat] mean and std.dev: (4086.98, 2809.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 325, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (328.30, 124.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1830, 3860, 6474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15762) [M::mem_pestat] mean and std.dev: (4263.43, 2795.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20406) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1694, 3841, 6533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16211) [M::mem_pestat] mean and std.dev: (4240.44, 2846.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21050) [M::mem_process_seqs] Processed 1333334 reads in 410.682 CPU sec, 26.177 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7261, 128518, 6494, 7168) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1592, 3732, 6478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16250) [M::mem_pestat] mean and std.dev: (4159.43, 2858.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21136) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (241, 324, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (326.70, 123.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1819, 3863, 6487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15823) [M::mem_pestat] mean and std.dev: (4253.37, 2788.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20491) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1638, 3702, 6392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15900) [M::mem_pestat] mean and std.dev: (4155.03, 2819.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20654) [M::mem_process_seqs] Processed 1333334 reads in 459.079 CPU sec, 29.261 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7253, 128588, 6675, 6994) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1564, 3602, 6331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15865) [M::mem_pestat] mean and std.dev: (4066.54, 2835.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (241, 324, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (327.21, 123.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1885, 3895, 6639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16147) [M::mem_pestat] mean and std.dev: (4299.98, 2798.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20901) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1569, 3585, 6334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15864) [M::mem_pestat] mean and std.dev: (4095.02, 2837.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20629) [M::mem_process_seqs] Processed 1333334 reads in 448.463 CPU sec, 28.559 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7291, 128724, 6718, 6970) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1551, 3635, 6418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16152) [M::mem_pestat] mean and std.dev: (4116.40, 2856.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21019) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (241, 325, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (327.37, 124.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1825, 3818, 6456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15718) [M::mem_pestat] mean and std.dev: (4235.75, 2798.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20349) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1643, 3683, 6452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16070) [M::mem_pestat] mean and std.dev: (4150.02, 2818.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20879) [M::mem_process_seqs] Processed 1333334 reads in 448.760 CPU sec, 28.550 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7299, 127543, 6687, 7026) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1617, 3705, 6424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16038) [M::mem_pestat] mean and std.dev: (4125.19, 2818.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (240, 325, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (327.07, 124.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1855, 3908, 6544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15922) [M::mem_pestat] mean and std.dev: (4272.39, 2795.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20611) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1630, 3725, 6343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15769) [M::mem_pestat] mean and std.dev: (4119.14, 2799.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20482) [M::mem_process_seqs] Processed 1333334 reads in 453.827 CPU sec, 28.926 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7335, 129156, 6799, 7094) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1627, 3706, 6392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15922) [M::mem_pestat] mean and std.dev: (4129.34, 2826.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 326, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (328.73, 124.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1826, 3952, 6631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16241) [M::mem_pestat] mean and std.dev: (4318.69, 2799.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21046) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1597, 3615, 6311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15739) [M::mem_pestat] mean and std.dev: (4086.48, 2816.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20453) [M::mem_process_seqs] Processed 1333334 reads in 433.257 CPU sec, 27.584 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7466, 130193, 6715, 7110) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1643, 3647, 6334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15716) [M::mem_pestat] mean and std.dev: (4092.60, 2820.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 327, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (329.28, 124.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1815, 3868, 6476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15798) [M::mem_pestat] mean and std.dev: (4254.23, 2790.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20459) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1675, 3678, 6372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15766) [M::mem_pestat] mean and std.dev: (4146.24, 2820.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20463) [M::mem_process_seqs] Processed 1333334 reads in 415.334 CPU sec, 26.447 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7361, 129312, 6667, 7180) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1584, 3659, 6358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15906) [M::mem_pestat] mean and std.dev: (4121.93, 2848.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (241, 326, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (328.42, 124.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1920, 3947, 6559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15837) [M::mem_pestat] mean and std.dev: (4329.25, 2792.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20476) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1650, 3588, 6298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15594) [M::mem_pestat] mean and std.dev: (4089.09, 2788.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20242) [M::mem_process_seqs] Processed 1333334 reads in 437.252 CPU sec, 27.748 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7325, 129556, 6627, 7204) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1567, 3678, 6380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16006) [M::mem_pestat] mean and std.dev: (4088.64, 2815.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 327, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (329.42, 124.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1837, 3911, 6497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15817) [M::mem_pestat] mean and std.dev: (4272.04, 2795.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1607, 3669, 6293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15665) [M::mem_pestat] mean and std.dev: (4097.65, 2804.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20351) [M::mem_process_seqs] Processed 1333334 reads in 410.509 CPU sec, 26.164 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7305, 129358, 6754, 7175) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1586, 3654, 6416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16076) [M::mem_pestat] mean and std.dev: (4126.93, 2860.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 327, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (329.65, 125.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1912, 4047, 6623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16045) [M::mem_pestat] mean and std.dev: (4343.73, 2805.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20756) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1598, 3620, 6370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15914) [M::mem_pestat] mean and std.dev: (4122.48, 2837.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20686) [M::mem_process_seqs] Processed 1333334 reads in 408.960 CPU sec, 26.003 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7400, 128970, 6755, 7240) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1569, 3685, 6335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15867) [M::mem_pestat] mean and std.dev: (4103.94, 2830.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 328, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (329.94, 125.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1834, 4033, 6556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16000) [M::mem_pestat] mean and std.dev: (4314.34, 2795.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20722) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1657, 3659, 6353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15745) [M::mem_pestat] mean and std.dev: (4119.20, 2819.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20441) [M::mem_process_seqs] Processed 1333334 reads in 411.789 CPU sec, 26.237 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7626, 128554, 6834, 7207) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1581, 3572, 6174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15360) [M::mem_pestat] mean and std.dev: (4023.50, 2791.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 326, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (328.12, 123.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1859, 3939, 6541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15905) [M::mem_pestat] mean and std.dev: (4289.02, 2793.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20587) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1714, 3789, 6302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15478) [M::mem_pestat] mean and std.dev: (4171.31, 2780.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20066) [M::mem_process_seqs] Processed 1333334 reads in 457.289 CPU sec, 29.015 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7267, 129038, 6500, 7264) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1574, 3589, 6369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15959) [M::mem_pestat] mean and std.dev: (4089.75, 2838.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 329, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (331.53, 126.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1883, 3884, 6559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15911) [M::mem_pestat] mean and std.dev: (4293.71, 2795.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20587) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1637, 3620, 6268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15530) [M::mem_pestat] mean and std.dev: (4097.11, 2790.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20161) [M::mem_process_seqs] Processed 1333334 reads in 413.939 CPU sec, 26.461 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7299, 128376, 6750, 7277) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1638, 3603, 6384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15876) [M::mem_pestat] mean and std.dev: (4117.17, 2827.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 329, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (331.11, 125.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1903, 3889, 6480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15634) [M::mem_pestat] mean and std.dev: (4266.29, 2777.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20211) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1637, 3697, 6368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15830) [M::mem_pestat] mean and std.dev: (4142.31, 2832.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20561) [M::mem_process_seqs] Processed 1333334 reads in 418.633 CPU sec, 26.696 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7447, 129083, 6773, 7167) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1631, 3623, 6373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15857) [M::mem_pestat] mean and std.dev: (4115.51, 2829.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 328, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (330.59, 125.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1858, 3890, 6489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15751) [M::mem_pestat] mean and std.dev: (4263.08, 2793.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1682, 3684, 6328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15620) [M::mem_pestat] mean and std.dev: (4150.29, 2816.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20266) [M::mem_process_seqs] Processed 1333334 reads in 416.333 CPU sec, 26.586 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7372, 129274, 6645, 7243) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1591, 3600, 6352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15874) [M::mem_pestat] mean and std.dev: (4094.03, 2844.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 328, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (330.68, 126.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1880, 3914, 6622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16106) [M::mem_pestat] mean and std.dev: (4300.78, 2812.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20848) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1597, 3702, 6424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16078) [M::mem_pestat] mean and std.dev: (4130.48, 2825.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20905) [M::mem_process_seqs] Processed 1333334 reads in 411.270 CPU sec, 26.208 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7562, 130497, 6678, 7073) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1597, 3678, 6361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15889) [M::mem_pestat] mean and std.dev: (4111.51, 2837.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (240, 325, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (326.38, 124.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1881, 3930, 6627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16119) [M::mem_pestat] mean and std.dev: (4305.11, 2811.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20865) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1681, 3751, 6423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15907) [M::mem_pestat] mean and std.dev: (4153.75, 2800.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20649) [M::mem_process_seqs] Processed 1333334 reads in 458.193 CPU sec, 29.103 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7402, 127582, 6728, 7274) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1612, 3588, 6290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15646) [M::mem_pestat] mean and std.dev: (4082.76, 2839.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (241, 326, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (327.68, 124.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1826, 3878, 6577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16079) [M::mem_pestat] mean and std.dev: (4281.84, 2818.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20830) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1629, 3644, 6422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16008) [M::mem_pestat] mean and std.dev: (4146.70, 2841.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20801) [M::mem_process_seqs] Processed 1333334 reads in 467.601 CPU sec, 29.817 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7462, 130915, 6741, 7243) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1598, 3612, 6357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15875) [M::mem_pestat] mean and std.dev: (4103.09, 2832.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 326, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (328.32, 124.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1841, 3901, 6574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16040) [M::mem_pestat] mean and std.dev: (4288.88, 2827.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20773) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1692, 3894, 6496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16104) [M::mem_pestat] mean and std.dev: (4234.05, 2821.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20908) [M::mem_process_seqs] Processed 1333334 reads in 415.888 CPU sec, 26.550 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7441, 129302, 6693, 7243) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1599, 3616, 6485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16257) [M::mem_pestat] mean and std.dev: (4131.96, 2858.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21143) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 326, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (328.87, 124.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1867, 3962, 6554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15928) [M::mem_pestat] mean and std.dev: (4292.94, 2779.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20615) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1615, 3625, 6325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15745) [M::mem_pestat] mean and std.dev: (4083.18, 2790.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20455) [M::mem_process_seqs] Processed 1333334 reads in 461.128 CPU sec, 29.344 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7415, 130532, 6676, 7021) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1596, 3571, 6295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15693) [M::mem_pestat] mean and std.dev: (4063.58, 2806.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 327, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (329.63, 124.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1856, 4042, 6616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16136) [M::mem_pestat] mean and std.dev: (4319.56, 2804.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20896) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1645, 3683, 6269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15517) [M::mem_pestat] mean and std.dev: (4103.17, 2773.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20141) [M::mem_process_seqs] Processed 1333334 reads in 426.575 CPU sec, 27.381 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7412, 129368, 6756, 7250) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1651, 3741, 6429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15985) [M::mem_pestat] mean and std.dev: (4165.57, 2831.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 328, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (330.40, 124.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1739, 3820, 6548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16166) [M::mem_pestat] mean and std.dev: (4223.53, 2837.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20975) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1666, 3588, 6391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15841) [M::mem_pestat] mean and std.dev: (4142.61, 2830.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20566) [M::mem_process_seqs] Processed 1333334 reads in 439.613 CPU sec, 28.047 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7492, 124699, 6578, 7355) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1567, 3554, 6355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15931) [M::mem_pestat] mean and std.dev: (4074.22, 2845.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 329, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (330.23, 125.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1805, 3817, 6373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15509) [M::mem_pestat] mean and std.dev: (4198.19, 2765.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20077) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1656, 3722, 6379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15825) [M::mem_pestat] mean and std.dev: (4149.47, 2802.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20548) [M::mem_process_seqs] Processed 1333334 reads in 479.065 CPU sec, 30.808 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7515, 128975, 6819, 7304) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1571, 3614, 6369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15965) [M::mem_pestat] mean and std.dev: (4080.08, 2841.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 329, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (331.05, 125.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1914, 3988, 6699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16269) [M::mem_pestat] mean and std.dev: (4352.24, 2804.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21054) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1657, 3671, 6353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15745) [M::mem_pestat] mean and std.dev: (4138.65, 2816.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20441) [M::mem_process_seqs] Processed 1333334 reads in 436.239 CPU sec, 27.770 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7428, 129024, 6578, 7275) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1516, 3512, 6267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15769) [M::mem_pestat] mean and std.dev: (4029.54, 2835.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 328, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (330.80, 125.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1878, 3900, 6589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16011) [M::mem_pestat] mean and std.dev: (4297.66, 2794.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20722) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1671, 3733, 6380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15798) [M::mem_pestat] mean and std.dev: (4162.47, 2787.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20507) [M::mem_process_seqs] Processed 1333334 reads in 420.342 CPU sec, 26.847 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7348, 126179, 6840, 7330) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1565, 3645, 6357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15941) [M::mem_pestat] mean and std.dev: (4087.66, 2827.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 328, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (330.09, 125.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1828, 3941, 6618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16198) [M::mem_pestat] mean and std.dev: (4307.74, 2811.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20988) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1682, 3672, 6365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15731) [M::mem_pestat] mean and std.dev: (4154.31, 2819.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20414) [M::mem_process_seqs] Processed 1333334 reads in 474.869 CPU sec, 30.275 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7505, 129154, 6876, 7440) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1559, 3631, 6350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15932) [M::mem_pestat] mean and std.dev: (4101.53, 2834.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20723) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (333.05, 126.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1848, 3917, 6476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15732) [M::mem_pestat] mean and std.dev: (4254.24, 2770.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20360) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1650, 3679, 6411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15933) [M::mem_pestat] mean and std.dev: (4130.62, 2816.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20694) [M::mem_process_seqs] Processed 1333334 reads in 410.686 CPU sec, 26.209 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7318, 129241, 6775, 7452) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1596, 3729, 6411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16041) [M::mem_pestat] mean and std.dev: (4135.37, 2824.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 330, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (332.50, 126.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1841, 3930, 6650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16268) [M::mem_pestat] mean and std.dev: (4316.65, 2826.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21077) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1664, 3758, 6352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15728) [M::mem_pestat] mean and std.dev: (4148.52, 2797.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20416) [M::mem_process_seqs] Processed 1333334 reads in 404.648 CPU sec, 25.768 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7406, 128513, 6655, 7231) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1609, 3581, 6365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15877) [M::mem_pestat] mean and std.dev: (4091.65, 2835.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 330, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (332.17, 126.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1868, 3941, 6604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16076) [M::mem_pestat] mean and std.dev: (4306.20, 2799.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20812) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1680, 3662, 6354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15702) [M::mem_pestat] mean and std.dev: (4116.02, 2783.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20376) [M::mem_process_seqs] Processed 1333334 reads in 432.197 CPU sec, 27.442 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7501, 129081, 6808, 7168) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1709, 3834, 6515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16127) [M::mem_pestat] mean and std.dev: (4233.26, 2853.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (333.45, 126.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1792, 3901, 6568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16120) [M::mem_pestat] mean and std.dev: (4282.90, 2822.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20896) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1729, 3732, 6515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16087) [M::mem_pestat] mean and std.dev: (4199.22, 2821.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20873) [M::mem_process_seqs] Processed 1333334 reads in 409.984 CPU sec, 26.134 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7426, 127799, 6546, 7113) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1571, 3655, 6442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16184) [M::mem_pestat] mean and std.dev: (4118.28, 2848.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21055) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 330, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (332.25, 126.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1911, 3872, 6594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15960) [M::mem_pestat] mean and std.dev: (4297.50, 2801.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20643) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1719, 3833, 6404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15774) [M::mem_pestat] mean and std.dev: (4194.08, 2812.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20459) [M::mem_process_seqs] Processed 1333334 reads in 451.628 CPU sec, 28.670 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7425, 127681, 6661, 7193) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1587, 3623, 6327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15807) [M::mem_pestat] mean and std.dev: (4089.22, 2824.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 333, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (334.95, 127.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1868, 3912, 6557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15935) [M::mem_pestat] mean and std.dev: (4287.94, 2798.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20624) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1593, 3632, 6363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15903) [M::mem_pestat] mean and std.dev: (4111.61, 2829.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20673) [M::mem_process_seqs] Processed 1333334 reads in 422.276 CPU sec, 26.852 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7541, 127267, 6592, 7311) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1598, 3683, 6468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16208) [M::mem_pestat] mean and std.dev: (4142.01, 2832.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21078) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 332, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (334.66, 127.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1861, 3894, 6639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16195) [M::mem_pestat] mean and std.dev: (4316.73, 2818.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20973) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1646, 3678, 6276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15536) [M::mem_pestat] mean and std.dev: (4136.42, 2827.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20166) [M::mem_process_seqs] Processed 1333334 reads in 434.322 CPU sec, 27.585 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7365, 124772, 6759, 7157) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1516, 3561, 6287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15829) [M::mem_pestat] mean and std.dev: (4043.24, 2831.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (333.44, 127.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1842, 3995, 6662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16302) [M::mem_pestat] mean and std.dev: (4331.33, 2834.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21122) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1627, 3695, 6389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15913) [M::mem_pestat] mean and std.dev: (4142.52, 2798.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20675) [M::mem_process_seqs] Processed 1333334 reads in 459.316 CPU sec, 29.179 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7526, 128694, 6582, 7139) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1624, 3752, 6526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16330) [M::mem_pestat] mean and std.dev: (4174.85, 2850.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21232) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 332, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (334.12, 127.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1867, 3922, 6477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15697) [M::mem_pestat] mean and std.dev: (4251.66, 2766.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1587, 3685, 6302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15732) [M::mem_pestat] mean and std.dev: (4099.38, 2816.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20447) [M::mem_process_seqs] Processed 1333334 reads in 416.746 CPU sec, 26.481 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7439, 128872, 6683, 7210) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1589, 3705, 6322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15788) [M::mem_pestat] mean and std.dev: (4098.79, 2814.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 332, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (335.04, 127.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1782, 3792, 6456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15804) [M::mem_pestat] mean and std.dev: (4214.44, 2810.