## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5395-Z0081-CTTGATCATACAGAT/441525-L5395-Z0081-CTTGATCATACAGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5395-Z0081-CTTGATCATACAGAT/441525-L5395-Z0081-CTTGATCATACAGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:51:55 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
999408	999408	209	209260965	176781199	0.844788	146843793	0.701726	311186702	311186702

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2031890	26.239867	26.25	26.25	26.25
1	200657	34.44727	35	34.25	35
2	4068	34.580933	35	34.25	35
3	1179164	34.434213	35	34.25	35
4	8168	32.699116	32.5	31.75	34.25
5	9808	34.721559	35	35	35
6	2746595	32.73465	34.25	31.75	35
7	11706	32.509152	33.75	31	35
8	15958	33.242761	33.75	32.5	35
9	4734836	33.478589	34.25	32.5	35
10	7260	34.221428	35	34.25	35
11	15874	32.458266	33.75	31.75	35
12	5773419	32.873935	35	32.5	35
13	38539	32.776155	35	32.5	35
14	611841	32.868633	35	32.5	35
15	4294357	32.827655	35	32.5	35
16	606050	32.919884	35	32.5	35
17	4887695	32.836981	35	32.5	35
18	130	32.817863	35	32.5	35
19	5301751	32.80201	35	32.5	35
20	289499	32.768285	35	32.5	35
21	306733	32.716678	35	32.25	35
22	3304111	32.542962	35	31.75	35
23	0	32.353347	35	31.75	35
24	34329	32.059257	34.25	31.75	35
25	25240790	31.453223	34.25	31	35
26	0	31.272978	34.25	30.5	35
27	20	30.809841	34.25	30	35
28	205208	30.30559	33.75	29.25	35
29	556716	29.80113	33.75	27.75	35
30	5292234	29.31015	33.75	26	35
31	0	28.833293	33.5	24.75	35
32	9219474	28.339608	32.75	22.75	35
33	0	27.876432	32.5	21.25	35
34	0	27.532829	31.75	20.25	35
35	132332085	27.193599	31.75	19.75	35
36	0	27.019918	31	19.5	35
37	0	26.723703	30	19.25	35
38	0	26.499878	29.5	19	35
39	0	26.260954	28.5	18.75	35
40	0	25.991352	27.5	18.5	35
41	0	25.802552	27.5	18.5	34.25
42	0	25.579961	27	18	34.25
43	0	25.414894	26.25	18	34.25
44	0	25.257	26	17.75	34.25
45	0	25.172206	26	17.75	34.25
46	0	25.089377	25.5	17.5	34.25
47	0	25.084691	25.5	17.5	34.25
48	0	25.10198	25.25	17.75	34.25
49	0	25.057143	25	17.75	34.25
50	0	24.993396	25	17.75	33.75
51	0	24.890127	25	17.75	33.75
52	0	24.83762	25	17.75	33.75
53	0	24.642322	25	17.5	33.75
54	0	24.455979	25	17.25	33.75
55	0	24.215649	25	17	33.5
56	0	24.232474	25	17.25	33.5
57	0	24.149993	25	17.25	33
58	0	24.119938	25	17.5	32.5
59	0	23.982778	24.5	17.5	32.5
60	0	23.830345	24	17.5	31.75
61	0	23.761959	23.75	17.5	31.75
62	0	23.687187	23.5	17.75	31.25
63	0	23.653834	23.75	18	30.5
64	0	23.679308	24.25	18.25	30
65	0	23.703019	24.75	18.5	30
66	0	23.716503	25	18.5	30
67	0	23.713984	25	18.5	30
68	0	23.748877	25	18.5	30
69	0	23.841027	25	19	30
70	0	23.906149	25	19	30
71	0	23.899834	25	19.25	30
72	0	23.872165	25	19.25	30
73	0	23.862074	25	19.5	30
74	0	23.844562	25	19.5	30
75	0	23.673297	25	19	29.75
76	0	23.493014	25	18.5	29.25
77	0	23.202949	25	17.5	30
78	0	22.927752	25	17	30
79	0	22.772451	25	16.5	30
80	0	22.920642	25	16.75	30
81	0	23.008224	25	17	30
82	0	23.108596	25	17.25	30
83	0	23.147874	25	17.5	30
84	0	23.156337	25	17.5	30
85	0	23.186898	25	17.5	30
86	0	23.032547	24.5	17	30
87	0	22.707576	24	16.5	30
88	0	22.636344	23.75	16.5	29.75
89	0	22.657164	23.5	16.5	29.25
90	0	22.662231	23.5	16.666667	29.25
91	0	22.657498	23.5	16.5	29.5
92	0	22.825878	23.5	16.75	30
93	0	23.173399	24.25	17	30
94	0	22.926264	23.5	16.75	30
95	0	22.927744	23.5	16.666667	30
96	0	22.809735	23.5	16.666667	30
97	0	22.626292	23.5	16.25	30
98	0	22.681022	23.5	16.5	29.25
99	0	22.641153	23	16.666667	29.25
100	0	22.196742	22.5	16	29.25
101	0	21.938973	22	15.75	28.5
102	0	21.599111	21.5	15.5	27.75
103	0	21.422593	21	15.5	27.5
104	0	20.869697	20.5	15.25	26.75
105	0	19.902952	19.25	14.25	25
106	0	19.044633	18.5	13.5	24.25
107	0	17.817876	17.5	12.75	22.5
108	0	9.911407	9.333333	6	12.5