[M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3831, 397039, 3409, 3688) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1349, 3315, 5990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15272) [M::mem_pestat] mean and std.dev: (3825.25, 2827.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19913) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 186, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (199.26, 78.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1735, 3797, 6322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15496) [M::mem_pestat] mean and std.dev: (4181.73, 2768.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20083) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1620, 3607, 6372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15876) [M::mem_pestat] mean and std.dev: (4107.13, 2824.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20628) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 357.028 CPU sec, 48.321 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3764, 391474, 3310, 3577) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1411, 3322, 6088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15442) [M::mem_pestat] mean and std.dev: (3898.15, 2851.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20119) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 186, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (199.05, 77.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1540, 3498, 6265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15715) [M::mem_pestat] mean and std.dev: (3999.90, 2795.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20440) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1654, 3615, 6315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15637) [M::mem_pestat] mean and std.dev: (4110.09, 2784.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20298) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 323.235 CPU sec, 21.100 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3639, 393072, 3385, 3568) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1337, 3314, 5991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15299) [M::mem_pestat] mean and std.dev: (3834.06, 2841.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 186, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (198.70, 77.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1586, 3676, 6267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15629) [M::mem_pestat] mean and std.dev: (4112.27, 2853.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20310) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1612, 3570, 6110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15106) [M::mem_pestat] mean and std.dev: (4032.42, 2764.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19604) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 318.515 CPU sec, 20.326 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3775, 392390, 3471, 3610) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1377, 3390, 6129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15633) [M::mem_pestat] mean and std.dev: (3889.95, 2840.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 187, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (199.53, 78.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1591, 3514, 6184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15370) [M::mem_pestat] mean and std.dev: (4004.55, 2758.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19963) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1638, 3625, 6256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15492) [M::mem_pestat] mean and std.dev: (4051.11, 2791.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20110) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 314.877 CPU sec, 20.074 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3789, 392177, 3561, 3548) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1357, 3224, 5918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15040) [M::mem_pestat] mean and std.dev: (3795.54, 2817.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 187, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (199.79, 78.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1538, 3566, 6289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15791) [M::mem_pestat] mean and std.dev: (4052.23, 2824.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20542) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1572, 3675, 6375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15981) [M::mem_pestat] mean and std.dev: (4091.29, 2811.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20784) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 318.255 CPU sec, 20.490 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3776, 394298, 3503, 3531) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1413, 3346, 6191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15747) [M::mem_pestat] mean and std.dev: (3906.66, 2872.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 187, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (200.30, 79.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1536, 3476, 6243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15657) [M::mem_pestat] mean and std.dev: (4010.95, 2815.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20364) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1541, 3528, 6148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15362) [M::mem_pestat] mean and std.dev: (3966.47, 2747.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19969) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 309.868 CPU sec, 19.741 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3735, 389500, 3443, 3597) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1409, 3447, 6178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15716) [M::mem_pestat] mean and std.dev: (3926.00, 2839.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 187, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (200.37, 79.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1662, 3628, 6138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15090) [M::mem_pestat] mean and std.dev: (4045.16, 2758.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19566) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1556, 3525, 6291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15761) [M::mem_pestat] mean and std.dev: (4044.77, 2805.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20496) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 317.863 CPU sec, 20.294 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3794, 390123, 3593, 3744) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1318, 3328, 6039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15481) [M::mem_pestat] mean and std.dev: (3819.26, 2819.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 188, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (200.72, 79.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1583, 3547, 6214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15476) [M::mem_pestat] mean and std.dev: (4065.77, 2789.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20107) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1594, 3496, 6062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14998) [M::mem_pestat] mean and std.dev: (4005.52, 2777.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19466) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 312.896 CPU sec, 19.952 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3613, 392438, 3372, 3532) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1377, 3389, 6015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15291) [M::mem_pestat] mean and std.dev: (3851.66, 2829.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19929) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 186, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (198.32, 77.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1613, 3741, 6247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15515) [M::mem_pestat] mean and std.dev: (4086.55, 2790.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20149) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1608, 3525, 6263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15573) [M::mem_pestat] mean and std.dev: (4054.42, 2793.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20228) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 329.180 CPU sec, 21.001 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3678, 385440, 3427, 3523) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1326, 3338, 5955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15213) [M::mem_pestat] mean and std.dev: (3826.40, 2812.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (197.68, 77.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1527, 3496, 6202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15552) [M::mem_pestat] mean and std.dev: (3997.79, 2816.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20227) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1551, 3428, 6036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15006) [M::mem_pestat] mean and std.dev: (3919.22, 2738.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19491) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 332.300 CPU sec, 21.226 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3604, 382826, 3373, 3450) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1328, 3198, 5807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14765) [M::mem_pestat] mean and std.dev: (3742.31, 2810.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19244) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (197.64, 77.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1574, 3533, 6163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15341) [M::mem_pestat] mean and std.dev: (4029.72, 2805.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19930) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1561, 3544, 6089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15145) [M::mem_pestat] mean and std.dev: (3999.18, 2799.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19673) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 332.796 CPU sec, 21.236 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3771, 384217, 3240, 3450) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1330, 3307, 6064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15532) [M::mem_pestat] mean and std.dev: (3836.62, 2851.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20266) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 185, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (198.10, 77.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1544, 3607, 6349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15959) [M::mem_pestat] mean and std.dev: (4107.80, 2857.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20764) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1560, 3607, 6101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15183) [M::mem_pestat] mean and std.dev: (4006.13, 2776.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19724) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 330.783 CPU sec, 21.074 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3754, 387412, 3437, 3594) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1357, 3356, 5989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15253) [M::mem_pestat] mean and std.dev: (3839.03, 2844.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 187, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (199.93, 78.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1649, 3742, 6372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15818) [M::mem_pestat] mean and std.dev: (4171.19, 2828.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20541) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1693, 3775, 6474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16036) [M::mem_pestat] mean and std.dev: (4199.80, 2820.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20817) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 319.855 CPU sec, 20.369 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3782, 387256, 3469, 3681) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1270, 3306, 5874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15082) [M::mem_pestat] mean and std.dev: (3765.43, 2808.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 188, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (200.80, 79.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1570, 3606, 6208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15484) [M::mem_pestat] mean and std.dev: (4052.00, 2797.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20122) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1553, 3476, 6193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15473) [M::mem_pestat] mean and std.dev: (3988.36, 2789.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20113) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 317.739 CPU sec, 20.299 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3785, 382616, 3319, 3537) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1348, 3454, 6001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15307) [M::mem_pestat] mean and std.dev: (3869.82, 2822.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19960) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 188, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (200.82, 79.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1680, 3723, 6354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15702) [M::mem_pestat] mean and std.dev: (4147.01, 2799.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20376) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1572, 3617, 6086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15114) [M::mem_pestat] mean and std.dev: (4017.86, 2767.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19628) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 319.380 CPU sec, 20.278 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3783, 383222, 3418, 3650) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1294, 3262, 5840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14932) [M::mem_pestat] mean and std.dev: (3763.02, 2822.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 187, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (200.27, 79.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1589, 3571, 6224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15494) [M::mem_pestat] mean and std.dev: (4033.98, 2775.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20129) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1582, 3420, 6099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15133) [M::mem_pestat] mean and std.dev: (3987.72, 2771.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19650) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 326.940 CPU sec, 20.799 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3989, 388947, 3559, 3712) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1274, 3260, 6013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15491) [M::mem_pestat] mean and std.dev: (3802.19, 2860.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20230) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 187, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (199.73, 77.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1598, 3639, 6241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15527) [M::mem_pestat] mean and std.dev: (4067.64, 2817.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20170) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1587, 3626, 6254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15588) [M::mem_pestat] mean and std.dev: (4058.04, 2808.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20255) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 340.947 CPU sec, 21.776 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3941, 386237, 3605, 3674) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1307, 3329, 5986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15344) [M::mem_pestat] mean and std.dev: (3804.59, 2830.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20023) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 187, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (199.86, 78.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1652, 3606, 6189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15263) [M::mem_pestat] mean and std.dev: (4035.57, 2738.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19800) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1614, 3687, 6433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16071) [M::mem_pestat] mean and std.dev: (4125.56, 2814.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20890) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 339.883 CPU sec, 21.663 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3935, 387276, 3585, 3751) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1324, 3320, 5986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15310) [M::mem_pestat] mean and std.dev: (3802.34, 2819.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 188, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (200.42, 78.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1568, 3642, 6185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15419) [M::mem_pestat] mean and std.dev: (4045.97, 2790.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20036) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1474, 3499, 6114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15394) [M::mem_pestat] mean and std.dev: (3955.94, 2762.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20034) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 333.166 CPU sec, 21.249 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3937, 385567, 3543, 3693) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1415, 3378, 6228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15854) [M::mem_pestat] mean and std.dev: (3918.78, 2861.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 188, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (200.79, 79.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1570, 3404, 6133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15259) [M::mem_pestat] mean and std.dev: (3960.40, 2770.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19822) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1496, 3595, 6256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15776) [M::mem_pestat] mean and std.dev: (4050.02, 2831.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20536) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 328.264 CPU sec, 20.919 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4064, 389121, 3604, 3798) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1242, 3231, 5999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15513) [M::mem_pestat] mean and std.dev: (3786.69, 2884.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 189, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (201.61, 79.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1579, 3521, 6172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15358) [M::mem_pestat] mean and std.dev: (3989.10, 2776.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19951) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1555, 3448, 6095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15175) [M::mem_pestat] mean and std.dev: (3985.51, 2782.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19715) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 318.058 CPU sec, 20.500 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3950, 385593, 3661, 3807) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1262, 3308, 5891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15149) [M::mem_pestat] mean and std.dev: (3756.42, 2795.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 189, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (201.93, 79.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1581, 3573, 6262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15624) [M::mem_pestat] mean and std.dev: (4058.64, 2782.