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20478) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1682, 3755, 6383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15785) [M::mem_pestat] mean and std.dev: (4163.74, 2806.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20486) [M::mem_process_seqs] Processed 1333334 reads in 403.775 CPU sec, 25.660 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7561, 126981, 6780, 7191) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1618, 3685, 6406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15982) [M::mem_pestat] mean and std.dev: (4131.10, 2841.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 332, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (333.95, 127.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1782, 3791, 6552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16092) [M::mem_pestat] mean and std.dev: (4228.69, 2801.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1614, 3731, 6463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16161) [M::mem_pestat] mean and std.dev: (4162.58, 2832.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21010) [M::mem_process_seqs] Processed 1333334 reads in 434.302 CPU sec, 27.759 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7493, 128197, 6853, 7245) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1603, 3684, 6418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16048) [M::mem_pestat] mean and std.dev: (4120.50, 2823.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 334, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (335.76, 128.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1874, 3979, 6553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15911) [M::mem_pestat] mean and std.dev: (4295.91, 2786.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20590) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1604, 3588, 6377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15923) [M::mem_pestat] mean and std.dev: (4115.48, 2833.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20696) [M::mem_process_seqs] Processed 1333334 reads in 398.500 CPU sec, 25.310 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7596, 128723, 6637, 7193) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1583, 3724, 6453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16193) [M::mem_pestat] mean and std.dev: (4133.38, 2842.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21063) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (334.41, 128.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1864, 3882, 6587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16033) [M::mem_pestat] mean and std.dev: (4278.50, 2798.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20756) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1710, 3767, 6468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15984) [M::mem_pestat] mean and std.dev: (4198.43, 2820.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20742) [M::mem_process_seqs] Processed 1333334 reads in 402.499 CPU sec, 25.614 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7417, 128256, 6622, 7340) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1626, 3692, 6425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16023) [M::mem_pestat] mean and std.dev: (4133.34, 2849.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 333, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (335.08, 128.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1868, 3955, 6685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16319) [M::mem_pestat] mean and std.dev: (4329.92, 2819.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21136) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1649, 3687, 6423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15971) [M::mem_pestat] mean and std.dev: (4170.94, 2832.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20745) [M::mem_process_seqs] Processed 1333334 reads in 414.977 CPU sec, 26.666 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7363, 126005, 6666, 7180) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1588, 3724, 6383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15973) [M::mem_pestat] mean and std.dev: (4134.28, 2843.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 332, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (334.17, 126.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1836, 3892, 6573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16047) [M::mem_pestat] mean and std.dev: (4297.96, 2817.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20784) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1651, 3587, 6303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15607) [M::mem_pestat] mean and std.dev: (4090.10, 2797.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20259) [M::mem_process_seqs] Processed 1333334 reads in 458.550 CPU sec, 29.255 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7653, 128511, 6740, 7192) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1585, 3686, 6371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15943) [M::mem_pestat] mean and std.dev: (4111.43, 2830.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 334, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (335.96, 128.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1759, 3845, 6568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16186) [M::mem_pestat] mean and std.dev: (4249.46, 2830.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20995) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1702, 3770, 6422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15862) [M::mem_pestat] mean and std.dev: (4180.69, 2812.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20582) [M::mem_process_seqs] Processed 1333334 reads in 400.162 CPU sec, 25.502 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7388, 127448, 6665, 7327) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1612, 3684, 6415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16021) [M::mem_pestat] mean and std.dev: (4142.66, 2836.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 333, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (334.77, 127.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1764, 3918, 6493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15951) [M::mem_pestat] mean and std.dev: (4230.86, 2796.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20680) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1674, 3760, 6457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16023) [M::mem_pestat] mean and std.dev: (4191.09, 2820.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20806) [M::mem_process_seqs] Processed 1333334 reads in 421.814 CPU sec, 26.990 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7371, 128149, 6777, 7205) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1715, 3732, 6423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15839) [M::mem_pestat] mean and std.dev: (4168.73, 2825.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 333, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (335.76, 128.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1814, 3834, 6481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15815) [M::mem_pestat] mean and std.dev: (4249.96, 2794.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20482) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1710, 3712, 6445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15915) [M::mem_pestat] mean and std.dev: (4172.65, 2810.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20650) [M::mem_process_seqs] Processed 1333334 reads in 398.953 CPU sec, 25.379 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7520, 127341, 6559, 7129) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1643, 3666, 6407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15935) [M::mem_pestat] mean and std.dev: (4137.03, 2827.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 332, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (334.56, 128.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1764, 3745, 6460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15852) [M::mem_pestat] mean and std.dev: (4204.45, 2818.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20548) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1660, 3704, 6420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15940) [M::mem_pestat] mean and std.dev: (4167.29, 2813.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20700) [M::mem_process_seqs] Processed 1333334 reads in 403.371 CPU sec, 25.660 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7420, 131681, 6802, 7411) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1586, 3692, 6342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15854) [M::mem_pestat] mean and std.dev: (4100.01, 2827.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (240, 324, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (326.80, 124.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1828, 3859, 6592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16120) [M::mem_pestat] mean and std.dev: (4285.82, 2810.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20884) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1638, 3730, 6427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16005) [M::mem_pestat] mean and std.dev: (4160.14, 2833.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20794) [M::mem_process_seqs] Processed 1333334 reads in 448.761 CPU sec, 28.595 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7521, 130911, 6728, 7255) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1570, 3634, 6340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15880) [M::mem_pestat] mean and std.dev: (4087.13, 2827.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (239, 323, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (325.24, 123.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1868, 3971, 6570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15974) [M::mem_pestat] mean and std.dev: (4298.04, 2796.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20676) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1603, 3615, 6243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15523) [M::mem_pestat] mean and std.dev: (4074.97, 2785.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20163) [M::mem_process_seqs] Processed 1333334 reads in 460.000 CPU sec, 29.302 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7466, 133001, 6778, 7302) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1588, 3657, 6376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15952) [M::mem_pestat] mean and std.dev: (4102.73, 2836.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (241, 324, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (326.78, 123.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1809, 3903, 6659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16359) [M::mem_pestat] mean and std.dev: (4306.26, 2837.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21209) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1596, 3629, 6276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15636) [M::mem_pestat] mean and std.dev: (4082.14, 2794.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20316) [M::mem_process_seqs] Processed 1333334 reads in 431.854 CPU sec, 27.406 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7376, 130912, 6663, 7096) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1593, 3596, 6395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15999) [M::mem_pestat] mean and std.dev: (4085.86, 2838.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (240, 323, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (326.53, 123.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1872, 3945, 6643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16185) [M::mem_pestat] mean and std.dev: (4320.23, 2816.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20956) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1711, 3760, 6368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15682) [M::mem_pestat] mean and std.dev: (4167.75, 2798.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20339) [M::mem_process_seqs] Processed 1333334 reads in 530.164 CPU sec, 34.066 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7456, 131019, 6670, 7246) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1660, 3754, 6460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16060) [M::mem_pestat] mean and std.dev: (4166.06, 2824.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (241, 325, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (327.37, 123.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1851, 3905, 6479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15735) [M::mem_pestat] mean and std.dev: (4277.63, 2794.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20363) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1662, 3647, 6370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15786) [M::mem_pestat] mean and std.dev: (4133.42, 2805.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20494) [M::mem_process_seqs] Processed 1333334 reads in 488.125 CPU sec, 31.020 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7474, 132693, 6655, 7196) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1621, 3693, 6368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15862) [M::mem_pestat] mean and std.dev: (4117.84, 2827.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (241, 324, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (326.91, 123.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1828, 3947, 6623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16213) [M::mem_pestat] mean and std.dev: (4321.22, 2818.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21008) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1653, 3712, 6379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15831) [M::mem_pestat] mean and std.dev: (4157.78, 2823.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20557) [M::mem_process_seqs] Processed 1333334 reads in 518.300 CPU sec, 32.865 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7372, 121103, 6563, 7108) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1536, 3610, 6316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15876) [M::mem_pestat] mean and std.dev: (4052.95, 2830.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 326, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (327.67, 123.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1867, 3903, 6629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16153) [M::mem_pestat] mean and std.dev: (4309.40, 2822.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20915) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1644, 3721, 6386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15870) [M::mem_pestat] mean and std.dev: (4147.08, 2808.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20612) [M::mem_process_seqs] Processed 1333334 reads in 582.750 CPU sec, 38.768 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7538, 129168, 6931, 7190) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1652, 3725, 6383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15845) [M::mem_pestat] mean and std.dev: (4154.99, 2828.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 327, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (328.99, 124.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1860, 3859, 6557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15951) [M::mem_pestat] mean and std.dev: (4268.80, 2788.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20648) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1640, 3707, 6423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15989) [M::mem_pestat] mean and std.dev: (4161.54, 2833.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20772) [M::mem_process_seqs] Processed 1333334 reads in 472.534 CPU sec, 30.041 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7339, 131412, 6593, 7217) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1669, 3706, 6339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15679) [M::mem_pestat] mean and std.dev: (4123.71, 2804.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 326, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (328.27, 124.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1797, 3943, 6552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16062) [M::mem_pestat] mean and std.dev: (4276.15, 2815.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20817) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1625, 3652, 6316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15698) [M::mem_pestat] mean and std.dev: (4105.88, 2817.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20389) [M::mem_process_seqs] Processed 1333334 reads in 555.604 CPU sec, 35.387 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7228, 117721, 6239, 6764) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1531, 3615, 6479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16375) [M::mem_pestat] mean and std.dev: (4097.72, 2881.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (241, 324, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (326.12, 123.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1888, 3937, 6604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16036) [M::mem_pestat] mean and std.dev: (4302.16, 2803.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20752) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1699, 3732, 6427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15883) [M::mem_pestat] mean and std.dev: (4182.20, 2820.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20611) [M::mem_process_seqs] Processed 1333334 reads in 628.947 CPU sec, 41.894 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7390, 132544, 6506, 7281) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1621, 3720, 6349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15805) [M::mem_pestat] mean and std.dev: (4132.62, 2837.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 326, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (329.12, 124.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1822, 3862, 6582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16102) [M::mem_pestat] mean and std.dev: (4281.51, 2803.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1698, 3632, 6490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16074) [M::mem_pestat] mean and std.dev: (4180.95, 2827.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20866) [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1333334 reads in 514.548 CPU sec, 32.773 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7475, 129681, 6666, 7230) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1581, 3578, 6270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15648) [M::mem_pestat] mean and std.dev: (4066.31, 2829.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 328, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (330.37, 124.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1931, 3973, 6624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16010) [M::mem_pestat] mean and std.dev: (4332.77, 2799.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20703) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1602, 3655, 6381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15939) [M::mem_pestat] mean and std.dev: (4129.52, 2835.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20718) [M::mem_process_seqs] Processed 1333334 reads in 625.989 CPU sec, 40.727 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7519, 124491, 6891, 7315) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1600, 3552, 6222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15466) [M::mem_pestat] mean and std.dev: (4029.47, 2798.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20088) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 328, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (330.20, 124.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1872, 3908, 6616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16104) [M::mem_pestat] mean and std.dev: (4297.86, 2802.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20848) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1719, 3736, 6406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15780) [M::mem_pestat] mean and std.dev: (4183.75, 2823.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20467) [M::mem_process_seqs] Processed 1333334 reads in 655.704 CPU sec, 43.370 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7424, 129575, 6562, 7163) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1645, 3675, 6484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16162) [M::mem_pestat] mean and std.dev: (4155.83, 2832.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21001) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 328, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (331.06, 125.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1878, 3946, 6509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15771) [M::mem_pestat] mean and std.dev: (4290.80, 2774.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20402) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1636, 3604, 6317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15679) [M::mem_pestat] mean and std.dev: (4099.65, 2813.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20360) [M::mem_process_seqs] Processed 1333334 reads in 520.662 CPU sec, 33.031 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7584, 118680, 6879, 7362) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1536, 3608, 6343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15957) [M::mem_pestat] mean and std.dev: (4056.50, 2836.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 328, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (329.65, 124.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1860, 3970, 6577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16011) [M::mem_pestat] mean and std.dev: (4300.65, 2782.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20728) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1682, 3697, 6408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15860) [M::mem_pestat] mean and std.dev: (4169.93, 2816.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20586) [M::mem_process_seqs] Processed 1333334 reads in 630.663 CPU sec, 40.557 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7583, 127619, 6931, 7150) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1557, 3611, 6421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16149) [M::mem_pestat] mean and std.dev: (4096.00, 2854.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21013) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 329, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (331.51, 125.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1867, 3843, 6515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15811) [M::mem_pestat] mean and std.dev: (4271.17, 2792.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20459) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1663, 3682, 6386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15832) [M::mem_pestat] mean and std.dev: (4148.78, 2801.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20555) [M::mem_process_seqs] Processed 1333334 reads in 566.257 CPU sec, 36.307 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7656, 127217, 6574, 7326) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1620, 3602, 6342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15786) [M::mem_pestat] mean and std.dev: (4089.10, 2828.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 328, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (330.85, 125.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1842, 3866, 6607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16137) [M::mem_pestat] mean and std.dev: (4296.29, 2834.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20902) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1575, 3616, 6377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15981) [M::mem_pestat] mean and std.dev: (4088.51, 2810.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20783) [M::mem_process_seqs] Processed 1333334 reads in 573.142 CPU sec, 36.457 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7623, 128196, 6696, 7285) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1590, 3568, 6246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15558) [M::mem_pestat] mean and std.dev: (4038.47, 2805.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20214) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 328, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (330.68, 125.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1879, 3978, 6590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16012) [M::mem_pestat] mean and std.dev: (4337.11, 2819.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20723) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1685, 3749, 6452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15986) [M::mem_pestat] mean and std.dev: (4186.56, 2827.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20753) [M::mem_process_seqs] Processed 1333334 reads in 565.527 CPU sec, 35.885 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7399, 129285, 6752, 7277) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1610, 3637, 6436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16088) [M::mem_pestat] mean and std.dev: (4124.33, 2863.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20914) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 329, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (331.83, 126.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1836, 3898, 6527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15909) [M::mem_pestat] mean and std.dev: (4287.07, 2826.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20600) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1640, 3692, 6490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16190) [M::mem_pestat] mean and std.dev: (4174.33, 2837.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21040) [M::mem_process_seqs] Processed 1333334 reads in 541.596 CPU sec, 34.414 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7544, 130082, 6655, 7130) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1607, 3666, 6336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15794) [M::mem_pestat] mean and std.dev: (4106.80, 2833.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 329, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (332.01, 126.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1825, 3804, 6535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15955) [M::mem_pestat] mean and std.dev: (4239.35, 2793.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20665) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1667, 3743, 6434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15968) [M::mem_pestat] mean and std.dev: (4186.90, 2827.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20735) [M::mem_process_seqs] Processed 1333334 reads in 517.204 CPU sec, 33.056 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7501, 129688, 6874, 7120) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1563, 3684, 6440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16194) [M::mem_pestat] mean and std.dev: (4132.56, 2855.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21071) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 329, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (331.39, 125.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1853, 3912, 6553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15953) [M::mem_pestat] mean and std.dev: (4299.61, 2801.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1678, 3674, 6352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15700) [M::mem_pestat] mean and std.dev: (4134.75, 2799.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20374) [M::mem_process_seqs] Processed 1333334 reads in 536.250 CPU sec, 34.134 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7378, 130526, 6649, 7192) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1565, 3561, 6312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15806) [M::mem_pestat] mean and std.dev: (4064.94, 2826.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 330, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (332.65, 126.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1913, 3961, 6578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15908) [M::mem_pestat] mean and std.dev: (4329.02, 2792.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20573) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1611, 3563, 6319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15735) [M::mem_pestat] mean and std.dev: (4089.05, 2812.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20443) [M::mem_process_seqs] Processed 1333334 reads in 511.265 CPU sec, 32.670 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7351, 129363, 6805, 7172) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1572, 3560, 6319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15813) [M::mem_pestat] mean and std.dev: (4073.38, 2828.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (332.69, 126.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1915, 4034, 6685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16225) [M::mem_pestat] mean and std.dev: (4375.62, 2839.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20995) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1643, 3696, 6334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15716) [M::mem_pestat] mean and std.dev: (4135.17, 2807.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20407) [M::mem_process_seqs] Processed 1333334 reads in 532.774 CPU sec, 33.813 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7591, 129730, 6909, 7250) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1581, 3589, 6356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15906) [M::mem_pestat] mean and std.dev: (4078.94, 2826.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 329, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (331.74, 126.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1872, 3881, 6597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16047) [M::mem_pestat] mean and std.dev: (4289.55, 2796.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20772) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1669, 3723, 6423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15931) [M::mem_pestat] mean and std.dev: (4152.14, 2800.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20685) [M::mem_process_seqs] Processed 1333334 reads in 543.741 CPU sec, 34.604 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7437, 129483, 6803, 7276) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1568, 3575, 6309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15791) [M::mem_pestat] mean and std.dev: (4060.87, 2830.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 329, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (332.06, 125.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1846, 3933, 6686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16366) [M::mem_pestat] mean and std.dev: (4316.07, 2812.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21206) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1627, 3596, 6312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15682) [M::mem_pestat] mean and std.dev: (4088.38, 2809.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20367) [M::mem_process_seqs] Processed 1333334 reads in 535.160 CPU sec, 33.998 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7496, 130496, 6701, 7213) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1613, 3726, 6555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16439) [M::mem_pestat] mean and std.dev: (4185.03, 2870.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (333.28, 126.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1873, 3831, 6429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15541) [M::mem_pestat] mean and std.dev: (4230.11, 2755.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20097) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1681, 3634, 6408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15862) [M::mem_pestat] mean and std.dev: (4152.93, 2838.