Bismark report for: S2_PBAT_2_S34_R1_001_val_1.fq.gz and S2_PBAT_2_S34_R2_001_val_2.fq.gz (version: v0.19.0) Bismark was run with Bowtie 2 against the bisulfite genome of /oak/stanford/groups/akundaje/marinovg/genomes/dm6/bismarck_indexes-BT2/ with the specified options: -q --score-min L,0,-0.2 --ignore-quals --no-mixed --no-discordant --dovetail --maxins 1000 Option '--pbat' specified: alignments to original strands (OT, OB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 11983563 Number of paired-end alignments with a unique best hit: 4353540 Mapping efficiency: 36.3% Sequence pairs with no alignments under any condition: 6523202 Sequence pairs did not map uniquely: 1106821 Sequence pairs which were discarded because genomic sequence could not be extracted: 105 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 0 ((converted) top strand) GA/CT/CT: 2178387 (complementary to (converted) top strand) GA/CT/GA: 2175048 (complementary to (converted) bottom strand) CT/GA/GA: 0 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 105865907 Total methylated C's in CpG context: 2005634 Total methylated C's in CHG context: 529760 Total methylated C's in CHH context: 1198093 Total methylated C's in Unknown context: 423 Total unmethylated C's in CpG context: 21728191 Total unmethylated C's in CHG context: 22197249 Total unmethylated C's in CHH context: 58206980 Total unmethylated C's in Unknown context: 13659 C methylated in CpG context: 8.5% C methylated in CHG context: 2.3% C methylated in CHH context: 2.0% C methylated in unknown context (CN or CHN): 3.0% Bismark completed in 0d 2h 23m 2s