## htsjdk.samtools.metrics.StringHeader
# CollectQualityYieldMetricsFlow INCLUDE_BQ_HISTOGRAM=true INPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5274-Z0013-CAGTTGTGCTGTGAT/441525-L5274-Z0013-CAGTTGTGCTGTGAT_sample.sam.gz OUTPUT=/Ddata/Runs/441525/output/441525-20260312_0210/441525-L5274-Z0013-CAGTTGTGCTGTGAT/441525-L5274-Z0013-CAGTTGTGCTGTGAT_FlowQ.metric    INCLUDE_SECONDARY_ALIGNMENTS=false INCLUDE_SUPPLEMENTAL_ALIGNMENTS=false IGNORET0TAG=false FILLINGVALUE=0.0 KEEPBOUNDARYFLOWS=false ASSUME_SORTED=true STOP_AFTER=0 VERBOSITY=INFO QUIET=false VALIDATION_STRINGENCY=STRICT COMPRESSION_LEVEL=5 MAX_RECORDS_IN_RAM=500000 CREATE_INDEX=false CREATE_MD5_FILE=false USE_JDK_DEFLATER=false USE_JDK_INFLATER=false
## htsjdk.samtools.metrics.StringHeader
# Started on: Fri Mar 13 02:51:53 EDT 2026

## METRICS CLASS	picard.analysis.CollectQualityYieldMetricsFlow$QualityYieldMetricsFlow
TOTAL_READS	PF_READS	MEAN_READ_LENGTH_IN_FLOWS	PF_FLOWS	PF_Q20_FLOWS	PCT_PF_Q20_FLOWS	PF_Q30_FLOWS	PCT_PF_Q30_FLOWS	Q20_EQUIVALENT_YIELD	PF_Q20_EQUIVALENT_YIELD
997484	997484	225	224737890	191728167	0.853119	159297211	0.708813	336296422	336296422

## HISTOGRAM	java.lang.Integer
KEY	QUAL_COUNT	MEAN_CYCLE_QUAL	MEDIAN_CYCLE_QUAL	Q25_CYCLE_QUAL	Q75_CYCLE_QUAL
0	2028207	26.242754	26.25	26.25	26.25
1	182861	34.400886	35	34.25	35
2	6032	34.455935	35	34.25	35
3	1109995	33.81072	34.25	32.5	35
4	11374	32.017472	32.5	30	34.25
5	11857	34.721179	35	35	35
6	2719628	33.365668	34.25	32.5	35
7	13364	32.660934	34.25	31	35
8	17169	33.108562	33.75	32.5	35
9	4728825	33.353431	34.25	32.5	35
10	7733	34.281678	35	34.25	35
11	16457	32.718638	33.75	31.75	35
12	5800300	33.193672	35	33	35
13	43104	33.102852	35	32.5	35
14	618031	33.175411	35	33	35
15	4388245	33.107618	35	32.5	35
16	627433	33.194272	35	33	35
17	5065983	33.112836	35	32.5	35
18	110	33.132053	35	32.5	35
19	5613015	33.128239	35	32.5	35
20	297163	33.090769	35	32.5	35
21	381965	33.044859	35	32.5	35
22	3500399	32.953105	35	32.5	35
23	0	32.807738	35	32.5	35
24	37853	32.562758	35	32.25	35
25	27304670	32.181791	34.25	31.75	35
26	0	31.896748	34.25	31.75	35
27	40	31.511217	34.25	31.25	35
28	253028	31.084637	34.25	30.75	35
29	655838	30.637258	34.25	30	35
30	6124116	30.207409	33.75	29.25	35
31	0	29.785165	33.75	27.75	35
32	9967718	29.34426	33.75	25.75	35
33	0	28.949153	33.5	25	35
34	0	28.636867	33.25	23.5	35
35	143205377	28.316759	32.5	22.5	35
36	0	28.155562	32.5	22	35
37	0	27.8478	32.25	21.5	35
38	0	27.594886	31.75	21	35
39	0	27.335547	31.25	20.75	35
40	0	27.033069	30.75	20.25	35
41	0	26.811413	30	19.75	35
42	0	26.549023	30	19.5	35
43	0	26.360821	29.25	19	35
44	0	26.176852	29	19	34.25
45	0	26.03196	28.5	18.5	34.25
46	0	25.880413	28	18.5	34.25
47	0	25.811743	27.5	18.5	34.25
48	0	25.746413	27.5	18.5	34.25
49	0	25.623596	27.5	18.5	34.25
50	0	25.512709	26.75	18.5	34.25
51	0	25.334785	26	18.25	34.25
52	0	25.249215	25.25	18.25	33.75
53	0	25.035472	25	18	33.75
54	0	24.829558	25	17.75	33.75
55	0	24.612012	25	17.75	33.75
56	0	24.641335	25	18	33.75
57	0	24.559071	25	18	33.5
58	0	24.567877	25	18	33.25
59	0	24.454827	25	18	32.5
60	0	24.391375	25	18.25	32.5
61	0	24.388725	25	18.5	32.5
62	0	24.393273	25	18.5	31.75
63	0	24.422589	25	19	31.75
64	0	24.489264	25	19	31.75
65	0	24.593373	25	19.5	31.75
66	0	24.664502	25	19.5	31.75
67	0	24.70239	25	19.5	31.75
68	0	24.764431	25	19.75	31.75
69	0	24.87112	25	20	31.75
70	0	24.93483	25	20.25	31.75
71	0	24.990391	25.25	20.5	31.25
72	0	24.988904	25.5	20.5	31
73	0	25.007361	25.5	20.5	31
74	0	24.993035	25.5	20.5	31
75	0	24.918211	25.5	20.25	31
76	0	24.772979	25.5	20	31
77	0	24.653741	25.5	19.5	31
78	0	24.494126	25.5	19.25	31
79	0	24.439751	26	19	31.25
80	0	24.502853	26	19	31
81	0	24.596634	26	19.5	31.25
82	0	24.670607	26	19.5	31.75
83	0	24.632096	26	19.5	31
84	0	24.617696	26	19.5	31
85	0	24.473146	25.5	19.25	31
86	0	24.306641	25.25	18.75	31
87	0	24.214825	25.25	18.75	31
88	0	24.147309	25.25	18.5	31
89	0	24.163826	25.25	18.5	31
90	0	24.153329	25	18.75	30.5
91	0	24.13009	25	18.5	30.5
92	0	24.040868	25	18.5	30.25
93	0	24.198978	25	18.75	31
94	0	24.068968	25	18.5	30.5
95	0	23.795643	25	18	30.25
96	0	23.522566	24.5	17.5	30
97	0	23.42229	24.25	17.25	30
98	0	23.348049	24	17.25	30
99	0	23.180243	23.5	17.25	30
100	0	22.85786	23.5	17	29.25
101	0	22.367022	22.75	16.25	29.25
102	0	21.917284	22	16	28
103	0	21.700269	21.75	15.75	27.5
104	0	21.301366	21	15.5	27
105	0	20.290347	20	14.75	25.5
106	0	19.37042	19	13.75	25
107	0	18.195568	18	13	23
108	0	10.489778	10	6	12.666667