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20589) [M::mem_process_seqs] Processed 1333334 reads in 495.974 CPU sec, 31.479 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7578, 130030, 6611, 7188) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1639, 3733, 6439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16039) [M::mem_pestat] mean and std.dev: (4164.21, 2833.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20839) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (333.65, 126.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1801, 3782, 6489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15865) [M::mem_pestat] mean and std.dev: (4233.24, 2816.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20553) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1663, 3727, 6446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16012) [M::mem_pestat] mean and std.dev: (4171.42, 2830.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20795) [M::mem_process_seqs] Processed 1333334 reads in 481.496 CPU sec, 31.131 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7331, 131134, 6700, 7299) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1654, 3571, 6337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15703) [M::mem_pestat] mean and std.dev: (4093.15, 2831.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 330, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (332.21, 125.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1868, 3897, 6526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15842) [M::mem_pestat] mean and std.dev: (4274.83, 2790.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20500) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1654, 3685, 6375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15817) [M::mem_pestat] mean and std.dev: (4153.77, 2816.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20538) [M::mem_process_seqs] Processed 1333334 reads in 494.540 CPU sec, 31.472 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7398, 132537, 6630, 7235) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1557, 3615, 6397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16077) [M::mem_pestat] mean and std.dev: (4104.87, 2836.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 328, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (330.99, 125.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1908, 3926, 6516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15732) [M::mem_pestat] mean and std.dev: (4274.40, 2772.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20340) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1603, 3647, 6220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15454) [M::mem_pestat] mean and std.dev: (4074.40, 2798.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20071) [M::mem_process_seqs] Processed 1333334 reads in 496.748 CPU sec, 31.550 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7362, 133419, 6594, 7288) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1599, 3618, 6358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15876) [M::mem_pestat] mean and std.dev: (4095.97, 2837.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (238, 324, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (326.17, 125.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1860, 3931, 6573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15999) [M::mem_pestat] mean and std.dev: (4297.81, 2793.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20712) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1650, 3681, 6505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16215) [M::mem_pestat] mean and std.dev: (4177.64, 2850.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21070) [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1333334 reads in 446.534 CPU sec, 28.458 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7646, 131568, 6732, 7283) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1578, 3673, 6392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16020) [M::mem_pestat] mean and std.dev: (4095.52, 2818.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 327, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (330.31, 127.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1737, 3887, 6519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16083) [M::mem_pestat] mean and std.dev: (4215.61, 2790.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20865) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1604, 3655, 6401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15995) [M::mem_pestat] mean and std.dev: (4128.26, 2818.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20792) [M::mem_process_seqs] Processed 1333334 reads in 419.003 CPU sec, 26.681 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7300, 132565, 6661, 7337) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1602, 3571, 6252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15552) [M::mem_pestat] mean and std.dev: (4045.89, 2799.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 328, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (331.18, 127.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1886, 3930, 6500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15728) [M::mem_pestat] mean and std.dev: (4281.02, 2763.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20342) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1721, 3781, 6385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15713) [M::mem_pestat] mean and std.dev: (4192.57, 2801.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20377) [M::mem_process_seqs] Processed 1333334 reads in 399.065 CPU sec, 25.401 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7484, 131488, 6696, 7162) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1549, 3705, 6524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16474) [M::mem_pestat] mean and std.dev: (4139.72, 2864.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 330, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (332.24, 127.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1783, 3882, 6468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15838) [M::mem_pestat] mean and std.dev: (4233.50, 2794.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20523) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1669, 3714, 6422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15928) [M::mem_pestat] mean and std.dev: (4166.09, 2828.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20681) [M::mem_process_seqs] Processed 1333334 reads in 399.389 CPU sec, 25.432 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7521, 130878, 6701, 7204) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1569, 3673, 6372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15978) [M::mem_pestat] mean and std.dev: (4108.52, 2849.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (332.86, 127.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1753, 3815, 6533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16093) [M::mem_pestat] mean and std.dev: (4244.28, 2835.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20873) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1662, 3727, 6591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16449) [M::mem_pestat] mean and std.dev: (4197.31, 2855.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21378) [M::mem_process_seqs] Processed 1333334 reads in 395.008 CPU sec, 25.201 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7605, 131427, 6697, 7347) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1657, 3708, 6473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16105) [M::mem_pestat] mean and std.dev: (4161.80, 2825.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 330, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (333.20, 128.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1807, 3913, 6528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15970) [M::mem_pestat] mean and std.dev: (4270.38, 2827.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20691) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1635, 3722, 6477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16161) [M::mem_pestat] mean and std.dev: (4163.88, 2848.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21003) [M::mem_process_seqs] Processed 1333334 reads in 387.598 CPU sec, 24.989 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7594, 131228, 6820, 7348) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1577, 3702, 6369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15953) [M::mem_pestat] mean and std.dev: (4120.32, 2847.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 330, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (332.52, 127.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1845, 3949, 6609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16137) [M::mem_pestat] mean and std.dev: (4332.38, 2826.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20901) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1690, 3741, 6389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15787) [M::mem_pestat] mean and std.dev: (4176.66, 2803.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20486) [M::mem_process_seqs] Processed 1333334 reads in 397.640 CPU sec, 25.378 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7410, 131376, 6706, 7243) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1601, 3676, 6409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16025) [M::mem_pestat] mean and std.dev: (4121.49, 2837.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 331, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (333.44, 128.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1897, 3915, 6459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15583) [M::mem_pestat] mean and std.dev: (4270.57, 2762.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20145) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1626, 3744, 6465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16143) [M::mem_pestat] mean and std.dev: (4160.34, 2837.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20982) [M::mem_process_seqs] Processed 1333334 reads in 389.065 CPU sec, 24.752 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7502, 130997, 6794, 7307) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1653, 3720, 6337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15705) [M::mem_pestat] mean and std.dev: (4131.67, 2798.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 329, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (332.39, 127.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1838, 3820, 6504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15836) [M::mem_pestat] mean and std.dev: (4258.04, 2814.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20502) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1741, 3712, 6324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15490) [M::mem_pestat] mean and std.dev: (4137.49, 2769.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20073) [M::mem_process_seqs] Processed 1333334 reads in 398.877 CPU sec, 25.591 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7375, 130529, 6780, 7284) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1595, 3633, 6257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15581) [M::mem_pestat] mean and std.dev: (4074.34, 2806.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20243) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 330, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (332.51, 127.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1859, 3899, 6665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16277) [M::mem_pestat] mean and std.dev: (4322.11, 2843.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21083) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1637, 3678, 6406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15944) [M::mem_pestat] mean and std.dev: (4149.98, 2831.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20713) [M::mem_process_seqs] Processed 1333334 reads in 412.250 CPU sec, 26.236 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7352, 130142, 6679, 7395) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1548, 3567, 6331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15897) [M::mem_pestat] mean and std.dev: (4064.02, 2834.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20680) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 330, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (333.02, 127.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1811, 3847, 6465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15773) [M::mem_pestat] mean and std.dev: (4242.19, 2802.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20427) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1665, 3695, 6428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15954) [M::mem_pestat] mean and std.dev: (4172.34, 2832.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20717) [M::mem_process_seqs] Processed 1333334 reads in 411.178 CPU sec, 26.121 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7442, 128470, 6865, 7346) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1603, 3696, 6380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15934) [M::mem_pestat] mean and std.dev: (4121.10, 2827.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 329, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (331.38, 126.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1819, 3911, 6649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16309) [M::mem_pestat] mean and std.dev: (4314.94, 2824.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21139) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1658, 3638, 6295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15569) [M::mem_pestat] mean and std.dev: (4101.26, 2797.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20206) [M::mem_process_seqs] Processed 1333334 reads in 448.911 CPU sec, 28.581 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7481, 130645, 6704, 7253) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1584, 3728, 6464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16224) [M::mem_pestat] mean and std.dev: (4144.96, 2863.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21104) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 332, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (334.53, 128.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1794, 3828, 6478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15846) [M::mem_pestat] mean and std.dev: (4232.01, 2806.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20530) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1662, 3609, 6302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15582) [M::mem_pestat] mean and std.dev: (4101.29, 2822.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20222) [M::mem_process_seqs] Processed 1333334 reads in 389.829 CPU sec, 24.757 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7462, 130682, 6744, 7304) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1608, 3575, 6261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15567) [M::mem_pestat] mean and std.dev: (4052.54, 2791.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 332, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (334.39, 128.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1914, 3970, 6735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16377) [M::mem_pestat] mean and std.dev: (4371.26, 2832.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21198) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1682, 3663, 6355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15701) [M::mem_pestat] mean and std.dev: (4122.92, 2804.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20374) [M::mem_process_seqs] Processed 1333334 reads in 389.540 CPU sec, 24.792 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7410, 130267, 6645, 7407) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1627, 3663, 6369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15853) [M::mem_pestat] mean and std.dev: (4124.04, 2835.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (334.44, 127.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1859, 3976, 6583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16031) [M::mem_pestat] mean and std.dev: (4314.46, 2806.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20755) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1656, 3677, 6333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15687) [M::mem_pestat] mean and std.dev: (4109.31, 2794.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20364) [M::mem_process_seqs] Processed 1333334 reads in 398.936 CPU sec, 25.423 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7386, 130881, 6734, 7297) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1608, 3702, 6530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16374) [M::mem_pestat] mean and std.dev: (4173.91, 2863.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21296) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 331, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (333.59, 128.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1877, 4017, 6631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16139) [M::mem_pestat] mean and std.dev: (4323.75, 2801.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20893) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1621, 3671, 6380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15898) [M::mem_pestat] mean and std.dev: (4127.70, 2804.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20657) [M::mem_process_seqs] Processed 1333334 reads in 396.139 CPU sec, 25.260 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7473, 129178, 6733, 7295) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1556, 3633, 6392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16064) [M::mem_pestat] mean and std.dev: (4094.24, 2843.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (334.57, 128.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1923, 3971, 6682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16200) [M::mem_pestat] mean and std.dev: (4360.40, 2815.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20959) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1596, 3621, 6322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15774) [M::mem_pestat] mean and std.dev: (4093.19, 2804.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20500) [M::mem_process_seqs] Processed 1333334 reads in 409.663 CPU sec, 26.005 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7485, 127642, 6896, 7346) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1591, 3650, 6265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15613) [M::mem_pestat] mean and std.dev: (4077.28, 2800.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20287) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 329, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (331.75, 126.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1803, 3790, 6473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15813) [M::mem_pestat] mean and std.dev: (4206.76, 2783.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20483) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1620, 3613, 6380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15900) [M::mem_pestat] mean and std.dev: (4104.32, 2815.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20660) [M::mem_process_seqs] Processed 1333334 reads in 458.468 CPU sec, 29.139 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7424, 129605, 6641, 7183) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1658, 3747, 6402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15890) [M::mem_pestat] mean and std.dev: (4156.14, 2828.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 332, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (335.25, 128.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1717, 3745, 6555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16231) [M::mem_pestat] mean and std.dev: (4195.87, 2807.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21069) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1609, 3665, 6246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15520) [M::mem_pestat] mean and std.dev: (4090.28, 2787.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20157) [M::mem_process_seqs] Processed 1333334 reads in 392.423 CPU sec, 24.994 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7437, 130215, 6778, 7202) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1657, 3689, 6427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15967) [M::mem_pestat] mean and std.dev: (4163.56, 2817.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 333, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (335.23, 129.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1861, 3876, 6650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16228) [M::mem_pestat] mean and std.dev: (4283.65, 2824.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21017) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1691, 3671, 6312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15554) [M::mem_pestat] mean and std.dev: (4122.74, 2796.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20175) [M::mem_process_seqs] Processed 1333334 reads in 399.898 CPU sec, 25.466 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7406, 129759, 6771, 7399) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1591, 3721, 6434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16120) [M::mem_pestat] mean and std.dev: (4148.80, 2846.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 332, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (334.90, 129.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1841, 3950, 6664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16310) [M::mem_pestat] mean and std.dev: (4323.45, 2811.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21133) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1639, 3741, 6402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15928) [M::mem_pestat] mean and std.dev: (4159.31, 2817.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20691) [M::mem_process_seqs] Processed 1333334 reads in 390.179 CPU sec, 24.776 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7498, 130052, 6807, 7362) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1621, 3664, 6275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15583) [M::mem_pestat] mean and std.dev: (4087.88, 2794.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20237) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 332, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (335.60, 129.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1832, 3982, 6608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16160) [M::mem_pestat] mean and std.dev: (4325.17, 2823.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20936) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1603, 3577, 6308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15718) [M::mem_pestat] mean and std.dev: (4067.66, 2791.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20423) [M::mem_process_seqs] Processed 1333334 reads in 389.741 CPU sec, 24.888 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7591, 129356, 6683, 7470) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1639, 3577, 6321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15685) [M::mem_pestat] mean and std.dev: (4090.97, 2809.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 334, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (336.22, 129.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1829, 3830, 6583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16091) [M::mem_pestat] mean and std.dev: (4275.32, 2831.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20845) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1640, 3690, 6412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15956) [M::mem_pestat] mean and std.dev: (4163.48, 2840.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20728) [M::mem_process_seqs] Processed 1333334 reads in 388.260 CPU sec, 24.818 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7384, 129014, 6822, 7175) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1542, 3702, 6457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16287) [M::mem_pestat] mean and std.dev: (4158.37, 2872.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 334, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (336.28, 129.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1860, 3963, 6627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16161) [M::mem_pestat] mean and std.dev: (4327.86, 2834.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20928) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1655, 3606, 6310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15620) [M::mem_pestat] mean and std.dev: (4116.28, 2813.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20275) [M::mem_process_seqs] Processed 1333334 reads in 390.855 CPU sec, 24.878 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7478, 129589, 6675, 7226) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1609, 3563, 6286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15640) [M::mem_pestat] mean and std.dev: (4074.43, 2809.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20317) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 334, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (336.41, 129.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1845, 3835, 6545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15945) [M::mem_pestat] mean and std.dev: (4269.40, 2795.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20645) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1659, 3682, 6405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15897) [M::mem_pestat] mean and std.dev: (4137.81, 2826.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20643) [M::mem_process_seqs] Processed 1333334 reads in 386.657 CPU sec, 25.032 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7454, 129680, 6751, 7219) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1643, 3737, 6466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16112) [M::mem_pestat] mean and std.dev: (4191.02, 2867.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 334, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (336.93, 130.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1848, 3934, 6555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15969) [M::mem_pestat] mean and std.dev: (4283.89, 2811.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20676) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1739, 3765, 6410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15752) [M::mem_pestat] mean and std.dev: (4173.32, 2788.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20423) [M::mem_process_seqs] Processed 1333334 reads in 388.059 CPU sec, 24.694 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7543, 129680, 6565, 7272) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1622, 3690, 6346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15794) [M::mem_pestat] mean and std.dev: (4120.77, 2813.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 331, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (333.52, 128.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1840, 3963, 6699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16417) [M::mem_pestat] mean and std.dev: (4308.80, 2819.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21276) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1636, 3696, 6391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15901) [M::mem_pestat] mean and std.dev: (4141.85, 2804.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20656) [M::mem_process_seqs] Processed 1333334 reads in 439.960 CPU sec, 28.081 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7508, 129059, 6721, 7568) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1572, 3697, 6348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15900) [M::mem_pestat] mean and std.dev: (4122.86, 2849.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 334, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (336.76, 130.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1824, 3837, 6570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16062) [M::mem_pestat] mean and std.dev: (4244.69, 2811.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20808) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1664, 3698, 6369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15779) [M::mem_pestat] mean and std.dev: (4137.70, 2809.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20484) [M::mem_process_seqs] Processed 1333334 reads in 389.940 CPU sec, 24.778 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7403, 128697, 6677, 7317) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1620, 3585, 6402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15966) [M::mem_pestat] mean and std.dev: (4110.60, 2839.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 335, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (337.73, 130.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1794, 3918, 6566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16110) [M::mem_pestat] mean and std.dev: (4289.66, 2807.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20882) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1677, 3671, 6445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15981) [M::mem_pestat] mean and std.dev: (4168.36, 2828.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20749) [M::mem_process_seqs] Processed 1333334 reads in 394.026 CPU sec, 25.114 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7468, 129494, 6702, 7390) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1647, 3721, 6438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16020) [M::mem_pestat] mean and std.dev: (4162.38, 2834.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 334, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (336.29, 130.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1828, 3947, 6651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16297) [M::mem_pestat] mean and std.dev: (4301.92, 2822.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21120) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1669, 3789, 6457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16033) [M::mem_pestat] mean and std.dev: (4183.51, 2829.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20821) [M::mem_process_seqs] Processed 1333334 reads in 387.643 CPU sec, 24.747 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7563, 129039, 6701, 7363) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1641, 3757, 6466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16116) [M::mem_pestat] mean and std.dev: (4175.84, 2838.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20941) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 336, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (338.18, 130.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1830, 3835, 6600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16140) [M::mem_pestat] mean and std.dev: (4268.27, 2817.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20910) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1645, 3623, 6277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15541) [M::mem_pestat] mean and std.dev: (4108.51, 2809.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20173) [M::mem_process_seqs] Processed 1333334 reads in 395.352 CPU sec, 25.121 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7502, 128196, 6839, 7354) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1629, 3640, 6351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15795) [M::mem_pestat] mean and std.dev: (4096.33, 2802.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 334, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (337.27, 130.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1890, 3963, 6571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15933) [M::mem_pestat] mean and std.dev: (4336.83, 2802.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20614) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1604, 3644, 6353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15851) [M::mem_pestat] mean and std.dev: (4098.70, 2807.