LOCUS       8_08              9037 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(8054..8914)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2707..3567
                     /note="pLannotate"
                     /label="AmpR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(3817..4611)
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             7010..7804
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      complement(1996..2584)
                     /note="pLannotate"
                     /label="ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(4960..5262)
                     /note="pLannotate"
                     /label="ccdB"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             6359..6661
                     /note="pLannotate"
                     /label="ccdB"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(8915..8967)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="73.61111111111111"
                     /fragment="True"
                     /other="promoter"
     promoter        2654..2706
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="73.61111111111111"
                     /fragment="True"
                     /other="promoter"
     promoter        complement(3660..3719)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="58.82352941176471"
                     /fragment="True"
                     /other="promoter"
     promoter        7902..7961
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="58.82352941176471"
                     /fragment="True"
                     /other="promoter"
     promoter        complement(1642..1672)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="addgene"
                     /identity="96.875"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="addgene"
                     /identity="96.875"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(3740..3796)
                     /note="pLannotate"
                     /label="bom"
                     /database="addgene"
                     /identity="98.246"
                     /match_length="40.42553191489361"
                     /fragment="True"
                     /other="misc feature"
     misc_feature    7825..7881
                     /note="pLannotate"
                     /label="bom"
                     /database="addgene"
                     /identity="98.246"
                     /match_length="40.42553191489361"
                     /fragment="True"
                     /other="misc feature"
     protein_bind    complement(947..969)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="95.83333333333334"
                     /fragment="False"
                     /other="protein bind"
     protein_bind    1616..1638
                     /note="pLannotate"
                     /label="lac operator"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="95.83333333333334"
                     /fragment="False"
                     /other="protein bind"
     protein_bind    complement(877..898)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
     protein_bind    1687..1708
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
     misc_feature    complement(5527..5545)
                     /note="pLannotate"
                     /label="Tn5 ME"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     misc_feature    complement(1116..1134)
                     /note="pLannotate"
                     /label="Tn5 ME"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     misc_feature    1364..1382
                     /note="pLannotate"
                     /label="Tn5 ME"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     misc_feature    5989..6007
                     /note="pLannotate"
                     /label="Tn5 ME"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     promoter        complement(1555..1573)
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        1012..1030
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(6180..6198)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        5423..5441
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             771..864
                     /note="pLannotate"
                     /label="lacI"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="8.679593721144968"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1721..1814)
                     /note="pLannotate"
                     /label="lacI"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="8.679593721144968"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(5273..5420)
                     /note="pLannotate"
                     /label="lacZ"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="4.813008130081301"
                     /fragment="True"
                     /other="CDS"
     CDS             6201..6348
                     /note="pLannotate"
                     /label="lacZ"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="4.813008130081301"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca gaattaaccc
     1021 tcactaaagg gactagtcct gcaggtttaa acgaattcgc ccttaatgat acggcgacca
     1081 ccgagatcta caccgtacca atcgtcggca gcgtcagatg tgtataagag acagggagaa
     1141 tggcgtgagc ccaggaggcg gaggttgcag tgagcggaga tcacaccact gcactccaac
     1201 ctgggtgaca gagtgagatt ccgtctcaaa aaacaacaac aacaacaaca acaacaaaac
     1261 ctcacaaccc tggtctccca gagcttccat attaataaat ctagacaatg tgaaatgcaa
     1321 atccacttaa tatttgatga aaaaaaaaaa aaacactcag atgctgtctc ttatacacat
     1381 ctccgagccc acgagactcg gacgatcatg ggtgaactgg caagtatgca gcgcgctcaa
     1441 gcacgtggat ggattaggag tcgtacgccg atgcgaaaca tcggccactc cagtcgatct
     1501 cgtatgccgt cttctgcttg aagggcgaat tcgtttaaac ctgcaggact agtcccttta
     1561 gtgagggtta attctgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     1621 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     1681 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     1741 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     1801 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     1861 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     1921 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     1981 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2041 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2101 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2161 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2221 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     2281 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     2341 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2401 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     2461 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     2521 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     2581 tcaagagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2641 taagggattt tggtcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     2701 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     