[M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 1600000 sequences (200000000 bp)... [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14694, 152273, 11485, 12047) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (770, 2993, 6112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16796) [M::mem_pestat] mean and std.dev: (3591.56, 3023.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22138) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.71, 80.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1816, 4021, 6673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16387) [M::mem_pestat] mean and std.dev: (4354.18, 2780.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21244) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1785, 3982, 6636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16338) [M::mem_pestat] mean and std.dev: (4305.69, 2819.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21189) [M::mem_process_seqs] Processed 1600000 reads in 584.738 CPU sec, 80.421 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14314, 152651, 11492, 12149) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (800, 3011, 6058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16574) [M::mem_pestat] mean and std.dev: (3580.66, 2998.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.54, 81.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1783, 3922, 6466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15832) [M::mem_pestat] mean and std.dev: (4281.61, 2757.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20515) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1725, 3867, 6496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16038) [M::mem_pestat] mean and std.dev: (4229.80, 2809.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20809) [M::mem_process_seqs] Processed 1600000 reads in 510.567 CPU sec, 34.463 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13756, 153807, 11760, 12135) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1118, 3270, 6242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16490) [M::mem_pestat] mean and std.dev: (3774.12, 2972.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.47, 80.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1812, 4015, 6584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16128) [M::mem_pestat] mean and std.dev: (4342.46, 2755.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20900) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1761, 3930, 6518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16032) [M::mem_pestat] mean and std.dev: (4250.46, 2804.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20789) [M::mem_process_seqs] Processed 1600000 reads in 501.692 CPU sec, 32.419 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13262, 154265, 11756, 12008) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1336, 3463, 6351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16381) [M::mem_pestat] mean and std.dev: (3917.25, 2924.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.49, 80.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1800, 4028, 6595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16185) [M::mem_pestat] mean and std.dev: (4334.73, 2767.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20980) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1739, 3895, 6536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16130) [M::mem_pestat] mean and std.dev: (4252.97, 2812.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20927) [M::mem_process_seqs] Processed 1600000 reads in 487.256 CPU sec, 31.182 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12955, 154354, 11667, 12418) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1436, 3713, 6428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16412) [M::mem_pestat] mean and std.dev: (4040.74, 2908.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.43, 80.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1828, 3975, 6518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15898) [M::mem_pestat] mean and std.dev: (4321.18, 2760.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20588) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1770, 3932, 6588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16224) [M::mem_pestat] mean and std.dev: (4285.71, 2833.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21042) [M::mem_process_seqs] Processed 1600000 reads in 481.462 CPU sec, 30.921 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12930, 156122, 11667, 12032) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1490, 3712, 6423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16289) [M::mem_pestat] mean and std.dev: (4071.14, 2895.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21222) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.80, 80.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1817, 4020, 6615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16211) [M::mem_pestat] mean and std.dev: (4345.16, 2782.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21009) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1686, 3902, 6500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16128) [M::mem_pestat] mean and std.dev: (4229.04, 2827.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20942) [M::mem_process_seqs] Processed 1600000 reads in 473.691 CPU sec, 30.346 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12667, 155298, 11682, 12066) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1492, 3732, 6431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16309) [M::mem_pestat] mean and std.dev: (4064.64, 2885.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21248) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.51, 80.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1810, 3948, 6534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15982) [M::mem_pestat] mean and std.dev: (4312.84, 2769.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20706) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1773, 3969, 6496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15942) [M::mem_pestat] mean and std.dev: (4253.13, 2789.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20665) [M::mem_process_seqs] Processed 1600000 reads in 469.907 CPU sec, 30.044 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13006, 155646, 11770, 12172) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1461, 3655, 6420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16338) [M::mem_pestat] mean and std.dev: (4035.69, 2888.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21297) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (357.87, 80.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1807, 3986, 6539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16003) [M::mem_pestat] mean and std.dev: (4326.54, 2779.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20735) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1698, 3895, 6586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16362) [M::mem_pestat] mean and std.dev: (4238.96, 2840.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21250) [M::mem_process_seqs] Processed 1600000 reads in 467.290 CPU sec, 29.884 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12760, 156024, 11640, 12162) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1459, 3642, 6393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16261) [M::mem_pestat] mean and std.dev: (4029.74, 2886.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21195) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (307, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 667) [M::mem_pestat] mean and std.dev: (357.33, 80.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1842, 4019, 6629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16203) [M::mem_pestat] mean and std.dev: (4367.95, 2789.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20990) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1731, 3982, 6578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16272) [M::mem_pestat] mean and std.dev: (4262.31, 2829.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21119) [M::mem_process_seqs] Processed 1600000 reads in 466.716 CPU sec, 29.997 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12686, 155476, 11666, 12276) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1531, 3683, 6402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16144) [M::mem_pestat] mean and std.dev: (4070.88, 2881.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (307, 361, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 664) [M::mem_pestat] mean and std.dev: (357.21, 80.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1785, 3953, 6552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16086) [M::mem_pestat] mean and std.dev: (4312.55, 2802.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20853) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1705, 3910, 6575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16315) [M::mem_pestat] mean and std.dev: (4249.47, 2830.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21185) [M::mem_process_seqs] Processed 1600000 reads in 470.180 CPU sec, 30.085 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12847, 156121, 11611, 12094) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1450, 3696, 6413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16339) [M::mem_pestat] mean and std.dev: (4052.46, 2903.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21302) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (307, 361, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 664) [M::mem_pestat] mean and std.dev: (356.79, 80.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1821, 3982, 6583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16107) [M::mem_pestat] mean and std.dev: (4351.33, 2791.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20869) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1704, 3955, 6533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16191) [M::mem_pestat] mean and std.dev: (4236.69, 2827.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21020) [M::mem_process_seqs] Processed 1600000 reads in 467.755 CPU sec, 30.100 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14676, 151433, 11483, 12289) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (672, 2824, 5929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16443) [M::mem_pestat] mean and std.dev: (3502.50, 3008.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (358.72, 80.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1779, 3960, 6466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15840) [M::mem_pestat] mean and std.dev: (4285.37, 2758.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20527) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1770, 3907, 6521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16023) [M::mem_pestat] mean and std.dev: (4239.92, 2788.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20774) [M::mem_process_seqs] Processed 1600000 reads in 525.925 CPU sec, 35.115 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14499, 153328, 11710, 12286) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (880, 3048, 6136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16648) [M::mem_pestat] mean and std.dev: (3628.84, 3006.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21904) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.44, 80.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1765, 4003, 6539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16087) [M::mem_pestat] mean and std.dev: (4316.12, 2793.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20861) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1732, 3937, 6548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16180) [M::mem_pestat] mean and std.dev: (4255.46, 2816.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20996) [M::mem_process_seqs] Processed 1600000 reads in 517.339 CPU sec, 33.493 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13427, 153088, 11715, 12312) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1154, 3360, 6275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16517) [M::mem_pestat] mean and std.dev: (3823.27, 2977.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.87, 80.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1793, 3991, 6528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15998) [M::mem_pestat] mean and std.dev: (4316.19, 2758.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20733) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1756, 3945, 6515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16033) [M::mem_pestat] mean and std.dev: (4255.78, 2807.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20792) [M::mem_process_seqs] Processed 1600000 reads in 501.703 CPU sec, 32.116 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13095, 153899, 11891, 12301) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1365, 3571, 6360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16350) [M::mem_pestat] mean and std.dev: (3951.49, 2901.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.54, 80.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1812, 3994, 6569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16083) [M::mem_pestat] mean and std.dev: (4333.69, 2774.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20840) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1740, 3927, 6520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16080) [M::mem_pestat] mean and std.dev: (4246.87, 2813.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20860) [M::mem_process_seqs] Processed 1600000 reads in 490.955 CPU sec, 31.483 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12829, 153340, 11579, 12337) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1389, 3612, 6381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16365) [M::mem_pestat] mean and std.dev: (3994.08, 2899.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.31, 80.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1798, 3957, 6532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16000) [M::mem_pestat] mean and std.dev: (4300.72, 2753.