stdin:025999-L458_D6_D10_RNA_CL01-UGAv3-127

Basic Statistics

Measure	Value
Filename	025999-L458_D6_D10_RNA_CL01-UGAv3-127_trimmed
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	253796988
Sequences flagged as poor quality	0
Sequence length	8-401
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CACGTCTCGTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGGGATA	824545	0.3248836822287268	Illumina RNA PCR Primer (96% over 31bp)
GTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGGGATA	698945	0.27539530926190503	Illumina Single End PCR Primer 1 (100% over 29bp)
GTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGGGATAGGC	666085	0.2624479530860311	Illumina Single End PCR Primer 1 (100% over 29bp)
CAAGCAGAAGACGGCATACGAGATACAGCAGAGTGGCCGATGTTTCGCAT	662651	0.2610949031436102	Illumina PCR Primer Index 2 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATACACAGAAGTGGCCGATGTTTCGCAT	648988	0.255711466520635	RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAGATCGCAGTGGCCGATGTTTCGCAT	603334	0.2377230733723286	Illumina PCR Primer Index 2 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATACCTCCAAGTGGCCGATGTTTCGCAT	589762	0.23237549217881184	Illumina PCR Primer Index 2 (96% over 29bp)
CCTTCATCAGGGTTTGCTGAAGATGGCGGTATATAGGCTGAGCAAGAGGT	576327	0.2270818911373369	No Hit
CAAGCAGAAGACGGCATACGAGATAAACATCGGTGGCCGATGTTTCGCAT	575529	0.2267674666020859	Illumina PCR Primer Index 10 (96% over 28bp)
CACGTCTCGTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGG	550767	0.21701084963230535	Illumina RNA PCR Primer (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATACACGACCGTGGCCGATGTTTCGCAT	550243	0.216804385401138	RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCACTTCGAGTGGCCGATGTTTCGCAT	546345	0.21526851217004989	Illumina PCR Primer Index 5 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCGACTGGAGTGGCCGATGTTTCGCAT	532732	0.20990477633249138	RNA PCR Primer, Index 33 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATCAACCACAGTGGCCGATGTTTCGCAT	531791	0.20953400755094856	RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCAAGACTAGTGGCCGATGTTTCGCAT	528485	0.2082313916191945	RNA PCR Primer, Index 21 (96% over 27bp)
CAAGCAGAAGACGGCATACGAGATAGTCACTAGTGGCCGATGTTTCGCAT	518990	0.20449021246855772	RNA PCR Primer, Index 37 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTATCAGCAGTGGCCGATGTTTCGCAT	500533	0.19721786453982662	RNA PCR Primer, Index 19 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATACAAGCTAGTGGCCGATGTTTCGCAT	499082	0.19664614774703315	Illumina PCR Primer Index 8 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAACTCACCGTGGCCGATGTTTCGCAT	480838	0.18945772516417728	Illumina PCR Primer Index 5 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAGCAGGAAGTGGCCGATGTTTCGCAT	458976	0.18084375374856695	TruSeq Adapter, Index 25 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAACCGAGAGTGGCCGATGTTTCGCAT	455016	0.17928345154356204	Illumina PCR Primer Index 10 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCCGAAGTAGTGGCCGATGTTTCGCAT	453525	0.1786959741224352	Illumina PCR Primer Index 9 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCAGCGTTAGTGGCCGATGTTTCGCAT	449149	0.17697176138276313	Illumina PCR Primer Index 10 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGTCTGTCAGTGGCCGATGTTTCGCAT	446166	0.17579641252480113	Illumina PCR Primer Index 3 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGACTAGTAGTGGCCGATGTTTCGCAT	443159	0.17461160728983907	Illumina PCR Primer Index 3 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCCTCCTGAGTGGCCGATGTTTCGCAT	439069	0.1730000830427507	RNA PCR Primer, Index 23 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCGCATACAGTGGCCGATGTTTCGCAT	438153	0.1726391646539162	RNA PCR Primer, Index 33 (96% over 29bp)
GTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGGGATAG	430845	0.16975969785740722	Illumina Single End PCR Primer 1 (100% over 29bp)