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20305) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1531, 3444, 6084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15190) [M::mem_pestat] mean and std.dev: (3978.83, 2769.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19743) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 322.357 CPU sec, 20.554 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3986, 386185, 3579, 3778) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1309, 3355, 6044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15514) [M::mem_pestat] mean and std.dev: (3836.08, 2842.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20249) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 190, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (202.80, 80.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1732, 3679, 6318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15490) [M::mem_pestat] mean and std.dev: (4139.79, 2790.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20076) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1598, 3545, 6170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15314) [M::mem_pestat] mean and std.dev: (4011.19, 2768.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19886) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 316.332 CPU sec, 20.321 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3869, 391704, 3511, 3654) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1318, 3237, 6047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15505) [M::mem_pestat] mean and std.dev: (3805.17, 2852.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20234) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 188, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (200.77, 79.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1724, 3642, 6259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15329) [M::mem_pestat] mean and std.dev: (4128.67, 2790.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19864) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1580, 3521, 6163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15329) [M::mem_pestat] mean and std.dev: (4013.69, 2775.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19912) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 326.956 CPU sec, 20.954 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3948, 383781, 3489, 3736) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1247, 3235, 5964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15398) [M::mem_pestat] mean and std.dev: (3772.09, 2842.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20115) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 187, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (198.98, 77.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1623, 3639, 6172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15270) [M::mem_pestat] mean and std.dev: (4073.83, 2802.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19819) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1651, 3488, 6114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15040) [M::mem_pestat] mean and std.dev: (4009.89, 2741.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19503) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 342.268 CPU sec, 21.674 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3961, 388627, 3535, 3672) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1198, 3152, 5980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15544) [M::mem_pestat] mean and std.dev: (3754.19, 2882.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20326) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 188, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (200.30, 78.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1572, 3516, 6197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15447) [M::mem_pestat] mean and std.dev: (3996.56, 2786.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20072) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1536, 3492, 6091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15201) [M::mem_pestat] mean and std.dev: (3976.55, 2766.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19756) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 328.771 CPU sec, 21.138 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3821, 390156, 3419, 3595) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1294, 3273, 5986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15370) [M::mem_pestat] mean and std.dev: (3792.65, 2831.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 188, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (201.05, 79.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1638, 3637, 6337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15735) [M::mem_pestat] mean and std.dev: (4123.33, 2823.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20434) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1597, 3572, 6191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15379) [M::mem_pestat] mean and std.dev: (4036.11, 2782.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19973) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 324.837 CPU sec, 20.625 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3933, 386663, 3580, 3826) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1358, 3320, 5987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15245) [M::mem_pestat] mean and std.dev: (3839.22, 2847.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 189, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (201.37, 79.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1536, 3536, 6222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15594) [M::mem_pestat] mean and std.dev: (4016.27, 2815.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20280) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1620, 3566, 6082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15006) [M::mem_pestat] mean and std.dev: (4004.80, 2726.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19468) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 329.455 CPU sec, 20.931 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3854, 387756, 3649, 3794) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1396, 3380, 5934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15010) [M::mem_pestat] mean and std.dev: (3861.27, 2834.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 189, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (201.87, 79.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1595, 3638, 6318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15764) [M::mem_pestat] mean and std.dev: (4087.30, 2813.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20487) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1573, 3549, 6173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15373) [M::mem_pestat] mean and std.dev: (4019.79, 2803.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19973) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 318.183 CPU sec, 20.403 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3909, 386544, 3666, 3777) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1234, 3150, 5845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15067) [M::mem_pestat] mean and std.dev: (3732.76, 2831.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 190, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (202.88, 80.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1527, 3531, 6333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15945) [M::mem_pestat] mean and std.dev: (4042.67, 2843.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20751) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1627, 3674, 6316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15694) [M::mem_pestat] mean and std.dev: (4102.76, 2786.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20383) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 321.360 CPU sec, 20.502 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3882, 388783, 3626, 3794) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1336, 3360, 6019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15385) [M::mem_pestat] mean and std.dev: (3864.02, 2847.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20068) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 192, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (205.31, 82.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1650, 3581, 6293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15579) [M::mem_pestat] mean and std.dev: (4102.47, 2823.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20222) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1578, 3444, 5978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14778) [M::mem_pestat] mean and std.dev: (3960.14, 2750.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19178) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 312.829 CPU sec, 20.227 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4053, 387112, 3619, 3861) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1346, 3287, 6022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15374) [M::mem_pestat] mean and std.dev: (3838.89, 2833.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20050) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 197, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (211.08, 86.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1667, 3705, 6340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15686) [M::mem_pestat] mean and std.dev: (4126.09, 2809.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20359) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1542, 3485, 6099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15213) [M::mem_pestat] mean and std.dev: (3983.94, 2765.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19770) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 307.320 CPU sec, 19.527 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4091, 386085, 3696, 3925) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1399, 3363, 6099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15499) [M::mem_pestat] mean and std.dev: (3888.14, 2821.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20199) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 197, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (211.00, 87.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1605, 3643, 6291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15663) [M::mem_pestat] mean and std.dev: (4082.47, 2816.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20349) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1723, 3723, 6242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15280) [M::mem_pestat] mean and std.dev: (4107.23, 2739.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19799) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 304.703 CPU sec, 19.600 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3928, 387561, 3654, 3958) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1436, 3376, 6183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15677) [M::mem_pestat] mean and std.dev: (3933.73, 2875.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 197, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (210.59, 86.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1725, 3753, 6332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15546) [M::mem_pestat] mean and std.dev: (4151.83, 2787.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20153) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1563, 3569, 6164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15366) [M::mem_pestat] mean and std.dev: (4018.95, 2793.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19967) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 304.590 CPU sec, 19.388 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4051, 387157, 3679, 4049) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1448, 3356, 5868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14708) [M::mem_pestat] mean and std.dev: (3838.22, 2778.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 197, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (210.58, 86.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1737, 3646, 6280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15366) [M::mem_pestat] mean and std.dev: (4106.47, 2761.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19909) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1536, 3509, 6119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15285) [M::mem_pestat] mean and std.dev: (3980.76, 2770.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19868) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 305.806 CPU sec, 19.499 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3882, 387997, 3822, 3793) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1473, 3323, 5982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15000) [M::mem_pestat] mean and std.dev: (3871.96, 2812.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 197, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (210.32, 86.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1618, 3615, 6171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15277) [M::mem_pestat] mean and std.dev: (4058.15, 2765.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19830) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1560, 3583, 6224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15552) [M::mem_pestat] mean and std.dev: (4034.12, 2786.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20216) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 304.077 CPU sec, 19.430 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4010, 386759, 3698, 3937) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1283, 3230, 5917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15185) [M::mem_pestat] mean and std.dev: (3774.74, 2824.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (209.11, 85.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1660, 3596, 6186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15238) [M::mem_pestat] mean and std.dev: (4060.76, 2807.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19764) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1576, 3584, 6226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15526) [M::mem_pestat] mean and std.dev: (4036.82, 2787.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20176) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 314.574 CPU sec, 20.078 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3992, 388430, 3779, 3892) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1325, 3180, 5845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14885) [M::mem_pestat] mean and std.dev: (3781.59, 2847.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (209.48, 86.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1548, 3356, 6176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15432) [M::mem_pestat] mean and std.dev: (3943.48, 2776.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20060) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1571, 3517, 6066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15056) [M::mem_pestat] mean and std.dev: (3965.89, 2734.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19551) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 301.771 CPU sec, 19.268 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3934, 388240, 3712, 3769) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1273, 3287, 5948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15298) [M::mem_pestat] mean and std.dev: (3794.40, 2820.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19973) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (209.37, 86.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1607, 3713, 6353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15845) [M::mem_pestat] mean and std.dev: (4121.65, 2806.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20591) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1545, 3457, 6141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15333) [M::mem_pestat] mean and std.dev: (3995.23, 2786.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19929) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 304.343 CPU sec, 19.516 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3948, 389205, 3786, 3893) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1327, 3268, 5927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15127) [M::mem_pestat] mean and std.dev: (3793.05, 2813.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (208.74, 85.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1592, 3569, 6154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15278) [M::mem_pestat] mean and std.dev: (4047.43, 2777.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19840) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1612, 3627, 6332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15772) [M::mem_pestat] mean and std.dev: (4115.48, 2798.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20492) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 300.359 CPU sec, 19.098 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4038, 389501, 3756, 3868) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1339, 3314, 5866) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14920) [M::mem_pestat] mean and std.dev: (3780.23, 2777.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 194, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (207.67, 85.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1629, 3708, 6385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15897) [M::mem_pestat] mean and std.dev: (4148.55, 2818.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1513, 3495, 6294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15856) [M::mem_pestat] mean and std.dev: (4014.42, 2819.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20637) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 306.293 CPU sec, 19.607 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4003, 392549, 3721, 3828) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1399, 3322, 6062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15388) [M::mem_pestat] mean and std.dev: (3871.06, 2811.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20051) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 193, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (207.35, 85.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1596, 3521, 6254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15570) [M::mem_pestat] mean and std.dev: (4036.00, 2800.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20228) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1567, 3594, 6202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15472) [M::mem_pestat] mean and std.dev: (4046.54, 2788.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20107) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 297.421 CPU sec, 18.993 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4077, 390393, 3707, 3952) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1284, 3253, 5870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15042) [M::mem_pestat] mean and std.dev: (3751.48, 2793.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 194, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (208.16, 85.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1591, 3665, 6331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15811) [M::mem_pestat] mean and std.dev: (4093.40, 2825.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20551) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1582, 3570, 6256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15604) [M::mem_pestat] mean and std.dev: (4055.41, 2809.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20278) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 309.140 CPU sec, 19.827 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3947, 387106, 3733, 3970) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1379, 3276, 5916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14990) [M::mem_pestat] mean and std.dev: (3808.65, 2819.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 198, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (211.34, 86.