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20734) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1760, 3958, 6526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16058) [M::mem_pestat] mean and std.dev: (4261.20, 2806.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20824) [M::mem_process_seqs] Processed 1600000 reads in 493.704 CPU sec, 31.659 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12927, 154704, 11537, 11961) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1480, 3691, 6403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16249) [M::mem_pestat] mean and std.dev: (4046.82, 2893.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21172) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (66, 671) [M::mem_pestat] mean and std.dev: (358.67, 80.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1818, 3963, 6519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15921) [M::mem_pestat] mean and std.dev: (4319.25, 2769.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20622) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1763, 3929, 6543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16103) [M::mem_pestat] mean and std.dev: (4253.88, 2814.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20883) [M::mem_process_seqs] Processed 1600000 reads in 481.574 CPU sec, 30.816 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12843, 154784, 11501, 11891) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1441, 3622, 6431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16411) [M::mem_pestat] mean and std.dev: (4029.54, 2900.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (358.17, 80.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1860, 4027, 6596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16068) [M::mem_pestat] mean and std.dev: (4378.65, 2777.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20804) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1705, 3919, 6455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15955) [M::mem_pestat] mean and std.dev: (4207.75, 2801.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20705) [M::process] read 1600000 sequences (200000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 476.834 CPU sec, 30.512 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12914, 155599, 11651, 12117) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1479, 3659, 6393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16221) [M::mem_pestat] mean and std.dev: (4040.75, 2887.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21135) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (357.79, 80.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1832, 4011, 6627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16217) [M::mem_pestat] mean and std.dev: (4355.01, 2781.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21012) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1709, 3902, 6521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16145) [M::mem_pestat] mean and std.dev: (4229.19, 2817.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20957) [M::mem_process_seqs] Processed 1600000 reads in 471.179 CPU sec, 30.259 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12809, 155910, 11629, 12321) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1477, 3687, 6398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16240) [M::mem_pestat] mean and std.dev: (4039.29, 2892.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (357.48, 80.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1822, 4033, 6597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16147) [M::mem_pestat] mean and std.dev: (4349.56, 2774.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20922) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1754, 3936, 6563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16181) [M::mem_pestat] mean and std.dev: (4261.90, 2822.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20990) [M::mem_process_seqs] Processed 1600000 reads in 466.698 CPU sec, 29.942 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12656, 155445, 11597, 12306) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1503, 3773, 6474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16416) [M::mem_pestat] mean and std.dev: (4093.90, 2882.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (307, 361, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 664) [M::mem_pestat] mean and std.dev: (357.17, 80.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1865, 3985, 6578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16004) [M::mem_pestat] mean and std.dev: (4352.76, 2781.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20717) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1771, 4013, 6610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16288) [M::mem_pestat] mean and std.dev: (4300.79, 2836.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21127) [M::mem_process_seqs] Processed 1600000 reads in 473.891 CPU sec, 30.374 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13455, 155830, 11489, 11982) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1327, 3565, 6363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16435) [M::mem_pestat] mean and std.dev: (3943.96, 2929.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (357.62, 80.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1814, 3971, 6627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16253) [M::mem_pestat] mean and std.dev: (4343.06, 2787.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21066) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1710, 3898, 6530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16170) [M::mem_pestat] mean and std.dev: (4239.45, 2825.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20990) [M::mem_process_seqs] Processed 1600000 reads in 472.055 CPU sec, 30.392 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14956, 152936, 11575, 12140) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (457, 2708, 5917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16837) [M::mem_pestat] mean and std.dev: (3409.01, 3025.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22297) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (359.00, 81.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1796, 3964, 6478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15842) [M::mem_pestat] mean and std.dev: (4302.43, 2756.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20524) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1751, 3912, 6496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15986) [M::mem_pestat] mean and std.dev: (4242.02, 2801.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20731) [M::mem_process_seqs] Processed 1600000 reads in 510.321 CPU sec, 33.310 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14504, 152918, 11711, 12097) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (686, 2905, 6000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16628) [M::mem_pestat] mean and std.dev: (3524.00, 3003.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21942) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (359.16, 81.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1792, 4040, 6632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16312) [M::mem_pestat] mean and std.dev: (4360.78, 2788.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21152) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1731, 3872, 6541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16161) [M::mem_pestat] mean and std.dev: (4237.44, 2815.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20971) [M::mem_process_seqs] Processed 1600000 reads in 501.437 CPU sec, 32.329 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14021, 153818, 11408, 12135) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1093, 3286, 6163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16303) [M::mem_pestat] mean and std.dev: (3749.53, 2951.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (359.11, 81.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1798, 3977, 6568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16108) [M::mem_pestat] mean and std.dev: (4313.63, 2778.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20878) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1748, 3878, 6573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16223) [M::mem_pestat] mean and std.dev: (4253.51, 2822.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21048) [M::mem_process_seqs] Processed 1600000 reads in 489.021 CPU sec, 31.279 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13107, 155332, 11504, 12109) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1317, 3475, 6298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16260) [M::mem_pestat] mean and std.dev: (3910.41, 2924.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.96, 81.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1814, 3977, 6553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16031) [M::mem_pestat] mean and std.dev: (4317.86, 2763.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20770) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1799, 3962, 6533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16001) [M::mem_pestat] mean and std.dev: (4273.58, 2798.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20735) [M::mem_process_seqs] Processed 1600000 reads in 477.950 CPU sec, 30.614 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12947, 155406, 11275, 12156) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1386, 3553, 6317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16179) [M::mem_pestat] mean and std.dev: (3962.01, 2888.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21110) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (358.67, 81.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1818, 4008, 6591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16137) [M::mem_pestat] mean and std.dev: (4343.60, 2778.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20910) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1785, 3967, 6538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16044) [M::mem_pestat] mean and std.dev: (4268.65, 2796.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20797) [M::mem_process_seqs] Processed 1600000 reads in 467.753 CPU sec, 29.924 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12750, 155608, 11524, 12008) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1450, 3616, 6413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16339) [M::mem_pestat] mean and std.dev: (4018.74, 2896.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21302) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.48, 80.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1765, 3957, 6582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16216) [M::mem_pestat] mean and std.dev: (4297.23, 2801.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21033) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1760, 3962, 6578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16214) [M::mem_pestat] mean and std.dev: (4272.01, 2813.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21032) [M::mem_process_seqs] Processed 1600000 reads in 465.565 CPU sec, 29.833 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12726, 155780, 11619, 12111) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1478, 3655, 6402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16250) [M::mem_pestat] mean and std.dev: (4045.27, 2885.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21174) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.52, 80.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1827, 3973, 6551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15999) [M::mem_pestat] mean and std.dev: (4326.28, 2772.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20723) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1730, 3931, 6584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16292) [M::mem_pestat] mean and std.dev: (4255.58, 2838.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21146) [M::mem_process_seqs] Processed 1600000 reads in 462.053 CPU sec, 29.562 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12840, 156032, 11438, 12029) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1424, 3558, 6374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16274) [M::mem_pestat] mean and std.dev: (3993.76, 2887.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21224) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (357.67, 80.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1815, 4011, 6516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15918) [M::mem_pestat] mean and std.dev: (4329.22, 2767.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20619) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1761, 3939, 6542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16104) [M::mem_pestat] mean and std.dev: (4263.98, 2808.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20885) [M::mem_process_seqs] Processed 1600000 reads in 463.226 CPU sec, 29.685 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12512, 156076, 11568, 12015) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1436, 3671, 6418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16382) [M::mem_pestat] mean and std.dev: (4033.08, 2895.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (307, 362, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 670) [M::mem_pestat] mean and std.dev: (357.72, 81.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1782, 3978, 6621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16299) [M::mem_pestat] mean and std.dev: (4320.13, 2790.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21138) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1746, 3927, 6520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16068) [M::mem_pestat] mean and std.dev: (4244.73, 2806.