LOCUS       v4_p_sc-eVIP            9788 bp ds-DNA     circular     20-OCT-2022
DEFINITION  .
KEYWORDS    "marker:AmpR"
COMMENT     Expresses barcoded coding genetic variants in mammalian cells.
            <small>[1] Massively parallel phenotyping of variant impact in
            cancer with Perturb-seq reveals a shift in the spectrum of cell
            states induced by somatic mutations Unpublished</small>
FEATURES             Location/Qualifiers
     misc_feature    429..429
                     /label="Not1 site deleted"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     promoter        462..685
                     /label="EF-1α core promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: core promoter for human elongation factor EF-1α"
     intron          686..1624
                     /label="EF-1α intron A"
                     /ApEinfo_revcolor="#a187be"
                     /ApEinfo_fwdcolor="#a187be"
                     /note="note: intron upstream of the start codon of human EF-1α"
     misc_feature    1640..1662
                     /label="twist 5' flanking"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1663..1694
                     /label="Twist 5' flanking during synthesis"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1672..1692
                     /label="amplicon-1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1695..2876
                     /label="TP53 NM000546 ORF library"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: Homo sapiens TP53 note: responds to cellular stresses to regulate the expression of target genes product: human tumor suppressor protein that is often mutated in cancers transl_table: 1 translation: MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD*"
     misc_feature    1778..1798
                     /label="oBL005"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1797..1816
                     /label="amplicon-2"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(1857..1876)
                     /label="oBL006"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1921..1939
                     /label="amplicon-3"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2023..2042
                     /label="amplicon-4"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2057..2076
                     /label="oBL003"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2126..2144
                     /label="amplicon-5"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(2130..2148)
                     /label="oBL004"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2243..2261
                     /label="amplicon-6"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2365..2385
                     /label="amplicon-7"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2483..2502
                     /label="amplicon-8"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2600..2619
                     /label="amplicon-9"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2698..2717
                     /label="amplicon-10"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2715..2734
                     /label="oBL002"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(2791..2810)
                     /label="oBL001"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2796..2815
                     /label="amplicon-11"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2877..2911
                     /label="twist 3' flanking"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2888..2918
                     /label="oBL011'"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2896..2918
                     /label="0BL011"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          2903..2932
                     /label="oBL021 partial"
                     /note="sequence: GTTTAAACCGTGACGATCGATCGATCGATG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2912..2951
                     /label="Inserted sequence"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2933..2951
                     /label="3' UTR random barcodes"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(2967..2986)
                     /label="amplicon-fwd"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     polyA_signal    3003..3227
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: bovine growth hormone polyadenylation signal"
     misc_feature    complement(3004..3023)
                     /label="oBL012"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3286..3403
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: central polypurine tract and central termination sequence of HIV-1"
     misc_feature    3541..3560
                     /label="oBL020"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(3625..3644)
                     /label="oBL019"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(3701..3745)
                     /label="gp41 peptide"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 note: recognized by the 2H10 single-chain llama nanobody product: antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013) transl_table: 1 translation: KNEQELLELDKWASL"
     misc_feature    3930..4163
                     /label="RRE"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm."
     misc_feature    4660..4785
                     /label="HIV-1 Ψ"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: packaging signal of human immunodeficiency virus type 1"
     LTR             4832..5012
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5030..5228)
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: human cytomegalovirus (CMV) immediate early promoter"
     enhancer        5230..5609
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: human cytomegalovirus immediate early enhancer"
     promoter        5875..5979
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     CDS             5980..6048
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             6049..6840
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     rep_origin      7011..7599
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     LTR             7661..7841
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     8351..8367
                     /label="KS primer"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     misc_feature    8370..8958
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: woodchuck hepatitis virus posttranscriptional regulatory element"
     CDS             8476..8487
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: Factor Xa recognition and cleavage site transl_table: 1 translation: IEGR"
     protein_bind    complement(9014..9047)
                     /label="loxP"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: Cre recombinase note: Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)."