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1555, 3621, 6266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15688) [M::mem_pestat] mean and std.dev: (4046.24, 2822.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20399) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1588, 3570, 6077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15055) [M::mem_pestat] mean and std.dev: (4000.69, 2757.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19544) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 296.803 CPU sec, 18.867 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4044, 386958, 3600, 3871) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1351, 3167, 5843) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14827) [M::mem_pestat] mean and std.dev: (3750.18, 2782.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 197, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (210.67, 86.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1643, 3525, 6223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15383) [M::mem_pestat] mean and std.dev: (4061.67, 2795.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19963) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1649, 3518, 6039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14819) [M::mem_pestat] mean and std.dev: (4014.75, 2767.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19209) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 308.794 CPU sec, 19.876 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4052, 387099, 3734, 3959) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1403, 3417, 6063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15383) [M::mem_pestat] mean and std.dev: (3897.80, 2824.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20043) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 197, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (211.23, 86.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1664, 3528, 6152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15128) [M::mem_pestat] mean and std.dev: (4036.47, 2765.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19616) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1623, 3621, 6152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15210) [M::mem_pestat] mean and std.dev: (4053.01, 2784.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19739) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 300.493 CPU sec, 19.102 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3817, 388111, 3704, 3935) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1391, 3382, 5857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14789) [M::mem_pestat] mean and std.dev: (3859.38, 2804.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19255) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 197, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (210.54, 86.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1671, 3638, 6323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15627) [M::mem_pestat] mean and std.dev: (4103.54, 2794.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20279) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1559, 3505, 6139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15299) [M::mem_pestat] mean and std.dev: (3990.57, 2759.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19879) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 304.218 CPU sec, 19.567 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4057, 387081, 3600, 3924) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1433, 3413, 6135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15539) [M::mem_pestat] mean and std.dev: (3937.68, 2834.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 197, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (210.43, 86.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1604, 3503, 6260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15572) [M::mem_pestat] mean and std.dev: (4053.93, 2800.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20228) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1588, 3597, 6279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15661) [M::mem_pestat] mean and std.dev: (4065.56, 2801.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20352) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 304.242 CPU sec, 19.343 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4094, 383813, 3806, 3851) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1315, 3255, 5915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15115) [M::mem_pestat] mean and std.dev: (3780.24, 2807.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (209.04, 85.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1604, 3653, 6238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15506) [M::mem_pestat] mean and std.dev: (4054.08, 2793.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20140) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1603, 3601, 6237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15505) [M::mem_pestat] mean and std.dev: (4062.06, 2786.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20139) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 322.050 CPU sec, 20.562 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4067, 388122, 3844, 3890) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1319, 3269, 5982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15308) [M::mem_pestat] mean and std.dev: (3821.71, 2828.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19971) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (209.99, 86.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1608, 3487, 6002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14790) [M::mem_pestat] mean and std.dev: (3963.12, 2750.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19184) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1571, 3677, 6207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15479) [M::mem_pestat] mean and std.dev: (4029.16, 2755.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20115) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 298.331 CPU sec, 19.018 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4090, 388943, 3607, 3876) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1340, 3369, 5962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15206) [M::mem_pestat] mean and std.dev: (3844.77, 2809.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19828) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (208.74, 85.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1665, 3620, 6291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15543) [M::mem_pestat] mean and std.dev: (4115.96, 2793.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20169) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1604, 3636, 6248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15536) [M::mem_pestat] mean and std.dev: (4092.27, 2801.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20180) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 304.640 CPU sec, 19.415 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4111, 389392, 3672, 3771) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1399, 3451, 6157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15673) [M::mem_pestat] mean and std.dev: (3924.99, 2854.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 194, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (208.24, 85.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1515, 3418, 6008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14994) [M::mem_pestat] mean and std.dev: (3966.55, 2806.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19487) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1641, 3591, 6139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15135) [M::mem_pestat] mean and std.dev: (4030.28, 2738.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19633) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 304.393 CPU sec, 19.410 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4019, 390484, 3649, 3820) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1350, 3348, 6120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15660) [M::mem_pestat] mean and std.dev: (3890.80, 2849.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 194, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (207.55, 85.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1503, 3519, 6295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15879) [M::mem_pestat] mean and std.dev: (4018.63, 2824.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20671) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1597, 3505, 6110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15136) [M::mem_pestat] mean and std.dev: (4004.41, 2748.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19649) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 307.716 CPU sec, 19.642 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3945, 391835, 3840, 3979) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1293, 3285, 5994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15396) [M::mem_pestat] mean and std.dev: (3789.03, 2840.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20097) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 192, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (206.51, 84.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1629, 3649, 6366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15840) [M::mem_pestat] mean and std.dev: (4101.97, 2806.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20577) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1667, 3656, 6284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15518) [M::mem_pestat] mean and std.dev: (4067.90, 2758.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20135) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 299.296 CPU sec, 19.106 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4190, 383480, 3723, 3964) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1325, 3371, 5980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15290) [M::mem_pestat] mean and std.dev: (3829.35, 2827.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 197, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (210.81, 86.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1631, 3618, 6299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15635) [M::mem_pestat] mean and std.dev: (4076.13, 2777.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20303) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1521, 3414, 6005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14973) [M::mem_pestat] mean and std.dev: (3919.24, 2736.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19457) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 325.954 CPU sec, 20.803 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4037, 384732, 3717, 3855) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1407, 3429, 6081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15429) [M::mem_pestat] mean and std.dev: (3882.42, 2834.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20103) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 197, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (211.16, 86.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1600, 3541, 6209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15427) [M::mem_pestat] mean and std.dev: (4013.91, 2767.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20036) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1683, 3673, 6295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15519) [M::mem_pestat] mean and std.dev: (4111.32, 2787.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20131) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 309.414 CPU sec, 19.701 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4056, 385482, 3677, 3881) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1338, 3376, 6031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15417) [M::mem_pestat] mean and std.dev: (3859.43, 2846.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20110) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 198, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (211.53, 87.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1665, 3594, 6331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15663) [M::mem_pestat] mean and std.dev: (4108.94, 2797.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20329) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1578, 3601, 6188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15408) [M::mem_pestat] mean and std.dev: (4043.66, 2786.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20018) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 311.026 CPU sec, 19.885 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3954, 384922, 3760, 4006) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1421, 3568, 6044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15290) [M::mem_pestat] mean and std.dev: (3934.98, 2825.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19913) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 197, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (211.41, 87.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1571, 3571, 6286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15716) [M::mem_pestat] mean and std.dev: (4068.19, 2817.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20431) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1591, 3631, 6195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15403) [M::mem_pestat] mean and std.dev: (4056.12, 2788.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20007) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 311.658 CPU sec, 19.797 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4064, 384841, 3827, 4018) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1442, 3354, 5964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15008) [M::mem_pestat] mean and std.dev: (3863.48, 2790.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 197, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (211.18, 87.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1636, 3596, 6290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15598) [M::mem_pestat] mean and std.dev: (4094.32, 2822.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20252) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1587, 3573, 6160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15306) [M::mem_pestat] mean and std.dev: (4046.61, 2804.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19879) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 313.169 CPU sec, 20.085 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3946, 365467, 3708, 3768) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1341, 3316, 6179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15855) [M::mem_pestat] mean and std.dev: (3875.46, 2871.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (209.02, 85.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1603, 3555, 6089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15061) [M::mem_pestat] mean and std.dev: (4017.15, 2756.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19547) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1580, 3556, 6223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15509) [M::mem_pestat] mean and std.dev: (4024.53, 2770.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20152) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 335.262 CPU sec, 21.383 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3987, 387745, 3622, 3944) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1357, 3347, 5903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14995) [M::mem_pestat] mean and std.dev: (3815.57, 2789.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 196, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (210.21, 86.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1657, 3618, 6340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15706) [M::mem_pestat] mean and std.dev: (4070.71, 2772.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20389) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1580, 3566, 6188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15404) [M::mem_pestat] mean and std.dev: (4043.85, 2784.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20012) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 308.594 CPU sec, 19.581 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4019, 389519, 3655, 3968) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1403, 3338, 6083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15443) [M::mem_pestat] mean and std.dev: (3888.85, 2835.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20123) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (209.91, 86.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1615, 3547, 6288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15634) [M::mem_pestat] mean and std.dev: (4066.95, 2788.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1575, 3530, 6130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15240) [M::mem_pestat] mean and std.dev: (3999.57, 2787.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19795) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 302.353 CPU sec, 19.442 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3926, 387220, 3722, 3911) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1372, 3393, 5994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15238) [M::mem_pestat] mean and std.dev: (3883.94, 2822.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (209.48, 86.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1595, 3488, 6075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15035) [M::mem_pestat] mean and std.dev: (3980.06, 2756.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19515) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1636, 3633, 6177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15259) [M::mem_pestat] mean and std.dev: (4058.54, 2745.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19800) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 298.333 CPU sec, 18.984 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4033, 389531, 3654, 4017) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1292, 3203, 5898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15110) [M::mem_pestat] mean and std.dev: (3775.94, 2810.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (208.83, 86.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1691, 3708, 6345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15653) [M::mem_pestat] mean and std.dev: (4150.24, 2827.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1619, 3651, 6318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15716) [M::mem_pestat] mean and std.dev: (4091.72, 2775.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20415) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 297.502 CPU sec, 19.027 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4015, 389632, 3786, 3935) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1438, 3411, 6197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15715) [M::mem_pestat] mean and std.dev: (3925.96, 2841.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (208.76, 85.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1663, 3681, 6316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15622) [M::mem_pestat] mean and std.dev: (4111.29, 2813.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20275) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1636, 3605, 6220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15388) [M::mem_pestat] mean and std.dev: (4065.15, 2782.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19972) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 322.784 CPU sec, 20.640 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4031, 382832, 3778, 3948) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1332, 3312, 6160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15816) [M::mem_pestat] mean and std.dev: (3862.18, 2885.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (209.57, 86.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1645, 3845, 6373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15829) [M::mem_pestat] mean and std.dev: (4168.61, 2819.