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20842) [M::mem_process_seqs] Processed 1600000 reads in 463.863 CPU sec, 29.742 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12672, 156324, 11499, 12182) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1464, 3677, 6433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16371) [M::mem_pestat] mean and std.dev: (4051.80, 2903.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21340) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (307, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 667) [M::mem_pestat] mean and std.dev: (357.33, 81.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1793, 3923, 6581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16157) [M::mem_pestat] mean and std.dev: (4306.48, 2785.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20945) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1688, 3879, 6514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16166) [M::mem_pestat] mean and std.dev: (4220.61, 2833.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20992) [M::mem_process_seqs] Processed 1600000 reads in 465.209 CPU sec, 29.774 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13443, 155240, 11425, 12377) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1188, 3321, 6304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16536) [M::mem_pestat] mean and std.dev: (3813.74, 2949.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (357.95, 80.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1802, 3985, 6621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16259) [M::mem_pestat] mean and std.dev: (4336.91, 2787.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21078) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1720, 3902, 6570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16270) [M::mem_pestat] mean and std.dev: (4248.97, 2823.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21120) [M::mem_process_seqs] Processed 1600000 reads in 494.333 CPU sec, 31.600 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15549, 151950, 11710, 12181) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (341, 2509, 5849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16865) [M::mem_pestat] mean and std.dev: (3327.79, 3038.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (358.97, 81.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1766, 3995, 6615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16313) [M::mem_pestat] mean and std.dev: (4333.99, 2804.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21162) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1768, 3981, 6538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16078) [M::mem_pestat] mean and std.dev: (4272.90, 2813.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20848) [M::mem_process_seqs] Processed 1600000 reads in 542.818 CPU sec, 36.190 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14739, 153326, 11652, 12326) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (657, 2820, 5958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16560) [M::mem_pestat] mean and std.dev: (3480.35, 3004.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21861) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (359.03, 80.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1805, 3974, 6526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15968) [M::mem_pestat] mean and std.dev: (4315.44, 2764.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20689) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1801, 4025, 6591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16171) [M::mem_pestat] mean and std.dev: (4294.71, 2803.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20961) [M::mem_process_seqs] Processed 1600000 reads in 536.489 CPU sec, 34.903 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13959, 152935, 11859, 12190) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1126, 3258, 6167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16249) [M::mem_pestat] mean and std.dev: (3752.61, 2953.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21290) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 364, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (359.43, 80.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1839, 4056, 6626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16200) [M::mem_pestat] mean and std.dev: (4369.55, 2785.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20987) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1786, 3998, 6630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16318) [M::mem_pestat] mean and std.dev: (4311.01, 2819.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21162) [M::mem_process_seqs] Processed 1600000 reads in 509.695 CPU sec, 32.562 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13446, 153626, 11707, 12232) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1352, 3539, 6337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16307) [M::mem_pestat] mean and std.dev: (3939.74, 2929.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (359.27, 81.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1803, 3926, 6571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16107) [M::mem_pestat] mean and std.dev: (4307.27, 2771.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20875) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1773, 3948, 6564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16146) [M::mem_pestat] mean and std.dev: (4264.71, 2809.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20937) [M::mem_process_seqs] Processed 1600000 reads in 494.852 CPU sec, 31.731 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13019, 154944, 11667, 12385) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1407, 3654, 6395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16371) [M::mem_pestat] mean and std.dev: (4017.31, 2910.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (359.22, 81.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1825, 3997, 6537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15961) [M::mem_pestat] mean and std.dev: (4329.75, 2763.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20673) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1775, 3916, 6560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16130) [M::mem_pestat] mean and std.dev: (4244.35, 2814.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20915) [M::mem_process_seqs] Processed 1600000 reads in 487.179 CPU sec, 31.211 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12845, 154640, 11511, 12224) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1473, 3699, 6391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16227) [M::mem_pestat] mean and std.dev: (4025.63, 2872.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21145) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (359.04, 81.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1786, 3966, 6559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16105) [M::mem_pestat] mean and std.dev: (4316.32, 2791.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20878) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1730, 3961, 6562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16226) [M::mem_pestat] mean and std.dev: (4267.44, 2816.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21058) [M::mem_process_seqs] Processed 1600000 reads in 482.630 CPU sec, 30.878 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12947, 155780, 11694, 12220) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1448, 3667, 6367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16205) [M::mem_pestat] mean and std.dev: (4030.52, 2886.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21124) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (66, 671) [M::mem_pestat] mean and std.dev: (358.64, 81.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1816, 3941, 6464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15760) [M::mem_pestat] mean and std.dev: (4291.51, 2756.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20408) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1766, 3900, 6463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15857) [M::mem_pestat] mean and std.dev: (4224.37, 2797.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20554) [M::mem_process_seqs] Processed 1600000 reads in 471.389 CPU sec, 30.200 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12864, 155575, 11609, 12294) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1476, 3658, 6396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16236) [M::mem_pestat] mean and std.dev: (4038.51, 2890.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21156) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.48, 81.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1808, 4016, 6618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16238) [M::mem_pestat] mean and std.dev: (4352.11, 2787.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21048) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1790, 3991, 6622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16286) [M::mem_pestat] mean and std.dev: (4308.36, 2829.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21118) [M::mem_process_seqs] Processed 1600000 reads in 478.132 CPU sec, 30.655 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12798, 155985, 11458, 12082) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1473, 3649, 6386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16212) [M::mem_pestat] mean and std.dev: (4048.24, 2882.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21125) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (358.11, 80.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1808, 3937, 6508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15908) [M::mem_pestat] mean and std.dev: (4311.77, 2775.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20608) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1729, 3907, 6538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16156) [M::mem_pestat] mean and std.dev: (4242.83, 2823.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20965) [M::mem_process_seqs] Processed 1600000 reads in 472.759 CPU sec, 30.429 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12850, 155777, 11722, 12167) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1480, 3744, 6410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16270) [M::mem_pestat] mean and std.dev: (4061.08, 2881.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21200) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (357.84, 80.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1769, 3901, 6445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15797) [M::mem_pestat] mean and std.dev: (4262.11, 2763.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20473) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1781, 3966, 6581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16181) [M::mem_pestat] mean and std.dev: (4290.84, 2841.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20981) [M::mem_process_seqs] Processed 1600000 reads in 474.915 CPU sec, 30.374 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14711, 153340, 11603, 12301) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (700, 2965, 6031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16693) [M::mem_pestat] mean and std.dev: (3549.91, 3015.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22024) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.36, 81.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1827, 4054, 6685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16401) [M::mem_pestat] mean and std.dev: (4382.39, 2786.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21259) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1770, 3936, 6533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16059) [M::mem_pestat] mean and std.dev: (4263.08, 2804.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20822) [M::mem_process_seqs] Processed 1600000 reads in 524.124 CPU sec, 33.626 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15213, 151962, 11610, 12271) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (433, 2596, 5878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16768) [M::mem_pestat] mean and std.dev: (3382.95, 3037.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22213) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (310, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (72, 667) [M::mem_pestat] mean and std.dev: (359.45, 80.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1819, 4044, 6533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15961) [M::mem_pestat] mean and std.dev: (4353.10, 2772.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20675) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1768, 3940, 6503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15973) [M::mem_pestat] mean and std.dev: (4253.76, 2792.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20708) [M::mem_process_seqs] Processed 1600000 reads in 540.170 CPU sec, 34.728 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14544, 153096, 11694, 12168) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (825, 2984, 6076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16578) [M::mem_pestat] mean and std.dev: (3584.47, 2993.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (358.71, 80.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1785, 3989, 6569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16137) [M::mem_pestat] mean and std.dev: (4312.43, 2776.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20921) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1758, 3977, 6507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16005) [M::mem_pestat] mean and std.dev: (4261.16, 2796.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20754) [M::mem_process_seqs] Processed 1600000 reads in 540.508 CPU sec, 34.549 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13534, 153295, 11835, 12199) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1199, 3380, 6280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16442) [M::mem_pestat] mean and std.dev: (3830.17, 2942.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (358.97, 80.