## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5393-Z0079-CGCGCGCAGAATGAT/441525-L5393-Z0079-CGCGCGCAGAATGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5393-Z0079-CGCGCGCAGAATGAT/441525-L5393-Z0079-CGCGCGCAGAATGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:50:20 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
999446	999446	179	179816606	148345713	0.824983	121523927	0.675821	262664702	262664702

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2028097	26.235372	26.25	26.25	26.25
1	209100	34.313609	35	34.25	35
2	2839	34.361551	35	34.25	35
3	1101220	33.975224	34.25	32.5	35
4	6405	32.033754	32.5	31	34.25
5	7274	34.783518	35	35	35
6	2725870	32.51732	32.5	31.75	35
7	9304	32.161016	32.5	30.25	35
8	12259	33.368268	33.75	32.5	35
9	4728729	33.208302	34.25	32.5	35
10	5634	33.851674	35	33.5	35
11	12631	32.230061	33.75	31.75	35
12	5636719	32.613969	35	31.75	35
13	32531	32.644839	35	31.75	35
14	560272	32.744291	35	32.25	35
15	4140582	32.674963	35	31.75	35
16	567460	32.806031	35	32.5	35
17	4708734	32.75093	35	32.25	35
18	48	32.709636	35	31.75	35
19	4975185	32.656017	35	31.75	35
20	251011	32.577828	35	31.75	35
21	238438	32.45429	35	31.75	35
22	2848389	32.144811	34.25	31.5	35
23	0	31.719184	34.25	31	35
24	27425	31.10053	34.25	30	35
25	22866243	30.018912	33.75	28.5	35
26	0	29.587937	33.75	27.5	35
27	11	28.80642	33.5	25	35
28	155127	28.004461	32.5	22.25	35
29	435142	27.247979	31.75	19.5	35
30	4202924	26.579137	31.25	18	35
31	0	26.006454	30	17.25	35
32	8507990	25.44062	27.75	16.75	34.25
33	0	24.971583	25.5	16.5	34.25
34	0	24.621431	25	16.5	34.25
35	108813013	24.255436	25	16.5	33.75
36	0	24.131633	25	16.666667	33.75
37	0	23.797651	24	16.5	32.75
38	0	23.537533	23.5	16.5	32.5
39	0	23.282751	23.5	16.5	31.75
40	0	22.927962	23	16	31.75
41	0	22.662642	23	15.75	31
42	0	22.373977	22.5	15.5	30.25
43	0	22.131235	22	15.25	30
44	0	21.95931	22	15.25	30
45	0	21.813975	21.5	15	30
46	0	21.724844	21.5	15	29.25
47	0	21.726454	21.5	15.25	29.25
48	0	21.771455	21.5	15.25	28.75
49	0	21.741754	21.5	15.25	28.5
50	0	21.72781	21.75	15.5	28.5
51	0	21.652209	21.5	15.5	27.75
52	0	21.684939	21.75	15.75	27.5
53	0	21.531927	21.5	15.75	27.5
54	0	21.420764	21.5	15.5	27.5
55	0	21.319523	21	15.5	27.25
56	0	21.442985	21.5	15.75	27.5
57	0	21.499798	21.5	15.75	27.5
58	0	21.672772	22	16	27.5
59	0	21.706037	22	16	27.5
60	0	21.739487	22	16	27.5
61	0	21.953852	22.5	16.25	27.5
62	0	22.142455	23	16.666667	27.5
63	0	22.343393	23	17	28
64	0	22.597633	23.5	17.25	28.5
65	0	22.805811	23.5	17.5	29.25
66	0	23.015299	24	17.75	30
67	0	23.115307	24.5	17.75	30
68	0	23.317882	25	17.75	30
69	0	23.572178	25	18.25	30
70	0	23.715123	25	18.5	30
71	0	23.891563	25	18.75	30
72	0	23.993574	25	19	30
73	0	24.077605	25	19.25	30
74	0	24.210147	25	19.5	30
75	0	24.115531	25	19	30
76	0	24.119009	25	19	30
77	0	24.025585	25	18.5	30
78	0	24.010606	25	18.5	30
79	0	23.929302	25	18.25	30.25
80	0	24.143392	25.25	18.75	30.5
81	0	24.198823	25.5	19	30.25
82	0	24.291028	25.5	19	30.25
83	0	24.288171	25.5	19.25	30.25
84	0	24.178912	25	19	30
85	0	24.127644	25	18.75	30
86	0	24.072567	25	18.75	30
87	0	23.913741	25	18.5	30
88	0	23.809425	25	18.25	30
89	0	23.836065	25	18.25	30
90	0	23.740394	25	18	30
91	0	23.686261	25	18.25	30
92	0	23.487802	25	18	30
93	0	23.755946	25	18	30
94	0	23.573585	24.25	17.75	30
95	0	23.360473	24	17.25	30
96	0	23.008339	23.5	17	30
97	0	22.918008	23.5	17	29.75
98	0	23.008126	23.5	17	30
99	0	22.891997	23.5	17	29.25
100	0	22.695151	22.75	17	29.25
101	0	22.054544	22	15.75	28.5
102	0	21.866249	22	15.5	27.75
103	0	21.595233	21.25	15.5	27.5
104	0	21.093619	20.5	15.25	26.75
105	0	20.27783	19.75	14.75	25
106	0	19.137052	18.5	13.5	24.75
107	0	17.848742	17.75	12.25	22.75
108	0	9.702797	9.333333	6	12.5