CAAGCAGAAGACGGCATACGAGATAGTACAAGGTGGCCGATGTTTCGCAT	430615	0.16966907424449024	RNA PCR Primer, Index 22 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATATTGGCTCGTGGCCGATGTTTCGCAT	427957	0.16862178049173696	Illumina PCR Primer Index 6 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATAAGGTACAGTGGCCGATGTTTCGCAT	427548	0.16846062806702813	Illumina PCR Primer Index 10 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATATTGAGGAGTGGCCGATGTTTCGCAT	426950	0.16822500667344406	Illumina PCR Primer Index 6 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAACGTGATGTGGCCGATGTTTCGCAT	421808	0.1661989779011877	RNA PCR Primer, Index 31 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATACGTATCAGTGGCCGATGTTTCGCAT	420331	0.16561701669997753	Illumina PCR Primer Index 2 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATAGTGGTCAGTGGCCGATGTTTCGCAT	418503	0.16489675598514195	Illumina PCR Primer Index 6 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATTTCACGCAGTGGCCGATGTTTCGCAT	417862	0.1646441919160995	RNA PCR Primer, Index 13 (96% over 29bp)
CACGTCTCGTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAGGCACACAGGGGATA	416297	0.1640275573325559	Illumina RNA PCR Primer (96% over 31bp)
CACGTCTCGTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGGGATAGGC	416249	0.16400864457855585	Illumina RNA PCR Primer (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATGATAGACAGTGGCCGATGTTTCGCAT	412261	0.16243730993371758	RNA PCR Primer, Index 24 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCGAACTTAGTGGCCGATGTTTCGCAT	411940	0.16231083089134218	RNA PCR Primer, Index 14 (96% over 30bp)
GTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAGGCACACAGGGGATAGGC	408964	0.1611382401433385	Illumina Single End PCR Primer 1 (100% over 29bp)
CAAGCAGAAGACGGCATACGAGATAATGTTGCGTGGCCGATGTTTCGCAT	408850	0.16109332235258836	Illumina PCR Primer Index 6 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGAGCTGAAGTGGCCGATGTTTCGCAT	408402	0.1609168033152545	Illumina PCR Primer Index 7 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATAAGACGGAGTGGCCGATGTTTCGCAT	408357	0.16089907260837943	RNA PCR Primer, Index 27 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCGCTGATCGTGGCCGATGTTTCGCAT	407991	0.16075486285912896	Illumina PCR Primer Index 5 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCATACCAAGTGGCCGATGTTTCGCAT	405940	0.15994673664133477	RNA PCR Primer, Index 22 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGCTCGGTAGTGGCCGATGTTTCGCAT	404956	0.15955902518433354	Illumina PCR Primer Index 7 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCCGTGAGAGTGGCCGATGTTTCGCAT	404152	0.15924223655483255	Illumina Paired End PCR Primer 2 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGTCGTAGAGTGGCCGATGTTTCGCAT	401746	0.15829423476057958	RNA PCR Primer, Index 41 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATTGGCTTCAGTGGCCGATGTTTCGCAT	395848	0.1559703301128223	RNA PCR Primer, Index 15 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTGGAACAAGTGGCCGATGTTTCGCAT	394476	0.15542974056098727	RNA PCR Primer, Index 27 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAGAGTCAAGTGGCCGATGTTTCGCAT	390185	0.15373901915652363	RNA PCR Primer, Index 32 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGTACGCAAGTGGCCGATGTTTCGCAT	389723	0.15355698389927305	RNA PCR Primer, Index 16 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATACCACTGTGTGGCCGATGTTTCGCAT	386076	0.1521200086109769	TruSeq Adapter, Index 25 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATATGCCTAAGTGGCCGATGTTTCGCAT	381794	0.1504328333478883	RNA PCR Primer, Index 37 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCAATGGAAGTGGCCGATGTTTCGCAT	381712	0.15040052405980484	Illumina PCR Primer Index 5 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCTGTAGCCGTGGCCGATGTTTCGCAT	380152	0.14978585955480292	RNA PCR Primer, Index 17 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATCTGAGCCAGTGGCCGATGTTTCGCAT	378136	0.14899152388680043	Illumina PCR Primer Index 9 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATATCCTGTAGTGGCCGATGTTTCGCAT	376396	0.14830593655429827	RNA PCR Primer, Index 31 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATCATCAAGTGTGGCCGATGTTTCGCAT	374692	0.14763453378729616	Illumina PCR Primer Index 7 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCTGGCATAGTGGCCGATGTTTCGCAT	372881	0.14692097133950227	RNA PCR Primer, Index 30 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCTCAATGAGTGGCCGATGTTTCGCAT	369880	0.1457385301987902	RNA PCR Primer, Index 17 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATACTATGCAGTGGCCGATGTTTCGCAT	369121	0.14543947227616427	RNA PCR Primer, Index 44 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATACGCTCGAGTGGCCGATGTTTCGCAT	365432	0.14398594832811806	Illumina PCR Primer Index 10 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAACGCTTAGTGGCCGATGTTTCGCAT	360509	0.14204620899598697	RNA PCR Primer, Index 31 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCCATCCTCGTGGCCGATGTTTCGCAT	357299	0.14078141857223303	Illumina PCR Primer Index 2 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGATGAATCGTGGCCGATGTTTCGCAT	357246	0.14076053573969127	RNA PCR Primer, Index 43 (96% over 28bp)
GTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAGGCACACAGGGGATA	348898	0.1374712926065143	Illumina Single End PCR Primer 1 (100% over 29bp)
CAAGCAGAAGACGGCATACGAGATGAGTTAGCGTGGCCGATGTTTCGCAT	348465	0.13730068380480542	RNA PCR Primer, Index 29 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAACAACCAGTGGCCGATGTTTCGCAT	344032	0.1355540121697583	Illumina PCR Primer Index 12 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATATCATTCCGTGGCCGATGTTTCGCAT	342857	0.13509104371246516	TruSeq Adapter, Index 25 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATTCTTCACAGTGGCCGATGTTTCGCAT	340870	0.1343081345000044	RNA PCR Primer, Index 36 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCAGATCTGGTGGCCGATGTTTCGCAT	337237	0.1328766754316249	Illumina PCR Primer Index 9 (96% over 30bp)
CAAGCAGAAGACGGCATACGAGATAGCACCTCGTGGCCGATGTTTCGCAT	335807	0.13231323296870645	TruSeq Adapter, Index 25 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATACAGATTCGTGGCCGATGTTTCGCAT	332821	0.13113670206361944	Illumina PCR Primer Index 2 (96% over 27bp)
CAAGCAGAAGACGGCATACGAGATCCTAATCCGTGGCCGATGTTTCGCAT	330015	0.13003109398603266	RNA PCR Primer, Index 22 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGTGTTCTAGTGGCCGATGTTTCGCAT	327949	0.12921705753261342	RNA PCR Primer, Index 39 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGCTAACGAGTGGCCGATGTTTCGCAT	326544	0.12866346546240337	RNA PCR Primer, Index 24 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATAAGGACACGTGGCCGATGTTTCGCAT	321100	0.12651844394622996	Illumina PCR Primer Index 10 (96% over 27bp)
CAAGCAGAAGACGGCATACGAGATAGATGTACGTGGCCGATGTTTCGCAT	316158	0.12457121831564054	Illumina PCR Primer Index 2 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATTAGGATGAGTGGCCGATGTTTCGCAT	314771	0.12402471852818048	RNA PCR Primer, Index 46 (96% over 28bp)
CACGTCTCGTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGGGATAG	313655	0.1235849969976791	Illumina RNA PCR Primer (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATGCCACATAGTGGCCGATGTTTCGCAT	311655	0.12279696558100998	RNA PCR Primer, Index 24 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATGGAGAACAGTGGCCGATGTTTCGCAT	310103	0.12218545320167472	Illumina PCR Primer Index 11 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGAATCTGAGTGGCCGATGTTTCGCAT	309987	0.1221397473795079	RNA PCR Primer, Index 39 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATGCGAGTAAGTGGCCGATGTTTCGCAT	305519	0.12037928519466905	RNA PCR Primer, Index 24 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCCAGTTCAGTGGCCGATGTTTCGCAT	300377	0.1183532564224127	RNA PCR Primer, Index 23 (96% over 29bp)
CAAGCAGAAGACGGCATACGAGATAAGAGATCGTGGCCGATGTTTCGCAT	295078	0.11626536718394782	RNA PCR Primer, Index 27 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATACATTGGCGTGGCCGATGTTTCGCAT	287252	0.11318180025052149	Illumina PCR Primer Index 2 (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATGGTGCGAAGTGGCCGATGTTTCGCAT	286741	0.11298045822356252	Illumina PCR Primer Index 1 (96% over 28bp)
CAAGCAGAAGACGGCATACGAGATCGACACACGTGGCCGATGTTTCGCAT	273519	0.10777078252796288	RNA PCR Primer, Index 21 (96% over 30bp)
CACGTCTCGTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAGGCACACAGGGGATAGGC	271207	0.10685981821029335	Illumina RNA PCR Primer (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATTGGTGGTAGTGGCCGATGTTTCGCAT	260275	0.10255243848677983	RNA PCR Primer, Index 36 (96% over 30bp)
GCTGCCGACGACACGTCTCGTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGA	259312	0.10217300135965364	Illumina RNA PCR Primer (96% over 31bp)
CAAGCAGAAGACGGCATACGAGATGACAGTGCGTGGCCGATGTTTCGCAT	259306	0.10217063726540364	Illumina PCR Primer Index 12 (96% over 28bp)
GTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAAGGCACACAGGG	255581	0.10070292875185737	Illumina Single End PCR Primer 1 (100% over 29bp)
CACGTCTCGTGTAGATCTCGGTGGTCGCCGTATCATTAGCTGAGACTGCCAGGCACACAGGG	255424	0.10064106828564885	Illumina RNA PCR Primer (96% over 31bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content