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1810, 4038, 6637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16291) [M::mem_pestat] mean and std.dev: (4357.66, 2791.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21118) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1743, 3972, 6567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16215) [M::mem_pestat] mean and std.dev: (4263.00, 2814.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21039) [M::mem_process_seqs] Processed 1600000 reads in 516.366 CPU sec, 32.990 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13095, 153401, 11718, 12042) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1340, 3515, 6301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16223) [M::mem_pestat] mean and std.dev: (3921.99, 2897.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21184) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.41, 80.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1819, 4002, 6570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16072) [M::mem_pestat] mean and std.dev: (4351.57, 2771.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20823) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1758, 3954, 6505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15999) [M::mem_pestat] mean and std.dev: (4261.90, 2801.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20746) [M::mem_process_seqs] Processed 1600000 reads in 517.844 CPU sec, 33.200 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13020, 155073, 11712, 12257) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1416, 3632, 6365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16263) [M::mem_pestat] mean and std.dev: (4000.39, 2892.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21212) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 364, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.94, 80.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1820, 3978, 6599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16157) [M::mem_pestat] mean and std.dev: (4340.68, 2784.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20936) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1733, 3906, 6580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16274) [M::mem_pestat] mean and std.dev: (4257.56, 2829.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21121) [M::mem_process_seqs] Processed 1600000 reads in 488.794 CPU sec, 31.298 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13027, 153399, 11717, 12037) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1473, 3644, 6388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16218) [M::mem_pestat] mean and std.dev: (4041.16, 2891.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21133) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.29, 81.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1778, 3984, 6540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16064) [M::mem_pestat] mean and std.dev: (4324.17, 2788.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20826) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1732, 3919, 6490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16006) [M::mem_pestat] mean and std.dev: (4233.53, 2816.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20764) [M::mem_process_seqs] Processed 1600000 reads in 500.783 CPU sec, 32.193 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12860, 155435, 11673, 12183) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1489, 3620, 6355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16087) [M::mem_pestat] mean and std.dev: (4022.37, 2861.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (358.01, 80.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1812, 3997, 6555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16041) [M::mem_pestat] mean and std.dev: (4328.16, 2779.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20784) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1704, 3899, 6550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16242) [M::mem_pestat] mean and std.dev: (4242.86, 2827.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21088) [M::mem_process_seqs] Processed 1600000 reads in 487.166 CPU sec, 31.159 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12883, 154663, 11606, 12283) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1499, 3639, 6407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16223) [M::mem_pestat] mean and std.dev: (4044.87, 2871.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21131) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (357.36, 80.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1795, 3956, 6547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16051) [M::mem_pestat] mean and std.dev: (4313.72, 2775.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20803) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1707, 3894, 6507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16107) [M::mem_pestat] mean and std.dev: (4215.70, 2811.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20907) [M::mem_process_seqs] Processed 1600000 reads in 488.654 CPU sec, 31.347 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14485, 153803, 11727, 12230) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (697, 2954, 6002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16612) [M::mem_pestat] mean and std.dev: (3524.82, 3001.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.35, 80.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1776, 3952, 6644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16380) [M::mem_pestat] mean and std.dev: (4337.82, 2795.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21248) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1752, 3961, 6578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16230) [M::mem_pestat] mean and std.dev: (4280.41, 2830.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21056) [M::mem_process_seqs] Processed 1600000 reads in 506.777 CPU sec, 32.634 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15961, 152320, 11628, 12219) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 2421, 5676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16598) [M::mem_pestat] mean and std.dev: (3242.54, 3028.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22059) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (359.12, 80.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1799, 3903, 6475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15827) [M::mem_pestat] mean and std.dev: (4277.06, 2753.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20503) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1739, 3845, 6478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15956) [M::mem_pestat] mean and std.dev: (4214.08, 2799.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20695) [M::mem_process_seqs] Processed 1600000 reads in 526.640 CPU sec, 33.782 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14860, 152412, 11633, 12100) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (575, 2689, 5929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16637) [M::mem_pestat] mean and std.dev: (3427.68, 3013.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21991) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 364, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (359.28, 80.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1814, 3996, 6597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16163) [M::mem_pestat] mean and std.dev: (4328.30, 2781.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20946) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1798, 3939, 6512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15940) [M::mem_pestat] mean and std.dev: (4266.56, 2785.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20654) [M::mem_process_seqs] Processed 1600000 reads in 512.680 CPU sec, 32.790 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13909, 154182, 11529, 12086) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1004, 3226, 6233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16691) [M::mem_pestat] mean and std.dev: (3729.99, 3003.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21920) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (359.19, 80.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1756, 3912, 6512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16024) [M::mem_pestat] mean and std.dev: (4278.99, 2785.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20780) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1777, 3945, 6563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16135) [M::mem_pestat] mean and std.dev: (4273.16, 2811.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20921) [M::mem_process_seqs] Processed 1600000 reads in 498.923 CPU sec, 31.914 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13255, 154964, 11643, 12245) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1268, 3431, 6305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16379) [M::mem_pestat] mean and std.dev: (3883.76, 2944.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 364, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (359.41, 81.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1770, 3928, 6582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16206) [M::mem_pestat] mean and std.dev: (4299.07, 2797.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21018) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1749, 3909, 6532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16098) [M::mem_pestat] mean and std.dev: (4248.11, 2810.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20881) [M::mem_process_seqs] Processed 1600000 reads in 483.313 CPU sec, 30.919 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13107, 155370, 11542, 12089) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1382, 3526, 6334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16238) [M::mem_pestat] mean and std.dev: (3951.74, 2901.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21190) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 362, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.39, 80.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1802, 3986, 6604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16208) [M::mem_pestat] mean and std.dev: (4331.00, 2777.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21010) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1782, 4014, 6537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16047) [M::mem_pestat] mean and std.dev: (4282.70, 2805.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20802) [M::mem_process_seqs] Processed 1600000 reads in 474.488 CPU sec, 30.469 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13010, 155019, 11660, 12085) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1387, 3594, 6375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16351) [M::mem_pestat] mean and std.dev: (3980.35, 2909.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (358.78, 80.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1827, 4034, 6645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16281) [M::mem_pestat] mean and std.dev: (4364.17, 2775.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21099) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1746, 3956, 6589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16275) [M::mem_pestat] mean and std.dev: (4263.56, 2823.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21118) [M::mem_process_seqs] Processed 1600000 reads in 474.974 CPU sec, 30.357 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12870, 155285, 11460, 12140) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1400, 3533, 6368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16304) [M::mem_pestat] mean and std.dev: (3969.35, 2902.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21272) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.48, 81.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1805, 3977, 6529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15977) [M::mem_pestat] mean and std.dev: (4311.59, 2774.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20701) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1717, 3911, 6487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16027) [M::mem_pestat] mean and std.dev: (4223.35, 2808.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20797) [M::mem_process_seqs] Processed 1600000 reads in 472.614 CPU sec, 30.187 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12825, 155777, 11511, 12162) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1470, 3733, 6513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16599) [M::mem_pestat] mean and std.dev: (4085.36, 2906.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.47, 80.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1775, 3979, 6557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16121) [M::mem_pestat] mean and std.dev: (4307.73, 2788.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20903) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1729, 3958, 6571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16255) [M::mem_pestat] mean and std.dev: (4274.44, 2836.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21097) [M::mem_process_seqs] Processed 1600000 reads in 480.266 CPU sec, 30.664 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12792, 155294, 11562, 12265) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1478, 3668, 6397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16235) [M::mem_pestat] mean and std.dev: (4037.03, 2871.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21154) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (357.70, 80.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1811, 3998, 6562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16064) [M::mem_pestat] mean and std.dev: (4329.41, 2765.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20815) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1753, 3918, 6548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16138) [M::mem_pestat] mean and std.dev: (4260.23, 2811.