     CDS             complement(9072..9674)
                     /label="Puro"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="codon_start: 1 transl_table: 1 translation: MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS*"
     promoter        complement(9686..397)
                     /label="PGK promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: mouse phosphoglycerate kinase 1 promoter"
ORIGIN
        1 cccctgagcc cgcccctgag cccgcccccg gacccacccc ttcccagcct ctgagcccag
       61 aaagcgaagg agcaaagctg ctattggccg ctgccccaaa ggcctacccg cttccattgc
      121 tcagcggtgc tgtccatctg cacgagacta gtgagacgtg ctacttccat ttgtcacgtc
      181 ctgcacgacg cgagctgcgg ggcggggggg aacttcctga ctaggggagg agtagaaggt
      241 ggcgcgaagg ggccaccaaa gaacggagcc ggttggcgcc taccggtgga tgtggaatgt
      301 gtgcgaggcc agaggccact tgtgtagcgc caagtgccca gcggggctgc taaagcgcat
      361 gctccagact gccttgggaa aagcgcctcc cctacccggg ggtaaattta gttcctgttc
      421 cattaacgcg ccgcgtggat aaccgtatta ccgccatgca tgtgcccgtc agtgggcaga
      481 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
      541 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
      601 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
      661 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
      721 ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat
      781 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
      841 agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg
      901 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa
      961 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca
     1021 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc
     1081 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg
     1141 gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc
     1201 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct
     1261 tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg
     1321 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc
     1381 cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgcgctttt ggagtacgtc
     1441 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga
     1501 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga
     1561 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt
     1621 tcaggtgtcg tgagctagcg ccctctagac tcgagcggcc gcCACTGTGC TGGATATCTG
     1681 CAGAATTCGC CACCATGGAG GAGCCGCAGT CAGATCCTAG CGTCGAGCCC CCTCTGAGTC
     1741 AGGAAACATT TTCAGACCTA TGGAAACTAC TTCCTGAAAA CAACGTTCTG TCCCCCTTGC
     1801 CGTCCCAAGC AATGGATGAT TTGATGCTGT CCCCGGACGA TATTGAACAA TGGTTCACTG
     1861 AAGACCCAGG TCCAGATGAA GCTCCCAGAA TGCCAGAGGC TGCTCCCCCC GTGGCCCCTG
     1921 CACCAGCAGC TCCTACACCG GCGGCCCCTG CACCAGCCCC CTCCTGGCCC CTGTCATCTT
     1981 CTGTCCCTTC CCAGAAAACC TACCAGGGCA GCTACGGTTT CCGTCTGGGC TTCTTGCATT
     2041 CTGGGACAGC CAAGTCTGTG ACTTGCACGT ACTCCCCTGC CCTCAACAAG ATGTTTTGCC
     2101 AACTGGCCAA GACCTGCCCT GTGCAGCTGT GGGTTGATTC CACACCCCCG CCCGGCACCC
     2161 GCGTCCGCGC CATGGCCATC TACAAGCAGT CACAGCACAT GACGGAGGTT GTGAGGCGCT
     2221 GCCCCCACCA TGAGCGCTGC TCAGATAGCG ATGGTCTGGC CCCTCCTCAG CATCTTATCC
     2281 GAGTGGAAGG AAATTTGCGT GTGGAGTATT TGGATGACAG AAACACTTTT CGACATAGTG
     2341 TGGTGGTGCC CTATGAGCCG CCTGAGGTTG GCTCTGACTG TACCACCATC CACTACAACT
     2401 ACATGTGTAA CAGTTCCTGC ATGGGCGGCA TGAACCGGAG GCCCATCCTC ACCATCATCA
     2461 CACTGGAAGA CTCCAGTGGT AATCTACTGG GACGGAACAG CTTTGAGGTG CGTGTTTGTG
     2521 CCTGTCCTGG GAGAGACCGG CGCACAGAGG AAGAGAATCT CCGCAAGAAA GGGGAGCCTC
     2581 ACCACGAGCT GCCCCCAGGG AGCACTAAGC GAGCACTGCC CAACAACACC AGCTCCTCTC
     2641 CCCAGCCAAA GAAGAAACCA CTGGATGGAG AATATTTCAC CCTTCAGATC CGTGGGCGTG
     2701 AGCGCTTCGA GATGTTCCGA GAGCTGAATG AGGCCTTGGA ACTCAAGGAT GCCCAGGCTG
     2761 GGAAGGAGCC AGGGGGGAGC AGGGCTCACT CCAGCCACCT GAAGTCCAAA AAGGGTCAGT
     2821 CTACCTCCCG CCATAAAAAA CTCATGTTCA AGACAGAAGG GCCTGACTCA GACTGAggat
     2881 ccgagctcgg taccaagctt aAGTTTAAAC CGTGACGATC GATCGATCGA TGBNSWNSKM
     2941 SVNBSWNMKY Ngtggtgtta attaacctag agggcccgtt taaacccgct gatcagcctc
     3001 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     3061 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     3121 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     3181 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggtct agagcgggcc
     3241 cggtactaac caaactggat ctctgctgtc cctgtaataa acccgaaaat tttgaatttt
     3301 tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct
     3361 ttcccctgca ctgtaccccc caatcccccc ttttctttta aaattgtgga tgaatactgc
     3421 catttgtctg cagaattggc gcacgcagtg ccgatccgtt cactaatcga atggatctgt
     3481 ctctgtctct ctctccacct tcttcttcta ttccttcggg cctgtcgggt cccctcgggg
     3541 ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag
     3601 aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt
     3661 ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta
     3721 attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt
     3781 gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc
     3841 aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa
     3901 tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca
     3961 cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct
     4021 gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt
     4081 gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca
     4141 agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc
     4201 tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata
     4261 attcacttct ccaattgtcc ctcatatctc ctcctccagg tctgaagatc agcggccggc
     4321 cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc
     4381 cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata
     4441 taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg
     4501 tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt ctagctccct
     4561 gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat