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20557) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1554, 3450, 5985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14847) [M::mem_pestat] mean and std.dev: (3928.88, 2732.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19278) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 320.509 CPU sec, 20.370 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4061, 383475, 3637, 3821) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1337, 3368, 6079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15563) [M::mem_pestat] mean and std.dev: (3865.74, 2850.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 198, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (211.61, 87.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1584, 3576, 6278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15666) [M::mem_pestat] mean and std.dev: (4065.80, 2826.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20360) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1594, 3488, 6022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14878) [M::mem_pestat] mean and std.dev: (3954.90, 2733.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19306) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 308.905 CPU sec, 19.677 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4025, 384708, 3678, 4043) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1375, 3299, 6018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15304) [M::mem_pestat] mean and std.dev: (3860.71, 2842.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 198, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (211.35, 86.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1623, 3533, 6350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15804) [M::mem_pestat] mean and std.dev: (4096.71, 2808.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20531) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1537, 3470, 6230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15616) [M::mem_pestat] mean and std.dev: (3993.70, 2759.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20309) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 306.978 CPU sec, 19.516 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3914, 386204, 3758, 3926) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1393, 3335, 6086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15472) [M::mem_pestat] mean and std.dev: (3865.87, 2817.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20165) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 198, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (211.57, 87.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1562, 3658, 6369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15983) [M::mem_pestat] mean and std.dev: (4102.96, 2842.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20790) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1606, 3608, 6190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15358) [M::mem_pestat] mean and std.dev: (4070.38, 2799.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19942) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 299.763 CPU sec, 19.085 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4049, 386322, 3722, 3935) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1384, 3373, 5932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15028) [M::mem_pestat] mean and std.dev: (3827.03, 2760.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 197, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (211.13, 87.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1612, 3627, 6336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15784) [M::mem_pestat] mean and std.dev: (4108.60, 2819.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20508) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1580, 3486, 6137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15251) [M::mem_pestat] mean and std.dev: (3995.94, 2760.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19808) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 305.307 CPU sec, 19.485 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4064, 387215, 3838, 3897) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1393, 3382, 6035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15319) [M::mem_pestat] mean and std.dev: (3879.01, 2831.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 197, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523) [M::mem_pestat] mean and std.dev: (210.89, 86.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1609, 3637, 6362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15868) [M::mem_pestat] mean and std.dev: (4085.27, 2812.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20621) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1540, 3406, 6069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15127) [M::mem_pestat] mean and std.dev: (3953.16, 2759.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19656) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 299.632 CPU sec, 19.111 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3962, 385388, 3654, 3977) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1459, 3414, 5965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14977) [M::mem_pestat] mean and std.dev: (3917.97, 2814.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (209.68, 86.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1648, 3720, 6371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15817) [M::mem_pestat] mean and std.dev: (4146.86, 2822.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20540) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1547, 3494, 6209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15533) [M::mem_pestat] mean and std.dev: (4009.76, 2776.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20195) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 307.078 CPU sec, 19.546 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4087, 388007, 3793, 3811) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1370, 3396, 6025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15335) [M::mem_pestat] mean and std.dev: (3864.75, 2805.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19990) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (209.87, 86.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1653, 3568, 6146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15132) [M::mem_pestat] mean and std.dev: (4040.36, 2775.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19625) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1602, 3511, 6137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15207) [M::mem_pestat] mean and std.dev: (4013.12, 2752.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19742) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 294.828 CPU sec, 18.807 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3976, 387964, 3729, 3978) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1411, 3360, 6022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15244) [M::mem_pestat] mean and std.dev: (3883.36, 2819.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (209.90, 86.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1581, 3473, 6188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15402) [M::mem_pestat] mean and std.dev: (3988.14, 2778.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20009) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1638, 3734, 6175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15249) [M::mem_pestat] mean and std.dev: (4090.78, 2756.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19786) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 305.597 CPU sec, 19.441 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4089, 386253, 3738, 3988) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1360, 3355, 6064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15472) [M::mem_pestat] mean and std.dev: (3859.56, 2824.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20176) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (209.61, 86.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1617, 3536, 6320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15726) [M::mem_pestat] mean and std.dev: (4061.58, 2809.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20429) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1556, 3472, 6174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15410) [M::mem_pestat] mean and std.dev: (4001.93, 2789.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20028) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 324.204 CPU sec, 20.752 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3980, 389201, 3682, 3840) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1323, 3245, 6005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15369) [M::mem_pestat] mean and std.dev: (3820.33, 2836.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20051) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (208.65, 85.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1646, 3609, 6381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15851) [M::mem_pestat] mean and std.dev: (4089.04, 2804.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20586) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1593, 3574, 6108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15138) [M::mem_pestat] mean and std.dev: (4010.79, 2752.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19653) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 315.944 CPU sec, 20.057 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3881, 389176, 3688, 3892) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1411, 3421, 6108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15502) [M::mem_pestat] mean and std.dev: (3936.14, 2849.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20199) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 192, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (205.77, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1580, 3498, 6307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15761) [M::mem_pestat] mean and std.dev: (4010.74, 2811.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20488) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1545, 3613, 6119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15267) [M::mem_pestat] mean and std.dev: (4041.02, 2788.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19841) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 309.132 CPU sec, 19.784 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4080, 381267, 4009, 4242) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1492, 3372, 6029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15103) [M::mem_pestat] mean and std.dev: (3880.50, 2773.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (196.40, 76.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1516, 3427, 6143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15397) [M::mem_pestat] mean and std.dev: (3978.80, 2803.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20024) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1509, 3358, 6001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14985) [M::mem_pestat] mean and std.dev: (3895.11, 2768.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19477) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 317.422 CPU sec, 20.165 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4267, 380318, 4095, 4128) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1496, 3345, 5914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14750) [M::mem_pestat] mean and std.dev: (3841.60, 2736.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19168) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 184, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (195.92, 76.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1577, 3523, 6163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15335) [M::mem_pestat] mean and std.dev: (3992.35, 2797.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19921) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1532, 3409, 6170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15446) [M::mem_pestat] mean and std.dev: (3969.74, 2781.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20084) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 323.295 CPU sec, 20.633 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4087, 374352, 4171, 4141) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1322, 3189, 5974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15278) [M::mem_pestat] mean and std.dev: (3793.53, 2815.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 184, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (195.59, 75.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1506, 3475, 6156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15456) [M::mem_pestat] mean and std.dev: (3966.29, 2805.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20106) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1594, 3517, 6109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15139) [M::mem_pestat] mean and std.dev: (4006.16, 2777.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19654) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 323.549 CPU sec, 20.701 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4142, 376359, 4051, 4151) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1405, 3486, 6193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15769) [M::mem_pestat] mean and std.dev: (3974.73, 2862.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 184, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (195.13, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1516, 3411, 6073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15187) [M::mem_pestat] mean and std.dev: (3931.52, 2785.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19744) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1570, 3465, 6111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15193) [M::mem_pestat] mean and std.dev: (3997.15, 2771.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19734) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 323.749 CPU sec, 20.761 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4164, 377948, 4083, 4290) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1425, 3363, 5898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14844) [M::mem_pestat] mean and std.dev: (3853.70, 2760.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19317) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (196.25, 76.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1551, 3529, 6297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15789) [M::mem_pestat] mean and std.dev: (4025.27, 2828.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20535) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1534, 3436, 6041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15055) [M::mem_pestat] mean and std.dev: (3933.87, 2753.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19562) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 316.235 CPU sec, 20.065 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4146, 376566, 3990, 4066) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1385, 3243, 5932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15026) [M::mem_pestat] mean and std.dev: (3802.49, 2771.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 184, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (195.68, 76.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1569, 3583, 6288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15726) [M::mem_pestat] mean and std.dev: (4037.48, 2793.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20445) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1513, 3512, 6202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15580) [M::mem_pestat] mean and std.dev: (3993.52, 2776.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20269) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 319.397 CPU sec, 20.437 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4155, 378653, 4175, 4075) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1399, 3356, 5959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15079) [M::mem_pestat] mean and std.dev: (3865.44, 2797.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (196.98, 76.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1479, 3379, 6028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15126) [M::mem_pestat] mean and std.dev: (3893.89, 2779.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19675) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1513, 3450, 5954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14836) [M::mem_pestat] mean and std.dev: (3939.70, 2773.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 314.435 CPU sec, 19.961 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4277, 380105, 3902, 4333) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1466, 3381, 5963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14957) [M::mem_pestat] mean and std.dev: (3889.24, 2784.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (196.91, 77.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1485, 3326, 6030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15120) [M::mem_pestat] mean and std.dev: (3888.05, 2769.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19665) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1597, 3630, 6164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15298) [M::mem_pestat] mean and std.dev: (4036.15, 2771.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19865) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 309.794 CPU sec, 19.758 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4289, 375719, 4015, 4213) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1330, 3237, 5861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14923) [M::mem_pestat] mean and std.dev: (3783.15, 2776.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 186, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (198.05, 77.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1549, 3502, 6261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15685) [M::mem_pestat] mean and std.dev: (4019.62, 2822.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20397) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1507, 3592, 6217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15637) [M::mem_pestat] mean and std.dev: (4010.89, 2800.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20347) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 315.614 CPU sec, 20.165 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4041, 373268, 4001, 4092) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1457, 3382, 6003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15095) [M::mem_pestat] mean and std.dev: (3901.77, 2815.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (197.30, 77.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1562, 3487, 6168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15380) [M::mem_pestat] mean and std.dev: (3995.25, 2817.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19986) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1508, 3477, 6226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15662) [M::mem_pestat] mean and std.dev: (4005.11, 2821.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20380) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 320.469 CPU sec, 20.462 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4255, 374905, 4094, 4120) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1464, 3425, 6121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15435) [M::mem_pestat] mean and std.dev: (3952.53, 2811.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20092) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 187, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (198.74, 78.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1519, 3429, 6057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15133) [M::mem_pestat] mean and std.dev: (3946.59, 2783.