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20933) [M::mem_process_seqs] Processed 1600000 reads in 471.765 CPU sec, 30.393 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12629, 156045, 11522, 12150) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1493, 3743, 6451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16367) [M::mem_pestat] mean and std.dev: (4086.61, 2895.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (357.67, 80.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1782, 3964, 6640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16356) [M::mem_pestat] mean and std.dev: (4330.69, 2797.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1742, 3943, 6581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16259) [M::mem_pestat] mean and std.dev: (4267.77, 2840.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21098) [M::mem_process_seqs] Processed 1600000 reads in 468.874 CPU sec, 30.060 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14581, 153738, 11604, 12107) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (783, 3015, 6026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16512) [M::mem_pestat] mean and std.dev: (3567.27, 3008.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.63, 80.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1824, 3998, 6538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15966) [M::mem_pestat] mean and std.dev: (4322.13, 2769.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20680) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1743, 3954, 6527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16095) [M::mem_pestat] mean and std.dev: (4259.34, 2817.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20879) [M::mem_process_seqs] Processed 1600000 reads in 507.369 CPU sec, 32.513 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14525, 154441, 11651, 12012) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (835, 2974, 6043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16459) [M::mem_pestat] mean and std.dev: (3582.55, 2989.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.76, 80.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1840, 4074, 6692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16396) [M::mem_pestat] mean and std.dev: (4390.35, 2792.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21248) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1738, 3857, 6519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16081) [M::mem_pestat] mean and std.dev: (4231.07, 2820.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20862) [M::mem_process_seqs] Processed 1600000 reads in 503.002 CPU sec, 32.172 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13535, 155059, 11887, 12228) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1162, 3363, 6207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16297) [M::mem_pestat] mean and std.dev: (3803.14, 2944.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21342) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.36, 80.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1787, 3941, 6542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16052) [M::mem_pestat] mean and std.dev: (4300.11, 2781.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20807) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1715, 3947, 6557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16241) [M::mem_pestat] mean and std.dev: (4265.84, 2822.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21083) [M::mem_process_seqs] Processed 1600000 reads in 498.557 CPU sec, 32.037 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13222, 155200, 11529, 12403) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1331, 3505, 6357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16409) [M::mem_pestat] mean and std.dev: (3930.75, 2922.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21435) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.48, 80.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1804, 3942, 6590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16162) [M::mem_pestat] mean and std.dev: (4320.65, 2787.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20948) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1813, 3933, 6542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16000) [M::mem_pestat] mean and std.dev: (4273.26, 2795.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20729) [M::mem_process_seqs] Processed 1600000 reads in 480.742 CPU sec, 30.919 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12809, 156139, 11528, 12062) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1452, 3591, 6361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16179) [M::mem_pestat] mean and std.dev: (3998.07, 2889.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21088) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.47, 80.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1782, 3982, 6551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16089) [M::mem_pestat] mean and std.dev: (4312.78, 2791.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20858) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1707, 3931, 6548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16230) [M::mem_pestat] mean and std.dev: (4244.42, 2814.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21071) [M::mem_process_seqs] Processed 1600000 reads in 471.135 CPU sec, 30.238 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12867, 155880, 11593, 12233) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1432, 3631, 6404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16348) [M::mem_pestat] mean and std.dev: (4026.87, 2906.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (358.24, 80.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1828, 4005, 6612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16180) [M::mem_pestat] mean and std.dev: (4339.03, 2770.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20964) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1745, 3949, 6515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16055) [M::mem_pestat] mean and std.dev: (4246.59, 2810.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20825) [M::mem_process_seqs] Processed 1600000 reads in 468.171 CPU sec, 30.001 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12795, 156396, 11522, 12052) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1491, 3696, 6409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16245) [M::mem_pestat] mean and std.dev: (4057.44, 2886.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21163) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (358.13, 80.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1806, 4046, 6555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16053) [M::mem_pestat] mean and std.dev: (4357.01, 2786.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20802) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1705, 3938, 6513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16129) [M::mem_pestat] mean and std.dev: (4248.85, 2825.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20937) [M::mem_process_seqs] Processed 1600000 reads in 463.398 CPU sec, 29.686 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12976, 156499, 11779, 11966) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1413, 3597, 6413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16413) [M::mem_pestat] mean and std.dev: (4017.40, 2905.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.14, 80.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1797, 3993, 6608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16230) [M::mem_pestat] mean and std.dev: (4331.34, 2793.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21041) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1755, 3949, 6566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16188) [M::mem_pestat] mean and std.dev: (4262.94, 2824.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20999) [M::mem_process_seqs] Processed 1600000 reads in 467.467 CPU sec, 29.942 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12782, 155941, 11600, 12018) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1483, 3719, 6549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16681) [M::mem_pestat] mean and std.dev: (4105.56, 2925.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (307, 361, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 667) [M::mem_pestat] mean and std.dev: (357.34, 80.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1808, 3933, 6515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15929) [M::mem_pestat] mean and std.dev: (4298.78, 2764.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20636) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1746, 3881, 6517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16059) [M::mem_pestat] mean and std.dev: (4233.02, 2810.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20830) [M::mem_process_seqs] Processed 1600000 reads in 463.475 CPU sec, 29.784 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12435, 156269, 11636, 12364) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1523, 3692, 6488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16418) [M::mem_pestat] mean and std.dev: (4098.20, 2906.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (307, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 667) [M::mem_pestat] mean and std.dev: (357.48, 81.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1787, 3990, 6597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16217) [M::mem_pestat] mean and std.dev: (4329.39, 2783.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21027) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1757, 3904, 6510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16016) [M::mem_pestat] mean and std.dev: (4257.63, 2822.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20769) [M::mem_process_seqs] Processed 1600000 reads in 463.185 CPU sec, 29.733 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13353, 155709, 11589, 12120) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1180, 3344, 6252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16396) [M::mem_pestat] mean and std.dev: (3819.17, 2948.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (357.65, 80.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1782, 3957, 6545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16071) [M::mem_pestat] mean and std.dev: (4303.21, 2789.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20834) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1746, 3923, 6486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15966) [M::mem_pestat] mean and std.dev: (4219.57, 2780.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20706) [M::mem_process_seqs] Processed 1600000 reads in 478.758 CPU sec, 30.631 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14622, 154327, 11707, 12135) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (632, 2891, 5978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16670) [M::mem_pestat] mean and std.dev: (3503.02, 3016.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22016) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.45, 80.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1813, 3949, 6604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16186) [M::mem_pestat] mean and std.dev: (4316.40, 2785.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20977) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1750, 3910, 6532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16096) [M::mem_pestat] mean and std.dev: (4255.28, 2804.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20878) [M::mem_process_seqs] Processed 1600000 reads in 509.119 CPU sec, 32.687 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14110, 154368, 11556, 12276) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (932, 3066, 6134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16538) [M::mem_pestat] mean and std.dev: (3644.51, 2983.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.77, 80.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1794, 3989, 6604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16224) [M::mem_pestat] mean and std.dev: (4326.86, 2788.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21034) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1741, 3946, 6581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16261) [M::mem_pestat] mean and std.dev: (4259.66, 2825.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21101) [M::mem_process_seqs] Processed 1600000 reads in 501.483 CPU sec, 32.180 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13568, 155231, 11768, 12184) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1222, 3403, 6224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16228) [M::mem_pestat] mean and std.dev: (3838.99, 2935.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21230) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.30, 80.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1777, 3965, 6556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16114) [M::mem_pestat] mean and std.dev: (4311.23, 2784.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20893) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1710, 3824, 6479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16017) [M::mem_pestat] mean and std.dev: (4195.43, 2794.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20786) [M::mem_process_seqs] Processed 1600000 reads in 487.549 CPU sec, 31.229 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13047, 155964, 11556, 12303) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1361, 3594, 6361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16361) [M::mem_pestat] mean and std.dev: (3960.73, 2917.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 362, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.44, 80.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1783, 3950, 6577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16165) [M::mem_pestat] mean and std.dev: (4311.90, 2797.