     4621 tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct
     4681 ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc
     4741 tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc
     4801 tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag
     4861 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     4921 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     4981 caggctcaga tctggtctaa ccagagagac ccagtacagg caaaacgcgc tgcttatata
     5041 gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca
     5101 ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg
     5161 gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa
     5221 ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc
     5281 ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat
     5341 agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa
     5401 atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt
     5461 cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt
     5521 aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg taattgatta
     5581 ctattaataa ctagtcaata atcaatgtca acgcgtatat ctggcccgta catcgcgaag
     5641 cagcgcaaaa cgcctaaccc taagcagatt cttcatgcaa ttgtcggtca agccttgcct
     5701 tgttgtagct taaattttgc tcgcgcacta ctcagcgacc tccaacacac aagcagggag
     5761 cagatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatagggg
     5821 atcgggagat ctcccgatcc gtcgacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     5881 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5941 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     6001 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     6061 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     6121 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     6181 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     6241 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6301 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6361 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6421 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6481 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6541 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6601 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6661 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6721 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6781 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6841 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6901 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6961 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7021 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7081 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7141 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     7201 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7261 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7321 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7381 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7441 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7501 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7561 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7621 ttacggttcc tggccttttg ctggcctttt gctcacatgc tgctagagat tttccacact
     7681 gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact
     7741 acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca
     7801 gagagctccc aggctcagat ctggtctaac cagagagacc cagtacagtc cggatgcagc
     7861 tctcgggcca tgtgatgaaa tgctaggcgg ctgtcaaacc tccactctaa tacttctctc
     7921 tccgggtcat ccatcccatg caggctcaca gggtgtaaca agcgggtgtt ctctccttca
     7981 ttggcttctt ctaccttctc ttgctcaact ggtactagct tgtagcacca tccaaaggtc
     8041 agtggatatc tgatccctgg ccctggtgtg tagttctgcc aatcagggaa gtagccttgt
     8101 gtgtggtaga tccacagatc aaggatatct tgtcttcgtt gggagtgaat tagcccttcc
     8161 agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta agtcattggt
     8221 cttaaaggta cctgaggtgt gactggaaaa cccacctcct cctcctcttg tgcttctagc
     8281 caggcacaat cagcattggt agctgctgta ttgctacttg tgattgctcc atgtttttct
     8341 aggtctcgat cgaggtcgac ggtatcgatg cggggaggcg gcccaaaggg agatccgact
     8401 cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga
     8461 aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat
     8521 ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg acaacaccac
     8581 ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat
     8641 gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt
     8701 ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg
     8761 ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga
     8821 ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta
     8881 tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt
     8941 gtaatccaga ggttgattat cgataagctt gatatcgaat tgtacctagt ggaaccggaa
     9001 cccttaaaca tgtataactt cgtataatgt atgctatacg aagttattag gtccctcgac
     9061 gttaacgatc cttaactggc accgggcttg cgggtcatgc accaggtgcg cggtccttcg
     9121 ggcacctcga cgtcggcggt gacggtgaag ccgagccgct cgtagaaggg gaggttgcgg
     9181 ggcgcggagg tctccaggaa ggcaggcacc ccggcgcgct cggcagcttc cactccgggg
     9241 agcacgacgg cgctgcccag acccttgccc tggtggtcgg gcgaaacgcc gacggtggcc
     9301 aggaaccacg cgggctcctt gggccggtgc ggcgccagga ggccttccat ctgttgctgc
     9361 gcggccagcc gggaaccgct caactcggcc atgcgcgggc cgatctcggc gaacaccgcc
     9421 cccgcttcga cgctctccgg cgtggtccag accgccacgg cggcgccgtc atcagcgacc
     9481 cacaccttgc cgatgtcgag cccgacgcgc gtgaggaaga gttcttgcag ctcggtgacc
     9541 cgctcgatgt ggcggtccgg atcgacggtg tggcgcgtgg cggggtagtc ggcgaacgcg
     9601 gcggcgaggg tgcgtacggc cctagggaca tcatcgcggg tggcgaggcg caccgtgggc
     9661 ttgtactcgg tcatggtggc ccgagcgaaa ggcccggaga tgaggaagag gagaacagcg
     9721 cggcagacgt gcgcttttga agcgtgcaga atgccgggcc tccggaggac cttcgggcgc
     9781 ccgccccg
//