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19671) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1534, 3449, 6027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15013) [M::mem_pestat] mean and std.dev: (3941.59, 2777.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19506) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 310.729 CPU sec, 19.760 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4132, 374155, 3915, 4009) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1421, 3283, 5969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15065) [M::mem_pestat] mean and std.dev: (3843.01, 2796.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (195.06, 76.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1510, 3520, 6247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15721) [M::mem_pestat] mean and std.dev: (3991.39, 2792.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20458) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1535, 3460, 6086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15188) [M::mem_pestat] mean and std.dev: (3981.02, 2768.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19739) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 334.615 CPU sec, 21.282 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4208, 376070, 4046, 4079) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1428, 3425, 6044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15276) [M::mem_pestat] mean and std.dev: (3888.80, 2789.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (195.08, 75.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1590, 3501, 6213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15459) [M::mem_pestat] mean and std.dev: (4037.71, 2800.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20082) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1555, 3512, 6185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15445) [M::mem_pestat] mean and std.dev: (4014.24, 2768.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20075) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 327.543 CPU sec, 21.004 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4070, 366589, 3862, 4065) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1375, 3458, 6167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15751) [M::mem_pestat] mean and std.dev: (3927.75, 2853.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (194.28, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1486, 3472, 5955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14893) [M::mem_pestat] mean and std.dev: (3921.27, 2771.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19362) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1515, 3438, 5865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14565) [M::mem_pestat] mean and std.dev: (3920.28, 2731.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18915) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 332.701 CPU sec, 21.179 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4005, 363932, 3896, 4038) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1407, 3368, 6019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15243) [M::mem_pestat] mean and std.dev: (3883.02, 2836.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 182, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (193.98, 75.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1517, 3544, 6110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15296) [M::mem_pestat] mean and std.dev: (3979.24, 2789.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19889) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1497, 3460, 6126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15384) [M::mem_pestat] mean and std.dev: (3968.50, 2795.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20013) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 334.668 CPU sec, 21.201 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4119, 371022, 3964, 4059) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1403, 3411, 6039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15311) [M::mem_pestat] mean and std.dev: (3896.06, 2805.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 184, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (195.30, 75.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1493, 3494, 6210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15644) [M::mem_pestat] mean and std.dev: (4004.69, 2845.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20361) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1495, 3381, 6106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15328) [M::mem_pestat] mean and std.dev: (3952.90, 2796.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 329.303 CPU sec, 21.115 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4120, 364931, 3942, 4043) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1397, 3381, 6150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15656) [M::mem_pestat] mean and std.dev: (3932.68, 2849.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (138, 183, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (194.64, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1505, 3477, 6199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15587) [M::mem_pestat] mean and std.dev: (3986.13, 2812.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20281) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1606, 3675, 6238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15502) [M::mem_pestat] mean and std.dev: (4079.52, 2778.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20134) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 334.532 CPU sec, 21.252 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3993, 370674, 3833, 4227) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1351, 3323, 6002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15304) [M::mem_pestat] mean and std.dev: (3815.64, 2784.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 184, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (196.45, 76.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1562, 3476, 6170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15386) [M::mem_pestat] mean and std.dev: (3982.16, 2777.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19994) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1564, 3486, 6007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14893) [M::mem_pestat] mean and std.dev: (3955.11, 2747.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19336) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 322.032 CPU sec, 20.615 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4137, 370622, 4012, 4120) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1498, 3381, 6078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15238) [M::mem_pestat] mean and std.dev: (3927.28, 2805.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (197.29, 77.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1535, 3499, 6065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15125) [M::mem_pestat] mean and std.dev: (3971.61, 2780.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19655) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1540, 3501, 6003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14929) [M::mem_pestat] mean and std.dev: (3965.13, 2756.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19392) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 320.335 CPU sec, 20.481 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4203, 369170, 4048, 4116) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1452, 3354, 6135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15501) [M::mem_pestat] mean and std.dev: (3931.21, 2861.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20184) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 186, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (197.96, 78.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1546, 3472, 6133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15307) [M::mem_pestat] mean and std.dev: (3961.27, 2802.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19894) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1447, 3313, 6084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15358) [M::mem_pestat] mean and std.dev: (3927.48, 2817.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19995) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 317.715 CPU sec, 20.196 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4082, 364274, 3926, 4001) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1446, 3345, 5918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14862) [M::mem_pestat] mean and std.dev: (3852.10, 2773.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 186, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (197.76, 78.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1469, 3426, 6145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15497) [M::mem_pestat] mean and std.dev: (3927.72, 2815.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20173) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1626, 3533, 6173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15267) [M::mem_pestat] mean and std.dev: (4042.00, 2762.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19814) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 321.609 CPU sec, 20.534 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4115, 362327, 3874, 4073) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1411, 3329, 6037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15289) [M::mem_pestat] mean and std.dev: (3864.84, 2817.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19915) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 186, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (197.60, 78.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1573, 3498, 6143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15283) [M::mem_pestat] mean and std.dev: (3995.72, 2778.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19853) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1543, 3613, 6240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15634) [M::mem_pestat] mean and std.dev: (4056.46, 2824.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20331) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 321.376 CPU sec, 20.663 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4440, 373572, 4131, 4325) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1403, 3423, 6037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15305) [M::mem_pestat] mean and std.dev: (3870.29, 2808.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19939) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (196.83, 76.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1517, 3376, 5935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14771) [M::mem_pestat] mean and std.dev: (3907.47, 2744.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19189) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1477, 3408, 6186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15604) [M::mem_pestat] mean and std.dev: (3954.31, 2784.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20313) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 332.721 CPU sec, 21.165 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4466, 371784, 4028, 4281) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1444, 3430, 6065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15307) [M::mem_pestat] mean and std.dev: (3901.91, 2807.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19928) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (196.63, 76.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1421, 3401, 6190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15728) [M::mem_pestat] mean and std.dev: (3923.43, 2842.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20497) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1528, 3433, 6182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15490) [M::mem_pestat] mean and std.dev: (3985.59, 2820.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20144) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 346.673 CPU sec, 22.117 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4406, 368584, 4205, 4173) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1416, 3406, 5988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15132) [M::mem_pestat] mean and std.dev: (3864.13, 2781.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (196.26, 76.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1545, 3496, 6238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15624) [M::mem_pestat] mean and std.dev: (4012.42, 2797.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20317) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1464, 3438, 6066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15270) [M::mem_pestat] mean and std.dev: (3958.66, 2813.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 334.221 CPU sec, 21.462 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4249, 371379, 4150, 4090) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1430, 3272, 5929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14927) [M::mem_pestat] mean and std.dev: (3851.24, 2790.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (196.65, 76.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1505, 3501, 6221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15653) [M::mem_pestat] mean and std.dev: (3993.95, 2819.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20369) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1550, 3557, 6080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15140) [M::mem_pestat] mean and std.dev: (3972.37, 2753.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19670) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 349.306 CPU sec, 22.287 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4384, 373525, 4255, 4193) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1382, 3336, 5899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14933) [M::mem_pestat] mean and std.dev: (3817.60, 2789.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 186, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (197.45, 76.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1472, 3396, 5931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14849) [M::mem_pestat] mean and std.dev: (3878.44, 2758.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19308) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1554, 3612, 6294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15774) [M::mem_pestat] mean and std.dev: (4057.95, 2808.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20514) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 323.546 CPU sec, 20.603 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4322, 368454, 4144, 4218) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1393, 3331, 6040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15334) [M::mem_pestat] mean and std.dev: (3845.79, 2798.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 186, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (197.47, 77.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1519, 3576, 6275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15787) [M::mem_pestat] mean and std.dev: (4020.27, 2834.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20543) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1537, 3420, 6019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14983) [M::mem_pestat] mean and std.dev: (3944.95, 2780.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19465) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 329.648 CPU sec, 21.014 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4382, 373219, 4114, 4332) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1414, 3373, 6032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15268) [M::mem_pestat] mean and std.dev: (3844.62, 2800.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 186, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (198.11, 77.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1530, 3560, 6110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15270) [M::mem_pestat] mean and std.dev: (3994.18, 2788.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19850) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1620, 3513, 6203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15369) [M::mem_pestat] mean and std.dev: (4042.01, 2798.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19952) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 321.304 CPU sec, 20.464 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4497, 371585, 4229, 4283) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1361, 3308, 5864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14870) [M::mem_pestat] mean and std.dev: (3826.55, 2787.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 187, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (198.41, 77.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1469, 3481, 6041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15185) [M::mem_pestat] mean and std.dev: (3940.77, 2795.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19757) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1552, 3456, 6075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15121) [M::mem_pestat] mean and std.dev: (3973.37, 2773.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19644) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 321.348 CPU sec, 20.933 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4350, 371321, 4202, 4226) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1368, 3388, 5983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15213) [M::mem_pestat] mean and std.dev: (3884.36, 2827.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19828) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 187, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (199.25, 78.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1558, 3571, 6158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15358) [M::mem_pestat] mean and std.dev: (3985.65, 2791.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19958) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1497, 3367, 6083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15255) [M::mem_pestat] mean and std.dev: (3926.31, 2771.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19841) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 318.540 CPU sec, 20.278 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4507, 369419, 4149, 4203) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1378, 3324, 5921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15007) [M::mem_pestat] mean and std.dev: (3836.57, 2796.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 188, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (199.38, 78.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1521, 3520, 6220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15618) [M::mem_pestat] mean and std.dev: (4011.13, 2808.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20317) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1561, 3423, 6147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15319) [M::mem_pestat] mean and std.dev: (3992.27, 2793.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19905) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 325.922 CPU sec, 20.858 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4243, 372945, 4262, 4321) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1469, 3381, 6071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15275) [M::mem_pestat] mean and std.dev: (3923.07, 2823.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 187, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (199.22, 78.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1557, 3507, 6161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15369) [M::mem_pestat] mean and std.dev: (4027.04, 2815.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19973) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1556, 3580, 6331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15881) [M::mem_pestat] mean and std.dev: (4072.59, 2837.