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20959) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1721, 3899, 6498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16052) [M::mem_pestat] mean and std.dev: (4229.39, 2809.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20829) [M::mem_process_seqs] Processed 1600000 reads in 479.294 CPU sec, 30.653 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12791, 156319, 11585, 12059) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1455, 3649, 6413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16329) [M::mem_pestat] mean and std.dev: (4022.68, 2892.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21287) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.53, 80.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1811, 4002, 6583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16127) [M::mem_pestat] mean and std.dev: (4330.90, 2782.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20899) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1777, 3995, 6606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16264) [M::mem_pestat] mean and std.dev: (4291.25, 2820.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21093) [M::mem_process_seqs] Processed 1600000 reads in 470.960 CPU sec, 30.220 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12947, 155443, 11746, 12147) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1460, 3683, 6378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16214) [M::mem_pestat] mean and std.dev: (4035.41, 2894.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21132) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.11, 80.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1796, 3970, 6529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15995) [M::mem_pestat] mean and std.dev: (4301.12, 2769.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20728) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1758, 3967, 6524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16056) [M::mem_pestat] mean and std.dev: (4260.98, 2812.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20822) [M::mem_process_seqs] Processed 1600000 reads in 471.294 CPU sec, 30.180 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12985, 156209, 11530, 12273) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1464, 3745, 6477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16503) [M::mem_pestat] mean and std.dev: (4067.92, 2896.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (357.78, 80.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1823, 4035, 6601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16157) [M::mem_pestat] mean and std.dev: (4353.60, 2781.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20935) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1741, 3922, 6570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16228) [M::mem_pestat] mean and std.dev: (4270.54, 2830.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21057) [M::mem_process_seqs] Processed 1600000 reads in 465.771 CPU sec, 29.803 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12754, 156668, 11700, 12131) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1478, 3560, 6388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16208) [M::mem_pestat] mean and std.dev: (4004.21, 2868.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21118) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (357.62, 80.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1801, 3930, 6504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15910) [M::mem_pestat] mean and std.dev: (4290.44, 2752.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20613) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1760, 3892, 6499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15977) [M::mem_pestat] mean and std.dev: (4246.25, 2809.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20716) [M::mem_process_seqs] Processed 1600000 reads in 468.241 CPU sec, 30.035 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12810, 156734, 11582, 12146) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1459, 3648, 6403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16291) [M::mem_pestat] mean and std.dev: (4042.91, 2904.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21235) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (307, 361, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 667) [M::mem_pestat] mean and std.dev: (357.24, 80.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1804, 3988, 6567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16093) [M::mem_pestat] mean and std.dev: (4327.51, 2774.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20856) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1695, 3901, 6541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16233) [M::mem_pestat] mean and std.dev: (4236.54, 2830.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21079) [M::mem_process_seqs] Processed 1600000 reads in 466.736 CPU sec, 29.859 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12503, 156448, 11475, 12283) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1492, 3705, 6406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16234) [M::mem_pestat] mean and std.dev: (4062.93, 2873.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21148) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (307, 361, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 664) [M::mem_pestat] mean and std.dev: (357.14, 80.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1837, 3967, 6487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15787) [M::mem_pestat] mean and std.dev: (4304.80, 2747.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20437) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1772, 3971, 6655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16421) [M::mem_pestat] mean and std.dev: (4307.16, 2842.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21304) [M::mem_process_seqs] Processed 1600000 reads in 465.844 CPU sec, 29.878 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14950, 154751, 11688, 12129) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (675, 2825, 5945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16485) [M::mem_pestat] mean and std.dev: (3479.12, 2986.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (358.87, 81.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1792, 4026, 6548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16060) [M::mem_pestat] mean and std.dev: (4331.35, 2777.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20816) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1765, 3933, 6525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16045) [M::mem_pestat] mean and std.dev: (4264.39, 2809.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20805) [M::mem_process_seqs] Processed 1600000 reads in 498.420 CPU sec, 31.898 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14962, 153761, 11569, 12227) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (517, 2743, 5917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16717) [M::mem_pestat] mean and std.dev: (3437.29, 3016.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22117) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (358.93, 80.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1828, 4041, 6637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16255) [M::mem_pestat] mean and std.dev: (4373.83, 2786.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21064) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1789, 3970, 6562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16108) [M::mem_pestat] mean and std.dev: (4275.95, 2792.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20881) [M::mem_process_seqs] Processed 1600000 reads in 501.484 CPU sec, 32.165 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13833, 154711, 11667, 12351) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (997, 3141, 6079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16243) [M::mem_pestat] mean and std.dev: (3661.11, 2948.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.73, 80.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1782, 3936, 6525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16011) [M::mem_pestat] mean and std.dev: (4289.58, 2767.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20754) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1731, 3929, 6524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16110) [M::mem_pestat] mean and std.dev: (4241.44, 2806.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20903) [M::mem_process_seqs] Processed 1600000 reads in 494.469 CPU sec, 31.562 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13525, 155779, 11721, 12157) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1265, 3441, 6327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16451) [M::mem_pestat] mean and std.dev: (3888.38, 2945.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.67, 80.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1805, 3966, 6532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15986) [M::mem_pestat] mean and std.dev: (4306.17, 2762.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20713) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1713, 3907, 6489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16041) [M::mem_pestat] mean and std.dev: (4210.74, 2791.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20817) [M::mem_process_seqs] Processed 1600000 reads in 482.845 CPU sec, 30.879 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12917, 156594, 11587, 12185) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1373, 3588, 6373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16373) [M::mem_pestat] mean and std.dev: (3980.24, 2923.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (358.88, 81.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1808, 3991, 6517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15935) [M::mem_pestat] mean and std.dev: (4320.34, 2771.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20644) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1726, 3925, 6527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16129) [M::mem_pestat] mean and std.dev: (4251.00, 2813.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20930) [M::mem_process_seqs] Processed 1600000 reads in 468.291 CPU sec, 30.089 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12697, 156188, 11481, 12241) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1487, 3717, 6482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16472) [M::mem_pestat] mean and std.dev: (4074.52, 2908.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (66, 671) [M::mem_pestat] mean and std.dev: (358.76, 81.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1784, 3946, 6581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16175) [M::mem_pestat] mean and std.dev: (4311.40, 2791.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20972) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1743, 3938, 6565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16209) [M::mem_pestat] mean and std.dev: (4249.71, 2819.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21031) [M::mem_process_seqs] Processed 1600000 reads in 464.549 CPU sec, 29.755 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12889, 156841, 11644, 12276) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1492, 3620, 6360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16096) [M::mem_pestat] mean and std.dev: (4026.36, 2879.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.38, 81.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1802, 4016, 6523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15965) [M::mem_pestat] mean and std.dev: (4326.73, 2777.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20686) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1745, 3865, 6552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16166) [M::mem_pestat] mean and std.dev: (4233.79, 2816.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20973) [M::mem_process_seqs] Processed 1600000 reads in 466.052 CPU sec, 29.903 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12764, 156981, 11419, 12095) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1489, 3664, 6405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16237) [M::mem_pestat] mean and std.dev: (4045.58, 2890.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21153) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (358.19, 80.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1820, 3993, 6633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16259) [M::mem_pestat] mean and std.dev: (4339.84, 2798.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21072) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1719, 3907, 6528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16146) [M::mem_pestat] mean and std.dev: (4240.39, 2824.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20955) [M::mem_process_seqs] Processed 1600000 reads in 477.970 CPU sec, 30.617 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12967, 157146, 11375, 11959) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1469, 3660, 6433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16361) [M::mem_pestat] mean and std.dev: (4045.33, 2887.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (307, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 667) [M::mem_pestat] mean and std.dev: (357.48, 80.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1783, 4054, 6655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16399) [M::mem_pestat] mean and std.dev: (4354.68, 2795.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21271) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1737, 3888, 6553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16185) [M::mem_pestat] mean and std.dev: (4233.30, 2810.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21001) [M::mem_process_seqs] Processed 1600000 reads in 463.626 CPU sec, 29.836 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12583, 157272, 11669, 12222) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1515, 3755, 6518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16524) [M::mem_pestat] mean and std.dev: (4119.64, 2902.