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20600) [M::mem_process_seqs] Processed 1333334 reads in 397.533 CPU sec, 25.378 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7568, 128006, 6779, 7267) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1645, 3627, 6338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15724) [M::mem_pestat] mean and std.dev: (4116.10, 2813.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 336, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (338.15, 129.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1866, 3945, 6668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16272) [M::mem_pestat] mean and std.dev: (4317.81, 2810.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21074) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1595, 3713, 6427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16091) [M::mem_pestat] mean and std.dev: (4161.37, 2840.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20923) [M::mem_process_seqs] Processed 1333334 reads in 411.249 CPU sec, 26.234 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7394, 128367, 6782, 7119) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1655, 3700, 6280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15530) [M::mem_pestat] mean and std.dev: (4113.67, 2800.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20155) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 335, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (338.25, 130.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1911, 3925, 6593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15957) [M::mem_pestat] mean and std.dev: (4315.69, 2787.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20639) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1593, 3670, 6377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15945) [M::mem_pestat] mean and std.dev: (4121.51, 2819.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20729) [M::mem_process_seqs] Processed 1333334 reads in 397.397 CPU sec, 25.324 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7452, 128492, 6855, 7227) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1662, 3693, 6422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15942) [M::mem_pestat] mean and std.dev: (4153.05, 2834.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 336, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (338.03, 130.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1845, 3907, 6588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16074) [M::mem_pestat] mean and std.dev: (4278.81, 2799.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20817) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1622, 3596, 6245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15491) [M::mem_pestat] mean and std.dev: (4079.27, 2799.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20114) [M::mem_process_seqs] Processed 1333334 reads in 391.717 CPU sec, 24.960 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7378, 128636, 6694, 7345) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1615, 3663, 6384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15922) [M::mem_pestat] mean and std.dev: (4122.67, 2840.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 334, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (337.09, 130.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1895, 3999, 6659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16187) [M::mem_pestat] mean and std.dev: (4355.04, 2817.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20951) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1626, 3759, 6369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15855) [M::mem_pestat] mean and std.dev: (4165.52, 2817.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20598) [M::mem_process_seqs] Processed 1333334 reads in 393.474 CPU sec, 25.017 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7574, 127873, 6856, 7325) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1602, 3643, 6283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15645) [M::mem_pestat] mean and std.dev: (4071.94, 2806.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20326) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 335, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (338.09, 130.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1881, 3933, 6499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15735) [M::mem_pestat] mean and std.dev: (4277.80, 2787.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20353) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1666, 3647, 6402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15874) [M::mem_pestat] mean and std.dev: (4151.21, 2813.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20610) [M::mem_process_seqs] Processed 1333334 reads in 389.627 CPU sec, 24.881 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7551, 127254, 6656, 7443) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1711, 3714, 6511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16111) [M::mem_pestat] mean and std.dev: (4180.31, 2831.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 337, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (339.15, 130.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1867, 3912, 6582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16012) [M::mem_pestat] mean and std.dev: (4312.60, 2821.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20727) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1654, 3698, 6313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15631) [M::mem_pestat] mean and std.dev: (4136.20, 2793.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20290) [M::mem_process_seqs] Processed 1333334 reads in 395.112 CPU sec, 25.258 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7330, 128248, 6822, 7422) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1669, 3660, 6291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15535) [M::mem_pestat] mean and std.dev: (4110.37, 2793.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20157) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 336, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (339.32, 130.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1913, 3916, 6487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15635) [M::mem_pestat] mean and std.dev: (4292.27, 2757.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20209) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1645, 3653, 6418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15964) [M::mem_pestat] mean and std.dev: (4120.66, 2821.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20737) [M::mem_process_seqs] Processed 1333334 reads in 394.454 CPU sec, 25.060 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7309, 127743, 6717, 7275) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1579, 3559, 6291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15715) [M::mem_pestat] mean and std.dev: (4066.14, 2824.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 336, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (338.53, 130.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1840, 3822, 6552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15976) [M::mem_pestat] mean and std.dev: (4254.00, 2816.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20688) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1670, 3700, 6475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16085) [M::mem_pestat] mean and std.dev: (4177.32, 2834.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20890) [M::mem_process_seqs] Processed 1333334 reads in 394.046 CPU sec, 25.133 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7564, 128352, 6754, 7158) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1683, 3789, 6506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16152) [M::mem_pestat] mean and std.dev: (4201.75, 2832.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 336, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (339.09, 131.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1871, 3850, 6503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15767) [M::mem_pestat] mean and std.dev: (4257.89, 2793.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20399) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1700, 3789, 6539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16217) [M::mem_pestat] mean and std.dev: (4216.45, 2844.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21056) [M::mem_process_seqs] Processed 1333334 reads in 390.585 CPU sec, 24.899 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7561, 127330, 6664, 7472) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1628, 3665, 6329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15731) [M::mem_pestat] mean and std.dev: (4112.97, 2814.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 337, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (339.39, 131.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1811, 3917, 6525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15953) [M::mem_pestat] mean and std.dev: (4269.84, 2808.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20667) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1611, 3561, 6367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15879) [M::mem_pestat] mean and std.dev: (4115.02, 2817.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20635) [M::mem_process_seqs] Processed 1333334 reads in 390.879 CPU sec, 24.886 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7374, 127361, 6814, 7431) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1603, 3550, 6244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15526) [M::mem_pestat] mean and std.dev: (4067.10, 2819.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20167) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 338, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (340.51, 132.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1834, 3912, 6512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15868) [M::mem_pestat] mean and std.dev: (4268.36, 2809.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20546) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1668, 3692, 6416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15912) [M::mem_pestat] mean and std.dev: (4168.63, 2826.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20660) [M::mem_process_seqs] Processed 1333334 reads in 390.269 CPU sec, 24.884 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7470, 128409, 6727, 7416) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1589, 3695, 6385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15977) [M::mem_pestat] mean and std.dev: (4123.46, 2823.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 338, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (340.63, 131.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1821, 3934, 6563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16047) [M::mem_pestat] mean and std.dev: (4277.61, 2806.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20789) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1649, 3630, 6412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15938) [M::mem_pestat] mean and std.dev: (4130.17, 2818.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20701) [M::mem_process_seqs] Processed 1333334 reads in 387.708 CPU sec, 24.689 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7436, 127223, 6850, 7259) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1557, 3656, 6373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16005) [M::mem_pestat] mean and std.dev: (4103.82, 2827.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 336, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (338.49, 130.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1841, 3930, 6520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15878) [M::mem_pestat] mean and std.dev: (4282.44, 2796.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20557) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1704, 3813, 6410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15822) [M::mem_pestat] mean and std.dev: (4199.30, 2798.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20528) [M::mem_process_seqs] Processed 1333334 reads in 426.085 CPU sec, 27.165 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7535, 128011, 6783, 7219) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1602, 3663, 6424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16068) [M::mem_pestat] mean and std.dev: (4122.03, 2843.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20890) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 338, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (339.76, 131.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1825, 3813, 6582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16096) [M::mem_pestat] mean and std.dev: (4261.84, 2808.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20853) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1677, 3738, 6399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15843) [M::mem_pestat] mean and std.dev: (4175.73, 2822.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20565) [M::mem_process_seqs] Processed 1333334 reads in 385.307 CPU sec, 24.836 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7540, 127655, 6907, 7391) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1530, 3534, 6322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15906) [M::mem_pestat] mean and std.dev: (4042.79, 2830.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 338, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (340.65, 131.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1868, 3910, 6604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16076) [M::mem_pestat] mean and std.dev: (4301.61, 2803.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20812) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1711, 3741, 6400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15778) [M::mem_pestat] mean and std.dev: (4175.13, 2802.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20467) [M::mem_process_seqs] Processed 1333334 reads in 387.401 CPU sec, 24.720 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7440, 127677, 6858, 7361) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1660, 3602, 6357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15751) [M::mem_pestat] mean and std.dev: (4099.16, 2820.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 339, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (340.81, 132.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1830, 3830, 6523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15909) [M::mem_pestat] mean and std.dev: (4271.74, 2824.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20602) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1647, 3603, 6302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15612) [M::mem_pestat] mean and std.dev: (4108.46, 2802.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20267) [M::mem_process_seqs] Processed 1333334 reads in 384.295 CPU sec, 24.468 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7524, 128170, 6884, 7350) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1636, 3614, 6452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16084) [M::mem_pestat] mean and std.dev: (4145.37, 2836.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 339, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (341.18, 131.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1846, 3946, 6576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16036) [M::mem_pestat] mean and std.dev: (4314.86, 2805.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20766) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1663, 3722, 6412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15910) [M::mem_pestat] mean and std.dev: (4182.07, 2827.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20659) [M::mem_process_seqs] Processed 1333334 reads in 395.024 CPU sec, 25.159 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7578, 126984, 6854, 7288) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1613, 3660, 6333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15773) [M::mem_pestat] mean and std.dev: (4093.41, 2799.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 338, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (340.91, 130.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1831, 3883, 6446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15676) [M::mem_pestat] mean and std.dev: (4258.54, 2786.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20291) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1652, 3669, 6397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15887) [M::mem_pestat] mean and std.dev: (4142.62, 2799.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20632) [M::mem_process_seqs] Processed 1333334 reads in 386.168 CPU sec, 24.533 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7616, 127647, 6928, 7461) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1680, 3747, 6335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15645) [M::mem_pestat] mean and std.dev: (4135.18, 2806.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20300) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 338, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (340.02, 131.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1870, 3941, 6668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16264) [M::mem_pestat] mean and std.dev: (4315.41, 2814.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21062) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1687, 3736, 6433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15925) [M::mem_pestat] mean and std.dev: (4181.72, 2807.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20671) [M::mem_process_seqs] Processed 1333334 reads in 392.485 CPU sec, 25.228 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7555, 126641, 6791, 7399) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1549, 3641, 6407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16123) [M::mem_pestat] mean and std.dev: (4126.09, 2876.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 339, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (340.73, 131.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1825, 3912, 6613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16189) [M::mem_pestat] mean and std.dev: (4298.84, 2832.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20977) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1683, 3686, 6291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15507) [M::mem_pestat] mean and std.dev: (4134.01, 2789.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20115) [M::mem_process_seqs] Processed 1333334 reads in 400.685 CPU sec, 25.545 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7356, 129707, 6682, 7236) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1624, 3592, 6406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15970) [M::mem_pestat] mean and std.dev: (4110.21, 2824.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 331, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (333.79, 129.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1950, 4009, 6711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16233) [M::mem_pestat] mean and std.dev: (4370.13, 2812.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20994) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1656, 3693, 6407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15909) [M::mem_pestat] mean and std.dev: (4142.01, 2817.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20660) [M::mem_process_seqs] Processed 1333334 reads in 414.413 CPU sec, 26.325 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7482, 132892, 6611, 7143) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1556, 3656, 6424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16160) [M::mem_pestat] mean and std.dev: (4093.60, 2849.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21028) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (241, 326, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (329.23, 126.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1900, 3995, 6634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16102) [M::mem_pestat] mean and std.dev: (4351.78, 2824.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20836) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1709, 3682, 6408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15806) [M::mem_pestat] mean and std.dev: (4160.18, 2808.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20505) [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1333334 reads in 433.812 CPU sec, 27.578 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7394, 130036, 6672, 7398) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1602, 3622, 6398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15990) [M::mem_pestat] mean and std.dev: (4103.57, 2851.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (241, 326, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (329.38, 126.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1895, 3984, 6689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16277) [M::mem_pestat] mean and std.dev: (4356.69, 2821.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21071) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1663, 3753, 6595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16459) [M::mem_pestat] mean and std.dev: (4197.83, 2850.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21391) [M::mem_process_seqs] Processed 1333334 reads in 456.365 CPU sec, 29.115 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7525, 131773, 6766, 7173) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1561, 3608, 6382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16024) [M::mem_pestat] mean and std.dev: (4094.13, 2855.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 329, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (331.75, 127.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1830, 3841, 6528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15924) [M::mem_pestat] mean and std.dev: (4242.88, 2798.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20622) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1683, 3771, 6454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15996) [M::mem_pestat] mean and std.dev: (4188.18, 2808.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20767) [M::mem_process_seqs] Processed 1333334 reads in 396.689 CPU sec, 25.444 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7512, 131302, 6750, 7399) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1657, 3619, 6287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15547) [M::mem_pestat] mean and std.dev: (4086.61, 2806.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20177) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 330, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (332.49, 128.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1872, 3940, 6528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15840) [M::mem_pestat] mean and std.dev: (4297.03, 2778.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20496) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1577, 3577, 6243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15575) [M::mem_pestat] mean and std.dev: (4055.65, 2797.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20241) [M::mem_process_seqs] Processed 1333334 reads in 402.483 CPU sec, 25.714 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7428, 131525, 6744, 7361) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1688, 3715, 6374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15746) [M::mem_pestat] mean and std.dev: (4135.90, 2796.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 330, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (332.90, 128.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1822, 3964, 6626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16234) [M::mem_pestat] mean and std.dev: (4312.73, 2818.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21038) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1651, 3713, 6411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15931) [M::mem_pestat] mean and std.dev: (4163.60, 2824.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20691) [M::mem_process_seqs] Processed 1333334 reads in 387.266 CPU sec, 24.864 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7415, 130198, 6910, 7376) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1581, 3581, 6346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15876) [M::mem_pestat] mean and std.dev: (4084.68, 2820.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 331, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (333.34, 127.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1811, 3956, 6538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15992) [M::mem_pestat] mean and std.dev: (4306.13, 2819.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20719) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1583, 3706, 6433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16133) [M::mem_pestat] mean and std.dev: (4125.88, 2825.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20983) [M::mem_process_seqs] Processed 1333334 reads in 411.437 CPU sec, 26.195 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7612, 130908, 6773, 7230) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1679, 3739, 6413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15881) [M::mem_pestat] mean and std.dev: (4153.76, 2806.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 330, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (333.20, 128.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1866, 3874, 6482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15714) [M::mem_pestat] mean and std.dev: (4267.21, 2785.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20330) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1678, 3727, 6339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15661) [M::mem_pestat] mean and std.dev: (4133.82, 2798.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20322) [M::mem_process_seqs] Processed 1333334 reads in 399.320 CPU sec, 25.415 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7547, 131138, 6911, 7265) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1617, 3666, 6356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15834) [M::mem_pestat] mean and std.dev: (4117.38, 2821.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 330, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (332.93, 127.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1833, 3834, 6468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15738) [M::mem_pestat] mean and std.dev: (4238.21, 2788.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20373) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1658, 3766, 6484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16136) [M::mem_pestat] mean and std.dev: (4198.81, 2830.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20962) [M::mem_process_seqs] Processed 1333334 reads in 391.745 CPU sec, 24.955 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7514, 131445, 6890, 7324) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1571, 3693, 6406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16076) [M::mem_pestat] mean and std.dev: (4119.56, 2841.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 329, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (331.84, 127.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1797, 3823, 6398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15600) [M::mem_pestat] mean and std.dev: (4203.57, 2779.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20201) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1692, 3728, 6367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15717) [M::mem_pestat] mean and std.dev: (4168.48, 2788.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20392) [M::mem_process_seqs] Processed 1333334 reads in 399.135 CPU sec, 25.493 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7529, 130867, 6732, 7316) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1642, 3641, 6350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15766) [M::mem_pestat] mean and std.dev: (4101.82, 2801.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 330, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (332.44, 127.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1873, 3979, 6759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16531) [M::mem_pestat] mean and std.dev: (4361.25, 2837.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21417) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1654, 3596, 6272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15508) [M::mem_pestat] mean and std.dev: (4089.16, 2797.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20126) [M::mem_process_seqs] Processed 1333334 reads in 401.036 CPU sec, 25.600 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7396, 129026, 6812, 7301) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1659, 3692, 6312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15618) [M::mem_pestat] mean and std.dev: (4129.69, 2801.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20271) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 330, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (332.57, 127.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1835, 3918, 6553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15989) [M::mem_pestat] mean and std.dev: (4264.07, 2797.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20707) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1609, 3731, 6380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15922) [M::mem_pestat] mean and std.dev: (4148.23, 2831.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20693) [M::mem_process_seqs] Processed 1333334 reads in 429.474 CPU sec, 27.416 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7494, 127728, 6727, 7275) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1576, 3733, 6309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15775) [M::mem_pestat] mean and std.dev: (4116.14, 2817.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 330, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (332.01, 128.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1841, 3854, 6653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16277) [M::mem_pestat] mean and std.dev: (4297.50, 2849.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21089) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1682, 3640, 6391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15809) [M::mem_pestat] mean and std.dev: (4139.21, 2809.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20518) [M::mem_process_seqs] Processed 1333334 reads in 446.577 CPU sec, 28.381 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7564, 130379, 6621, 7375) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1626, 3724, 6438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16062) [M::mem_pestat] mean and std.dev: (4164.68, 2840.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 331, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (333.90, 128.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1804, 3828, 6542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16018) [M::mem_pestat] mean and std.dev: (4262.62, 2812.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20756) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1617, 3685, 6430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16056) [M::mem_pestat] mean and std.dev: (4154.28, 2841.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20869) [M::mem_process_seqs] Processed 1333334 reads in 397.068 CPU sec, 25.501 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7635, 130604, 6801, 7190) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1592, 3578, 6284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15668) [M::mem_pestat] mean and std.dev: (4056.72, 2806.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 332, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (334.38, 128.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1866, 3956, 6659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16245) [M::mem_pestat] mean and std.dev: (4318.07, 2811.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21038) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1675, 3734, 6380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15790) [M::mem_pestat] mean and std.dev: (4168.04, 2819.