SUMMARISING RUN PARAMETERS ========================== Input filename: L78-Saccharomyces_paradoxus-GC+CG.end1.36mers.fastq.gz Trimming mode: paired-end Trim Galore version: 0.4.4 Cutadapt version: 1.15 Quality Phred score cutoff: 20 Quality encoding type selected: ASCII+33 Adapter sequence: 'AGATCGGAAGAGC' (Illumina TruSeq, Sanger iPCR; auto-detected) Maximum trimming error rate: 0.1 (default) Minimum required adapter overlap (stringency): 1 bp Minimum required sequence length for both reads before a sequence pair gets removed: 20 bp All sequences will be trimmed by 1 bp on their 3' end to avoid problems with invalid paired-end alignments with Bowtie 1 Output file will be GZIP compressed This is cutadapt 1.15 with Python 2.7.14+ Command line parameters: -f fastq -e 0.1 -q 20 -O 1 -a AGATCGGAAGAGC L78-Saccharomyces_paradoxus-GC+CG.end1.36mers.fastq.gz Running on 1 core Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 200.74 s (23 us/read; 2.66 M reads/minute). === Summary === Total reads processed: 8,886,861 Reads with adapters: 4,962,263 (55.8%) Reads written (passing filters): 8,886,861 (100.0%) Total basepairs processed: 319,926,996 bp Quality-trimmed: 3,210,851 bp (1.0%) Total written (filtered): 292,834,953 bp (91.5%) === Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 4962263 times. No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 58.2% C: 12.9% G: 1.7% T: 27.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 1 3491671 2221715.2 0 3491671 2 131593 555428.8 0 131593 3 75398 138857.2 0 75398 4 61850 34714.3 0 61850 5 51294 8678.6 0 51294 6 48586 2169.6 0 48586 7 46208 542.4 0 46208 8 45664 135.6 0 45664 9 48293 33.9 0 48000 293 10 60843 8.5 1 52405 8438 11 44016 2.1 1 35014 9002 12 52878 0.5 1 44143 8735 13 62793 0.1 1 52292 10501 14 43084 0.1 1 34382 8702 15 63999 0.1 1 52742 11257 16 40819 0.1 1 32699 8120 17 56978 0.1 1 46712 10266 18 56364 0.1 1 46871 9493 19 54764 0.1 1 45356 9408 20 56140 0.1 1 46413 9727 21 56535 0.1 1 47058 9477 22 66421 0.1 1 56961 9460 23 54932 0.1 1 46625 8307 24 49225 0.1 1 42101 7124 25 30563 0.1 1 26230 4333 26 17847 0.1 1 15020 2827 27 13474 0.1 1 11024 2450 28 15364 0.1 1 11821 3543 29 20792 0.1 1 14425 6367 30 18843 0.1 1 10466 8377 31 12833 0.1 1 4723 8110 32 9030 0.1 1 1770 7260 33 3095 0.1 1 87 3008 34 54 0.1 1 0 54 35 12 0.1 1 1 11 36 8 0.1 1 2 6 RUN STATISTICS FOR INPUT FILE: L78-Saccharomyces_paradoxus-GC+CG.end1.36mers.fastq.gz ============================================= 8886861 sequences processed in total