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20656) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 318.388 CPU sec, 20.166 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4470, 376775, 4199, 4200) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1316, 3365, 5972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15284) [M::mem_pestat] mean and std.dev: (3844.52, 2820.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (196.62, 76.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1479, 3468, 6116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15390) [M::mem_pestat] mean and std.dev: (3947.79, 2780.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20027) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1585, 3541, 6032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14926) [M::mem_pestat] mean and std.dev: (3973.08, 2742.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19373) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 331.933 CPU sec, 21.147 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4300, 371663, 4158, 4253) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1364, 3396, 5997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15263) [M::mem_pestat] mean and std.dev: (3876.85, 2832.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (196.13, 76.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1526, 3457, 6116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15296) [M::mem_pestat] mean and std.dev: (3949.54, 2780.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19886) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1522, 3482, 6071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15169) [M::mem_pestat] mean and std.dev: (3972.56, 2771.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19718) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 343.406 CPU sec, 22.045 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4403, 368181, 4204, 4170) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1441, 3336, 6078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15352) [M::mem_pestat] mean and std.dev: (3898.09, 2820.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (196.82, 76.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1494, 3446, 6211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15645) [M::mem_pestat] mean and std.dev: (3973.92, 2836.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20362) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1586, 3520, 6192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15404) [M::mem_pestat] mean and std.dev: (4049.59, 2809.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20010) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 333.895 CPU sec, 21.185 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4429, 368450, 4100, 4139) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1415, 3353, 6072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15386) [M::mem_pestat] mean and std.dev: (3903.91, 2832.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20043) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 185, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (196.92, 76.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1573, 3462, 6132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15250) [M::mem_pestat] mean and std.dev: (3980.96, 2803.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19809) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1542, 3553, 6211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15549) [M::mem_pestat] mean and std.dev: (4016.97, 2771.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20218) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 334.115 CPU sec, 21.247 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4282, 375607, 4101, 4213) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1361, 3321, 6041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15401) [M::mem_pestat] mean and std.dev: (3842.84, 2812.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 186, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (197.57, 76.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1524, 3454, 6199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15549) [M::mem_pestat] mean and std.dev: (3979.64, 2812.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20224) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1574, 3613, 6154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15314) [M::mem_pestat] mean and std.dev: (4031.27, 2776.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19894) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 322.397 CPU sec, 20.669 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4283, 369666, 4171, 4079) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1470, 3492, 5990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15030) [M::mem_pestat] mean and std.dev: (3929.72, 2803.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (139, 186, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (197.46, 77.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1571, 3537, 6127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15239) [M::mem_pestat] mean and std.dev: (4001.52, 2775.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19795) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1577, 3625, 6136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15254) [M::mem_pestat] mean and std.dev: (4038.67, 2775.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19813) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 332.986 CPU sec, 21.357 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4219, 374282, 4095, 4112) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1397, 3383, 6029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15293) [M::mem_pestat] mean and std.dev: (3898.09, 2821.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19925) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 186, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (198.20, 77.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1451, 3465, 6041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15221) [M::mem_pestat] mean and std.dev: (3936.00, 2796.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19811) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1571, 3476, 5961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14741) [M::mem_pestat] mean and std.dev: (3958.81, 2739.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19131) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 319.339 CPU sec, 20.314 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4382, 369345, 4195, 4297) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1388, 3399, 6151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15677) [M::mem_pestat] mean and std.dev: (3923.54, 2854.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 187, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (198.93, 78.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1534, 3414, 6100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15232) [M::mem_pestat] mean and std.dev: (3958.31, 2804.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19798) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1539, 3392, 6069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15129) [M::mem_pestat] mean and std.dev: (3965.77, 2780.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19659) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 323.479 CPU sec, 20.759 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4303, 370696, 4195, 4358) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] (25, 50, 75) percentile: (1443, 3448, 6131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15507) [M::mem_pestat] mean and std.dev: (3930.82, 2810.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20195) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 187, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (199.21, 78.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1539, 3434, 6195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15507) [M::mem_pestat] mean and std.dev: (3986.80, 2830.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20163) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1502, 3439, 6105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15311) [M::mem_pestat] mean and std.dev: (3972.37, 2820.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19914) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 324.187 CPU sec, 20.583 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4288, 372613, 4192, 4482) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1389, 3262, 5857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14793) [M::mem_pestat] mean and std.dev: (3798.77, 2795.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (141, 189, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (201.11, 79.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1570, 3608, 6122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15226) [M::mem_pestat] mean and std.dev: (3999.97, 2775.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19778) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1528, 3603, 6192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15520) [M::mem_pestat] mean and std.dev: (4044.57, 2796.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20184) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 316.727 CPU sec, 20.250 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4530, 372275, 4304, 4439) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1510, 3497, 6161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15463) [M::mem_pestat] mean and std.dev: (3963.63, 2815.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20114) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 193, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (205.11, 82.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1544, 3395, 5985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14867) [M::mem_pestat] mean and std.dev: (3919.82, 2755.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19308) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1417, 3364, 6111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15499) [M::mem_pestat] mean and std.dev: (3918.07, 2806.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20193) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 309.329 CPU sec, 19.707 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4713, 370651, 4281, 4774) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1424, 3326, 5944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14984) [M::mem_pestat] mean and std.dev: (3835.08, 2756.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (208.91, 85.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1468, 3460, 6130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15454) [M::mem_pestat] mean and std.dev: (3970.88, 2811.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20116) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1548, 3482, 6321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15867) [M::mem_pestat] mean and std.dev: (4031.30, 2818.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20640) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 303.641 CPU sec, 19.330 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4542, 368796, 4322, 4563) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1557, 3484, 6032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14982) [M::mem_pestat] mean and std.dev: (3967.45, 2776.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (208.97, 85.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1520, 3576, 6106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15278) [M::mem_pestat] mean and std.dev: (4001.68, 2800.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19864) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1656, 3592, 6131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15081) [M::mem_pestat] mean and std.dev: (4026.87, 2737.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19556) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 308.725 CPU sec, 19.754 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4583, 369901, 4461, 4493) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1568, 3427, 5918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14618) [M::mem_pestat] mean and std.dev: (3890.63, 2735.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (208.27, 85.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1506, 3497, 6153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15447) [M::mem_pestat] mean and std.dev: (3982.03, 2802.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20094) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1515, 3384, 6020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15030) [M::mem_pestat] mean and std.dev: (3926.95, 2776.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19535) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 304.933 CPU sec, 19.389 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4528, 369956, 4392, 4651) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1476, 3430, 6037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15159) [M::mem_pestat] mean and std.dev: (3930.20, 2797.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (208.31, 85.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1517, 3432, 6137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15377) [M::mem_pestat] mean and std.dev: (3959.44, 2793.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19997) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1484, 3385, 6104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15344) [M::mem_pestat] mean and std.dev: (3963.87, 2798.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19964) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 303.057 CPU sec, 19.331 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4725, 370447, 4566, 4474) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1422, 3235, 5786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14514) [M::mem_pestat] mean and std.dev: (3776.20, 2733.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (208.11, 85.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1572, 3582, 6222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15522) [M::mem_pestat] mean and std.dev: (4040.66, 2782.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20172) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1576, 3449, 6077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15079) [M::mem_pestat] mean and std.dev: (3970.68, 2772.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19580) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 306.002 CPU sec, 19.562 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4536, 370040, 4395, 4557) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1497, 3519, 6163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15495) [M::mem_pestat] mean and std.dev: (3988.94, 2811.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (208.29, 85.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1504, 3350, 5976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14920) [M::mem_pestat] mean and std.dev: (3916.73, 2778.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19392) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1605, 3577, 6221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15453) [M::mem_pestat] mean and std.dev: (4035.68, 2775.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20069) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 304.705 CPU sec, 19.507 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4672, 369105, 4425, 4473) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1435, 3384, 5977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15061) [M::mem_pestat] mean and std.dev: (3878.44, 2776.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 194, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (206.95, 84.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1450, 3368, 6029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15187) [M::mem_pestat] mean and std.dev: (3876.65, 2784.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19766) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1593, 3492, 6175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15339) [M::mem_pestat] mean and std.dev: (4003.00, 2768.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19921) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 316.357 CPU sec, 20.137 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4517, 372010, 4470, 4657) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1449, 3417, 6000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15102) [M::mem_pestat] mean and std.dev: (3891.73, 2766.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (207.57, 85.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1447, 3423, 6032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15202) [M::mem_pestat] mean and std.dev: (3884.86, 2751.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19787) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1520, 3483, 6126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15338) [M::mem_pestat] mean and std.dev: (3986.48, 2794.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19944) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 302.148 CPU sec, 19.279 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4682, 370805, 4483, 4419) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1519, 3384, 5936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14770) [M::mem_pestat] mean and std.dev: (3899.78, 2775.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19187) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (207.33, 84.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1557, 3557, 6252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15642) [M::mem_pestat] mean and std.dev: (4016.51, 2806.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20337) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1531, 3465, 6084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15190) [M::mem_pestat] mean and std.dev: (3964.77, 2747.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19743) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 305.623 CPU sec, 19.492 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4505, 372524, 4270, 4472) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1521, 3308, 5986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14916) [M::mem_pestat] mean and std.dev: (3876.26, 2742.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 194, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (206.73, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1456, 3446, 6148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15532) [M::mem_pestat] mean and std.dev: (3934.81, 2799.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20224) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1601, 3476, 6069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15005) [M::mem_pestat] mean and std.dev: (3994.15, 2753.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19473) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 303.557 CPU sec, 19.409 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4567, 372371, 4446, 4476) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1478, 3383, 5993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15023) [M::mem_pestat] mean and std.dev: (3890.90, 2777.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 193, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (206.09, 84.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1519, 3482, 6010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14992) [M::mem_pestat] mean and std.dev: (3942.20, 2761.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19483) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1509, 3353, 6024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15054) [M::mem_pestat] mean and std.dev: (3912.73, 2764.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19569) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 303.460 CPU sec, 19.339 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4574, 373516, 4296, 4492) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1385, 3292, 5868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14834) [M::mem_pestat] mean and std.dev: (3805.15, 2789.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19317) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 192, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (205.25, 83.