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (307, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 667) [M::mem_pestat] mean and std.dev: (357.63, 80.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1799, 3985, 6583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16151) [M::mem_pestat] mean and std.dev: (4332.48, 2789.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20935) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1717, 3850, 6497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16057) [M::mem_pestat] mean and std.dev: (4210.07, 2810.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20837) [M::mem_process_seqs] Processed 1600000 reads in 466.209 CPU sec, 29.777 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13601, 155890, 11571, 12222) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1162, 3313, 6204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16288) [M::mem_pestat] mean and std.dev: (3792.56, 2945.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 361, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (357.21, 80.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1815, 3944, 6529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15957) [M::mem_pestat] mean and std.dev: (4310.57, 2779.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20671) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1735, 3843, 6448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15874) [M::mem_pestat] mean and std.dev: (4203.17, 2786.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20587) [M::mem_process_seqs] Processed 1600000 reads in 484.059 CPU sec, 30.972 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15735, 152339, 11511, 12098) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 2471, 5719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16553) [M::mem_pestat] mean and std.dev: (3277.94, 3006.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21970) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.68, 80.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1804, 3988, 6627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16273) [M::mem_pestat] mean and std.dev: (4340.66, 2782.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21096) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1765, 3990, 6604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16282) [M::mem_pestat] mean and std.dev: (4299.71, 2826.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21121) [M::mem_process_seqs] Processed 1600000 reads in 531.105 CPU sec, 34.337 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14931, 152860, 11652, 12314) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (606, 2789, 6023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16857) [M::mem_pestat] mean and std.dev: (3478.18, 3035.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (359.16, 80.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1817, 4025, 6571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16079) [M::mem_pestat] mean and std.dev: (4343.52, 2776.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20833) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1750, 3956, 6456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15868) [M::mem_pestat] mean and std.dev: (4238.29, 2793.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20574) [M::mem_process_seqs] Processed 1600000 reads in 522.874 CPU sec, 33.393 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13791, 154592, 11617, 12046) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1001, 3222, 6162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16484) [M::mem_pestat] mean and std.dev: (3712.97, 2975.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 364, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (359.05, 80.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1792, 3965, 6549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16063) [M::mem_pestat] mean and std.dev: (4324.43, 2782.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20820) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1761, 3989, 6577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16209) [M::mem_pestat] mean and std.dev: (4279.57, 2821.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21025) [M::mem_process_seqs] Processed 1600000 reads in 506.114 CPU sec, 32.571 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13362, 155514, 11590, 12295) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1228, 3387, 6270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16354) [M::mem_pestat] mean and std.dev: (3858.42, 2949.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.65, 80.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1772, 3982, 6553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16115) [M::mem_pestat] mean and std.dev: (4305.87, 2789.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20896) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1717, 3860, 6400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15766) [M::mem_pestat] mean and std.dev: (4174.68, 2773.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20449) [M::mem_process_seqs] Processed 1600000 reads in 494.971 CPU sec, 31.550 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13022, 155126, 11797, 12165) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1383, 3562, 6355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16299) [M::mem_pestat] mean and std.dev: (3967.01, 2911.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21271) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (359.04, 80.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1805, 3965, 6545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16025) [M::mem_pestat] mean and std.dev: (4320.36, 2793.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20765) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1748, 3935, 6555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16169) [M::mem_pestat] mean and std.dev: (4258.23, 2803.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20976) [M::mem_process_seqs] Processed 1600000 reads in 482.923 CPU sec, 30.825 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13024, 155923, 11673, 12184) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1420, 3636, 6395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16345) [M::mem_pestat] mean and std.dev: (4009.33, 2906.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.42, 80.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1826, 4022, 6612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16184) [M::mem_pestat] mean and std.dev: (4341.48, 2760.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20970) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1747, 3872, 6452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15862) [M::mem_pestat] mean and std.dev: (4214.03, 2793.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20567) [M::mem_process_seqs] Processed 1600000 reads in 479.808 CPU sec, 30.663 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12910, 155687, 11598, 12215) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1467, 3636, 6388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16230) [M::mem_pestat] mean and std.dev: (4029.24, 2887.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21151) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.60, 81.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1785, 3966, 6524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16002) [M::mem_pestat] mean and std.dev: (4311.38, 2777.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20741) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1757, 3867, 6522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16052) [M::mem_pestat] mean and std.dev: (4232.82, 2809.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20817) [M::mem_process_seqs] Processed 1600000 reads in 477.868 CPU sec, 30.522 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13001, 155894, 11566, 12267) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1448, 3596, 6331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16097) [M::mem_pestat] mean and std.dev: (3990.24, 2870.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.21, 81.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1802, 4007, 6567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16097) [M::mem_pestat] mean and std.dev: (4326.31, 2776.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1791, 3985, 6565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16113) [M::mem_pestat] mean and std.dev: (4290.31, 2818.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20887) [M::mem_process_seqs] Processed 1600000 reads in 471.374 CPU sec, 30.127 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12899, 156500, 11588, 11997) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1458, 3641, 6380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16224) [M::mem_pestat] mean and std.dev: (4029.02, 2881.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21146) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (357.93, 80.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1790, 3983, 6597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16211) [M::mem_pestat] mean and std.dev: (4322.37, 2787.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21018) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1716, 3912, 6506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16086) [M::mem_pestat] mean and std.dev: (4234.19, 2814.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20876) [M::mem_process_seqs] Processed 1600000 reads in 473.521 CPU sec, 30.242 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13033, 156149, 11519, 11973) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1326, 3438, 6323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16317) [M::mem_pestat] mean and std.dev: (3910.81, 2934.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (307, 361, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 664) [M::mem_pestat] mean and std.dev: (357.25, 80.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1820, 3963, 6450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15710) [M::mem_pestat] mean and std.dev: (4300.68, 2749.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20340) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1785, 3883, 6536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16038) [M::mem_pestat] mean and std.dev: (4242.47, 2806.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20789) [M::mem_process_seqs] Processed 1600000 reads in 481.611 CPU sec, 30.810 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15591, 153699, 11525, 12255) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 2539, 5924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17144) [M::mem_pestat] mean and std.dev: (3344.64, 3060.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 362, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.64, 80.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1784, 4002, 6592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16208) [M::mem_pestat] mean and std.dev: (4333.63, 2778.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21016) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1750, 3947, 6524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16072) [M::mem_pestat] mean and std.dev: (4262.53, 2807.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20846) [M::mem_process_seqs] Processed 1600000 reads in 548.081 CPU sec, 35.169 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13774, 155147, 11738, 12183) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1061, 3206, 6185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16433) [M::mem_pestat] mean and std.dev: (3726.19, 2968.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.81, 80.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1823, 4019, 6575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16079) [M::mem_pestat] mean and std.dev: (4340.09, 2770.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20831) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1762, 3916, 6542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16102) [M::mem_pestat] mean and std.dev: (4260.70, 2791.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20882) [M::mem_process_seqs] Processed 1600000 reads in 504.063 CPU sec, 32.231 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13140, 156296, 11563, 12266) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1402, 3627, 6418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16450) [M::mem_pestat] mean and std.dev: (4012.73, 2920.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (358.83, 80.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1747, 3912, 6527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16087) [M::mem_pestat] mean and std.dev: (4278.18, 2788.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20867) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1758, 3911, 6538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16098) [M::mem_pestat] mean and std.dev: (4253.55, 2819.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20878) [M::mem_process_seqs] Processed 1600000 reads in 477.334 CPU sec, 30.528 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12928, 155892, 11496, 12250) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1445, 3685, 6436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16418) [M::mem_pestat] mean and std.dev: (4040.37, 2898.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (358.25, 80.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1827, 4024, 6587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16107) [M::mem_pestat] mean and std.dev: (4351.31, 2780.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20867) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1785, 3940, 6507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15951) [M::mem_pestat] mean and std.dev: (4256.82, 2797.