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20495) [M::mem_process_seqs] Processed 1333334 reads in 390.673 CPU sec, 24.986 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7487, 129346, 6689, 7256) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1635, 3724, 6446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16068) [M::mem_pestat] mean and std.dev: (4166.64, 2838.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (333.93, 128.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1875, 3895, 6552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15906) [M::mem_pestat] mean and std.dev: (4282.50, 2801.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20583) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1654, 3674, 6370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15802) [M::mem_pestat] mean and std.dev: (4150.80, 2832.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20518) [M::mem_process_seqs] Processed 1333334 reads in 405.812 CPU sec, 25.894 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7493, 129217, 6747, 6985) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1617, 3575, 6244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15498) [M::mem_pestat] mean and std.dev: (4069.98, 2805.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20125) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 332, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (335.21, 129.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1848, 3866, 6485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15759) [M::mem_pestat] mean and std.dev: (4257.51, 2802.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20396) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1659, 3683, 6407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15903) [M::mem_pestat] mean and std.dev: (4134.22, 2809.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20651) [M::mem_process_seqs] Processed 1333334 reads in 403.615 CPU sec, 25.648 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7531, 124808, 6585, 7279) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1575, 3630, 6320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15810) [M::mem_pestat] mean and std.dev: (4098.62, 2844.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 328, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (331.33, 127.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1007) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1769, 3847, 6545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16097) [M::mem_pestat] mean and std.dev: (4235.01, 2803.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20873) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1631, 3620, 6318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15692) [M::mem_pestat] mean and std.dev: (4105.53, 2804.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20379) [M::mem_process_seqs] Processed 1333334 reads in 481.705 CPU sec, 30.782 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7447, 130801, 6818, 7225) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1587, 3694, 6427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16107) [M::mem_pestat] mean and std.dev: (4101.62, 2831.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 329, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (332.76, 128.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1892, 3928, 6569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15923) [M::mem_pestat] mean and std.dev: (4294.30, 2804.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20600) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1609, 3630, 6440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16102) [M::mem_pestat] mean and std.dev: (4128.93, 2833.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20933) [M::mem_process_seqs] Processed 1333334 reads in 391.730 CPU sec, 25.118 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7381, 129181, 6669, 7411) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1632, 3584, 6269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15543) [M::mem_pestat] mean and std.dev: (4066.41, 2810.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20180) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 332, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (335.34, 128.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1829, 3840, 6525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15917) [M::mem_pestat] mean and std.dev: (4246.98, 2794.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20613) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1650, 3697, 6428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15984) [M::mem_pestat] mean and std.dev: (4161.84, 2826.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20762) [M::mem_process_seqs] Processed 1333334 reads in 399.642 CPU sec, 25.562 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7576, 129663, 6876, 7287) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1634, 3715, 6409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15959) [M::mem_pestat] mean and std.dev: (4149.96, 2831.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 333, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (335.35, 128.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1865, 3943, 6568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15974) [M::mem_pestat] mean and std.dev: (4293.87, 2804.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20677) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1710, 3738, 6501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16083) [M::mem_pestat] mean and std.dev: (4204.64, 2829.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20874) [M::mem_process_seqs] Processed 1333334 reads in 393.402 CPU sec, 25.124 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7392, 129951, 6782, 7333) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1616, 3683, 6378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15902) [M::mem_pestat] mean and std.dev: (4120.53, 2825.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 333, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (335.39, 129.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1882, 3928, 6537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15847) [M::mem_pestat] mean and std.dev: (4303.93, 2812.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20502) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1657, 3651, 6405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15901) [M::mem_pestat] mean and std.dev: (4137.36, 2804.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20649) [M::mem_process_seqs] Processed 1333334 reads in 389.098 CPU sec, 24.714 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7413, 129634, 6736, 7289) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1654, 3662, 6381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15835) [M::mem_pestat] mean and std.dev: (4117.36, 2818.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 335, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (337.14, 129.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1849, 3906, 6459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15679) [M::mem_pestat] mean and std.dev: (4256.01, 2776.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20289) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1681, 3752, 6429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15925) [M::mem_pestat] mean and std.dev: (4182.19, 2820.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20673) [M::mem_process_seqs] Processed 1333334 reads in 389.637 CPU sec, 24.877 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7490, 128845, 6888, 7403) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1633, 3638, 6415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15979) [M::mem_pestat] mean and std.dev: (4108.29, 2815.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 334, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (336.79, 130.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1813, 3915, 6605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16189) [M::mem_pestat] mean and std.dev: (4305.99, 2822.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20981) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1637, 3691, 6377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15857) [M::mem_pestat] mean and std.dev: (4152.63, 2834.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20597) [M::mem_process_seqs] Processed 1333334 reads in 391.924 CPU sec, 24.951 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7543, 128769, 6727, 7206) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1539, 3650, 6357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15993) [M::mem_pestat] mean and std.dev: (4081.99, 2834.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 334, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (336.80, 129.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1866, 3947, 6565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15963) [M::mem_pestat] mean and std.dev: (4320.54, 2813.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20662) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1649, 3699, 6402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15908) [M::mem_pestat] mean and std.dev: (4162.88, 2832.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20661) [M::mem_process_seqs] Processed 1333334 reads in 383.959 CPU sec, 24.541 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7705, 129426, 6622, 7244) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1675, 3696, 6357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15721) [M::mem_pestat] mean and std.dev: (4144.69, 2819.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 335, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (336.63, 129.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1820, 3919, 6575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16085) [M::mem_pestat] mean and std.dev: (4286.69, 2808.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20840) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1591, 3661, 6294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15700) [M::mem_pestat] mean and std.dev: (4109.20, 2823.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20403) [M::mem_process_seqs] Processed 1333334 reads in 392.068 CPU sec, 24.963 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7382, 129384, 6673, 7331) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1606, 3600, 6384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15940) [M::mem_pestat] mean and std.dev: (4107.99, 2840.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 332, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (334.56, 129.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1823, 3842, 6555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16019) [M::mem_pestat] mean and std.dev: (4262.77, 2807.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20751) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1640, 3673, 6452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16076) [M::mem_pestat] mean and std.dev: (4153.28, 2840.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20888) [M::mem_process_seqs] Processed 1333334 reads in 428.140 CPU sec, 27.234 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7425, 129133, 6794, 7257) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1647, 3666, 6355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15771) [M::mem_pestat] mean and std.dev: (4129.00, 2823.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 333, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (335.91, 129.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1863, 3943, 6532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15870) [M::mem_pestat] mean and std.dev: (4289.16, 2813.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20539) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1693, 3669, 6430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15904) [M::mem_pestat] mean and std.dev: (4150.56, 2792.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20641) [M::mem_process_seqs] Processed 1333334 reads in 405.953 CPU sec, 25.962 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7566, 128655, 6879, 7282) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1624, 3750, 6326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15730) [M::mem_pestat] mean and std.dev: (4126.86, 2818.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 335, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (337.49, 129.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1792, 3874, 6502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15922) [M::mem_pestat] mean and std.dev: (4237.22, 2802.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20632) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1705, 3692, 6416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15838) [M::mem_pestat] mean and std.dev: (4160.13, 2811.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20549) [M::mem_process_seqs] Processed 1333334 reads in 390.037 CPU sec, 24.853 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7555, 128061, 6909, 7465) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1619, 3657, 6274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15584) [M::mem_pestat] mean and std.dev: (4083.33, 2802.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20239) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 336, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (338.09, 129.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1930, 3868, 6682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16186) [M::mem_pestat] mean and std.dev: (4323.18, 2812.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20938) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1654, 3736, 6387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15853) [M::mem_pestat] mean and std.dev: (4135.90, 2792.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20586) [M::mem_process_seqs] Processed 1333334 reads in 385.506 CPU sec, 24.631 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7521, 128924, 6876, 7163) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1619, 3689, 6459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16139) [M::mem_pestat] mean and std.dev: (4152.00, 2851.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20979) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 335, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (337.72, 129.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1811, 3881, 6627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16259) [M::mem_pestat] mean and std.dev: (4291.99, 2812.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21075) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1673, 3693, 6333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15653) [M::mem_pestat] mean and std.dev: (4128.08, 2785.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20313) [M::mem_process_seqs] Processed 1333334 reads in 390.269 CPU sec, 24.830 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7469, 128862, 6782, 7398) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1700, 3745, 6471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16013) [M::mem_pestat] mean and std.dev: (4180.24, 2826.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 335, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (337.94, 130.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1832, 3908, 6565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16031) [M::mem_pestat] mean and std.dev: (4292.34, 2821.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20764) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1653, 3658, 6321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15657) [M::mem_pestat] mean and std.dev: (4103.35, 2791.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20325) [M::mem_process_seqs] Processed 1333334 reads in 395.747 CPU sec, 25.238 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7494, 127429, 6770, 7162) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1625, 3680, 6400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15950) [M::mem_pestat] mean and std.dev: (4137.64, 2839.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 335, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (337.94, 129.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1865, 3884, 6642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16196) [M::mem_pestat] mean and std.dev: (4309.11, 2828.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20973) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1713, 3684, 6334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15576) [M::mem_pestat] mean and std.dev: (4148.68, 2796.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20197) [M::mem_process_seqs] Processed 1333334 reads in 407.676 CPU sec, 25.938 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7411, 127373, 6895, 7337) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1673, 3737, 6487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16115) [M::mem_pestat] mean and std.dev: (4165.77, 2826.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20929) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 335, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (337.62, 129.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1889, 3896, 6545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15857) [M::mem_pestat] mean and std.dev: (4288.18, 2787.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20513) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1661, 3738, 6524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16250) [M::mem_pestat] mean and std.dev: (4198.27, 2841.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21113) [M::mem_process_seqs] Processed 1333334 reads in 422.107 CPU sec, 26.956 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7358, 127895, 6874, 7225) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1627, 3611, 6281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15589) [M::mem_pestat] mean and std.dev: (4108.08, 2805.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20243) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 336, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (338.65, 130.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1825, 3866, 6528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15934) [M::mem_pestat] mean and std.dev: (4252.51, 2799.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20637) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1613, 3765, 6361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15857) [M::mem_pestat] mean and std.dev: (4148.74, 2820.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20605) [M::mem_process_seqs] Processed 1333334 reads in 396.410 CPU sec, 25.233 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7475, 128647, 6765, 7305) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1562, 3572, 6323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15845) [M::mem_pestat] mean and std.dev: (4062.09, 2820.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 336, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (338.39, 130.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1911, 4005, 6691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16251) [M::mem_pestat] mean and std.dev: (4361.55, 2801.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21031) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1684, 3722, 6350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15682) [M::mem_pestat] mean and std.dev: (4143.80, 2785.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20348) [M::mem_process_seqs] Processed 1333334 reads in 398.748 CPU sec, 25.382 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7398, 127325, 6769, 7270) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1489, 3603, 6281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15865) [M::mem_pestat] mean and std.dev: (4052.48, 2835.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 336, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (339.23, 130.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1808, 3823, 6573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16103) [M::mem_pestat] mean and std.dev: (4252.66, 2806.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20868) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1642, 3730, 6466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16114) [M::mem_pestat] mean and std.dev: (4167.54, 2830.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20938) [M::mem_process_seqs] Processed 1333334 reads in 396.082 CPU sec, 25.210 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7270, 127067, 6717, 7329) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1630, 3603, 6309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15667) [M::mem_pestat] mean and std.dev: (4085.89, 2797.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20346) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 336, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (338.67, 130.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1825, 3928, 6560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16030) [M::mem_pestat] mean and std.dev: (4286.89, 2820.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20765) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1613, 3653, 6268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15578) [M::mem_pestat] mean and std.dev: (4097.48, 2791.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20233) [M::mem_process_seqs] Processed 1333334 reads in 412.125 CPU sec, 26.217 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7383, 126797, 6811, 7363) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1631, 3713, 6403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15947) [M::mem_pestat] mean and std.dev: (4151.77, 2822.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 337, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (338.50, 130.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1885, 3943, 6553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15889) [M::mem_pestat] mean and std.dev: (4281.45, 2784.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20557) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1665, 3759, 6381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15813) [M::mem_pestat] mean and std.dev: (4176.33, 2801.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20529) [M::mem_process_seqs] Processed 1333334 reads in 405.331 CPU sec, 25.839 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7568, 127419, 6719, 7377) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1575, 3548, 6271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15663) [M::mem_pestat] mean and std.dev: (4039.68, 2816.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 338, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (339.91, 130.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1907, 3991, 6624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16058) [M::mem_pestat] mean and std.dev: (4351.62, 2793.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20775) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1700, 3768, 6533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16199) [M::mem_pestat] mean and std.dev: (4221.54, 2828.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21032) [M::mem_process_seqs] Processed 1333334 reads in 404.114 CPU sec, 25.786 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7563, 127456, 6825, 7301) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1653, 3660, 6345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15729) [M::mem_pestat] mean and std.dev: (4116.74, 2813.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 337, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (339.58, 131.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1868, 3975, 6671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16277) [M::mem_pestat] mean and std.dev: (4345.83, 2813.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21080) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1702, 3700, 6370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15706) [M::mem_pestat] mean and std.dev: (4173.66, 2803.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20374) [M::mem_process_seqs] Processed 1333334 reads in 392.214 CPU sec, 25.082 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7595, 127321, 6897, 7268) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1701, 3766, 6353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15657) [M::mem_pestat] mean and std.dev: (4142.19, 2772.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 338, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (339.99, 131.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1861, 3904, 6512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15814) [M::mem_pestat] mean and std.dev: (4268.56, 2765.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20465) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1602, 3616, 6341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15819) [M::mem_pestat] mean and std.dev: (4090.47, 2807.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20558) [M::mem_process_seqs] Processed 1333334 reads in 393.072 CPU sec, 25.116 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7457, 127577, 6851, 7380) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1618, 3613, 6298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15658) [M::mem_pestat] mean and std.dev: (4092.68, 2797.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20338) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 338, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (340.31, 131.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1859, 3922, 6595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16067) [M::mem_pestat] mean and std.dev: (4314.52, 2820.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20803) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1664, 3736, 6388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15836) [M::mem_pestat] mean and std.dev: (4169.24, 2827.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20560) [M::mem_process_seqs] Processed 1333334 reads in 392.627 CPU sec, 25.020 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7490, 126890, 6928, 7308) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1691, 3801, 6444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15950) [M::mem_pestat] mean and std.dev: (4191.13, 2820.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 338, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (340.72, 131.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1854, 3956, 6533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15891) [M::mem_pestat] mean and std.dev: (4290.87, 2795.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20570) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1669, 3763, 6453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16021) [M::mem_pestat] mean and std.dev: (4199.34, 2831.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20805) [M::mem_process_seqs] Processed 1333334 reads in 397.933 CPU sec, 25.405 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7256, 127095, 6746, 7219) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1594, 3660, 6486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16270) [M::mem_pestat] mean and std.dev: (4150.96, 2882.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21162) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 335, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (337.43, 130.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1817, 3883, 6465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15761) [M::mem_pestat] mean and std.dev: (4248.29, 2767.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20409) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1608, 3676, 6430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16074) [M::mem_pestat] mean and std.dev: (4147.93, 2842.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20896) [M::mem_process_seqs] Processed 1333334 reads in 440.695 CPU sec, 28.136 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7587, 126895, 6774, 7366) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1657, 3696, 6355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15751) [M::mem_pestat] mean and std.dev: (4123.72, 2802.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 339, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (340.62, 132.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1791, 3789, 6533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16017) [M::mem_pestat] mean and std.dev: (4216.55, 2807.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20759) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1682, 3805, 6475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16061) [M::mem_pestat] mean and std.dev: (4208.17, 2821.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20854) [M::mem_process_seqs] Processed 1333334 reads in 402.512 CPU sec, 25.613 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7562, 127936, 6887, 7241) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1587, 3637, 6422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16092) [M::mem_pestat] mean and std.dev: (4105.91, 2846.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20927) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 338, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (341.02, 132.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1044) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1921, 3987, 6652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16114) [M::mem_pestat] mean and std.dev: (4336.99, 2792.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20845) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1627, 3639, 6367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15847) [M::mem_pestat] mean and std.dev: (4127.17, 2836.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20587) [M::mem_process_seqs] Processed 1333334 reads in 390.460 CPU sec, 24.815 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7383, 126348, 6706, 7416) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1688, 3715, 6377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15755) [M::mem_pestat] mean and std.dev: (4150.58, 2814.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 339, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (340.79, 131.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1869, 3916, 6512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15798) [M::mem_pestat] mean and std.dev: (4301.27, 2799.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20441) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1626, 3691, 6430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16038) [M::mem_pestat] mean and std.dev: (4163.72, 2826.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20842) [M::mem_process_seqs] Processed 1333334 reads in 394.345 CPU sec, 25.080 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7393, 127118, 6861, 7392) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1626, 3694, 6415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15993) [M::mem_pestat] mean and std.dev: (4142.87, 2845.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 338, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (340.40, 130.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1861, 3898, 6587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16039) [M::mem_pestat] mean and std.dev: (4291.06, 2810.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20765) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1670, 3650, 6345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15695) [M::mem_pestat] mean and std.dev: (4146.88, 2808.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20370) [M::mem_process_seqs] Processed 1333334 reads in 407.438 CPU sec, 25.941 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7412, 126992, 6816, 7411) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1685, 3703, 6431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15923) [M::mem_pestat] mean and std.dev: (4158.83, 2815.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 339, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (340.71, 131.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1820, 3885, 6587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16121) [M::mem_pestat] mean and std.dev: (4285.27, 2794.