SUMMARISING RUN PARAMETERS ========================== Input filename: L79-Saccharomyces_paradoxus-CG_only.end1.36mers.fastq.gz Trimming mode: paired-end Trim Galore version: 0.4.4 Cutadapt version: 1.15 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp All sequences will be trimmed by 1 bp on their 3' end to avoid problems with invalid paired-end alignments with Bowtie 1 Output file will be GZIP compressed This is cutadapt 1.15 with Python 2.7.14+ Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC L79-Saccharomyces_paradoxus-CG_only.end1.36mers.fastq.gz Running on 1 core Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 242.99 s (24 us/read; 2.46 M reads/minute). === Summary === Total reads processed: 9,970,308 Reads with adapters: 5,396,006 (54.1%) Reads written (passing filters): 9,970,308 (100.0%) Total basepairs processed: 358,931,088 bp Quality-trimmed: 3,506,358 bp (1.0%) Total written (filtered): 333,294,443 bp (92.9%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 5396006 times. No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 57.5% C: 14.1% G: 1.5% T: 26.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 4101121 2492577.0 0 4101121 2 101865 623144.2 0 101865 3 71074 155786.1 0 71074 4 56857 38946.5 0 56857 5 47231 9736.6 0 47231 6 44490 2434.2 0 44490 7 42207 608.5 0 42207 8 41371 152.1 0 41371 9 43386 38.0 0 43080 306 10 54707 9.5 1 47213 7494 11 39584 2.4 1 31766 7818 12 47135 0.6 1 39522 7613 13 55516 0.1 1 46407 9109 14 38203 0.1 1 30384 7819 15 56483 0.1 1 46446 10037 16 35849 0.1 1 28707 7142 17 49015 0.1 1 40121 8894 18 50156 0.1 1 41805 8351 19 48412 0.1 1 40260 8152 20 48876 0.1 1 40639 8237 21 49620 0.1 1 41311 8309 22 55415 0.1 1 47598 7817 23 45578 0.1 1 38696 6882 24 39579 0.1 1 33956 5623 25 24551 0.1 1 21036 3515 26 14966 0.1 1 12600 2366 27 11733 0.1 1 9557 2176 28 14605 0.1 1 11031 3574 29 19593 0.1 1 13344 6249 30 19786 0.1 1 10275 9511 31 12949 0.1 1 3880 9069 32 10550 0.1 1 1382 9168 33 3357 0.1 1 59 3298 34 159 0.1 1 0 159 35 21 0.1 1 0 21 36 6 0.1 1 0 6 RUN STATISTICS FOR INPUT FILE: L79-Saccharomyces_paradoxus-CG_only.end1.36mers.fastq.gz ============================================= 9970308 sequences processed in total