LOCUS       4_04              4307 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  .
ACCESSION   <unknown id>
VERSION     <unknown id>
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(3324..4184)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2280..3074
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             1629..1931
                     /note="pLannotate"
                     /label="ccdB"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(4185..4237)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="73.61111111111111"
                     /fragment="True"
                     /other="promoter"
     promoter        3172..3231
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="58.82352941176471"
                     /fragment="True"
                     /other="promoter"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="addgene"
                     /identity="96.875"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    3095..3151
                     /note="pLannotate"
                     /label="bom"
                     /database="addgene"
                     /identity="98.246"
                     /match_length="40.42553191489361"
                     /fragment="True"
                     /other="misc feature"
     protein_bind    complement(947..969)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="95.83333333333334"
                     /fragment="False"
                     /other="protein bind"
     protein_bind    complement(877..898)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
     misc_feature    1349..1367
                     /note="pLannotate"
                     /label="Tn5 ME"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     misc_feature    complement(1203..1221)
                     /note="pLannotate"
                     /label="Tn5 ME"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
     promoter        1012..1030
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(1450..1468)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             771..864
                     /note="pLannotate"
                     /label="lacI"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="8.679593721144968"
                     /fragment="True"
                     /other="CDS"
     CDS             1471..1618
                     /note="pLannotate"
                     /label="lacZ"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="4.813008130081301"
                     /fragment="True"
                     /other="CDS"
     rep_origin      3077..3094
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="3.9735099337748347"
                     /fragment="True"
                     /other="rep_origin"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca gaattaaccc
     1021 tcactaaagg gactagtcct gcaggtttaa acgaattcgc ccttcaagca gaagacggca
     1081 tacgagatca ccttacgtgg ccgatgtttc gcatcggcgt acgactagag tcaaatccac
     1141 gtgcttgagc gcgctgcata cttggccaca tacccatgat cgtccgagtc tcgtgggctc
     1201 ggagatgtgt ataagagaca ggggtccatt tgttgactgc aaatcaaggc ctgtggaggg
     1261 agtgctcttt tctcctctcc acctctcaat atctccaagg agcccaggga cacaacaaac
     1321 tcatgttaaa tgtcgattaa tgtacaccct gtctcttata cacatctgac gctgccgacg
     1381 aatagagagg tgtagatctc ggtggtcgcc ttattaaggg cgaattcgcg gccgctaaat
     1441 tcaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg
     1501 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     1561 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctat
     1621 acgtacggca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
     1681 atgtacagag tgatattatt gacacgccgg ggcgacggat ggtgatcccc ctggccagtg
     1741 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
     1801 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
     1861 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
     1921 ggggaatata aatgtcaggc atgagattat caaaaaggat cttcacctag atccttttca
     1981 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
     2041 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
     2101 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
     2161 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
     2221 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca
     2281 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     2341 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     2401 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     2461 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     2521 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     2581 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     2641 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     2701 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     2761 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     2821 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     2881 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     2941 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3001 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3061 acgagttctt ctgaattatt aacgcttaca atttcctgat gcggtatttt ctccttacgc
     3121 atctgtgcgg tatttcacac cgcatcaggt ggcacttttc ggggaaatgt gcgcggaacc
     3181 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag attatcaaaa
     3241 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3301 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     3361 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     3421 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     3481 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     3541 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     3601 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     3661 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     3721 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     3781 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     3841 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     3901 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     3961 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     4021 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     4081 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     4141 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     4201 tattattgaa gcatttatca gggttattgt ctcatgacca aaatccctta acgtgagttt
     4261 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//