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20888) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1637, 3686, 6454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16088) [M::mem_pestat] mean and std.dev: (4167.51, 2848.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20905) [M::mem_process_seqs] Processed 1333334 reads in 402.608 CPU sec, 25.620 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7480, 126520, 6601, 7321) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1562, 3670, 6295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15761) [M::mem_pestat] mean and std.dev: (4096.75, 2835.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 338, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (341.00, 131.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1842, 3851, 6531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15909) [M::mem_pestat] mean and std.dev: (4258.99, 2813.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20598) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1683, 3700, 6361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15717) [M::mem_pestat] mean and std.dev: (4156.38, 2808.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20395) [M::mem_process_seqs] Processed 1333334 reads in 402.623 CPU sec, 25.695 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7401, 126382, 6671, 7251) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1647, 3648, 6321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15669) [M::mem_pestat] mean and std.dev: (4117.16, 2812.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 339, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (340.92, 132.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1845, 3882, 6496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15798) [M::mem_pestat] mean and std.dev: (4259.63, 2780.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20449) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1637, 3741, 6368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15830) [M::mem_pestat] mean and std.dev: (4155.59, 2838.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20561) [M::mem_process_seqs] Processed 1333334 reads in 400.329 CPU sec, 25.720 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7609, 126256, 6773, 7427) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1610, 3680, 6402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15986) [M::mem_pestat] mean and std.dev: (4140.75, 2852.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 336, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (338.31, 130.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1926, 3983, 6640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16068) [M::mem_pestat] mean and std.dev: (4353.12, 2810.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20782) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1632, 3547, 6340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15756) [M::mem_pestat] mean and std.dev: (4106.13, 2819.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20464) [M::mem_process_seqs] Processed 1333334 reads in 423.374 CPU sec, 26.946 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7729, 120679, 6821, 7389) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1567, 3601, 6287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15727) [M::mem_pestat] mean and std.dev: (4054.74, 2827.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 329, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (330.26, 125.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1802, 3904, 6619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16253) [M::mem_pestat] mean and std.dev: (4283.85, 2815.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21070) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1561, 3648, 6421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16141) [M::mem_pestat] mean and std.dev: (4114.58, 2829.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21001) [M::mem_process_seqs] Processed 1333334 reads in 521.022 CPU sec, 33.098 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7817, 118995, 6872, 7438) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1586, 3671, 6335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15833) [M::mem_pestat] mean and std.dev: (4114.72, 2855.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (332.48, 126.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1850, 3905, 6556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15968) [M::mem_pestat] mean and std.dev: (4279.58, 2802.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20674) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1644, 3569, 6334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15714) [M::mem_pestat] mean and std.dev: (4096.36, 2806.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20404) [M::mem_process_seqs] Processed 1333334 reads in 516.374 CPU sec, 32.828 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7748, 116073, 7211, 7718) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1612, 3605, 6368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15880) [M::mem_pestat] mean and std.dev: (4103.54, 2830.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 334, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (336.01, 128.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1822, 3873, 6549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16003) [M::mem_pestat] mean and std.dev: (4265.28, 2814.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20730) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1618, 3683, 6428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16048) [M::mem_pestat] mean and std.dev: (4139.29, 2827.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20858) [M::mem_process_seqs] Processed 1333334 reads in 509.623 CPU sec, 32.408 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7786, 112040, 7165, 7662) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1527, 3539, 6285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15801) [M::mem_pestat] mean and std.dev: (4034.24, 2840.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 333, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (333.56, 126.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1805, 3857, 6512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15926) [M::mem_pestat] mean and std.dev: (4249.18, 2803.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20633) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1639, 3754, 6527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16303) [M::mem_pestat] mean and std.dev: (4201.53, 2866.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21191) [M::mem_process_seqs] Processed 1333334 reads in 567.285 CPU sec, 36.427 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7853, 114385, 7061, 7759) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1570, 3619, 6308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15784) [M::mem_pestat] mean and std.dev: (4071.41, 2812.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 335, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (336.35, 128.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1838, 3880, 6580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16064) [M::mem_pestat] mean and std.dev: (4289.94, 2807.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20806) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1685, 3759, 6377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15761) [M::mem_pestat] mean and std.dev: (4174.91, 2814.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20453) [M::mem_process_seqs] Processed 1333334 reads in 529.843 CPU sec, 33.639 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7949, 112461, 7003, 7611) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1585, 3502, 6200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15430) [M::mem_pestat] mean and std.dev: (4012.07, 2801.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20045) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 335, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (335.63, 127.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1870, 3969, 6553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15919) [M::mem_pestat] mean and std.dev: (4309.38, 2786.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20602) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1653, 3623, 6281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15537) [M::mem_pestat] mean and std.dev: (4117.93, 2811.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20165) [M::mem_process_seqs] Processed 1333334 reads in 563.931 CPU sec, 36.078 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7730, 111644, 7062, 7674) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1618, 3616, 6331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15757) [M::mem_pestat] mean and std.dev: (4093.15, 2821.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 335, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (335.25, 127.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1867, 4009, 6636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16174) [M::mem_pestat] mean and std.dev: (4344.53, 2809.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20943) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1638, 3631, 6385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15879) [M::mem_pestat] mean and std.dev: (4134.13, 2832.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20626) [M::mem_process_seqs] Processed 1333334 reads in 556.951 CPU sec, 36.174 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7868, 113263, 7206, 7566) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1601, 3630, 6354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15860) [M::mem_pestat] mean and std.dev: (4086.59, 2814.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 335, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (335.81, 127.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1941, 3949, 6689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16185) [M::mem_pestat] mean and std.dev: (4361.19, 2810.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20933) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1667, 3743, 6385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15821) [M::mem_pestat] mean and std.dev: (4172.14, 2822.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20539) [M::mem_process_seqs] Processed 1333334 reads in 549.450 CPU sec, 35.245 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7493, 127025, 6707, 7385) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1640, 3641, 6370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15830) [M::mem_pestat] mean and std.dev: (4117.07, 2834.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (332.98, 126.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1913, 3898, 6492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15650) [M::mem_pestat] mean and std.dev: (4294.73, 2775.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20229) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1674, 3683, 6470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16062) [M::mem_pestat] mean and std.dev: (4168.85, 2826.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20858) [M::mem_process_seqs] Processed 1333334 reads in 464.947 CPU sec, 29.572 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7637, 126195, 6929, 7307) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1596, 3654, 6338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15822) [M::mem_pestat] mean and std.dev: (4095.15, 2836.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (333.50, 127.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1950, 3951, 6551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15753) [M::mem_pestat] mean and std.dev: (4315.82, 2772.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20354) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1653, 3701, 6421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15957) [M::mem_pestat] mean and std.dev: (4143.33, 2806.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20725) [M::mem_process_seqs] Processed 1333334 reads in 455.804 CPU sec, 29.024 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7463, 126361, 6673, 7323) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1578, 3592, 6247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15585) [M::mem_pestat] mean and std.dev: (4073.43, 2826.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20254) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 329, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (331.78, 126.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1787, 3826, 6473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15845) [M::mem_pestat] mean and std.dev: (4223.87, 2802.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20531) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1629, 3714, 6428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16026) [M::mem_pestat] mean and std.dev: (4141.41, 2827.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20825) [M::mem_process_seqs] Processed 1333334 reads in 480.704 CPU sec, 30.548 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7671, 130240, 6814, 7429) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1639, 3637, 6407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15943) [M::mem_pestat] mean and std.dev: (4131.45, 2829.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (333.68, 126.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1853, 3955, 6587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16055) [M::mem_pestat] mean and std.dev: (4291.61, 2800.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20789) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1724, 3734, 6319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15509) [M::mem_pestat] mean and std.dev: (4148.54, 2782.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20104) [M::mem_process_seqs] Processed 1333334 reads in 419.606 CPU sec, 26.718 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7414, 130250, 6881, 7276) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1637, 3603, 6336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15734) [M::mem_pestat] mean and std.dev: (4101.82, 2833.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 331, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (333.83, 127.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1848, 3902, 6559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15981) [M::mem_pestat] mean and std.dev: (4286.89, 2810.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20692) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1606, 3597, 6243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15517) [M::mem_pestat] mean and std.dev: (4072.16, 2796.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20154) [M::mem_process_seqs] Processed 1333334 reads in 398.652 CPU sec, 25.456 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7546, 130242, 6731, 7199) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1519, 3656, 6293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15841) [M::mem_pestat] mean and std.dev: (4063.35, 2814.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (333.43, 127.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1804, 3815, 6436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15700) [M::mem_pestat] mean and std.dev: (4210.44, 2793.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20332) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1747, 3807, 6472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15922) [M::mem_pestat] mean and std.dev: (4218.75, 2815.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20647) [M::mem_process_seqs] Processed 1333334 reads in 421.001 CPU sec, 26.847 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7625, 131248, 6823, 7326) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1652, 3745, 6431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15989) [M::mem_pestat] mean and std.dev: (4178.55, 2838.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (333.41, 127.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1831, 3876, 6476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15766) [M::mem_pestat] mean and std.dev: (4258.99, 2803.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20411) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1613, 3643, 6345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15809) [M::mem_pestat] mean and std.dev: (4118.21, 2816.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20541) [M::mem_process_seqs] Processed 1333334 reads in 417.625 CPU sec, 26.579 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7673, 128303, 6904, 7362) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1566, 3670, 6315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15813) [M::mem_pestat] mean and std.dev: (4090.75, 2827.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (333.89, 127.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1853, 3901, 6489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15761) [M::mem_pestat] mean and std.dev: (4266.52, 2791.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20397) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1616, 3718, 6431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16061) [M::mem_pestat] mean and std.dev: (4156.89, 2831.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20876) [M::mem_process_seqs] Processed 1333334 reads in 486.883 CPU sec, 30.939 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7453, 123585, 6879, 7407) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1582, 3581, 6336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15844) [M::mem_pestat] mean and std.dev: (4069.42, 2818.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 329, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (330.46, 125.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1883, 3955, 6625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16109) [M::mem_pestat] mean and std.dev: (4311.20, 2813.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20851) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1656, 3731, 6361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15771) [M::mem_pestat] mean and std.dev: (4148.32, 2801.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20476) [M::mem_process_seqs] Processed 1333334 reads in 532.132 CPU sec, 33.935 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7553, 129910, 6833, 7174) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1637, 3608, 6216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15374) [M::mem_pestat] mean and std.dev: (4047.52, 2783.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 333, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (334.99, 127.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1861, 3925, 6646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16216) [M::mem_pestat] mean and std.dev: (4306.97, 2820.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21001) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1595, 3593, 6378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15944) [M::mem_pestat] mean and std.dev: (4096.98, 2828.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20727) [M::mem_process_seqs] Processed 1333334 reads in 435.506 CPU sec, 27.766 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7386, 129975, 6910, 7215) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1611, 3620, 6277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15609) [M::mem_pestat] mean and std.dev: (4087.01, 2806.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20275) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 332, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (335.10, 127.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1821, 3927, 6623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16227) [M::mem_pestat] mean and std.dev: (4291.52, 2825.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21029) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1667, 3690, 6358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15740) [M::mem_pestat] mean and std.dev: (4154.16, 2805.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20431) [M::mem_process_seqs] Processed 1333334 reads in 446.099 CPU sec, 28.363 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7430, 129293, 6841, 7327) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1682, 3765, 6450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15986) [M::mem_pestat] mean and std.dev: (4190.72, 2840.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 333, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (336.20, 128.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1823, 3909, 6589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16121) [M::mem_pestat] mean and std.dev: (4296.16, 2809.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20887) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1662, 3680, 6323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15645) [M::mem_pestat] mean and std.dev: (4142.32, 2818.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20306) [M::mem_process_seqs] Processed 1333334 reads in 413.913 CPU sec, 26.370 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7625, 127677, 6774, 7444) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1542, 3583, 6402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16122) [M::mem_pestat] mean and std.dev: (4092.83, 2865.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 333, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (336.19, 128.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1808, 3829, 6448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15728) [M::mem_pestat] mean and std.dev: (4213.21, 2790.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20368) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1610, 3651, 6459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16157) [M::mem_pestat] mean and std.dev: (4141.48, 2844.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21006) [M::mem_process_seqs] Processed 1333334 reads in 470.576 CPU sec, 29.906 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7382, 128867, 6841, 7240) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1599, 3623, 6336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15810) [M::mem_pestat] mean and std.dev: (4110.69, 2823.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 335, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (336.98, 128.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1867, 3909, 6525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15841) [M::mem_pestat] mean and std.dev: (4276.52, 2793.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20499) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1670, 3684, 6380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15800) [M::mem_pestat] mean and std.dev: (4148.85, 2817.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20510) [M::mem_process_seqs] Processed 1333334 reads in 427.893 CPU sec, 27.186 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7496, 129454, 6916, 7318) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1650, 3625, 6229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15387) [M::mem_pestat] mean and std.dev: (4068.51, 2784.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19966) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 335, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (336.80, 128.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1826, 3832, 6460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15728) [M::mem_pestat] mean and std.dev: (4223.03, 2759.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20362) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1687, 3700, 6425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15901) [M::mem_pestat] mean and std.dev: (4166.55, 2823.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20639) [M::mem_process_seqs] Processed 1333334 reads in 426.313 CPU sec, 27.077 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7590, 128305, 6898, 7352) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1679, 3702, 6368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15746) [M::mem_pestat] mean and std.dev: (4137.50, 2809.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 334, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (336.04, 128.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1873, 3924, 6632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16150) [M::mem_pestat] mean and std.dev: (4314.14, 2806.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20909) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1615, 3565, 6290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15640) [M::mem_pestat] mean and std.dev: (4073.39, 2821.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20315) [M::mem_process_seqs] Processed 1333334 reads in 419.444 CPU sec, 26.693 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7574, 128796, 6796, 7412) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1677, 3760, 6436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15954) [M::mem_pestat] mean and std.dev: (4156.13, 2825.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 334, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (336.23, 128.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1841, 3922, 6578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16052) [M::mem_pestat] mean and std.dev: (4294.32, 2809.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20789) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1610, 3584, 6354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15842) [M::mem_pestat] mean and std.dev: (4093.89, 2811.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20586) [M::mem_process_seqs] Processed 1333334 reads in 429.906 CPU sec, 27.359 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7458, 127257, 6753, 7296) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1562, 3648, 6357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15947) [M::mem_pestat] mean and std.dev: (4092.11, 2829.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 332, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (334.82, 127.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1874, 3907, 6576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15980) [M::mem_pestat] mean and std.dev: (4277.75, 2785.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20682) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1651, 3709, 6370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15808) [M::mem_pestat] mean and std.dev: (4136.31, 2806.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20527) [M::mem_process_seqs] Processed 1333334 reads in 494.065 CPU sec, 31.510 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7508, 129576, 6875, 7297) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1622, 3630, 6392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15932) [M::mem_pestat] mean and std.dev: (4119.92, 2825.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 335, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (337.44, 128.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1833, 3863, 6530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15924) [M::mem_pestat] mean and std.dev: (4258.76, 2804.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20621) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1713, 3811, 6460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15954) [M::mem_pestat] mean and std.dev: (4207.10, 2823.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20701) [M::mem_process_seqs] Processed 1333334 reads in 437.390 CPU sec, 27.793 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7430, 128243, 6943, 7341) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1620, 3626, 6417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16011) [M::mem_pestat] mean and std.dev: (4123.14, 2843.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 336, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (338.35, 129.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1819, 3923, 6484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15814) [M::mem_pestat] mean and std.dev: (4274.81, 2803.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20479) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1624, 3628, 6265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15547) [M::mem_pestat] mean and std.dev: (4088.14, 2792.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20188) [M::mem_process_seqs] Processed 1333334 reads in 411.039 CPU sec, 26.122 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7633, 127238, 6969, 7356) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1630, 3677, 6416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15988) [M::mem_pestat] mean and std.dev: (4138.38, 2831.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 336, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (338.68, 128.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1797, 3856, 6584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16158) [M::mem_pestat] mean and std.dev: (4268.65, 2828.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20945) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1651, 3729, 6408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15922) [M::mem_pestat] mean and std.dev: (4148.64, 2807.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20679) [M::mem_process_seqs] Processed 1333334 reads in 445.092 CPU sec, 28.558 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7409, 127956, 6751, 7282) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1660, 3744, 6460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16060) [M::mem_pestat] mean and std.dev: (4169.24, 2822.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 336, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (337.96, 129.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1904, 3932, 6640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16112) [M::mem_pestat] mean and std.dev: (4321.84, 2795.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20848) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1610, 3635, 6400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15980) [M::mem_pestat] mean and std.dev: (4151.61, 2850.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20770) [M::mem_process_seqs] Processed 1333334 reads in 431.292 CPU sec, 27.488 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7556, 126982, 6916, 7200) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1656, 3642, 6373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15807) [M::mem_pestat] mean and std.dev: (4127.48, 2831.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 336, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (338.08, 129.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1886, 3861, 6595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16013) [M::mem_pestat] mean and std.dev: (4271.76, 2796.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20722) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1730, 3841, 6421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15803) [M::mem_pestat] mean and std.dev: (4210.67, 2800.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20494) [M::mem_process_seqs] Processed 1333334 reads in 432.031 CPU sec, 27.426 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7593, 124918, 6677, 7220) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1548, 3587, 6384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16056) [M::mem_pestat] mean and std.dev: (4094.76, 2853.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 333, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (335.89, 128.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1822, 3926, 6509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15883) [M::mem_pestat] mean and std.dev: (4287.67, 2817.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20570) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1717, 3706, 6448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15910) [M::mem_pestat] mean and std.dev: (4171.47, 2825.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20641) [M::mem_process_seqs] Processed 1333334 reads in 471.353 CPU sec, 29.970 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7423, 127799, 6821, 7271) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1657, 3630, 6423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15955) [M::mem_pestat] mean and std.dev: (4121.91, 2826.