stdin:025999-L467_D6_D10_RNA_CL10-UGAv3-44

Basic Statistics

Measure	Value
Filename	025999-L467_D6_D10_RNA_CL10-UGAv3-44_trimmed
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	253364070
Sequences flagged as poor quality	0
Sequence length	9-449
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CAAGCAGAAGACGGCATACGAGATACACAGAAGTGGCCGATGTTTCGCAT	597775	0.23593519002122124	RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAGATCGCAGTGGCCGATGTTTCGCAT	594802	0.2347617797582743	Illumina PCR Primer Index 2 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATACAGCAGAGTGGCCGATGTTTCGCAT	573116	0.22620255508209985	Illumina PCR Primer Index 2 (96% over 29bp)
CCTTCATCAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAGCAAGAGGT	567210	0.22387152211440242	No Hit
CAAGCAGAAGACGGCATACGAGATCTCAATGAGTGGCCGATGTTTCGCAT	537742	0.21224082799111968	RNA PCR Primer, Index 17 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAGTACAAGGTGGCCGATGTTTCGCAT	531269	0.20968600638598833	RNA PCR Primer, Index 22 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCGCATACAGTGGCCGATGTTTCGCAT	517615	0.20429692339564962	RNA PCR Primer, Index 33 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAGTCACTAGTGGCCGATGTTTCGCAT	499080	0.19698136361639595	RNA PCR Primer, Index 37 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATACCTCCAAGTGGCCGATGTTTCGCAT	495144	0.19542786788987088	Illumina PCR Primer Index 2 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATACAAGCTAGTGGCCGATGTTTCGCAT	491687	0.1940634281727476	Illumina PCR Primer Index 8 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATATCCTGTAGTGGCCGATGTTTCGCAT	489804	0.19332022887065242	RNA PCR Primer, Index 31 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCACTTCGAGTGGCCGATGTTTCGCAT	485027	0.19143479973304817	Illumina PCR Primer Index 5 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATACACGACCGTGGCCGATGTTTCGCAT	467822	0.18464417626382462	RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCATACCAAGTGGCCGATGTTTCGCAT	465631	0.18377941276361717	RNA PCR Primer, Index 22 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCCAGTTCAGTGGCCGATGTTTCGCAT	464967	0.18351733929755706	RNA PCR Primer, Index 23 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAAGGTACAGTGGCCGATGTTTCGCAT	464742	0.18342853428270237	Illumina PCR Primer Index 10 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCAGCGTTAGTGGCCGATGTTTCGCAT	464583	0.1833657787388717	Illumina PCR Primer Index 10 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATACGCTCGAGTGGCCGATGTTTCGCAT	459519	0.18136707387120835	Illumina PCR Primer Index 10 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCTGGCATAGTGGCCGATGTTTCGCAT	458497	0.1809637017592905	RNA PCR Primer, Index 30 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCCGAAGTAGTGGCCGATGTTTCGCAT	451589	0.17823719045877343	Illumina PCR Primer Index 9 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGCGAGTAAGTGGCCGATGTTTCGCAT	451172	0.17807260516457601	RNA PCR Primer, Index 24 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAAACATCGGTGGCCGATGTTTCGCAT	445362	0.17577946233654992	Illumina PCR Primer Index 10 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCCGTGAGAGTGGCCGATGTTTCGCAT	445218	0.1757226271270429	Illumina Paired End PCR Primer 2 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAACCGAGAGTGGCCGATGTTTCGCAT	439230	0.17335922966504289	Illumina PCR Primer Index 10 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAGCAGGAAGTGGCCGATGTTTCGCAT	438071	0.1729017851663024	TruSeq Adapter, Index 25 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCGCTGATCGTGGCCGATGTTTCGCAT	437395	0.17263497543278336	Illumina PCR Primer Index 5 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAGTGGTCAGTGGCCGATGTTTCGCAT	437053	0.1724999918102042	Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAACGTGATGTGGCCGATGTTTCGCAT	435155	0.17175087217378532	RNA PCR Primer, Index 31 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATAGATGTACGTGGCCGATGTTTCGCAT	433014	0.1709058431213234	Illumina PCR Primer Index 2 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATACAGATTCGTGGCCGATGTTTCGCAT	430970	0.1700990988974877	Illumina PCR Primer Index 2 (96% over 27bp)
