LOCUS 5_05 4472 bp DNA circular UNK 01-JAN-1980 DEFINITION . ACCESSION VERSION KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers CDS complement(3489..4349) /note="pLannotate" /label="AmpR" /database="addgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="CDS" CDS 2445..3239 /note="pLannotate" /label="NeoR/KanR" /database="addgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="CDS" rep_origin 1..589 /note="pLannotate" /label="ori" /database="addgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="rep_origin" CDS 1794..2096 /note="pLannotate" /label="ccdB" /database="addgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="CDS" promoter complement(4350..4402) /note="pLannotate" /label="AmpR promoter" /database="addgene" /identity="100.0" /match_length="73.61111111111111" /fragment="True" /other="promoter" promoter 3337..3396 /note="pLannotate" /label="AmpR promoter" /database="addgene" /identity="100.0" /match_length="58.82352941176471" /fragment="True" /other="promoter" promoter 913..943 /note="pLannotate" /label="lac promoter" /database="addgene" /identity="96.875" /match_length="100.0" /fragment="False" /other="promoter" misc_feature 3260..3316 /note="pLannotate" /label="bom" /database="addgene" /identity="98.246" /match_length="40.42553191489361" /fragment="True" /other="misc feature" protein_bind complement(947..969) /note="pLannotate" /label="lac operator" /database="addgene" /identity="100.0" /match_length="95.83333333333334" /fragment="False" /other="protein bind" protein_bind complement(877..898) /note="pLannotate" /label="CAP binding site" /database="addgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="protein bind" misc_feature 1511..1529 /note="pLannotate" /label="Tn5 ME" /database="addgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="misc feature" misc_feature complement(1203..1221) /note="pLannotate" /label="Tn5 ME" /database="addgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="misc feature" promoter 1012..1030 /note="pLannotate" /label="T3 promoter" /database="addgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="promoter" promoter complement(1615..1633) /note="pLannotate" /label="T7 promoter" /database="addgene" /identity="100.0" /match_length="100.0" /fragment="False" /other="promoter" CDS 771..864 /note="pLannotate" /label="lacI" /database="addgene" /identity="100.0" /match_length="8.679593721144968" /fragment="True" /other="CDS" CDS 1636..1783 /note="pLannotate" /label="lacZ" /database="addgene" /identity="100.0" /match_length="4.813008130081301" /fragment="True" /other="CDS" ncRNA complement(1306..1421) /note="pLannotate" /label="LSU rRNA bacteria" /database="infernal" /identity="100.0" /match_length="84.17266187050359" /fragment="True" /other="ncRNA" ORIGIN 1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat 781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca gaattaaccc 1021 tcactaaagg gactagtcct gcaggtttaa acgaattcgc ccttcaagca gaagacggca 1081 tacgagatgc caagacgtgg ccgatgtttc gcatcggcgt acgactgcca agacatccac 1141 gtgcttgagc gcgctgcata cttgatagcg accccatgat cgtccgagtc tcgtgggctc 1201 ggagatgtgt ataagagaca gggcgggttg gttttttttt ttttttgggg atttagaggg 1261 ttctgtgggc aaatttaaag ttgaactaag attctatctt ggacaaccag ctatcaccag 1321 gctcggtagg tttgtcgcct ctacctataa atcttcccac tattttgcta catagacggg 1381 tgtgctcttt tagctgttct taggtagctc gtctggtttc gggggtctta gctttggctc 1441 tccttgcaaa gttatttcta gttaattcat tatgcagaag gtataggggt tagtccttgc 1501 tatattatgc ctgtctctta tacacatctg acgctgccga cgattggtac ggtgtagatc 1561 tcggtggtcg ccgtatcatt aagggcgaat tcgcggccgc taaattcaat tcgccctata 1621 gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg 1681 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg 1741 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctatacgta cggcagttta 1801 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata 1861 ttattgacac gccggggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag 1921 ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga 1981 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca 2041 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt 2101 caggcatgag attatcaaaa aggatcttca cctagatcct tttcacgtag aaagccagtc 2161 cgcagaaacg gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa 2221 acgcaagcgc aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact 2281 gggcggtttt atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg 2341 ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca 2401 ggggatcaag ctctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg 2461 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac 2521 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg 2581 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc 2641 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg 2701 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc 2761 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc 2821 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta 2881 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg 2941 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg 3001 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat 3061 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc 3121 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta 3181 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa 3241 ttattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt 3301 cacaccgcat caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 3361 ttctaaatac attcaaatat gtatccgctc atgagattat caaaaaggat cttcacctag 3421 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 3481 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 3541 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 3601 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 3661 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 3721 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 3781 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 3841 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 3901 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 3961 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 4021 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 4081 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 4141 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 4201 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 4261 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 4321 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 4381 tatcagggtt attgtctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 4441 tcagaccccg tagaaaagat caaaggatct tc //