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1530, 3555, 6179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15477) [M::mem_pestat] mean and std.dev: (4021.38, 2810.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20126) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1611, 3589, 6060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14958) [M::mem_pestat] mean and std.dev: (4002.41, 2734.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19407) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 306.823 CPU sec, 19.622 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4592, 375687, 4292, 4439) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1456, 3397, 5966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14986) [M::mem_pestat] mean and std.dev: (3897.13, 2785.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 193, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (205.51, 84.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1581, 3487, 6030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14928) [M::mem_pestat] mean and std.dev: (3971.85, 2755.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19377) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1519, 3389, 6006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14980) [M::mem_pestat] mean and std.dev: (3938.36, 2755.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19467) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 302.447 CPU sec, 19.394 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4663, 372702, 4632, 4613) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1453, 3406, 6031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15187) [M::mem_pestat] mean and std.dev: (3909.09, 2809.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 194, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (206.39, 84.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1499, 3470, 6117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15353) [M::mem_pestat] mean and std.dev: (3955.58, 2791.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19971) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1569, 3536, 6063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15051) [M::mem_pestat] mean and std.dev: (3961.81, 2741.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19545) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 305.757 CPU sec, 19.460 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4552, 370282, 4444, 4630) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1405, 3305, 5938) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15004) [M::mem_pestat] mean and std.dev: (3834.49, 2784.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 196, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (208.94, 85.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1507, 3501, 6152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15442) [M::mem_pestat] mean and std.dev: (3986.04, 2798.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20087) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1543, 3417, 5883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14563) [M::mem_pestat] mean and std.dev: (3913.04, 2715.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 302.122 CPU sec, 19.207 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4690, 369417, 4431, 4620) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1352, 3280, 5794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14678) [M::mem_pestat] mean and std.dev: (3781.46, 2747.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19120) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 197, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (209.24, 86.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1469, 3480, 6160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15542) [M::mem_pestat] mean and std.dev: (3967.96, 2808.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20233) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1536, 3519, 6227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15609) [M::mem_pestat] mean and std.dev: (4033.85, 2788.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20300) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 306.864 CPU sec, 19.760 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4566, 369885, 4447, 4674) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1452, 3393, 5917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14847) [M::mem_pestat] mean and std.dev: (3857.46, 2731.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19312) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (208.60, 85.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1546, 3374, 5931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14701) [M::mem_pestat] mean and std.dev: (3880.00, 2718.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19086) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1560, 3512, 6216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15528) [M::mem_pestat] mean and std.dev: (4015.18, 2797.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20184) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 303.799 CPU sec, 19.322 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4579, 370255, 4557, 4682) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1397, 3284, 5976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15134) [M::mem_pestat] mean and std.dev: (3862.79, 2806.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (208.74, 85.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1630, 3559, 6182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15286) [M::mem_pestat] mean and std.dev: (4045.79, 2786.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19838) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1536, 3414, 5984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14880) [M::mem_pestat] mean and std.dev: (3920.53, 2751.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19328) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 299.723 CPU sec, 19.265 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4678, 369228, 4499, 4591) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1458, 3277, 5998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15078) [M::mem_pestat] mean and std.dev: (3889.88, 2793.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (208.43, 85.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1556, 3635, 6275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15713) [M::mem_pestat] mean and std.dev: (4038.56, 2789.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20432) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1600, 3443, 6082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15046) [M::mem_pestat] mean and std.dev: (3983.66, 2764.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19528) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 306.711 CPU sec, 19.442 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4657, 370445, 4453, 4573) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1577, 3485, 6033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14945) [M::mem_pestat] mean and std.dev: (3969.12, 2761.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (208.09, 85.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1519, 3390, 6099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15259) [M::mem_pestat] mean and std.dev: (3927.14, 2789.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19839) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1518, 3603, 6382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16110) [M::mem_pestat] mean and std.dev: (4068.31, 2842.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20974) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 303.208 CPU sec, 19.300 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4459, 367061, 4402, 4743) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1505, 3365, 5993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14969) [M::mem_pestat] mean and std.dev: (3889.26, 2764.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (206.93, 84.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1527, 3486, 6142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15372) [M::mem_pestat] mean and std.dev: (3998.13, 2802.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19987) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1562, 3506, 6010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14906) [M::mem_pestat] mean and std.dev: (3981.88, 2759.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19354) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 324.984 CPU sec, 20.907 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4584, 371421, 4300, 4582) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1390, 3355, 6032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15316) [M::mem_pestat] mean and std.dev: (3886.04, 2832.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19958) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (207.51, 85.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1454, 3398, 6051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15245) [M::mem_pestat] mean and std.dev: (3942.99, 2826.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19842) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1562, 3575, 6240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15596) [M::mem_pestat] mean and std.dev: (4050.63, 2803.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20274) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 304.127 CPU sec, 19.379 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4526, 370947, 4420, 4500) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1396, 3294, 5935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15013) [M::mem_pestat] mean and std.dev: (3847.49, 2794.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (207.13, 84.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1565, 3491, 6140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15290) [M::mem_pestat] mean and std.dev: (3992.79, 2794.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19865) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1524, 3400, 6037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15063) [M::mem_pestat] mean and std.dev: (3940.84, 2765.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19576) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 304.456 CPU sec, 19.490 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4720, 371594, 4299, 4587) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1443, 3320, 5937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14925) [M::mem_pestat] mean and std.dev: (3871.25, 2810.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 194, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (206.47, 84.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1581, 3572, 6139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15255) [M::mem_pestat] mean and std.dev: (4021.69, 2759.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19813) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1525, 3517, 6077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15181) [M::mem_pestat] mean and std.dev: (3977.92, 2762.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19733) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 308.634 CPU sec, 19.591 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4638, 372227, 4389, 4638) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1478, 3418, 6117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15395) [M::mem_pestat] mean and std.dev: (3943.31, 2819.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20034) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 194, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (206.27, 84.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1450, 3409, 6101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15403) [M::mem_pestat] mean and std.dev: (3893.05, 2784.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20054) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1548, 3468, 6093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15183) [M::mem_pestat] mean and std.dev: (3966.52, 2758.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19728) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 302.204 CPU sec, 19.290 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4452, 373552, 4267, 4404) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1435, 3312, 5892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14806) [M::mem_pestat] mean and std.dev: (3819.96, 2762.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 193, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (205.16, 83.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1572, 3492, 6080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15096) [M::mem_pestat] mean and std.dev: (3957.86, 2761.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19604) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1501, 3496, 6173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15517) [M::mem_pestat] mean and std.dev: (3980.35, 2773.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20189) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 313.713 CPU sec, 19.971 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4390, 375400, 4307, 4481) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1447, 3399, 5935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14911) [M::mem_pestat] mean and std.dev: (3873.20, 2733.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 192, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (204.47, 83.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1484, 3357, 6136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15440) [M::mem_pestat] mean and std.dev: (3947.57, 2815.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20092) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1582, 3440, 5929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14623) [M::mem_pestat] mean and std.dev: (3920.94, 2706.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18970) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 302.912 CPU sec, 19.438 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4601, 367810, 4381, 4636) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1328, 3206, 6007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15365) [M::mem_pestat] mean and std.dev: (3796.82, 2817.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (207.08, 84.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1478, 3397, 5992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15020) [M::mem_pestat] mean and std.dev: (3904.35, 2774.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19534) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1599, 3519, 6090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15072) [M::mem_pestat] mean and std.dev: (3991.75, 2754.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19563) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 326.794 CPU sec, 20.758 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4683, 369693, 4366, 4591) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1467, 3404, 6067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15267) [M::mem_pestat] mean and std.dev: (3932.02, 2810.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19867) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 197, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (209.64, 86.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1540, 3512, 6121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15283) [M::mem_pestat] mean and std.dev: (3991.40, 2812.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19864) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1539, 3487, 6182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15468) [M::mem_pestat] mean and std.dev: (4012.84, 2796.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20111) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 308.742 CPU sec, 19.768 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4667, 366778, 4350, 4512) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1427, 3358, 5974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15068) [M::mem_pestat] mean and std.dev: (3850.27, 2781.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 196, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (208.89, 85.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1504, 3466, 6227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15673) [M::mem_pestat] mean and std.dev: (3977.20, 2820.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20396) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1584, 3508, 6176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15360) [M::mem_pestat] mean and std.dev: (4032.70, 2792.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19952) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 318.088 CPU sec, 20.189 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4605, 368210, 4546, 4674) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1441, 3402, 6050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15268) [M::mem_pestat] mean and std.dev: (3919.94, 2812.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 197, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (209.46, 85.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1509, 3394, 6016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15030) [M::mem_pestat] mean and std.dev: (3926.76, 2767.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19537) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1462, 3379, 5952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14932) [M::mem_pestat] mean and std.dev: (3906.33, 2773.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19422) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 305.468 CPU sec, 19.509 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4738, 367546, 4480, 4632) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1601, 3535, 6154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15260) [M::mem_pestat] mean and std.dev: (3978.62, 2759.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19813) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (209.19, 86.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1471, 3424, 6080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15298) [M::mem_pestat] mean and std.dev: (3925.90, 2797.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19907) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1470, 3458, 6146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15498) [M::mem_pestat] mean and std.dev: (3963.05, 2790.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20174) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 310.679 CPU sec, 19.758 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4572, 369075, 4441, 4438) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1525, 3383, 6086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15208) [M::mem_pestat] mean and std.dev: (3938.31, 2792.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (209.04, 85.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1506, 3448, 6183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15537) [M::mem_pestat] mean and std.dev: (3964.75, 2799.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1552, 3485, 6003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14905) [M::mem_pestat] mean and std.dev: (3955.37, 2753.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19356) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 316.610 CPU sec, 20.155 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4616, 368336, 4324, 4585) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1513, 3327, 6063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15163) [M::mem_pestat] mean and std.dev: (3929.76, 2789.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (208.71, 85.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1507, 3560, 6237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15697) [M::mem_pestat] mean and std.dev: (4024.06, 2847.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20427) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1541, 3454, 5945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14753) [M::mem_pestat] mean and std.dev: (3931.38, 2743.