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 335, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (337.41, 128.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1838, 3992, 6548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15968) [M::mem_pestat] mean and std.dev: (4302.86, 2807.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20678) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1635, 3590, 6354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15792) [M::mem_pestat] mean and std.dev: (4113.75, 2805.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20511) [M::mem_process_seqs] Processed 1333334 reads in 408.127 CPU sec, 26.025 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7580, 127403, 6840, 7503) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1656, 3662, 6373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15807) [M::mem_pestat] mean and std.dev: (4121.23, 2815.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 336, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (338.24, 129.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1835, 3942, 6583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16079) [M::mem_pestat] mean and std.dev: (4298.02, 2829.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20827) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1656, 3712, 6432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15984) [M::mem_pestat] mean and std.dev: (4177.88, 2825.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20760) [M::mem_process_seqs] Processed 1333334 reads in 426.967 CPU sec, 28.587 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7552, 127651, 6724, 7254) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1676, 3760, 6436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15956) [M::mem_pestat] mean and std.dev: (4173.07, 2831.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 336, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (338.30, 129.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1751, 3776, 6378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15632) [M::mem_pestat] mean and std.dev: (4187.30, 2781.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20259) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1697, 3795, 6465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16001) [M::mem_pestat] mean and std.dev: (4211.22, 2823.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20769) [M::mem_process_seqs] Processed 1333334 reads in 410.224 CPU sec, 26.079 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7410, 127702, 6838, 7274) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1591, 3660, 6347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15859) [M::mem_pestat] mean and std.dev: (4112.91, 2836.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 336, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (337.91, 129.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1905, 3969, 6588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15954) [M::mem_pestat] mean and std.dev: (4310.46, 2793.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20637) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1718, 3749, 6372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15680) [M::mem_pestat] mean and std.dev: (4161.03, 2808.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20334) [M::mem_process_seqs] Processed 1333334 reads in 410.679 CPU sec, 26.104 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7561, 127075, 6693, 7273) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1675, 3693, 6379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15787) [M::mem_pestat] mean and std.dev: (4143.15, 2827.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 336, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (338.95, 129.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1961, 4042, 6627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15959) [M::mem_pestat] mean and std.dev: (4362.68, 2788.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20625) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1709, 3728, 6434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15884) [M::mem_pestat] mean and std.dev: (4163.93, 2795.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20609) [M::mem_process_seqs] Processed 1333334 reads in 420.643 CPU sec, 26.758 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7427, 126900, 6794, 7331) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1664, 3669, 6407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15893) [M::mem_pestat] mean and std.dev: (4157.67, 2827.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 336, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (338.36, 129.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1906, 3982, 6505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15703) [M::mem_pestat] mean and std.dev: (4308.57, 2774.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20302) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1653, 3676, 6406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15912) [M::mem_pestat] mean and std.dev: (4136.75, 2809.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20665) [M::mem_process_seqs] Processed 1333334 reads in 410.935 CPU sec, 26.258 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7448, 127183, 6767, 7344) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1608, 3619, 6405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15999) [M::mem_pestat] mean and std.dev: (4110.76, 2832.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 337, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (338.95, 129.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1844, 3874, 6511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15845) [M::mem_pestat] mean and std.dev: (4265.66, 2793.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1611, 3590, 6345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15813) [M::mem_pestat] mean and std.dev: (4101.59, 2817.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20547) [M::mem_process_seqs] Processed 1333334 reads in 429.451 CPU sec, 27.381 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7531, 128996, 6637, 7297) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1570, 3592, 6298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15754) [M::mem_pestat] mean and std.dev: (4083.85, 2821.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 336, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (338.62, 130.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1787, 3829, 6592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16202) [M::mem_pestat] mean and std.dev: (4254.35, 2815.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21007) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1650, 3765, 6560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16380) [M::mem_pestat] mean and std.dev: (4216.19, 2860.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21290) [M::mem_process_seqs] Processed 1333334 reads in 427.208 CPU sec, 27.248 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7424, 127393, 6843, 7188) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1545, 3547, 6332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15906) [M::mem_pestat] mean and std.dev: (4069.42, 2839.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 338, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (339.84, 129.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1889, 3952, 6574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15944) [M::mem_pestat] mean and std.dev: (4318.33, 2790.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20629) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1609, 3723, 6339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15799) [M::mem_pestat] mean and std.dev: (4134.70, 2820.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20529) [M::mem_process_seqs] Processed 1333334 reads in 454.843 CPU sec, 28.852 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7578, 127600, 6907, 7223) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1692, 3744, 6436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15924) [M::mem_pestat] mean and std.dev: (4177.20, 2807.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 338, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (340.18, 130.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1843, 3926, 6686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16372) [M::mem_pestat] mean and std.dev: (4308.98, 2822.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21215) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1657, 3825, 6463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16075) [M::mem_pestat] mean and std.dev: (4197.12, 2826.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20881) [M::mem_process_seqs] Processed 1333334 reads in 401.561 CPU sec, 25.489 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7453, 127419, 6791, 7326) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1672, 3725, 6410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15886) [M::mem_pestat] mean and std.dev: (4154.74, 2833.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 337, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (339.49, 129.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1853, 3954, 6549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15941) [M::mem_pestat] mean and std.dev: (4292.06, 2797.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20637) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1602, 3576, 6286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15654) [M::mem_pestat] mean and std.dev: (4082.33, 2817.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20338) [M::mem_process_seqs] Processed 1333334 reads in 438.510 CPU sec, 27.840 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7451, 127033, 6816, 7356) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1596, 3623, 6328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15792) [M::mem_pestat] mean and std.dev: (4096.53, 2823.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 339, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (340.93, 131.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1861, 3936, 6621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16141) [M::mem_pestat] mean and std.dev: (4317.36, 2824.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20901) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1658, 3598, 6431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15977) [M::mem_pestat] mean and std.dev: (4151.56, 2829.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20750) [M::mem_process_seqs] Processed 1333334 reads in 415.164 CPU sec, 26.479 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7397, 127570, 6936, 7203) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1629, 3692, 6463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16131) [M::mem_pestat] mean and std.dev: (4157.78, 2832.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 340, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (341.89, 131.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1883, 3877, 6538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15848) [M::mem_pestat] mean and std.dev: (4281.55, 2814.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20503) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1635, 3678, 6369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15837) [M::mem_pestat] mean and std.dev: (4128.57, 2811.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20571) [M::mem_process_seqs] Processed 1333334 reads in 404.378 CPU sec, 25.825 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7309, 127471, 6727, 7311) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1579, 3634, 6423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16111) [M::mem_pestat] mean and std.dev: (4110.42, 2826.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 339, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (340.81, 131.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1866, 4018, 6674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16290) [M::mem_pestat] mean and std.dev: (4336.82, 2812.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21098) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1667, 3693, 6403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15875) [M::mem_pestat] mean and std.dev: (4160.04, 2812.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20611) [M::mem_process_seqs] Processed 1333334 reads in 415.967 CPU sec, 26.349 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7507, 127629, 6847, 7247) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1588, 3585, 6386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15982) [M::mem_pestat] mean and std.dev: (4086.61, 2840.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 337, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (339.65, 130.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1808, 3884, 6485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15839) [M::mem_pestat] mean and std.dev: (4261.32, 2801.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20516) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1652, 3700, 6416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15944) [M::mem_pestat] mean and std.dev: (4152.46, 2812.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20708) [M::mem_process_seqs] Processed 1333334 reads in 434.715 CPU sec, 27.692 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7418, 127609, 6935, 7310) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1575, 3649, 6316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15798) [M::mem_pestat] mean and std.dev: (4095.00, 2805.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 338, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (340.64, 131.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1852, 3925, 6675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16321) [M::mem_pestat] mean and std.dev: (4317.50, 2826.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21144) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1636, 3617, 6285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15583) [M::mem_pestat] mean and std.dev: (4085.70, 2784.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20232) [M::mem_process_seqs] Processed 1333334 reads in 401.357 CPU sec, 25.581 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7421, 128015, 6932, 7375) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1606, 3662, 6333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15787) [M::mem_pestat] mean and std.dev: (4104.31, 2816.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 337, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (340.10, 131.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1849, 3935, 6592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16078) [M::mem_pestat] mean and std.dev: (4297.14, 2826.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20821) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1638, 3682, 6390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15894) [M::mem_pestat] mean and std.dev: (4133.00, 2821.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20646) [M::mem_process_seqs] Processed 1333334 reads in 427.230 CPU sec, 27.135 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7383, 128216, 6795, 7320) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1695, 3691, 6477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16041) [M::mem_pestat] mean and std.dev: (4168.40, 2833.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 339, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (340.77, 131.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1816, 3895, 6511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15901) [M::mem_pestat] mean and std.dev: (4267.90, 2804.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20596) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1696, 3683, 6346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15646) [M::mem_pestat] mean and std.dev: (4140.85, 2786.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20296) [M::mem_process_seqs] Processed 1333334 reads in 418.997 CPU sec, 26.645 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7485, 127964, 6808, 7435) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1559, 3578, 6347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15923) [M::mem_pestat] mean and std.dev: (4080.50, 2843.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 333, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (335.68, 129.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1021) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1881, 3897, 6621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16101) [M::mem_pestat] mean and std.dev: (4298.11, 2810.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20841) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1634, 3606, 6378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15866) [M::mem_pestat] mean and std.dev: (4128.02, 2810.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20610) [M::mem_process_seqs] Processed 1333334 reads in 411.508 CPU sec, 26.314 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7421, 129955, 6718, 7079) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1637, 3685, 6389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15893) [M::mem_pestat] mean and std.dev: (4142.11, 2829.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 327, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (329.06, 125.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1862, 3878, 6603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16085) [M::mem_pestat] mean and std.dev: (4285.64, 2804.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20826) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1637, 3591, 6322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15692) [M::mem_pestat] mean and std.dev: (4092.67, 2789.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20377) [M::mem_process_seqs] Processed 1333334 reads in 473.950 CPU sec, 30.159 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7586, 125976, 6794, 7447) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1527, 3507, 6291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15819) [M::mem_pestat] mean and std.dev: (4037.37, 2825.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 328, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (330.49, 125.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1856, 3942, 6663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16277) [M::mem_pestat] mean and std.dev: (4328.29, 2823.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21084) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1659, 3640, 6374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15804) [M::mem_pestat] mean and std.dev: (4128.26, 2805.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20519) [M::mem_process_seqs] Processed 1333334 reads in 483.448 CPU sec, 30.767 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7688, 126077, 6976, 7148) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1568, 3635, 6411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16097) [M::mem_pestat] mean and std.dev: (4107.38, 2854.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 332, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (333.30, 127.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1854, 3872, 6557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15963) [M::mem_pestat] mean and std.dev: (4266.55, 2801.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20666) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1626, 3637, 6241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15471) [M::mem_pestat] mean and std.dev: (4079.62, 2783.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20086) [M::mem_process_seqs] Processed 1333334 reads in 449.203 CPU sec, 28.598 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7536, 124805, 7015, 7537) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1534, 3638, 6381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16075) [M::mem_pestat] mean and std.dev: (4093.99, 2841.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 330, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (332.41, 126.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1880, 3929, 6577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15971) [M::mem_pestat] mean and std.dev: (4286.24, 2799.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20668) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1667, 3675, 6252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15422) [M::mem_pestat] mean and std.dev: (4111.93, 2762.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20007) [M::mem_process_seqs] Processed 1333334 reads in 473.458 CPU sec, 30.160 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7733, 125241, 6792, 7592) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1592, 3694, 6380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15956) [M::mem_pestat] mean and std.dev: (4123.76, 2824.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 331, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (333.64, 127.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1757, 3809, 6462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15872) [M::mem_pestat] mean and std.dev: (4213.12, 2811.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20577) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1667, 3642, 6269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15473) [M::mem_pestat] mean and std.dev: (4097.42, 2774.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20075) [M::mem_process_seqs] Processed 1333334 reads in 448.793 CPU sec, 28.492 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7670, 123821, 6908, 7483) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1616, 3623, 6363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15857) [M::mem_pestat] mean and std.dev: (4110.06, 2818.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 330, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (331.76, 126.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1841, 3973, 6698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16412) [M::mem_pestat] mean and std.dev: (4333.43, 2826.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21269) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1650, 3678, 6401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15903) [M::mem_pestat] mean and std.dev: (4133.98, 2833.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20654) [M::mem_process_seqs] Processed 1333334 reads in 485.574 CPU sec, 30.806 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7606, 127834, 6793, 7438) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1594, 3704, 6355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15877) [M::mem_pestat] mean and std.dev: (4103.58, 2816.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 331, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (333.50, 127.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1829, 3967, 6576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16070) [M::mem_pestat] mean and std.dev: (4302.78, 2816.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20817) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1707, 3818, 6481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16029) [M::mem_pestat] mean and std.dev: (4211.75, 2818.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20803) [M::mem_process_seqs] Processed 1333334 reads in 433.951 CPU sec, 27.596 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7470, 128723, 6942, 7405) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1674, 3623, 6373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15771) [M::mem_pestat] mean and std.dev: (4106.42, 2816.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 330, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (332.51, 126.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1808, 3790, 6435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15689) [M::mem_pestat] mean and std.dev: (4220.44, 2791.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20316) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1686, 3733, 6434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15930) [M::mem_pestat] mean and std.dev: (4180.18, 2812.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20678) [M::mem_process_seqs] Processed 1333334 reads in 426.224 CPU sec, 27.406 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7641, 129659, 6774, 7361) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1653, 3709, 6445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16029) [M::mem_pestat] mean and std.dev: (4158.03, 2842.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 329, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (331.46, 125.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1889, 3947, 6528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15806) [M::mem_pestat] mean and std.dev: (4300.05, 2786.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20445) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1656, 3620, 6355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15753) [M::mem_pestat] mean and std.dev: (4116.68, 2807.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20452) [M::mem_process_seqs] Processed 1333334 reads in 457.561 CPU sec, 29.125 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7443, 129153, 6864, 7253) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1573, 3702, 6377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15985) [M::mem_pestat] mean and std.dev: (4118.85, 2846.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (243, 329, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (331.44, 126.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1865, 3967, 6568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15974) [M::mem_pestat] mean and std.dev: (4315.57, 2806.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20677) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1658, 3749, 6552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16340) [M::mem_pestat] mean and std.dev: (4199.43, 2844.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21234) [M::mem_process_seqs] Processed 1333334 reads in 458.059 CPU sec, 29.062 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7423, 122803, 6880, 7195) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1550, 3677, 6491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16373) [M::mem_pestat] mean and std.dev: (4144.28, 2866.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (242, 327, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (329.37, 125.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1837, 3868, 6535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15931) [M::mem_pestat] mean and std.dev: (4272.61, 2807.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20629) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1666, 3707, 6335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15673) [M::mem_pestat] mean and std.dev: (4122.67, 2793.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20342) [M::mem_process_seqs] Processed 1333334 reads in 526.004 CPU sec, 33.532 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7525, 130190, 6714, 7397) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1598, 3689, 6316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15752) [M::mem_pestat] mean and std.dev: (4110.14, 2816.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (333.72, 127.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1872, 3892, 6588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16020) [M::mem_pestat] mean and std.dev: (4298.05, 2818.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20736) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1699, 3815, 6548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16246) [M::mem_pestat] mean and std.dev: (4235.45, 2843.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21095) [M::mem_process_seqs] Processed 1333334 reads in 413.263 CPU sec, 26.244 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7450, 130628, 6782, 7321) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1617, 3654, 6349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15813) [M::mem_pestat] mean and std.dev: (4100.63, 2817.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (334.04, 127.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1913, 3947, 6590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15944) [M::mem_pestat] mean and std.dev: (4320.94, 2815.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20621) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1639, 3643, 6369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15829) [M::mem_pestat] mean and std.dev: (4139.31, 2820.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20559) [M::mem_process_seqs] Processed 1333334 reads in 421.399 CPU sec, 26.780 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7569, 130761, 6740, 7346) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1651, 3734, 6436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16006) [M::mem_pestat] mean and std.dev: (4164.29, 2834.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (333.65, 127.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1883, 3974, 6636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16142) [M::mem_pestat] mean and std.dev: (4325.21, 2806.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20895) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1728, 3706, 6432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15840) [M::mem_pestat] mean and std.dev: (4177.45, 2809.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20544) [M::mem_process_seqs] Processed 1333334 reads in 447.606 CPU sec, 28.427 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7391, 130255, 6869, 7399) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1636, 3616, 6369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15835) [M::mem_pestat] mean and std.dev: (4106.51, 2827.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (333.66, 127.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1824, 3853, 6492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15828) [M::mem_pestat] mean and std.dev: (4254.00, 2801.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20496) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1628, 3642, 6390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15914) [M::mem_pestat] mean and std.dev: (4139.75, 2825.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20676) [M::mem_process_seqs] Processed 1333334 reads in 412.810 CPU sec, 26.262 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7562, 127923, 6858, 7388) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1614, 3615, 6306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15690) [M::mem_pestat] mean and std.dev: (4088.26, 2813.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (245, 331, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (333.40, 126.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1821, 3928, 6659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16335) [M::mem_pestat] mean and std.dev: (4301.73, 2826.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21173) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1716, 3789, 6444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15900) [M::mem_pestat] mean and std.dev: (4203.68, 2799.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20628) [M::mem_process_seqs] Processed 1333334 reads in 443.076 CPU sec, 28.127 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7494, 125943, 6665, 7361) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1591, 3700, 6438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16132) [M::mem_pestat] mean and std.dev: (4126.43, 2852.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20979) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (244, 328, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (330.53, 125.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1908, 3990, 6574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15906) [M::mem_pestat] mean and std.dev: (4333.21, 2799.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20572) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1713, 3700, 6355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15639) [M::mem_pestat] mean and std.dev: (4151.63, 2782.