2761 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     2821 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     2881 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     2941 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     3001 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     3061 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     3121 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     3181 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     3241 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     3301 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     3361 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     3421 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     3481 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     3541 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     3601 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     3661 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     3721 acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat gcgtaaggag
     3781 aaaataccgc atcaggaaat tgtaagcgtt aataattcag aagaactcgt caagaaggcg
     3841 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     3901 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     3961 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     4021 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     4081 gctcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     4141 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     4201 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     4261 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     4321 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     4381 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt gcagttcatt
     4441 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     4501 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     4561 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     4621 tcctcatcct gtctcttgat cagagcttga tcccctgcgc catcagatcc ttggcggcaa
     4681 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
     4741 caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc
     4801 actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta
     4861 gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg tgaaaaggat
     4921 ctaggtgaag atcctttttg ataatctcat gcctgacatt tatattcccc agaacatcag
     4981 gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat
     5041 aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt catccccgat
     5101 atgcaccacc gggtaaagtt cacgggagac tttatctgac agcagacgtg cactggccag
     5161 ggggatcacc atccgtcgcc ccggcgtgtc aataatatca ctctgtacat ccacaaacag
     5221 acgataacgg ctctctcttt tataggtgta aaccttaaac tgccgtacgt ataggctgcg
     5281 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     5341 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     5401 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattga atttagcggc
     5461 cgcgaattcg cccttaatga tacggcgacc accgagatct acaccgtacc aatcgtcggc
     5521 agcgtcagat gtgtataaga gacaggtttc aaagcaatgt gctattaaag tgttgtggcc
     5581 atgtttctta ctttgttatt cttcccagtg gatctcaaac tattgattct gtttgcaatc
     5641 cagggaaaga tgtgtgctgc tggcctataa cttggtggtg ttgggtctta ggaaatattg
     5701 ccctctttgg tctttagtgt acacatctga gctttggaca tgttatttta aactttttca
     5761 aggatcatat cttagaaaag tgagtgagca tatagctgta caccctttat ttaggttgta
     5821 atcagctctt caaaattaaa tttgttgatt gatgcggtgt cctcagcagg ctcaagtgtt
     5881 gaattctctt agtgttttct aaataatgat aatggaatat ccattatgat ttcttttgca
     5941 ttttaaaaaa tctcactttg tgaacaaaaa aaaaaaaatt gaccttccct gtctcttata
     6001 cacatctccg agcccacgag actcggacga tcatgggtgt ggttgcaagt atgcagcgcg
     6061 ctcaagcacg tggatgaatc tgtagtcgta cgccgatgcg aaacatcggc cacgatctct
     6121 tatctcgtat gccgtcttct gcttgaaggg cgaattcgcg gccgctaaat tcaattcgcc
     6181 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     6241 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     6301 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctat acgtacggca
     6361 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag
     6421 tgatattatt gacacgccgg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct
     6481 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg
     6541 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga
     6601 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata
     6661 aatgtcaggc atgagattat caaaaaggat cttcacctag atccttttca cgtagaaagc
     6721 cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag
     6781 ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct
     6841 agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg
     6901 taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg
     6961 gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     7021 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     7081 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     7141 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     7201 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     7261 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     7321 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     7381 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     7441 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     7501 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     7561 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     7621 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     7681 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     7741 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     7801 ctgaattatt aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg
     7861 tatttcacac cgcatcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     7921 tatttttcta aatacattca aatatgtatc cgctcatgag attatcaaaa aggatcttca
     7981 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8041 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8101 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8161 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8221 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8281 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8341 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8401 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8461 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8521 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8581 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8641 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8701 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8761 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8821 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8881 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8941 gcatttatca gggttattgt ctcatgacca aaatccctta acgtgagttt tcgttccact
     9001 gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//