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19157) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 310.988 CPU sec, 20.133 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4429, 344443, 4202, 4449) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1403, 3343, 5823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14663) [M::mem_pestat] mean and std.dev: (3802.60, 2751.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19083) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 194, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (206.84, 84.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1432, 3481, 6092) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15412) [M::mem_pestat] mean and std.dev: (3935.84, 2774.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20072) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1515, 3497, 6118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15324) [M::mem_pestat] mean and std.dev: (3982.99, 2790.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19927) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 340.513 CPU sec, 22.126 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4531, 370646, 4470, 4586) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1461, 3378, 5982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15024) [M::mem_pestat] mean and std.dev: (3893.07, 2799.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (207.92, 85.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1557, 3476, 6114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15228) [M::mem_pestat] mean and std.dev: (3970.25, 2753.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19785) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1553, 3456, 6130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15284) [M::mem_pestat] mean and std.dev: (3985.37, 2789.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19861) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 307.988 CPU sec, 20.425 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4507, 373140, 4376, 4429) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1510, 3496, 6125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15355) [M::mem_pestat] mean and std.dev: (3967.37, 2797.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19970) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (208.21, 85.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1516, 3526, 6048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15112) [M::mem_pestat] mean and std.dev: (3958.33, 2782.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19644) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1654, 3643, 6252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15448) [M::mem_pestat] mean and std.dev: (4079.22, 2759.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20046) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 301.700 CPU sec, 19.325 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4664, 370441, 4461, 4402) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1433, 3252, 5926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14912) [M::mem_pestat] mean and std.dev: (3839.30, 2767.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (207.62, 85.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1609, 3472, 5946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14620) [M::mem_pestat] mean and std.dev: (3921.36, 2721.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18957) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1538, 3579, 6132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15320) [M::mem_pestat] mean and std.dev: (3998.28, 2772.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19914) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 300.941 CPU sec, 19.317 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4474, 372192, 4490, 4542) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1413, 3327, 5922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14940) [M::mem_pestat] mean and std.dev: (3843.33, 2793.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 194, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (206.96, 84.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1524, 3508, 6261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15735) [M::mem_pestat] mean and std.dev: (3998.91, 2805.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20472) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1533, 3393, 5976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14862) [M::mem_pestat] mean and std.dev: (3929.72, 2745.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19305) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 301.101 CPU sec, 19.165 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4579, 373103, 4369, 4478) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1459, 3383, 5986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15040) [M::mem_pestat] mean and std.dev: (3888.03, 2776.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 194, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (206.46, 84.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1546, 3493, 6137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15319) [M::mem_pestat] mean and std.dev: (3984.85, 2798.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19910) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1572, 3456, 6047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14997) [M::mem_pestat] mean and std.dev: (3967.67, 2754.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 296.349 CPU sec, 18.887 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4564, 372727, 4384, 4539) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1452, 3297, 5965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14991) [M::mem_pestat] mean and std.dev: (3840.39, 2773.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 193, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (205.92, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1537, 3535, 5969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14833) [M::mem_pestat] mean and std.dev: (3920.95, 2717.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19265) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] (25, 50, 75) percentile: (1466, 3497, 6054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15230) [M::mem_pestat] mean and std.dev: (3955.12, 2788.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19818) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 334.926 CPU sec, 21.388 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4655, 362814, 4475, 4610) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1396, 3351, 5940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15028) [M::mem_pestat] mean and std.dev: (3848.84, 2784.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (208.91, 85.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1456, 3346, 6052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15244) [M::mem_pestat] mean and std.dev: (3924.23, 2800.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19840) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1574, 3527, 6187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15413) [M::mem_pestat] mean and std.dev: (4006.85, 2799.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20026) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 319.103 CPU sec, 20.361 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4459, 366920, 4423, 4566) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1421, 3301, 5913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14897) [M::mem_pestat] mean and std.dev: (3838.71, 2782.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 197, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (209.32, 85.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1496, 3399, 6032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15104) [M::mem_pestat] mean and std.dev: (3921.28, 2790.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19640) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1564, 3474, 6108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15196) [M::mem_pestat] mean and std.dev: (4013.31, 2796.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19740) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 310.767 CPU sec, 19.835 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4512, 367981, 4444, 4677) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1395, 3326, 5819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14667) [M::mem_pestat] mean and std.dev: (3793.49, 2730.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19091) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 197, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514) [M::mem_pestat] mean and std.dev: (209.13, 85.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1551, 3454, 6090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15168) [M::mem_pestat] mean and std.dev: (3951.86, 2767.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19707) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1609, 3573, 6101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15085) [M::mem_pestat] mean and std.dev: (4017.97, 2763.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19577) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 307.153 CPU sec, 19.789 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4673, 368897, 4393, 4676) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1498, 3465, 6001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15007) [M::mem_pestat] mean and std.dev: (3932.43, 2763.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 197, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (209.20, 85.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1573, 3585, 6242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15580) [M::mem_pestat] mean and std.dev: (4028.70, 2800.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20249) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1569, 3550, 5997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14853) [M::mem_pestat] mean and std.dev: (3954.81, 2731.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19281) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 297.617 CPU sec, 18.875 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4585, 367102, 4473, 4598) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1464, 3376, 5995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15057) [M::mem_pestat] mean and std.dev: (3880.87, 2780.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (209.09, 85.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1552, 3317, 6024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14968) [M::mem_pestat] mean and std.dev: (3932.23, 2764.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19440) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1500, 3322, 6034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15102) [M::mem_pestat] mean and std.dev: (3908.26, 2766.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19636) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 306.304 CPU sec, 19.667 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4701, 369703, 4514, 4524) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1360, 3352, 6137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15691) [M::mem_pestat] mean and std.dev: (3883.28, 2827.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 197, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (209.34, 86.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1455, 3396, 5951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14943) [M::mem_pestat] mean and std.dev: (3873.98, 2767.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19439) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1444, 3355, 6069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15319) [M::mem_pestat] mean and std.dev: (3915.47, 2805.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19944) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 298.092 CPU sec, 19.022 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4761, 369820, 4427, 4603) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1542, 3482, 5978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14850) [M::mem_pestat] mean and std.dev: (3949.15, 2774.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19286) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 196, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (208.90, 86.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1592, 3500, 6185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15371) [M::mem_pestat] mean and std.dev: (4003.63, 2778.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19964) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1571, 3468, 6099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15155) [M::mem_pestat] mean and std.dev: (3994.22, 2765.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19683) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 299.076 CPU sec, 19.251 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4486, 368107, 4299, 4483) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1488, 3505, 5983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14973) [M::mem_pestat] mean and std.dev: (3947.06, 2786.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (207.34, 84.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1552, 3449, 6074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15118) [M::mem_pestat] mean and std.dev: (3973.42, 2782.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19640) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1476, 3291, 5916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14796) [M::mem_pestat] mean and std.dev: (3866.31, 2748.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19236) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 309.799 CPU sec, 19.749 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4652, 371108, 4402, 4559) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1394, 3417, 6001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15215) [M::mem_pestat] mean and std.dev: (3867.29, 2783.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (207.87, 85.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1547, 3446, 6108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15230) [M::mem_pestat] mean and std.dev: (3927.85, 2744.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19791) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1532, 3466, 6059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15113) [M::mem_pestat] mean and std.dev: (3958.32, 2782.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19640) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 293.848 CPU sec, 18.716 real sec [M::process] read 1333334 sequences (200000100 bp)... [M::process] read 1333334 sequences (200000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4518, 371143, 4421, 4484) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1451, 3406, 5975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15023) [M::mem_pestat] mean and std.dev: (3895.35, 2789.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (207.62, 85.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1534, 3530, 6179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15469) [M::mem_pestat] mean and std.dev: (3989.03, 2797.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20114) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1619, 3413, 6228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15446) [M::mem_pestat] mean and std.dev: (4006.02, 2788.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20055) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1333334 reads in 300.656 CPU sec, 19.359 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4674, 370374, 4502, 4566) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1400, 3271, 5933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14999) [M::mem_pestat] mean and std.dev: (3830.51, 2778.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 195, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (207.77, 85.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1505, 3530, 6121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15353) [M::mem_pestat] mean and std.dev: (3987.80, 2808.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19969) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1544, 3381, 6028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14996) [M::mem_pestat] mean and std.dev: (3957.24, 2770.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19480) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 320.954 CPU sec, 20.381 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4697, 369785, 4427, 4479) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1474, 3375, 5990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15022) [M::mem_pestat] mean and std.dev: (3915.67, 2781.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 194, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (206.80, 84.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1548, 3430, 6081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15147) [M::mem_pestat] mean and std.dev: (3956.86, 2767.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19680) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1544, 3488, 6054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15074) [M::mem_pestat] mean and std.dev: (3977.53, 2758.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19584) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1333334 sequences (200000100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 329.556 CPU sec, 21.069 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4581, 372457, 4289, 4433) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1531, 3453, 6083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15187) [M::mem_pestat] mean and std.dev: (3944.17, 2771.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 194, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (206.79, 84.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1529, 3438, 6191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15515) [M::mem_pestat] mean and std.dev: (3995.82, 2830.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20177) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1676, 3593, 6058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14822) [M::mem_pestat] mean and std.dev: (4017.26, 2721.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19204) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1013674 sequences (152051100 bp)... [M::mem_process_seqs] Processed 1333334 reads in 305.213 CPU sec, 19.473 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3448, 284657, 3329, 3449) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1547, 3449, 6093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15185) [M::mem_pestat] mean and std.dev: (3981.54, 2819.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 193, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (206.00, 84.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1481, 3348, 5931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14831) [M::mem_pestat] mean and std.dev: (3852.32, 2732.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19281) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1569, 3530, 6119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15219) [M::mem_pestat] mean and std.dev: (4006.40, 2779.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19769) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1013674 reads in 228.137 CPU sec, 14.748 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -SP5M -t 20 /oak/stanford/groups/akundaje/marinovg/genomes/Ciliates/Tetrahymena_thermophila_SB210-E-JCVI_TeTherMIC_3/bwa-indexes/Tetrahymena_thermophila_SB210-E-JCVI_TeTherMIC_3.fa /oak/stanford/groups/akundaje/marinovg/papers/2022_Tetrahymena-Hi-C/2022-05-24-figures/juicer-Cell_24_hpm_Hi-C_rep2-GSM4161198-MIC/splits/Reads_R1.fastq.gz /oak/stanford/groups/akundaje/marinovg/papers/2022_Tetrahymena-Hi-C/2022-05-24-figures/juicer-Cell_24_hpm_Hi-C_rep2-GSM4161198-MIC/splits/Reads_R2.fastq.gz [main] Real time: 3579.245 sec; CPU: 55426.291 sec Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m Picked up _JAVA_OPTIONS: -Xmx16384m