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20281) [M::mem_process_seqs] Processed 1333334 reads in 526.394 CPU sec, 33.386 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7460, 129252, 6799, 7322) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1634, 3692, 6383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15881) [M::mem_pestat] mean and std.dev: (4119.34, 2816.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 333, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (334.97, 127.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1784, 3766, 6504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15944) [M::mem_pestat] mean and std.dev: (4213.88, 2807.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20664) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1662, 3648, 6381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15819) [M::mem_pestat] mean and std.dev: (4134.89, 2804.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20538) [M::mem_process_seqs] Processed 1333334 reads in 424.190 CPU sec, 26.978 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7547, 128750, 6994, 7451) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1616, 3695, 6433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16067) [M::mem_pestat] mean and std.dev: (4153.89, 2846.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 332, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (334.92, 127.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1872, 3862, 6459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15633) [M::mem_pestat] mean and std.dev: (4250.65, 2762.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20220) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1657, 3654, 6361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15769) [M::mem_pestat] mean and std.dev: (4127.87, 2822.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20473) [M::mem_process_seqs] Processed 1333334 reads in 424.926 CPU sec, 27.037 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7357, 129745, 6825, 7111) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1605, 3691, 6344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15822) [M::mem_pestat] mean and std.dev: (4121.94, 2816.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 333, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (335.34, 128.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1904, 3907, 6534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15794) [M::mem_pestat] mean and std.dev: (4294.99, 2785.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20424) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1649, 3636, 6271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15515) [M::mem_pestat] mean and std.dev: (4111.71, 2803.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20137) [M::mem_process_seqs] Processed 1333334 reads in 405.855 CPU sec, 25.930 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7642, 129793, 6721, 7406) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1557, 3571, 6294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15768) [M::mem_pestat] mean and std.dev: (4065.11, 2834.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 333, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (336.25, 128.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1874, 3865, 6617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16103) [M::mem_pestat] mean and std.dev: (4281.66, 2799.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20846) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1631, 3678, 6401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15941) [M::mem_pestat] mean and std.dev: (4132.29, 2812.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20711) [M::mem_process_seqs] Processed 1333334 reads in 416.542 CPU sec, 26.611 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7400, 128229, 6620, 7374) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1593, 3713, 6290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15684) [M::mem_pestat] mean and std.dev: (4081.80, 2815.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 333, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (335.67, 128.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1841, 3874, 6561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16001) [M::mem_pestat] mean and std.dev: (4267.81, 2803.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20721) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1677, 3778, 6428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15930) [M::mem_pestat] mean and std.dev: (4199.23, 2825.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20681) [M::mem_process_seqs] Processed 1333334 reads in 432.013 CPU sec, 27.414 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7361, 130000, 6961, 7279) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1579, 3650, 6402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16048) [M::mem_pestat] mean and std.dev: (4106.73, 2837.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20871) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 335, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (336.74, 128.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1878, 3943, 6610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16074) [M::mem_pestat] mean and std.dev: (4333.72, 2811.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20806) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1661, 3699, 6329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15665) [M::mem_pestat] mean and std.dev: (4133.05, 2789.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20333) [M::mem_process_seqs] Processed 1333334 reads in 407.879 CPU sec, 25.986 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7495, 130073, 6714, 7443) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1604, 3730, 6363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15881) [M::mem_pestat] mean and std.dev: (4120.71, 2824.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 334, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (336.21, 128.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1817, 3930, 6596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16154) [M::mem_pestat] mean and std.dev: (4299.71, 2802.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20933) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1641, 3712, 6286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15576) [M::mem_pestat] mean and std.dev: (4113.63, 2794.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20221) [M::mem_process_seqs] Processed 1333334 reads in 415.311 CPU sec, 26.356 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7607, 128717, 6807, 7348) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1572, 3635, 6298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15750) [M::mem_pestat] mean and std.dev: (4066.46, 2810.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 333, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (336.36, 128.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1759, 3872, 6466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15880) [M::mem_pestat] mean and std.dev: (4234.58, 2804.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20587) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1705, 3745, 6399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15787) [M::mem_pestat] mean and std.dev: (4183.16, 2819.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20481) [M::mem_process_seqs] Processed 1333334 reads in 433.179 CPU sec, 27.532 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7417, 127711, 6738, 7380) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1619, 3646, 6442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16088) [M::mem_pestat] mean and std.dev: (4138.35, 2845.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (246, 332, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (335.01, 127.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1883, 3866, 6479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15671) [M::mem_pestat] mean and std.dev: (4241.93, 2771.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20267) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1670, 3763, 6514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16202) [M::mem_pestat] mean and std.dev: (4207.30, 2852.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21046) [M::mem_process_seqs] Processed 1333334 reads in 451.809 CPU sec, 28.666 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7467, 128914, 6855, 7373) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1600, 3662, 6417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16051) [M::mem_pestat] mean and std.dev: (4125.30, 2850.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 335, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (337.36, 128.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1823, 3932, 6568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16058) [M::mem_pestat] mean and std.dev: (4281.56, 2812.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20803) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1731, 3825, 6460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15918) [M::mem_pestat] mean and std.dev: (4215.89, 2820.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20647) [M::mem_process_seqs] Processed 1333334 reads in 417.406 CPU sec, 26.553 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7501, 128439, 6792, 7250) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1622, 3693, 6371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15869) [M::mem_pestat] mean and std.dev: (4131.68, 2824.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 335, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (337.62, 129.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1842, 3936, 6669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16323) [M::mem_pestat] mean and std.dev: (4311.29, 2803.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21150) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1646, 3637, 6407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15929) [M::mem_pestat] mean and std.dev: (4130.10, 2811.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20690) [M::mem_process_seqs] Processed 1333334 reads in 422.466 CPU sec, 27.105 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7635, 128725, 6728, 7311) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1654, 3777, 6401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15895) [M::mem_pestat] mean and std.dev: (4153.46, 2805.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 335, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (337.82, 129.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1843, 3889, 6562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16000) [M::mem_pestat] mean and std.dev: (4275.68, 2810.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20719) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1645, 3612, 6296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15598) [M::mem_pestat] mean and std.dev: (4103.27, 2811.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20249) [M::mem_process_seqs] Processed 1333334 reads in 426.841 CPU sec, 27.186 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7469, 128583, 6720, 7335) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1636, 3738, 6488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16192) [M::mem_pestat] mean and std.dev: (4151.51, 2826.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 336, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (338.07, 129.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1851, 3813, 6511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15831) [M::mem_pestat] mean and std.dev: (4255.20, 2790.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20491) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1656, 3728, 6294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15570) [M::mem_pestat] mean and std.dev: (4125.83, 2780.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20208) [M::mem_process_seqs] Processed 1333334 reads in 415.675 CPU sec, 26.516 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7499, 127759, 6813, 7239) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1680, 3770, 6476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16068) [M::mem_pestat] mean and std.dev: (4183.92, 2822.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 334, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (336.57, 128.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1825, 3926, 6541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15973) [M::mem_pestat] mean and std.dev: (4270.94, 2786.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20689) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1619, 3706, 6406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15980) [M::mem_pestat] mean and std.dev: (4126.89, 2819.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20767) [M::mem_process_seqs] Processed 1333334 reads in 426.885 CPU sec, 27.202 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7440, 128583, 6715, 7264) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1609, 3662, 6249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15529) [M::mem_pestat] mean and std.dev: (4074.65, 2802.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20169) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 334, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (336.41, 128.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1795, 3781, 6543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16039) [M::mem_pestat] mean and std.dev: (4214.95, 2814.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20787) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1617, 3623, 6325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15741) [M::mem_pestat] mean and std.dev: (4116.83, 2826.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20449) [M::mem_process_seqs] Processed 1333334 reads in 435.683 CPU sec, 27.807 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7562, 128398, 6864, 7262) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1644, 3705, 6437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16023) [M::mem_pestat] mean and std.dev: (4134.01, 2818.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 335, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (337.33, 128.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1793, 3854, 6515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15959) [M::mem_pestat] mean and std.dev: (4242.27, 2810.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20681) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1681, 3769, 6421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15901) [M::mem_pestat] mean and std.dev: (4182.14, 2822.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20641) [M::mem_process_seqs] Processed 1333334 reads in 403.363 CPU sec, 25.684 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7495, 129084, 6729, 7205) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1666, 3715, 6379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15805) [M::mem_pestat] mean and std.dev: (4130.64, 2803.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 336, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (338.01, 129.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1847, 3930, 6604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16118) [M::mem_pestat] mean and std.dev: (4324.15, 2820.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20875) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1637, 3663, 6342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15752) [M::mem_pestat] mean and std.dev: (4105.96, 2799.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20457) [M::mem_process_seqs] Processed 1333334 reads in 405.962 CPU sec, 25.940 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7606, 128236, 6775, 7243) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1668, 3688, 6376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15792) [M::mem_pestat] mean and std.dev: (4129.66, 2813.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 336, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (338.53, 129.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1855, 3848, 6551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15943) [M::mem_pestat] mean and std.dev: (4267.21, 2799.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20639) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1723, 3850, 6533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16153) [M::mem_pestat] mean and std.dev: (4247.60, 2831.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20963) [M::mem_process_seqs] Processed 1333334 reads in 401.828 CPU sec, 25.610 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7520, 128281, 6865, 7363) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1621, 3630, 6445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16093) [M::mem_pestat] mean and std.dev: (4131.77, 2843.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 337, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (338.60, 129.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1829, 3907, 6519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15899) [M::mem_pestat] mean and std.dev: (4278.96, 2796.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20589) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1680, 3624, 6293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15519) [M::mem_pestat] mean and std.dev: (4114.66, 2779.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20132) [M::mem_process_seqs] Processed 1333334 reads in 412.380 CPU sec, 26.227 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7379, 127773, 6803, 7316) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1641, 3691, 6355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15783) [M::mem_pestat] mean and std.dev: (4116.17, 2812.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 335, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (337.92, 129.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1769, 3844, 6514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16004) [M::mem_pestat] mean and std.dev: (4225.38, 2804.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20749) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1647, 3660, 6454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16068) [M::mem_pestat] mean and std.dev: (4167.03, 2846.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20875) [M::mem_process_seqs] Processed 1333334 reads in 427.234 CPU sec, 27.099 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7604, 127652, 6820, 7274) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1655, 3757, 6351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15743) [M::mem_pestat] mean and std.dev: (4136.99, 2796.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20439) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 337, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (339.78, 130.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1825, 3818, 6565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16045) [M::mem_pestat] mean and std.dev: (4237.34, 2790.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20785) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1670, 3727, 6446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15998) [M::mem_pestat] mean and std.dev: (4168.66, 2818.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20774) [M::mem_process_seqs] Processed 1333334 reads in 413.312 CPU sec, 26.365 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7570, 127299, 6870, 7164) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1616, 3614, 6339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15785) [M::mem_pestat] mean and std.dev: (4095.42, 2808.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 336, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (339.06, 129.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1823, 3763, 6432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15650) [M::mem_pestat] mean and std.dev: (4210.35, 2783.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20259) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1640, 3691, 6385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15875) [M::mem_pestat] mean and std.dev: (4133.30, 2819.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20620) [M::mem_process_seqs] Processed 1333334 reads in 418.475 CPU sec, 26.631 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7541, 128162, 6789, 7373) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1655, 3724, 6529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16277) [M::mem_pestat] mean and std.dev: (4190.24, 2851.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21151) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 337, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (339.50, 130.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1856, 3906, 6480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15728) [M::mem_pestat] mean and std.dev: (4262.97, 2793.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20352) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1651, 3695, 6442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16024) [M::mem_pestat] mean and std.dev: (4140.68, 2814.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20815) [M::mem_process_seqs] Processed 1333334 reads in 406.281 CPU sec, 25.807 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7392, 128219, 6848, 7219) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1657, 3666, 6325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15661) [M::mem_pestat] mean and std.dev: (4131.56, 2808.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20329) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 336, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (338.67, 129.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1880, 3935, 6554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15902) [M::mem_pestat] mean and std.dev: (4301.71, 2803.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20576) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1695, 3799, 6378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15744) [M::mem_pestat] mean and std.dev: (4183.57, 2785.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20427) [M::mem_process_seqs] Processed 1333334 reads in 400.320 CPU sec, 25.458 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7496, 127632, 6863, 7252) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1631, 3645, 6261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15521) [M::mem_pestat] mean and std.dev: (4093.35, 2824.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20151) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 338, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (340.29, 130.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1886, 3883, 6521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15791) [M::mem_pestat] mean and std.dev: (4284.75, 2782.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20426) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1657, 3687, 6419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15943) [M::mem_pestat] mean and std.dev: (4143.07, 2819.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20705) [M::mem_process_seqs] Processed 1333334 reads in 406.437 CPU sec, 25.812 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7485, 125672, 6782, 7345) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1638, 3754, 6352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15780) [M::mem_pestat] mean and std.dev: (4139.66, 2798.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (247, 335, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (337.45, 129.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1852, 3825, 6563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15985) [M::mem_pestat] mean and std.dev: (4276.21, 2793.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20696) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1716, 3725, 6394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15750) [M::mem_pestat] mean and std.dev: (4194.21, 2815.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20428) [M::mem_process_seqs] Processed 1333334 reads in 458.516 CPU sec, 29.168 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7574, 127444, 6787, 7463) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1582, 3665, 6434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16138) [M::mem_pestat] mean and std.dev: (4132.91, 2852.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20990) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 339, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (340.17, 130.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1860, 3828, 6488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15744) [M::mem_pestat] mean and std.dev: (4246.32, 2774.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20372) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1693, 3734, 6543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16243) [M::mem_pestat] mean and std.dev: (4201.80, 2842.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21093) [M::mem_process_seqs] Processed 1333334 reads in 402.435 CPU sec, 25.619 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7440, 127209, 6742, 7340) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1641, 3626, 6398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15912) [M::mem_pestat] mean and std.dev: (4125.87, 2834.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 338, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (339.85, 130.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1829, 3836, 6466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15740) [M::mem_pestat] mean and std.dev: (4229.60, 2772.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20377) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1665, 3710, 6471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16083) [M::mem_pestat] mean and std.dev: (4188.06, 2851.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20889) [M::mem_process_seqs] Processed 1333334 reads in 400.839 CPU sec, 25.438 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7511, 126171, 6623, 7325) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1635, 3645, 6428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16014) [M::mem_pestat] mean and std.dev: (4138.40, 2854.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 339, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (340.50, 131.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1860, 3944, 6714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16422) [M::mem_pestat] mean and std.dev: (4343.16, 2825.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21276) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1729, 3788, 6405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15757) [M::mem_pestat] mean and std.dev: (4183.79, 2804.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20433) [M::mem_process_seqs] Processed 1333334 reads in 406.012 CPU sec, 25.786 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7575, 127849, 6812, 7219) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1603, 3578, 6408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16018) [M::mem_pestat] mean and std.dev: (4094.21, 2837.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 337, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (339.85, 130.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1810, 3912, 6573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16099) [M::mem_pestat] mean and std.dev: (4304.61, 2810.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1737, 3815, 6564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16218) [M::mem_pestat] mean and std.dev: (4215.68, 2834.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21045) [M::mem_process_seqs] Processed 1333334 reads in 415.002 CPU sec, 26.441 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7654, 127480, 6836, 7314) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1669, 3788, 6543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16291) [M::mem_pestat] mean and std.dev: (4213.42, 2870.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21165) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (249, 338, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (340.19, 130.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1842, 3876, 6534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15918) [M::mem_pestat] mean and std.dev: (4264.36, 2792.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20610) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1652, 3657, 6434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15998) [M::mem_pestat] mean and std.dev: (4173.91, 2864.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20780) [M::mem_process_seqs] Processed 1333334 reads in 409.270 CPU sec, 26.042 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7576, 127943, 7050, 7574) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1558, 3661, 6382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16030) [M::mem_pestat] mean and std.dev: (4113.88, 2842.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (250, 339, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (340.97, 131.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1848, 3931, 6550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15954) [M::mem_pestat] mean and std.dev: (4291.32, 2814.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20656) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1696, 3776, 6427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15889) [M::mem_pestat] mean and std.dev: (4183.74, 2800.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20620) [M::mem_process_seqs] Processed 1333334 reads in 400.183 CPU sec, 25.479 real sec [M::process] read 626434 sequences (93965100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7624, 128092, 6745, 7293) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1644, 3719, 6433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16011) [M::mem_pestat] mean and std.dev: (4148.15, 2820.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 337, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (339.91, 131.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1794, 3855, 6552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16068) [M::mem_pestat] mean and std.dev: (4254.92, 2799.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20826) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1653, 3690, 6405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15909) [M::mem_pestat] mean and std.dev: (4161.31, 2825.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20661) [M::mem_process_seqs] Processed 1333334 reads in 404.760 CPU sec, 25.700 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3572, 59784, 3263, 3365) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1640, 3706, 6284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15572) [M::mem_pestat] mean and std.dev: (4126.05, 2812.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20216) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (248, 336, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (337.55, 129.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1891, 3919, 6520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15778) [M::mem_pestat] mean and std.dev: (4275.53, 2772.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20407) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1715, 3745, 6326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15548) [M::mem_pestat] mean and std.dev: (4148.43, 2788.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20159) [M::mem_process_seqs] Processed 626434 reads in 200.233 CPU sec, 12.846 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -SP5M -t 20 /oak/stanford/groups/akundaje/marinovg/genomes/Ciliates/Tetrahymena_thermophila_SB210-E-JCVI_TeTherMIC_3/bwa-indexes/Tetrahymena_thermophila_SB210-E-JCVI_TeTherMIC_3.fa /oak/stanford/groups/akundaje/marinovg/papers/2022_Tetrahymena-Hi-C/2022-05-24-figures/juicer-Smc1-HA_1.5_hpm_HiChIP_all-MIC/splits/Smc1-HA_1.5_hpm_HiChIP_all_R1.fastq.gz /oak/stanford/groups/akundaje/marinovg/papers/2022_Tetrahymena-Hi-C/2022-05-24-figures/juicer-Smc1-HA_1.5_hpm_HiChIP_all-MIC/splits/Smc1-HA_1.5_hpm_HiChIP_all_R2.fastq.gz [main] Real time: 12087.133 sec; CPU: 188583.341 sec Picked up _JAVA_OPTIONS: -Xmx16384m Iterated 1000 times, returning estimate Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m