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20673) [M::mem_process_seqs] Processed 1600000 reads in 482.560 CPU sec, 30.882 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12777, 155537, 11547, 12104) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1478, 3587, 6345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16079) [M::mem_pestat] mean and std.dev: (4016.54, 2875.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20946) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (358.21, 80.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1781, 3949, 6579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16175) [M::mem_pestat] mean and std.dev: (4301.26, 2790.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20973) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1742, 3888, 6459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15893) [M::mem_pestat] mean and std.dev: (4214.00, 2799.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20610) [M::mem_process_seqs] Processed 1600000 reads in 481.790 CPU sec, 30.877 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12771, 155960, 11664, 12174) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1440, 3693, 6394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16302) [M::mem_pestat] mean and std.dev: (4031.47, 2890.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21256) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (307, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 667) [M::mem_pestat] mean and std.dev: (357.82, 80.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1818, 3947, 6542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15990) [M::mem_pestat] mean and std.dev: (4320.38, 2775.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20714) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1774, 3977, 6579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16189) [M::mem_pestat] mean and std.dev: (4281.39, 2819.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20994) [M::mem_process_seqs] Processed 1600000 reads in 475.084 CPU sec, 30.405 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12877, 155648, 11580, 12323) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1448, 3695, 6451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16457) [M::mem_pestat] mean and std.dev: (4056.55, 2901.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (307, 361, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 664) [M::mem_pestat] mean and std.dev: (357.12, 80.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1800, 4030, 6582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16146) [M::mem_pestat] mean and std.dev: (4330.25, 2760.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20928) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1723, 3918, 6485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16009) [M::mem_pestat] mean and std.dev: (4221.37, 2804.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20771) [M::mem_process_seqs] Processed 1600000 reads in 492.310 CPU sec, 31.558 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14138, 155348, 11686, 12212) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (887, 3110, 6117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16577) [M::mem_pestat] mean and std.dev: (3642.80, 3001.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 665) [M::mem_pestat] mean and std.dev: (357.79, 80.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1805, 4030, 6547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16031) [M::mem_pestat] mean and std.dev: (4342.69, 2783.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20773) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1758, 4016, 6568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16188) [M::mem_pestat] mean and std.dev: (4279.27, 2816.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20998) [M::mem_process_seqs] Processed 1600000 reads in 502.185 CPU sec, 32.279 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16453, 152978, 11554, 12144) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 2276, 5581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16483) [M::mem_pestat] mean and std.dev: (3144.24, 3024.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21934) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 364, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (359.28, 80.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1800, 4000, 6588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16164) [M::mem_pestat] mean and std.dev: (4329.16, 2783.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20952) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1801, 3950, 6557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16069) [M::mem_pestat] mean and std.dev: (4269.37, 2802.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20825) [M::mem_process_seqs] Processed 1600000 reads in 521.693 CPU sec, 33.581 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15162, 152425, 11721, 12294) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (434, 2679, 5904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16844) [M::mem_pestat] mean and std.dev: (3388.24, 3016.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 364, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (359.02, 81.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1792, 3995, 6554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16078) [M::mem_pestat] mean and std.dev: (4318.16, 2767.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20840) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1750, 3881, 6441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15823) [M::mem_pestat] mean and std.dev: (4212.21, 2780.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20514) [M::mem_process_seqs] Processed 1600000 reads in 522.269 CPU sec, 33.407 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14349, 155035, 11416, 12173) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (864, 3026, 6113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16611) [M::mem_pestat] mean and std.dev: (3608.45, 2994.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.87, 81.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1805, 3943, 6518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15944) [M::mem_pestat] mean and std.dev: (4294.45, 2767.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20657) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1745, 3955, 6493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15989) [M::mem_pestat] mean and std.dev: (4242.19, 2796.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20737) [M::mem_process_seqs] Processed 1600000 reads in 507.442 CPU sec, 32.670 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13422, 155165, 11394, 12272) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1181, 3355, 6231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16331) [M::mem_pestat] mean and std.dev: (3820.44, 2952.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (359.23, 81.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1799, 4016, 6692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16478) [M::mem_pestat] mean and std.dev: (4365.19, 2802.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21371) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1770, 3950, 6535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16065) [M::mem_pestat] mean and std.dev: (4271.41, 2815.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20830) [M::mem_process_seqs] Processed 1600000 reads in 493.360 CPU sec, 31.596 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13069, 155868, 11544, 12159) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1402, 3544, 6313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16135) [M::mem_pestat] mean and std.dev: (3965.05, 2909.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 669) [M::mem_pestat] mean and std.dev: (359.12, 81.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1783, 3980, 6627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16315) [M::mem_pestat] mean and std.dev: (4338.79, 2801.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21159) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1697, 3887, 6526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16184) [M::mem_pestat] mean and std.dev: (4218.20, 2826.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21013) [M::mem_process_seqs] Processed 1600000 reads in 479.048 CPU sec, 30.756 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12816, 156414, 11659, 12126) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1384, 3636, 6428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16516) [M::mem_pestat] mean and std.dev: (4003.84, 2924.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (309, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 666) [M::mem_pestat] mean and std.dev: (358.79, 81.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1813, 4014, 6653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16333) [M::mem_pestat] mean and std.dev: (4352.34, 2797.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21173) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1715, 3886, 6466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15968) [M::mem_pestat] mean and std.dev: (4210.99, 2798.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20719) [M::mem_process_seqs] Processed 1600000 reads in 473.667 CPU sec, 30.352 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12933, 156260, 11529, 12134) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1454, 3699, 6421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16355) [M::mem_pestat] mean and std.dev: (4052.45, 2905.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21322) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (66, 671) [M::mem_pestat] mean and std.dev: (358.47, 81.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1788, 3969, 6531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16017) [M::mem_pestat] mean and std.dev: (4315.17, 2776.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20760) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1729, 3914, 6548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16186) [M::mem_pestat] mean and std.dev: (4243.92, 2824.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21005) [M::mem_process_seqs] Processed 1600000 reads in 474.091 CPU sec, 30.338 real sec [M::process] read 1600000 sequences (200000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12776, 156235, 11437, 11898) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1533, 3772, 6469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16341) [M::mem_pestat] mean and std.dev: (4100.71, 2880.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21277) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 363, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 668) [M::mem_pestat] mean and std.dev: (358.21, 80.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1816, 4010, 6629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16255) [M::mem_pestat] mean and std.dev: (4352.24, 2784.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21068) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1709, 3942, 6507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16103) [M::mem_pestat] mean and std.dev: (4236.80, 2809.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20901) [M::mem_process_seqs] Processed 1600000 reads in 479.743 CPU sec, 31.142 real sec [M::process] read 1018014 sequences (127251750 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12766, 156285, 11464, 12267) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1491, 3693, 6381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16161) [M::mem_pestat] mean and std.dev: (4049.24, 2871.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21051) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (307, 362, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 667) [M::mem_pestat] mean and std.dev: (357.49, 80.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1798, 3963, 6533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16003) [M::mem_pestat] mean and std.dev: (4305.37, 2778.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20738) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1762, 3888, 6551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16129) [M::mem_pestat] mean and std.dev: (4240.27, 2823.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20918) [M::mem_process_seqs] Processed 1600000 reads in 486.224 CPU sec, 31.374 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8067, 100330, 7370, 7683) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1494, 3735, 6431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16305) [M::mem_pestat] mean and std.dev: (4082.46, 2880.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21242) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (308, 362, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (72, 662) [M::mem_pestat] mean and std.dev: (357.87, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1784, 4000, 6630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16322) [M::mem_pestat] mean and std.dev: (4340.65, 2804.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21168) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1706, 3950, 6571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16301) [M::mem_pestat] mean and std.dev: (4255.81, 2835.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21166) [M::mem_process_seqs] Processed 1018014 reads in 310.239 CPU sec, 20.312 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -SP5M -t 20 /oak/stanford/groups/akundaje/marinovg/genomes/Ciliates/Tetrahymena_thermophila_SB210-E-JCVI_TeTherMIC_3/bwa-indexes/Tetrahymena_thermophila_SB210-E-JCVI_TeTherMIC_3.fa /oak/stanford/groups/akundaje/marinovg/papers/2022_Tetrahymena-Hi-C/2022-05-24-figures/juicer-MIC_24_hpm_Hi-C-GSM4161210-MIC/splits/Reads_R1.fastq.gz /oak/stanford/groups/akundaje/marinovg/papers/2022_Tetrahymena-Hi-C/2022-05-24-figures/juicer-MIC_24_hpm_Hi-C-GSM4161210-MIC/splits/Reads_R2.fastq.gz [main] Real time: 3895.183 sec; CPU: 59899.661 sec Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m