CAAGCAGAAGACGGCATACGAGATACCACTGTGTGGCCGATGTTTCGCAT	428867	0.16926906802531236	TruSeq Adapter, Index 25 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCTGAGCCAGTGGCCGATGTTTCGCAT	428242	0.1690223874284937	Illumina PCR Primer Index 9 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATTGGTGGTAGTGGCCGATGTTTCGCAT	424441	0.16752217471088146	RNA PCR Primer, Index 36 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATTTCACGCAGTGGCCGATGTTTCGCAT	424378	0.16749730930672213	RNA PCR Primer, Index 13 (96% over 29bp)
GTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGG	416997	0.1645841101305327	Illumina Single End PCR Primer 1 (100% over 29bp)
CAAGCAGAAGACGGCATACGAGATCACCTTACGTGGCCGATGTTTCGCAT	410618	0.16206638928716294	RNA PCR Primer, Index 33 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATATTGAGGAGTGGCCGATGTTTCGCAT	410230	0.16191324997265794	Illumina PCR Primer Index 6 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATACGTATCAGTGGCCGATGTTTCGCAT	409376	0.16157618560516493	Illumina PCR Primer Index 2 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTATCAGCAGTGGCCGATGTTTCGCAT	409027	0.16143843915990141	RNA PCR Primer, Index 19 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCAACCACAGTGGCCGATGTTTCGCAT	408278	0.16114281713267395	RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAACAACCAGTGGCCGATGTTTCGCAT	405561	0.16007044724218394	Illumina PCR Primer Index 12 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCCATCCTCGTGGCCGATGTTTCGCAT	402009	0.15866851207434424	Illumina PCR Primer Index 2 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATACATTGGCGTGGCCGATGTTTCGCAT	401977	0.15865588202778713	Illumina PCR Primer Index 2 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATTAGGATGAGTGGCCGATGTTTCGCAT	397124	0.15674045652960975	RNA PCR Primer, Index 46 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCGACTGGAGTGGCCGATGTTTCGCAT	395345	0.15603830487882517	RNA PCR Primer, Index 33 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATAACTCACCGTGGCCGATGTTTCGCAT	394588	0.15573952533995844	Illumina PCR Primer Index 5 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCCTCCTGAGTGGCCGATGTTTCGCAT	393887	0.15546284838256663	RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGCTAACGAGTGGCCGATGTTTCGCAT	381187	0.15045029865521184	RNA PCR Primer, Index 24 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATCAGATCTGGTGGCCGATGTTTCGCAT	377522	0.14900376363546736	Illumina PCR Primer Index 9 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATATGCCTAAGTGGCCGATGTTTCGCAT	375886	0.14835805250523484	RNA PCR Primer, Index 37 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATTCCGTCTAGTGGCCGATGTTTCGCAT	374766	0.14791600087573586	RNA PCR Primer, Index 40 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCAAGACTAGTGGCCGATGTTTCGCAT	373029	0.14723042616105747	RNA PCR Primer, Index 21 (96% over 27bp)
CAAGCAGAAGACGGCATACGAGATCAAGGAGCGTGGCCGATGTTTCGCAT	370685	0.14630527525074885	RNA PCR Primer, Index 21 (96% over 27bp)
CAAGCAGAAGACGGCATACGAGATCATCAAGTGTGGCCGATGTTTCGCAT	369748	0.14593545169999836	Illumina PCR Primer Index 7 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAGCACCTCGTGGCCGATGTTTCGCAT	362286	0.14299028271846123	TruSeq Adapter, Index 25 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAACGCTTAGTGGCCGATGTTTCGCAT	357475	0.14109143415639006	RNA PCR Primer, Index 31 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGCCACATAGTGGCCGATGTTTCGCAT	356478	0.14069792926834496	RNA PCR Primer, Index 24 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGCTCGGTAGTGGCCGATGTTTCGCAT	350843	0.1384738570074281	Illumina PCR Primer Index 7 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGTCTGTCAGTGGCCGATGTTTCGCAT	347666	0.13721993019767958	Illumina PCR Primer Index 3 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGTGTTCTAGTGGCCGATGTTTCGCAT	343819	0.13570156178814147	RNA PCR Primer, Index 39 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATACTATGCAGTGGCCGATGTTTCGCAT	340455	0.13437382814382481	RNA PCR Primer, Index 44 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGAATCTGAGTGGCCGATGTTTCGCAT	335693	0.1324943193405442	RNA PCR Primer, Index 39 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGACTAGTAGTGGCCGATGTTTCGCAT	331981	0.13102923393991894	Illumina PCR Primer Index 3 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGTCGTAGAGTGGCCGATGTTTCGCAT	329374	0.13000027983446902	RNA PCR Primer, Index 41 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATCTGTAGCCGTGGCCGATGTTTCGCAT	328599	0.12969439589441392	RNA PCR Primer, Index 17 (96% over 29bp)
TCGCCAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGGGATAGGC	328293	0.12957362107421153	Illumina Single End PCR Primer 1 (100% over 32bp)
CAAGCAGAAGACGGCATACGAGATCTAAGGTCGTGGCCGATGTTTCGCAT	327193	0.1291394632238107	Illumina PCR Primer Index 9 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCGACACACGTGGCCGATGTTTCGCAT	321901	0.1270507692744279	RNA PCR Primer, Index 21 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATAGAGTCAAGTGGCCGATGTTTCGCAT	315170	0.12439411791892985	RNA PCR Primer, Index 32 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGAGCTGAAGTGGCCGATGTTTCGCAT	309249	0.12205716461690878	Illumina PCR Primer Index 7 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATAAGACGGAGTGGCCGATGTTTCGCAT	302900	0.11955128444218628	RNA PCR Primer, Index 27 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTCTTCACAGTGGCCGATGTTTCGCAT	298195	0.11769427290933557	RNA PCR Primer, Index 36 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAAGGACACGTGGCCGATGTTTCGCAT	297527	0.1174306206874558	Illumina PCR Primer Index 10 (96% over 27bp)
CAAGCAGAAGACGGCATACGAGATGGAGAACAGTGGCCGATGTTTCGCAT	296021	0.11683621912136159	Illumina PCR Primer Index 11 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCCGACAACGTGGCCGATGTTTCGCAT	294076	0.11606854910406199	Illumina PCR Primer Index 9 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCCTAATCCGTGGCCGATGTTTCGCAT	291523	0.11506090820217721	RNA PCR Primer, Index 22 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGTACGCAAGTGGCCGATGTTTCGCAT	291240	0.11494921122793772	RNA PCR Primer, Index 16 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATTGGCTTCAGTGGCCGATGTTTCGCAT	288528	0.11387881478222227	RNA PCR Primer, Index 15 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTGAAGAGAGTGGCCGATGTTTCGCAT	286577	0.11310877663119322	Illumina PCR Primer Index 8 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGGTGCGAAGTGGCCGATGTTTCGCAT	280375	0.11066091573284248	Illumina PCR Primer Index 1 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGATAGACAGTGGCCGATGTTTCGCAT	274069	0.10817200718318111	RNA PCR Primer, Index 24 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGAACAGGCGTGGCCGATGTTTCGCAT	272214	0.10743985917182337	Illumina PCR Primer Index 7 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTGGAACAAGTGGCCGATGTTTCGCAT	269688	0.10644287487172116	RNA PCR Primer, Index 27 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAATGTTGCGTGGCCGATGTTTCGCAT	268059	0.10579992656417304	Illumina PCR Primer Index 6 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCAATGGAAGTGGCCGATGTTTCGCAT	268015	0.10578256025015702	Illumina PCR Primer Index 5 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCGAACTTAGTGGCCGATGTTTCGCAT	267999	0.10577624522687845	RNA PCR Primer, Index 14 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATGACAGTGCGTGGCCGATGTTTCGCAT	265418	0.1047575530342562	Illumina PCR Primer Index 12 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATATCATTCCGTGGCCGATGTTTCGCAT	260106	0.10266096530577522	TruSeq Adapter, Index 25 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATGATGAATCGTGGCCGATGTTTCGCAT	259589	0.10245691111608682	RNA PCR Primer, Index 43 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATATTGGCTCGTGGCCGATGTTTCGCAT	259523	0.10243086164506278	Illumina PCR Primer Index 6 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATAAGAGATCGTGGCCGATGTTTCGCAT	255004	0.10064726225782526	RNA PCR Primer, Index 27 (96% over 28bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content