# hmmscan :: search sequence(s) against a profile database # HMMER 3.0 (March 2010); http://hmmer.org/ # Copyright (C) 2010 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query sequence file: H1.3.fa # target HMM database: /N/dc2/projects/marinovg/genomes/PFam/PFam27.0/Pfam-A.hmm # output directed to file: H1.3.PFam-27-A # per-seq hits tabular output: H1.3.hmmscan.tblout # number of worker threads: 16 # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: histone_H1.3_[Homo_sapiens]gi|4885377|ref|NP_005311.1 [L=221] Scores for complete sequence (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Model Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3e-22 78.5 2.3 3e-22 78.5 1.6 1.8 2 Linker_histone linker histone H1 and H5 family Domain annotation for each model (and alignments): >> Linker_histone linker histone H1 and H5 family # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 78.5 1.6 2e-26 3e-22 3 77 .] 39 110 .. 37 110 .. 0.95 2 ? -2.6 0.2 0.39 5.7e+03 39 39 .. 160 160 .. 136 184 .. 0.59 Alignments for each domain: == domain 1 score: 78.5 bits; conditional E-value: 2e-26 --HHHHHHHHCCCCCCCTSEEHHHHHHHHHC.CTCCCCCSTTHHHHHHHHHHHHHH CS Linker_histone 3 ppyseMiveaikalkerkGsSvqaikKyiea.nykveinasnlasllklaLkkgvk 57 pp+se+i++a++a+ker+G S +a+kK ++a y+ve+n+ s++kl Lk++v+ histone_H1.3_[Homo_sapiens]gi|4885377|ref|NP_005311.1 39 PPVSELITKAVAASKERSGVSLAALKKALAAaGYDVEKNN----SRIKLGLKSLVS 90 9****************************99689988888....************ PP CTSEE-TT..TTTSEEEESS CS Linker_histone 58 sGeLvqvkgtGasGsfklak 77 +G+Lvq+kgtGasGsfkl+k histone_H1.3_[Homo_sapiens]gi|4885377|ref|NP_005311.1 91 KGTLVQTKGTGASGSFKLNK 110 ******************97 PP == domain 2 score: -2.6 bits; conditional E-value: 0.39 C CS Linker_histone 39 i 39 + histone_H1.3_[Homo_sapiens]gi|4885377|ref|NP_005311.1 160 K 160 2 PP Internal pipeline statistics summary: ------------------------------------- Query sequence(s): 1 (221 residues) Target model(s): 14831 (2610332 nodes) Passed MSV filter: 886 (0.0597397); expected 296.6 (0.02) Passed bias filter: 307 (0.0206999); expected 296.6 (0.02) Passed Vit filter: 45 (0.00303419); expected 14.8 (0.001) Passed Fwd filter: 7 (0.000471984); expected 0.1 (1e-05) Initial search space (Z): 14831 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.21u 0.52s 00:00:00.73 Elapsed: 00:00:01.99 # Mc/sec: 289.89 //